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Protein

OTU domain-containing protein 3

Gene

OTUD3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Deubiquitinating enzyme that hydrolyzes 'Lys-6'- and 'Lys-11'-linked polyubiquitin. Also hydrolyzes heterotypic (mixed and branched) and homotypic chains.1 Publication

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei73By similarity1
Active sitei76NucleophileBy similarity1
Active sitei182By similarity1

GO - Molecular functioni

  • thiol-dependent ubiquitin-specific protease activity Source: UniProtKB

GO - Biological processi

  • protein K11-linked deubiquitination Source: UniProtKB
  • protein K6-linked deubiquitination Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Ubl conjugation pathway

Enzyme and pathway databases

ReactomeiR-HSA-5689896. Ovarian tumor domain proteases.

Protein family/group databases

MEROPSiC85.003.

Names & Taxonomyi

Protein namesi
Recommended name:
OTU domain-containing protein 3 (EC:3.4.19.12)
Gene namesi
Name:OTUD3
Synonyms:KIAA0459
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

HGNCiHGNC:29038. OTUD3.

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi178 – 181RYGE → LSNG: Impaired activity. 1 Publication4

Organism-specific databases

DisGeNETi23252.
OpenTargetsiENSG00000169914.
PharmGKBiPA142671215.

Polymorphism and mutation databases

BioMutaiOTUD3.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000581031 – 398OTU domain-containing protein 3Add BLAST398

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei224PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ5T2D3.
MaxQBiQ5T2D3.
PaxDbiQ5T2D3.
PeptideAtlasiQ5T2D3.
PRIDEiQ5T2D3.

PTM databases

iPTMnetiQ5T2D3.
PhosphoSitePlusiQ5T2D3.

Expressioni

Gene expression databases

BgeeiENSG00000169914.
CleanExiHS_OTUD3.
GenevisibleiQ5T2D3. HS.

Organism-specific databases

HPAiHPA028543.
HPA028544.

Interactioni

Protein-protein interaction databases

BioGridi116856. 3 interactors.
DIPiDIP-61702N.
STRINGi9606.ENSP00000364261.

Structurei

Secondary structure

1398
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi56 – 62Combined sources7
Beta strandi65 – 68Combined sources4
Helixi76 – 86Combined sources11
Beta strandi87 – 89Combined sources3
Helixi92 – 105Combined sources14
Helixi107 – 110Combined sources4
Helixi111 – 113Combined sources3
Helixi120 – 128Combined sources9
Helixi136 – 145Combined sources10
Beta strandi149 – 153Combined sources5
Beta strandi160 – 166Combined sources7
Beta strandi172 – 178Combined sources7
Turni179 – 181Combined sources3
Beta strandi182 – 188Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4BOUX-ray1.55A52-209[»]
ProteinModelPortaliQ5T2D3.
SMRiQ5T2D3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini65 – 189OTUPROSITE-ProRule annotationAdd BLAST125
Domaini230 – 270UBA-likeAdd BLAST41

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni70 – 76Cys-loop7
Regioni127 – 137Variable-loopAdd BLAST11
Regioni177 – 182His-loop6

Domaini

The UBA-like domain has no influence on ubiquitin hydrolysis.1 Publication
Specificity is given by the S1' ubiquitin-binding site within the OTU domain composed of the Cys-, His- and Variable-loops.1 Publication

Sequence similaritiesi

Contains 1 OTU domain.PROSITE-ProRule annotation
Contains 1 UBA-like domain.Curated

Phylogenomic databases

eggNOGiENOG410IERN. Eukaryota.
ENOG4110S6T. LUCA.
GeneTreeiENSGT00390000016392.
HOGENOMiHOG000049263.
HOVERGENiHBG056738.
InParanoidiQ5T2D3.
KOiK13717.
OMAiQLPKVTN.
OrthoDBiEOG091G0CNS.
PhylomeDBiQ5T2D3.
TreeFamiTF329594.

Family and domain databases

InterProiIPR003323. OTU_dom.
[Graphical view]
PfamiPF02338. OTU. 1 hit.
[Graphical view]
PROSITEiPS50802. OTU. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5T2D3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSRKQAAKSR PGSGSRKAEA ERKRDERAAR RALAKERRNR PESGGGGGCE
60 70 80 90 100
EEFVSFANQL QALGLKLREV PGDGNCLFRA LGDQLEGHSR NHLKHRQETV
110 120 130 140 150
DYMIKQREDF EPFVEDDIPF EKHVASLAKP GTFAGNDAIV AFARNHQLNV
160 170 180 190 200
VIHQLNAPLW QIRGTEKSSV RELHIAYRYG EHYDSVRRIN DNSEAPAHLQ
210 220 230 240 250
TDFQMLHQDE SNKREKIKTK GMDSEDDLRD EVEDAVQKVC NATGCSDFNL
260 270 280 290 300
IVQNLEAENY NIESAIIAVL RMNQGKRNNA EENLEPSGRV LKQCGPLWEE
310 320 330 340 350
GGSGARIFGN QGLNEGRTEN NKAQASPSEE NKANKNQLAK VTNKQRREQQ
360 370 380 390
WMEKKKRQEE RHRHKALESR GSHRDNNRSE AEANTQVTLV KTFAALNI
Length:398
Mass (Da):45,124
Last modified:December 21, 2004 - v1
Checksum:i9C16E610CF1E3C5A
GO

Sequence cautioni

The sequence BAA32304 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_051258321N → S.Corresponds to variant rs2298110dbSNPEnsembl.1
Natural variantiVAR_051259333A → T.Corresponds to variant rs10916668dbSNPEnsembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB007928 mRNA. Translation: BAA32304.2. Different initiation.
AL391883 Genomic DNA. Translation: CAI17041.1.
CCDSiCCDS41279.1.
RefSeqiNP_056022.1. NM_015207.1.
UniGeneiHs.374987.

Genome annotation databases

EnsembliENST00000375120; ENSP00000364261; ENSG00000169914.
GeneIDi23252.
KEGGihsa:23252.
UCSCiuc001bcs.5. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB007928 mRNA. Translation: BAA32304.2. Different initiation.
AL391883 Genomic DNA. Translation: CAI17041.1.
CCDSiCCDS41279.1.
RefSeqiNP_056022.1. NM_015207.1.
UniGeneiHs.374987.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4BOUX-ray1.55A52-209[»]
ProteinModelPortaliQ5T2D3.
SMRiQ5T2D3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi116856. 3 interactors.
DIPiDIP-61702N.
STRINGi9606.ENSP00000364261.

Protein family/group databases

MEROPSiC85.003.

PTM databases

iPTMnetiQ5T2D3.
PhosphoSitePlusiQ5T2D3.

Polymorphism and mutation databases

BioMutaiOTUD3.

Proteomic databases

EPDiQ5T2D3.
MaxQBiQ5T2D3.
PaxDbiQ5T2D3.
PeptideAtlasiQ5T2D3.
PRIDEiQ5T2D3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000375120; ENSP00000364261; ENSG00000169914.
GeneIDi23252.
KEGGihsa:23252.
UCSCiuc001bcs.5. human.

Organism-specific databases

CTDi23252.
DisGeNETi23252.
GeneCardsiOTUD3.
HGNCiHGNC:29038. OTUD3.
HPAiHPA028543.
HPA028544.
MIMi611758. gene.
neXtProtiNX_Q5T2D3.
OpenTargetsiENSG00000169914.
PharmGKBiPA142671215.
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IERN. Eukaryota.
ENOG4110S6T. LUCA.
GeneTreeiENSGT00390000016392.
HOGENOMiHOG000049263.
HOVERGENiHBG056738.
InParanoidiQ5T2D3.
KOiK13717.
OMAiQLPKVTN.
OrthoDBiEOG091G0CNS.
PhylomeDBiQ5T2D3.
TreeFamiTF329594.

Enzyme and pathway databases

ReactomeiR-HSA-5689896. Ovarian tumor domain proteases.

Miscellaneous databases

GenomeRNAii23252.
PROiQ5T2D3.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000169914.
CleanExiHS_OTUD3.
GenevisibleiQ5T2D3. HS.

Family and domain databases

InterProiIPR003323. OTU_dom.
[Graphical view]
PfamiPF02338. OTU. 1 hit.
[Graphical view]
PROSITEiPS50802. OTU. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiOTUD3_HUMAN
AccessioniPrimary (citable) accession number: Q5T2D3
Secondary accession number(s): O75047
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 30, 2005
Last sequence update: December 21, 2004
Last modified: November 30, 2016
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.