Reviewed, UniProtKB/Swiss-Prot Q5T2L2 (AKCL1_HUMAN)

Names and origin

Protein names

Recommended name:
    Aldo-keto reductase family 1 member C-like protein 1
    EC=1.1.1.-

Gene names

Name:

AKR1CL1

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	302 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Subcellular location	Cytoplasm Potential.
Sequence similarities	Belongs to the aldo/keto reductase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Coding sequence diversity	Alternative splicing Polymorphism
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	oxidoreductase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q5T2L2-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q5T2L2-2)

The sequence of this isoform differs from the canonical sequence as follows:
     127-129: QPG → GSS
     130-302: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 302	302	Aldo-keto reductase family 1 member C-like protein 1		PRO_0000287879
Regions
	Nucleotide binding	16 – 25	10	NADP By similarity
	Nucleotide binding	199 – 262	64	NADP By similarity
Sites
	Active site	58	1	Proton donor By similarity
	Binding site	120	1	Substrate By similarity
	Site	57	1	Important for substrate specificity By similarity
	Site	87	1	Lowers pKa of active site Tyr By similarity
	Site	209	1	Involved in ligand recognition and product release
Natural variations
	Alternative sequence	127 – 129	3	QPG → GSS in isoform 2.		VSP_025612
	Alternative sequence	130 – 302	173	Missing in isoform 2.		VSP_025613
	Natural variant	50	1	R → H: dbSNP rs7097295.		VAR_032355

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified December 21, 2004. Version 1. Checksum: BA14D656F6B655D7	FASTA	302	34,587
	10 20 30 40 50 60 MMTDLKQSHS VRLNDGPFMP VLGFGTYAPD HTPKSQAAEA TKVAIDVGFR HIDSAYLYQN 70 80 90 100 110 120 EEEVGQAIWE KIADGTVKRE EIFYTIKLWA TFFRAELVHP ALERSLKKLG PDYVDLFIIH 130 140 150 160 170 180 VPFAMKQPGK ELLPKDASGE IILETVELCD TWELELILNK PGLRYKPTCN QVVECHPYLN 190 200 210 220 230 240 QSKLLEFCKS KDIVLVAYSA LGSQRDPQWV DPDCPHLLEE PILKSIAKKH SRSPGQVALR 250 260 270 280 290 300 YQLQRGVVVL AKSFSQERIK ENFQIFDFEL TPEDMKAIDG LNRNLRYDNA ANHPYFPFSE EY
	Isoform 2. Checksum: EE42253565182210	FASTA	129	14,588
	10 20 30 40 50 60 MMTDLKQSHS VRLNDGPFMP VLGFGTYAPD HTPKSQAAEA TKVAIDVGFR HIDSAYLYQN 70 80 90 100 110 120 EEEVGQAIWE KIADGTVKRE EIFYTIKLWA TFFRAELVHP ALERSLKKLG PDYVDLFIIH VPFAMKGSS

References

[1]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Testis.
[2]	"The DNA sequence and comparative analysis of human chromosome 10." Deloukas P., Earthrowl M.E., Grafham D.V., Rubenfield M., French L., Steward C.A., Sims S.K., Jones M.C., Searle S., Scott C., Howe K., Hunt S.E., Andrews T.D., Gilbert J.G.R., Swarbreck D., Ashurst J.L., Taylor A., Battles J. , Bird C.P., Ainscough R., Almeida J.P., Ashwell R.I.S., Ambrose K.D., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Bates K., Beasley H., Bray-Allen S., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Cahill P., Camire D., Carter N.P., Chapman J.C., Clark S.Y., Clarke G., Clee C.M., Clegg S., Corby N., Coulson A., Dhami P., Dutta I., Dunn M., Faulkner L., Frankish A., Frankland J.A., Garner P., Garnett J., Gribble S., Griffiths C., Grocock R., Gustafson E., Hammond S., Harley J.L., Hart E., Heath P.D., Ho T.P., Hopkins B., Horne J., Howden P.J., Huckle E., Hynds C., Johnson C., Johnson D., Kana A., Kay M., Kimberley A.M., Kershaw J.K., Kokkinaki M., Laird G.K., Lawlor S., Lee H.M., Leongamornlert D.A., Laird G., Lloyd C., Lloyd D.M., Loveland J., Lovell J., McLaren S., McLay K.E., McMurray A., Mashreghi-Mohammadi M., Matthews L., Milne S., Nickerson T., Nguyen M., Overton-Larty E., Palmer S.A., Pearce A.V., Peck A.I., Pelan S., Phillimore B., Porter K., Rice C.M., Rogosin A., Ross M.T., Sarafidou T., Sehra H.K., Shownkeen R., Skuce C.D., Smith M., Standring L., Sycamore N., Tester J., Thorpe A., Torcasso W., Tracey A., Tromans A., Tsolas J., Wall M., Walsh J., Wang H., Weinstock K., West A.P., Willey D.L., Whitehead S.L., Wilming L., Wray P.W., Young L., Chen Y., Lovering R.C., Moschonas N.K., Siebert R., Fechtel K., Bentley D., Durbin R.M., Hubbard T., Doucette-Stamm L., Beck S., Smith D.R., Rogers J. Nature 429:375-381(2004) [PubMed: 15164054] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).

Cross-references

Sequence databases
EMBLGenBankDDBJ	AK131335 mRNA. Translation: BAD18494.1. AL391427, AL355303 Genomic DNA. Translation: CAI14727.1. AL355303, AL391427 Genomic DNA. Translation: CAI14201.1. BC101204 mRNA. Translation: AAI01205.1. BC101205 mRNA. Translation: AAI01206.1.
IPI	IPI00259851. IPI00745863.
RefSeq	NP_001007537.1.
UniGene	Hs.282513
3D structure databases
ModBase	Search...
Proteomic databases
PRIDE	Q5T2L2.
Genome annotation databases
Ensembl	ENSG00000196326. Homo sapiens. [Contig view]
GeneID	340811.
KEGG	hsa:340811.
UCSC	uc009xia.1. human.
Organism-specific databases
GeneCards	GC10M005192.
HGNC	HGNC:23469. AKR1CL1.
PharmGKB	PA134869740.
GenAtlas	Search...
Phylogenomic databases
HOVERGEN	Q5T2L2.
OMA	Q5T2L2. ANHPYFP.
Gene expression databases
Bgee	Q5T2L2.
CleanEx	HS_AKR1CL1.
Family and domain databases
InterPro	IPR001395. Aldo/ket_red. IPR018170. Aldo/ket_reductase_CS. [Graphical view]
Gene3D	G3DSA:3.20.20.100. Aldo/ket_red. 1 hit.
PANTHER	PTHR11732. Aldo/ket_red. 1 hit.
Pfam	PF00248. Aldo_ket_red. 1 hit. [Graphical view]
ProDom	PD000288. Aldo/ket_red. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00798. ALDOKETO_REDUCTASE_1. 1 hit. PS00062. ALDOKETO_REDUCTASE_2. False negative. PS00063. ALDOKETO_REDUCTASE_3. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	98023.

Entry information

Entry name

AKCL1_HUMAN

Accession

Primary (citable) accession number: Q5T2L2
Secondary accession number(s): A6NF66, Q6ZN81

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 15, 2007
Last sequence update:	December 21, 2004
Last modified:	July 7, 2009
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)