ID   DCST2_HUMAN             Reviewed;         773 AA.
AC   Q5T1A1; Q2M2R2; Q8N810; Q96M03;
DT   06-MAR-2007, integrated into UniProtKB/Swiss-Prot.
DT   20-FEB-2007, sequence version 2.
DT   24-JAN-2024, entry version 125.
DE   RecName: Full=DC-STAMP domain-containing protein 2;
GN   Name=DCST2;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), AND NUCLEOTIDE SEQUENCE
RP   [LARGE SCALE MRNA] OF 170-773 (ISOFORM 1).
RC   TISSUE=Testis, and Thymus;
RX   PubMed=14702039; DOI=10.1038/ng1285;
RA   Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA   Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA   Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA   Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA   Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA   Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA   Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA   Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA   Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA   Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA   Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA   Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA   Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA   Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA   Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA   Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA   Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA   Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA   Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA   Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA   Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA   Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA   Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA   Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA   Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA   Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA   Isogai T., Sugano S.;
RT   "Complete sequencing and characterization of 21,243 full-length human
RT   cDNAs.";
RL   Nat. Genet. 36:40-45(2004).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=16710414; DOI=10.1038/nature04727;
RA   Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A.,
RA   Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C.,
RA   Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K.,
RA   Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C.,
RA   Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W.,
RA   Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J.,
RA   Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J.,
RA   Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y.,
RA   Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J.,
RA   Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H.,
RA   Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L.,
RA   Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J.,
RA   Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S.,
RA   Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K.,
RA   Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R.,
RA   Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M.,
RA   Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S.,
RA   Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J.,
RA   Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W.,
RA   McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N.,
RA   Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V.,
RA   Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J.,
RA   Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E.,
RA   Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S.,
RA   Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M.,
RA   White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H.,
RA   Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E.,
RA   Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G.,
RA   Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.;
RT   "The DNA sequence and biological annotation of human chromosome 1.";
RL   Nature 441:315-321(2006).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-744 (ISOFORM 1).
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
CC   -!- FUNCTION: Essential sperm cell-surface protein required for sperm-egg
CC       fusion and fertilization. {ECO:0000250|UniProtKB:A0A140LIJ0}.
CC   -!- SUBUNIT: Interacts with DCST1. {ECO:0000250|UniProtKB:A0A140LIJ0}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasmic vesicle, secretory vesicle, acrosome
CC       membrane {ECO:0000250|UniProtKB:A0A140LIJ0}; Multi-pass membrane
CC       protein {ECO:0000255}. Note=Localizes in the anterior acrosome before
CC       the acrosome reaction and then translocates to the equatorial segment
CC       in acrosome-reacted sperm. {ECO:0000250|UniProtKB:A0A140LIJ0}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=1;
CC         IsoId=Q5T1A1-1; Sequence=Displayed;
CC       Name=2;
CC         IsoId=Q5T1A1-2; Sequence=VSP_023408, VSP_023409;
CC   -!- SEQUENCE CAUTION:
CC       Sequence=BAC05065.1; Type=Frameshift; Evidence={ECO:0000305};
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DR   EMBL; AK057496; BAB71511.1; -; mRNA.
DR   EMBL; AK097468; BAC05065.1; ALT_SEQ; mRNA.
DR   EMBL; AL451085; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; BC111794; AAI11795.1; -; mRNA.
DR   CCDS; CCDS1082.2; -. [Q5T1A1-1]
DR   RefSeq; NP_653223.2; NM_144622.2. [Q5T1A1-1]
DR   AlphaFoldDB; Q5T1A1; -.
DR   BioGRID; 126069; 2.
DR   STRING; 9606.ENSP00000357409; -.
DR   GlyCosmos; Q5T1A1; 3 sites, No reported glycans.
DR   GlyGen; Q5T1A1; 3 sites.
DR   iPTMnet; Q5T1A1; -.
DR   PhosphoSitePlus; Q5T1A1; -.
DR   BioMuta; DCST2; -.
DR   DMDM; 143975954; -.
DR   MassIVE; Q5T1A1; -.
DR   PaxDb; 9606-ENSP00000357409; -.
DR   PeptideAtlas; Q5T1A1; -.
DR   ProteomicsDB; 64250; -. [Q5T1A1-1]
DR   ProteomicsDB; 64251; -. [Q5T1A1-2]
DR   Antibodypedia; 68362; 11 antibodies from 6 providers.
DR   DNASU; 127579; -.
DR   Ensembl; ENST00000368424.4; ENSP00000357409.3; ENSG00000163354.15. [Q5T1A1-1]
DR   Ensembl; ENST00000485982.5; ENSP00000436964.1; ENSG00000163354.15. [Q5T1A1-2]
DR   GeneID; 127579; -.
DR   KEGG; hsa:127579; -.
DR   MANE-Select; ENST00000368424.4; ENSP00000357409.3; NM_144622.3; NP_653223.2.
DR   UCSC; uc001fgm.4; human. [Q5T1A1-1]
DR   AGR; HGNC:26562; -.
DR   CTD; 127579; -.
DR   DisGeNET; 127579; -.
DR   GeneCards; DCST2; -.
DR   HGNC; HGNC:26562; DCST2.
DR   HPA; ENSG00000163354; Tissue enhanced (retina, skin).
DR   MIM; 619861; gene.
DR   neXtProt; NX_Q5T1A1; -.
DR   OpenTargets; ENSG00000163354; -.
DR   PharmGKB; PA142672006; -.
DR   VEuPathDB; HostDB:ENSG00000163354; -.
DR   eggNOG; KOG3726; Eukaryota.
DR   GeneTree; ENSGT00940000153269; -.
DR   HOGENOM; CLU_015030_2_0_1; -.
DR   InParanoid; Q5T1A1; -.
DR   OMA; DDAKDNC; -.
DR   OrthoDB; 5400067at2759; -.
DR   PhylomeDB; Q5T1A1; -.
DR   TreeFam; TF318254; -.
DR   PathwayCommons; Q5T1A1; -.
DR   BioGRID-ORCS; 127579; 14 hits in 1142 CRISPR screens.
DR   GenomeRNAi; 127579; -.
DR   Pharos; Q5T1A1; Tdark.
DR   PRO; PR:Q5T1A1; -.
DR   Proteomes; UP000005640; Chromosome 1.
DR   RNAct; Q5T1A1; Protein.
DR   Bgee; ENSG00000163354; Expressed in right testis and 91 other cell types or tissues.
DR   ExpressionAtlas; Q5T1A1; baseline and differential.
DR   GO; GO:0002080; C:acrosomal membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0007342; P:fusion of sperm to egg plasma membrane involved in single fertilization; ISS:UniProtKB.
DR   GO; GO:0035036; P:sperm-egg recognition; ISS:UniProtKB.
DR   InterPro; IPR012858; DC_STAMP-like.
DR   PANTHER; PTHR21041:SF6; DC-STAMP DOMAIN-CONTAINING PROTEIN 2; 1.
DR   PANTHER; PTHR21041; DENDRITIC CELL-SPECIFIC TRANSMEMBRANE PROTEIN; 1.
DR   Pfam; PF07782; DC_STAMP; 1.
DR   Genevisible; Q5T1A1; HS.
PE   2: Evidence at transcript level;
KW   Alternative splicing; Cytoplasmic vesicle; Glycoprotein; Membrane;
KW   Reference proteome; Transmembrane; Transmembrane helix.
FT   CHAIN           1..773
FT                   /note="DC-STAMP domain-containing protein 2"
FT                   /id="PRO_0000278826"
FT   TOPO_DOM        1..26
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        27..47
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        48..51
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        52..72
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        73..233
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        234..254
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        255..322
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        323..343
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        344..415
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        416..436
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        437..499
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        500..520
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        521..773
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   REGION          692..773
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        735..750
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        754..773
FT                   /note="Pro residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   CARBOHYD        284
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        296
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        480
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   VAR_SEQ         625..639
FT                   /note="LYCLTCFRLLDNTCS -> FICKWHCSSSLVCTP (in isoform 2)"
FT                   /evidence="ECO:0000303|PubMed:14702039"
FT                   /id="VSP_023408"
FT   VAR_SEQ         640..773
FT                   /note="Missing (in isoform 2)"
FT                   /evidence="ECO:0000303|PubMed:14702039"
FT                   /id="VSP_023409"
SQ   SEQUENCE   773 AA;  86230 MW;  5B48863229DFAB36 CRC64;
     MPKVMKDVVH PLGGEEPSMA RAVVRSVGGF TLGLSLATAY GLLELLVEGH SPWGCLVGTL
     TLAAFLSLGM GFSRQVRATV LLLLPQAFSR QGRTLLLVAA FGLVLQGPCA NTLRNFTRAS
     EAVACGAELA LNQTAEVLQR AKQPLVSALN KIKAIARKTK EVADRVRKFF RSIMDGVKHI
     ARALRNVWQW LLHIGDVCNS ELGNPYLKCA RVFDDAKDSC MMVIPQAYHL CYVLMPFKLA
     LCGLASLVQV FCVIPKYIQP FLRQTIGTPV IQLLNRVRQE FEFNMTATHH FSVDLNASRS
     LSQVAMDLHE AVSMKLHRVR EALALMGFTT PLLLVLLYLQ ALFYRYCYLN WDHYDNIYIT
     SRFLRMEAVR STAGLPTVLP LSAHEARRYI PPGSIFLSQW EKFFYILETF NLIRHLLLVL
     FLVFLDYAVF WVLDLARHQL QGEIVARSPV LVSLTVEGTG YAGNIYRDLV SAFDVLQQGN
     ISILSRRCLL RPSEPDSTGY IVIGVMYGLC FFITLFGSYV SRLRRVICAS YYPSREQERI
     SYLYNVLLSR RTNLLAALHR SVRRRAADQG HRSAFLVLAS RCPCLGPFVS HFWLHQAYCL
     GCGQPQDEGD MENTVSCSTP GCQGLYCLTC FRLLDNTCSV CASPLSYQGD LDLELDSSDE
     EGPQLWLAAA QRKDPEQAWL LQQQLQEVLG RSLSMESTSE SSDLDEEKGP QQRKHGQQPL
     PEAHQPVSIL TSPEPHRPPE TSSATKGAPT PASEPSVPLS PPSLPDPSHP PPK
//