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Protein

E3 ubiquitin-protein ligase DCST1

Gene

DCST1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

E3 ubiquitin-protein ligase which mediates 'Lys-48'-linked ubiquitination of STAT2 and induces its proteosomal degradation thereby negatively regulating type-I-interferon signaling.1 Publication

Catalytic activityi

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[acceptor protein]-L-lysine.1 Publication

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.1 Publication
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri646 – 685RING-type; degeneratePROSITE-ProRule annotationAdd BLAST40

GO - Molecular functioni

  • metal ion binding Source: UniProtKB-KW
  • ubiquitin protein ligase activity Source: UniProtKB

GO - Biological processi

  • innate immune response Source: UniProtKB-KW
  • negative regulation of type I interferon-mediated signaling pathway Source: UniProtKB
  • protein ubiquitination involved in ubiquitin-dependent protein catabolic process Source: UniProtKB

Keywordsi

Molecular functionTransferase
Biological processImmunity, Innate immunity, Ubl conjugation pathway
LigandMetal-binding, Zinc

Enzyme and pathway databases

UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase DCST1Curated (EC:2.3.2.271 Publication)
Alternative name(s):
DC-STAMP domain-containing protein 1
RING-type E3 ubiquitin transferaseCurated
Gene namesi
Name:DCST1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

EuPathDBiHostDB:ENSG00000163357.10.
HGNCiHGNC:26539. DCST1.
neXtProtiNX_Q5T197.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 48CytoplasmicSequence analysisAdd BLAST48
Transmembranei49 – 69HelicalSequence analysisAdd BLAST21
Topological domaini70 – 79ExtracellularSequence analysis10
Transmembranei80 – 100HelicalSequence analysisAdd BLAST21
Topological domaini101 – 115CytoplasmicSequence analysisAdd BLAST15
Transmembranei116 – 136HelicalSequence analysisAdd BLAST21
Topological domaini137 – 372ExtracellularSequence analysisAdd BLAST236
Transmembranei373 – 393HelicalSequence analysisAdd BLAST21
Topological domaini394 – 463CytoplasmicSequence analysisAdd BLAST70
Transmembranei464 – 484HelicalSequence analysisAdd BLAST21
Topological domaini485 – 553ExtracellularSequence analysisAdd BLAST69
Transmembranei554 – 574HelicalSequence analysisAdd BLAST21
Topological domaini575 – 706CytoplasmicSequence analysisAdd BLAST132

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Organism-specific databases

OpenTargetsiENSG00000163357.
PharmGKBiPA142672005.

Polymorphism and mutation databases

BioMutaiDCST1.
DMDMi74756229.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002788241 – 706E3 ubiquitin-protein ligase DCST1Add BLAST706

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi156N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi189N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi321N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi349N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi525N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ5T197.
PeptideAtlasiQ5T197.
PRIDEiQ5T197.

PTM databases

iPTMnetiQ5T197.
PhosphoSitePlusiQ5T197.

Expressioni

Inductioni

Induced by IFNB1.1 Publication

Gene expression databases

BgeeiENSG00000163357.
CleanExiHS_DCST1.
ExpressionAtlasiQ5T197. baseline and differential.
GenevisibleiQ5T197. HS.

Organism-specific databases

HPAiHPA060314.

Interactioni

Subunit structurei

Interacts with STAT2; the interaction results in STAT2 'Lys-48'-linked ubiquitination leading to its proteasomal degradation.1 Publication

Protein-protein interaction databases

BioGridi127192. 3 interactors.

Structurei

3D structure databases

ProteinModelPortaliQ5T197.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

The RING-type zinc finger domain is responsible for E3 ubiquitin ligase activity.1 Publication

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri646 – 685RING-type; degeneratePROSITE-ProRule annotationAdd BLAST40

Keywords - Domaini

Transmembrane, Transmembrane helix, Zinc-finger

Phylogenomic databases

eggNOGiKOG3726. Eukaryota.
ENOG410ZWQR. LUCA.
GeneTreeiENSGT00730000110972.
HOGENOMiHOG000112180.
HOVERGENiHBG081422.
InParanoidiQ5T197.
KOiK22375.
OMAiIYISTYF.
OrthoDBiEOG091G0GX3.
PhylomeDBiQ5T197.
TreeFamiTF318254.

Family and domain databases

InterProiView protein in InterPro
IPR012858. DC_STAMP-like.
IPR001841. Znf_RING.
PfamiView protein in Pfam
PF07782. DC_STAMP. 1 hit.
PROSITEiView protein in PROSITE
PS50089. ZF_RING_2. 1 hit.

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q5T197-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDIKHHQNGT RGQRRKQPHT TVQRLLTWGL PVSCSWFLWR QPGEFPVTAL
60 70 80 90 100
LLGAGAGGLL AIGLFQLLVN PMNIYEEQKI MFLYSLVGLG AMGWGTSPHI
110 120 130 140 150
RCASLLLVPK MLGKEGRLFV LGYALAAIYV GPVANLRHNL NNVIASLGCT
160 170 180 190 200
VELQINNTRA AWRISTAPLR AMFKDLLSSK ELLRAETRNI SATFEDLDAQ
210 220 230 240 250
VNSETGYTPE DTMDSGETAQ GREARQAPAS RLHLSTQKMY ELKTKLRCSY
260 270 280 290 300
VVNQAILSCR RWFDRKHEQC MKHIWVPLLT HLLCLPMKFK FFCGIAKVME
310 320 330 340 350
VWCRNRIPVE GNFGQTYDSL NQSIRGLDGE FSANIDFKEE KQAGVLGLNT
360 370 380 390 400
SWERVSTEVR DYVYRQEARL EWALGLLHVL LSCTFLLVLH ASFSYMDSYN
410 420 430 440 450
HDIRFDNIYI STYFCQIDDR RKKLGKRTLL PLRKAEEKTV IFPCKPTIQA
460 470 480 490 500
SEMSNVVREL LETLPILLLL VVLCGLDWAL YSIFDTIRHH SFLQYSFRSS
510 520 530 540 550
HKLEVKVGGD SMLARLLRKT IGALNTSSET VMESNNMPCL PQPVGLDARA
560 570 580 590 600
YWRAAVPIGL LVCLCLLQAF GYRLRRVIAA FYFPKREKKR ILFLYNDLLK
610 620 630 640 650
KRAAFTKLRR AAILRRERQQ KAPRHPLADI LHRGCPLLRR WLCRRCVVCQ
660 670 680 690 700
APETPESYVC RTLDCEAVYC WSCWDDMRQR CPVCTPREEL SSSAFSDSND

DTAYAG
Length:706
Mass (Da):80,712
Last modified:December 21, 2004 - v1
Checksum:iFAE1ADED291F846C
GO
Isoform 2 (identifier: Q5T197-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     624-664: RHPLADILHR...PESYVCRTLD → VFTEPQFLPL...SGVGLRCQRA
     665-706: Missing.

Note: No experimental confirmation available.
Show »
Length:664
Mass (Da):75,737
Checksum:iABE64344EFF240D5
GO
Isoform 3 (identifier: Q5T197-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     63-87: Missing.

Note: No experimental confirmation available.
Show »
Length:681
Mass (Da):77,726
Checksum:i1C5D9CE64FE91D70
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti467L → P in BAB71440 (PubMed:14702039).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_056859158T → I. Corresponds to variant dbSNP:rs9427170Ensembl.1
Natural variantiVAR_030868512M → L2 PublicationsCorresponds to variant dbSNP:rs11264300Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_04561963 – 87Missing in isoform 3. 1 PublicationAdd BLAST25
Alternative sequenceiVSP_023406624 – 664RHPLA…CRTLD → VFTEPQFLPLKNGKTGEICK ACSLEISLEWWSGVGLRCQR A in isoform 2. 1 PublicationAdd BLAST41
Alternative sequenceiVSP_023407665 – 706Missing in isoform 2. 1 PublicationAdd BLAST42

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK057347 mRNA. Translation: BAB71440.1.
AK301876 mRNA. Translation: BAG63312.1.
AL451085 Genomic DNA. No translation available.
BC064844 mRNA. Translation: AAH64844.1.
AF314227 Genomic DNA. Translation: AAS46286.1.
CCDSiCCDS1083.1. [Q5T197-1]
CCDS44235.1. [Q5T197-3]
RefSeqiNP_001137159.1. NM_001143687.2. [Q5T197-3]
NP_689707.2. NM_152494.3. [Q5T197-1]
UniGeneiHs.567717.

Genome annotation databases

EnsembliENST00000295542; ENSP00000295542; ENSG00000163357. [Q5T197-1]
ENST00000368419; ENSP00000357404; ENSG00000163357. [Q5T197-2]
ENST00000423025; ENSP00000387369; ENSG00000163357. [Q5T197-3]
GeneIDi149095.
KEGGihsa:149095.
UCSCiuc001fgn.3. human. [Q5T197-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Entry informationi

Entry nameiDCST1_HUMAN
AccessioniPrimary (citable) accession number: Q5T197
Secondary accession number(s): B4DXA0
, E9PHV3, Q5T198, Q6P1W6, Q71S70, Q96M70
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 6, 2007
Last sequence update: December 21, 2004
Last modified: March 28, 2018
This is version 116 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome