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Protein

E3 ubiquitin-protein ligase HECTD3

Gene

HECTD3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

E3 ubiquitin ligases accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Mediates ubiquitination of TRIOBP and its subsequent proteasomal degradation, thus faciliting cell cycle progression by regulating the turn-over of TRIOBP. Mediates also ubiquitination of STX8 (By similarity).By similarity1 Publication

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei823 – 8231Glycyl thioester intermediate

GO - Molecular functioni

  • ligase activity Source: UniProtKB-KW
  • ubiquitin-protein transferase activity Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Ubl conjugation pathway

Enzyme and pathway databases

BRENDAi2.3.2.B9. 2681.
6.3.2.19. 2681.
ReactomeiR-HSA-983168. Antigen processing: Ubiquitination & Proteasome degradation.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase HECTD3 (EC:6.3.2.-)
Alternative name(s):
HECT domain-containing protein 3
Gene namesi
Name:HECTD3
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

HGNCiHGNC:26117. HECTD3.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi823 – 8231C → A: Loss of ubiquitin-ligase activity. 1 Publication

Organism-specific databases

PharmGKBiPA142671698.

Polymorphism and mutation databases

BioMutaiHECTD3.
DMDMi74744877.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedCombined sources
Chaini2 – 861860E3 ubiquitin-protein ligase HECTD3PRO_0000241445Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineCombined sources
Modified residuei12 – 121PhosphoserineCombined sources

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ5T447.
MaxQBiQ5T447.
PaxDbiQ5T447.
PeptideAtlasiQ5T447.
PRIDEiQ5T447.

PTM databases

iPTMnetiQ5T447.
PhosphoSiteiQ5T447.

Expressioni

Gene expression databases

BgeeiENSG00000126107.
CleanExiHS_HECTD3.
GenevisibleiQ5T447. HS.

Organism-specific databases

HPAiHPA027467.
HPA054302.

Interactioni

Subunit structurei

Interacts with TRIOBP. Interacts with STX8 (By similarity).By similarity

Protein-protein interaction databases

BioGridi122781. 45 interactions.
IntActiQ5T447. 4 interactions.
STRINGi9606.ENSP00000361245.

Structurei

3D structure databases

ProteinModelPortaliQ5T447.
SMRiQ5T447. Positions 232-370, 583-848.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini219 – 397179DOCPROSITE-ProRule annotationAdd
BLAST
Domaini512 – 857346HECTPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 DOC domain.PROSITE-ProRule annotation
Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0939. Eukaryota.
COG5021. LUCA.
GeneTreeiENSGT00840000129735.
HOGENOMiHOG000007274.
HOVERGENiHBG058297.
InParanoidiQ5T447.
KOiK12233.
OMAiQPAEAYT.
OrthoDBiEOG091G01ET.
PhylomeDBiQ5T447.

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
InterProiIPR004939. APC_su10/DOC_dom.
IPR008979. Galactose-bd-like.
IPR000569. HECT_dom.
[Graphical view]
PfamiPF03256. ANAPC10. 1 hit.
PF00632. HECT. 1 hit.
[Graphical view]
SMARTiSM01337. APC10. 1 hit.
SM00119. HECTc. 1 hit.
[Graphical view]
SUPFAMiSSF49785. SSF49785. 1 hit.
SSF56204. SSF56204. 1 hit.
PROSITEiPS51284. DOC. 1 hit.
PS50237. HECT. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q5T447-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAGPGPGAVL ESPRQLLGRV RFLAEAARSL RAGRPLPAAL AFVPREVLYK
60 70 80 90 100
LYKDPAGPSR VLLPVWEAEG LGLRVGAAGP APGTGSGPLR AARDSIELRR
110 120 130 140 150
GACVRTTGEE LCNGHGLWVK LTKEQLAEHL GDCGLQEGWL LVCRPAEGGA
160 170 180 190 200
RLVPIDTPNH LQRQQQLFGV DYRPVLRWEQ VVDLTYSHRL GSRPQPAEAY
210 220 230 240 250
AEAVQRLLYV PPTWTYECDE DLIHFLYDHL GKEDENLGSV KQYVESIDVS
260 270 280 290 300
SYTEEFNVSC LTDSNADTYW ESDGSQCQHW VRLTMKKGTI VKKLLLTVDT
310 320 330 340 350
TDDNFMPKRV VVYGGEGDNL KKLSDVSIDE TLIGDVCVLE DMTVHLPIIE
360 370 380 390 400
IRIVECRDDG IDVRLRGVKI KSSRQRELGL NADLFQPTSL VRYPRLEGTD
410 420 430 440 450
PEVLYRRAVL LQRFIKILDS VLHHLVPAWD HTLGTFSEIK QVKQFLLLSR
460 470 480 490 500
QRPGLVAQCL RDSESSKPSF MPRLYINRRL AMEHRACPSR DPACKNAVFT
510 520 530 540 550
QVYEGLKPSD KYEKPLDYRW PMRYDQWWEC KFIAEGIIDQ GGGFRDSLAD
560 570 580 590 600
MSEELCPSSA DTPVPLPFFV RTANQGNGTG EARDMYVPNP SCRDFAKYEW
610 620 630 640 650
IGQLMGAALR GKEFLVLALP GFVWKQLSGE EVSWSKDFPA VDSVLVKLLE
660 670 680 690 700
VMEGMDKETF EFKFGKELTF TTVLSDQQVV ELIPGGAGIV VGYGDRSRFI
710 720 730 740 750
QLVQKARLEE SKEQVAAMQA GLLKVVPQAV LDLLTWQELE KKVCGDPEVT
760 770 780 790 800
VDALRKLTRF EDFEPSDSRV QYFWEALNNF TNEDRSRFLR FVTGRSRLPA
810 820 830 840 850
RIYIYPDKLG YETTDALPES STCSSTLFLP HYASAKVCEE KLRYAAYNCV
860
AIDTDMSPWE E
Length:861
Mass (Da):97,113
Last modified:December 21, 2004 - v1
Checksum:i91009CE9FB84F4E7
GO
Isoform 2 (identifier: Q5T447-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-390: Missing.
     391-412: VRYPRLEGTDPEVLYRRAVLLQ → MLGSWGCYRYAKLFSCSLSTHA

Note: No experimental confirmation available.
Show »
Length:471
Mass (Da):53,688
Checksum:iB817DE3D89449FE9
GO

Sequence cautioni

The sequence BAB15015 differs from that shown.Contaminating sequence. Sequence of unknown origin in the N-terminal part.Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 390390Missing in isoform 2. 1 PublicationVSP_019439Add
BLAST
Alternative sequencei391 – 41222VRYPR…AVLLQ → MLGSWGCYRYAKLFSCSLST HA in isoform 2. 1 PublicationVSP_019440Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK024809 mRNA. Translation: BAB15015.1. Sequence problems.
AK056873 mRNA. Translation: BAG51819.1.
AK091583 mRNA. Translation: BAG52386.1.
AL359473 Genomic DNA. Translation: CAI16437.1.
AL359473 Genomic DNA. Translation: CAI16438.1.
CH471059 Genomic DNA. Translation: EAX07010.1.
BC019105 mRNA. Translation: AAH19105.2.
CCDSiCCDS41318.1. [Q5T447-1]
RefSeqiNP_078878.3. NM_024602.5. [Q5T447-1]
UniGeneiHs.525084.

Genome annotation databases

EnsembliENST00000372168; ENSP00000361241; ENSG00000126107. [Q5T447-2]
ENST00000372172; ENSP00000361245; ENSG00000126107. [Q5T447-1]
GeneIDi79654.
KEGGihsa:79654.
UCSCiuc001cmy.5. human. [Q5T447-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK024809 mRNA. Translation: BAB15015.1. Sequence problems.
AK056873 mRNA. Translation: BAG51819.1.
AK091583 mRNA. Translation: BAG52386.1.
AL359473 Genomic DNA. Translation: CAI16437.1.
AL359473 Genomic DNA. Translation: CAI16438.1.
CH471059 Genomic DNA. Translation: EAX07010.1.
BC019105 mRNA. Translation: AAH19105.2.
CCDSiCCDS41318.1. [Q5T447-1]
RefSeqiNP_078878.3. NM_024602.5. [Q5T447-1]
UniGeneiHs.525084.

3D structure databases

ProteinModelPortaliQ5T447.
SMRiQ5T447. Positions 232-370, 583-848.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi122781. 45 interactions.
IntActiQ5T447. 4 interactions.
STRINGi9606.ENSP00000361245.

PTM databases

iPTMnetiQ5T447.
PhosphoSiteiQ5T447.

Polymorphism and mutation databases

BioMutaiHECTD3.
DMDMi74744877.

Proteomic databases

EPDiQ5T447.
MaxQBiQ5T447.
PaxDbiQ5T447.
PeptideAtlasiQ5T447.
PRIDEiQ5T447.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000372168; ENSP00000361241; ENSG00000126107. [Q5T447-2]
ENST00000372172; ENSP00000361245; ENSG00000126107. [Q5T447-1]
GeneIDi79654.
KEGGihsa:79654.
UCSCiuc001cmy.5. human. [Q5T447-1]

Organism-specific databases

CTDi79654.
GeneCardsiHECTD3.
H-InvDBHIX0199838.
HGNCiHGNC:26117. HECTD3.
HPAiHPA027467.
HPA054302.
neXtProtiNX_Q5T447.
PharmGKBiPA142671698.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0939. Eukaryota.
COG5021. LUCA.
GeneTreeiENSGT00840000129735.
HOGENOMiHOG000007274.
HOVERGENiHBG058297.
InParanoidiQ5T447.
KOiK12233.
OMAiQPAEAYT.
OrthoDBiEOG091G01ET.
PhylomeDBiQ5T447.

Enzyme and pathway databases

UniPathwayiUPA00143.
BRENDAi2.3.2.B9. 2681.
6.3.2.19. 2681.
ReactomeiR-HSA-983168. Antigen processing: Ubiquitination & Proteasome degradation.

Miscellaneous databases

GenomeRNAii79654.
PROiQ5T447.

Gene expression databases

BgeeiENSG00000126107.
CleanExiHS_HECTD3.
GenevisibleiQ5T447. HS.

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
InterProiIPR004939. APC_su10/DOC_dom.
IPR008979. Galactose-bd-like.
IPR000569. HECT_dom.
[Graphical view]
PfamiPF03256. ANAPC10. 1 hit.
PF00632. HECT. 1 hit.
[Graphical view]
SMARTiSM01337. APC10. 1 hit.
SM00119. HECTc. 1 hit.
[Graphical view]
SUPFAMiSSF49785. SSF49785. 1 hit.
SSF56204. SSF56204. 1 hit.
PROSITEiPS51284. DOC. 1 hit.
PS50237. HECT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiHECD3_HUMAN
AccessioniPrimary (citable) accession number: Q5T447
Secondary accession number(s): B3KPV7
, B3KRH4, Q5T448, Q9H783
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 27, 2006
Last sequence update: December 21, 2004
Last modified: September 7, 2016
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.