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Protein

Formin-binding protein 1-like

Gene

FNBP1L

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required to coordinate membrane tubulation with reorganization of the actin cytoskeleton during endocytosis. May bind to lipids such as phosphatidylinositol 4,5-bisphosphate and phosphatidylserine and promote membrane invagination and the formation of tubules. Also promotes CDC42-induced actin polymerization by activating the WASL/N-WASP-WASPIP/WIP complex, the predominant form of WASL/N-WASP in cells. Actin polymerization may promote the fission of membrane tubules to form endocytic vesicles. Essential for autophagy of intracellular bacterial pathogens.3 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei165Mediates end-to-end attachment of dimersBy similarity1

GO - Molecular functioni

GO - Biological processi

  • autophagy Source: UniProtKB-KW
  • cilium assembly Source: MGI
  • clathrin-dependent endocytosis Source: BHF-UCL
  • membrane budding Source: BHF-UCL
  • membrane invagination Source: BHF-UCL
  • membrane tubulation Source: BHF-UCL
  • positive regulation of filopodium assembly Source: BHF-UCL
  • vesicle organization Source: BHF-UCL
  • vesicle transport along actin filament Source: BHF-UCL
Complete GO annotation...

Keywords - Biological processi

Autophagy, Endocytosis

Keywords - Ligandi

Lipid-binding

Enzyme and pathway databases

ReactomeiR-HSA-8856828. Clathrin-mediated endocytosis.

Names & Taxonomyi

Protein namesi
Recommended name:
Formin-binding protein 1-like
Alternative name(s):
Transducer of Cdc42-dependent actin assembly protein 1
Short name:
Toca-1
Gene namesi
Name:FNBP1L
Synonyms:C1orf39, TOCA1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

HGNCiHGNC:20851. FNBP1L.

Subcellular locationi

GO - Cellular componenti

  • cell-cell adherens junction Source: BHF-UCL
  • cell cortex Source: UniProtKB-SubCell
  • cytoplasm Source: MGI
  • cytoplasmic, membrane-bounded vesicle Source: BHF-UCL
  • cytoskeleton Source: UniProtKB-SubCell
  • extracellular exosome Source: UniProtKB
  • plasma membrane Source: UniProtKB-SubCell
  • trans-Golgi network Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Cytoplasmic vesicle, Cytoskeleton, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi441 – 443MGD → IST: Impairs interaction with CDC42 and reduces CDC42-induced actin assembly. 1 Publication3
Mutagenesisi576W → K: Impairs interaction with WASL and reduces CDC42-induced actin assembly. 1 Publication1

Organism-specific databases

DisGeNETi54874.
OpenTargetsiENSG00000137942.
PharmGKBiPA128394675.

Polymorphism and mutation databases

BioMutaiFNBP1L.
DMDMi118572313.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002614341 – 605Formin-binding protein 1-likeAdd BLAST605

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei295PhosphoserineCombined sources1
Modified residuei488PhosphoserineCombined sources1
Modified residuei501PhosphoserineCombined sources1
Modified residuei505PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ5T0N5.
MaxQBiQ5T0N5.
PaxDbiQ5T0N5.
PeptideAtlasiQ5T0N5.
PRIDEiQ5T0N5.

PTM databases

iPTMnetiQ5T0N5.
PhosphoSitePlusiQ5T0N5.

Expressioni

Gene expression databases

BgeeiENSG00000137942.
CleanExiHS_FNBP1L.
ExpressionAtlasiQ5T0N5. baseline and differential.
GenevisibleiQ5T0N5. HS.

Organism-specific databases

HPAiHPA065273.

Interactioni

Subunit structurei

Homodimerizes, the dimers can polymerize end-to-end to form filamentous structures (By similarity). Interacts with GTP-bound CDC42 (PubMed:15260990). Interacts with DAAM1, DIAPH1, DIAPH2, DNM1, DNM2 and WASL/N-WASP (PubMed:16326391, PubMed:16630611, PubMed:16418535). Interacts with ATG3 (PubMed:19342671). Interacts (via SH3 domain) with ABI1, WASF2, CDC42 and WIPF1 (PubMed:19798448).By similarity6 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
DAAM1Q9Y4D12EBI-714058,EBI-2817289

GO - Molecular functioni

  • cadherin binding involved in cell-cell adhesion Source: BHF-UCL

Protein-protein interaction databases

BioGridi120222. 24 interactors.
IntActiQ5T0N5. 13 interactors.
MINTiMINT-1424364.
STRINGi9606.ENSP00000271234.

Structurei

3D structure databases

ProteinModelPortaliQ5T0N5.
SMRiQ5T0N5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 263F-BARPROSITE-ProRule annotationAdd BLAST263
Repeati409 – 484REMAdd BLAST76
Domaini538 – 599SH3PROSITE-ProRule annotationAdd BLAST62

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni245 – 535Interaction with CDC42Add BLAST291
Regioni522 – 605Interaction with DNM11 PublicationAdd BLAST84
Regioni541 – 605Interaction with DAAM1, DIAPH1 and DIAPH21 PublicationAdd BLAST65
Regioni541 – 597Interaction with DNM2 and WASL1 PublicationAdd BLAST57

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili66 – 258By similarityAdd BLAST193
Coiled coili392 – 484By similarityAdd BLAST93

Domaini

The F-BAR domain binds the phospholipid membrane with its concave surface. The end-to-end polymerization of dimers of these domains provides a curved surface that fits best membranes with around 600 A diameter, and may drive tubulation (By similarity).By similarity

Sequence similaritiesi

Belongs to the FNBP1 family.Curated
Contains 1 F-BAR domain.PROSITE-ProRule annotation
Contains 1 REM (Hr1) repeat.Curated
Contains 1 SH3 domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, SH3 domain

Phylogenomic databases

eggNOGiENOG410INNW. Eukaryota.
ENOG410XS31. LUCA.
GeneTreeiENSGT00510000046403.
HOGENOMiHOG000231767.
HOVERGENiHBG002489.
InParanoidiQ5T0N5.
KOiK20121.
PhylomeDBiQ5T0N5.

Family and domain databases

InterProiIPR031160. F_BAR.
IPR001060. FCH_dom.
IPR030116. FNBP1L.
IPR001452. SH3_domain.
[Graphical view]
PANTHERiPTHR10663:SF154. PTHR10663:SF154. 1 hit.
PfamiPF00611. FCH. 1 hit.
PF00018. SH3_1. 1 hit.
[Graphical view]
SMARTiSM00055. FCH. 1 hit.
SM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 1 hit.
PROSITEiPS51741. F_BAR. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]

Sequences (5)i

Sequence statusi: Complete.

This entry describes 5 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q5T0N5-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSWGTELWDQ FDSLDKHTQW GIDFLERYAK FVKERIEIEQ NYAKQLRNLV
60 70 80 90 100
KKYCPKRSSK DEEPRFTSCV AFFNILNELN DYAGQREVVA EEMAHRVYGE
110 120 130 140 150
LMRYAHDLKT ERKMHLQEGR KAQQYLDMCW KQMDNSKKKF ERECREAEKA
160 170 180 190 200
QQSYERLDND TNATKADVEK AKQQLNLRTH MADENKNEYA AQLQNFNGEQ
210 220 230 240 250
HKHFYVVIPQ IYKQLQEMDE RRTIKLSECY RGFADSERKV IPIISKCLEG
260 270 280 290 300
MILAAKSVDE RRDSQMVVDS FKSGFEPPGD FPFEDYSQHI YRTISDGTIS
310 320 330 340 350
ASKQESGKMD AKTTVGKAKG KLWLFGKKPK PQSPPLTPTS LFTSSTPNGS
360 370 380 390 400
QFLTFSIEPV HYCMNEIKTG KPRIPSFRSL KRGWSVKMGP ALEDFSHLPP
410 420 430 440 450
EQRRKKLQQR IDELNRELQK ESDQKDALNK MKDVYEKNPQ MGDPGSLQPK
460 470 480 490 500
LAETMNNIDR LRMEIHKNEA WLSEVEGKTG GRGDRRHSSD INHLVTQGRE
510 520 530 540 550
SPEGSYTDDA NQEVRGPPQQ HGHHNEFDDE FEDDDPLPAI GHCKAIYPFD
560 570 580 590 600
GHNEGTLAMK EGEVLYIIEE DKGDGWTRAR RQNGEEGYVP TSYIDVTLEK

NSKGS
Note: Gene prediction based on EST data.
Length:605
Mass (Da):70,065
Last modified:July 9, 2014 - v3
Checksum:i1D9237185C3006ED
GO
Isoform 2 (identifier: Q5T0N5-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     384-388: Missing.

Note: Gene prediction based on EST data.
Show »
Length:600
Mass (Da):69,434
Checksum:i1C2DDFB3A3A5F0B3
GO
Isoform 3 (identifier: Q5T0N5-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     331-388: Missing.

Show »
Length:547
Mass (Da):63,572
Checksum:i416101498FF99567
GO
Isoform 4 (identifier: Q5T0N5-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     331-388: Missing.
     605-605: S → AVTYI

Note: Gene prediction based on EST data.
Show »
Length:551
Mass (Da):64,033
Checksum:iB14EB59CE9010149
GO
Isoform 5 (identifier: Q5T0N5-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     384-388: Missing.
     605-605: S → AVTYI

Note: Gene prediction based on EST data. No experimental confirmation available.
Show »
Length:604
Mass (Da):69,894
Checksum:i8C72A845744DDFB3
GO

Sequence cautioni

The sequence BAA91051 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_021709331 – 388Missing in isoform 3 and isoform 4. 3 PublicationsAdd BLAST58
Alternative sequenceiVSP_021710384 – 388Missing in isoform 2 and isoform 5. Curated5
Alternative sequenceiVSP_021711605S → AVTYI in isoform 4 and isoform 5. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY514449 mRNA. Translation: AAR98814.1.
AC095034 Genomic DNA. No translation available.
AL109613 Genomic DNA. No translation available.
AL512651 Genomic DNA. No translation available.
BC062477 mRNA. Translation: AAH62477.1.
BC074891 mRNA. Translation: AAH74891.1.
BC074892 mRNA. Translation: AAH74892.1.
AK000282 mRNA. Translation: BAA91051.1. Different initiation.
CCDSiCCDS53343.1. [Q5T0N5-1]
CCDS53344.1. [Q5T0N5-4]
CCDS60192.1. [Q5T0N5-3]
RefSeqiNP_001020119.1. NM_001024948.2. [Q5T0N5-4]
NP_001157945.1. NM_001164473.2. [Q5T0N5-1]
NP_060207.2. NM_017737.4. [Q5T0N5-3]
UniGeneiHs.134060.

Genome annotation databases

EnsembliENST00000260506; ENSP00000260506; ENSG00000137942. [Q5T0N5-4]
ENST00000271234; ENSP00000271234; ENSG00000137942. [Q5T0N5-1]
ENST00000370253; ENSP00000359275; ENSG00000137942. [Q5T0N5-3]
GeneIDi54874.
KEGGihsa:54874.
UCSCiuc001dpv.4. human. [Q5T0N5-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY514449 mRNA. Translation: AAR98814.1.
AC095034 Genomic DNA. No translation available.
AL109613 Genomic DNA. No translation available.
AL512651 Genomic DNA. No translation available.
BC062477 mRNA. Translation: AAH62477.1.
BC074891 mRNA. Translation: AAH74891.1.
BC074892 mRNA. Translation: AAH74892.1.
AK000282 mRNA. Translation: BAA91051.1. Different initiation.
CCDSiCCDS53343.1. [Q5T0N5-1]
CCDS53344.1. [Q5T0N5-4]
CCDS60192.1. [Q5T0N5-3]
RefSeqiNP_001020119.1. NM_001024948.2. [Q5T0N5-4]
NP_001157945.1. NM_001164473.2. [Q5T0N5-1]
NP_060207.2. NM_017737.4. [Q5T0N5-3]
UniGeneiHs.134060.

3D structure databases

ProteinModelPortaliQ5T0N5.
SMRiQ5T0N5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi120222. 24 interactors.
IntActiQ5T0N5. 13 interactors.
MINTiMINT-1424364.
STRINGi9606.ENSP00000271234.

PTM databases

iPTMnetiQ5T0N5.
PhosphoSitePlusiQ5T0N5.

Polymorphism and mutation databases

BioMutaiFNBP1L.
DMDMi118572313.

Proteomic databases

EPDiQ5T0N5.
MaxQBiQ5T0N5.
PaxDbiQ5T0N5.
PeptideAtlasiQ5T0N5.
PRIDEiQ5T0N5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000260506; ENSP00000260506; ENSG00000137942. [Q5T0N5-4]
ENST00000271234; ENSP00000271234; ENSG00000137942. [Q5T0N5-1]
ENST00000370253; ENSP00000359275; ENSG00000137942. [Q5T0N5-3]
GeneIDi54874.
KEGGihsa:54874.
UCSCiuc001dpv.4. human. [Q5T0N5-1]

Organism-specific databases

CTDi54874.
DisGeNETi54874.
GeneCardsiFNBP1L.
HGNCiHGNC:20851. FNBP1L.
HPAiHPA065273.
MIMi608848. gene.
neXtProtiNX_Q5T0N5.
OpenTargetsiENSG00000137942.
PharmGKBiPA128394675.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410INNW. Eukaryota.
ENOG410XS31. LUCA.
GeneTreeiENSGT00510000046403.
HOGENOMiHOG000231767.
HOVERGENiHBG002489.
InParanoidiQ5T0N5.
KOiK20121.
PhylomeDBiQ5T0N5.

Enzyme and pathway databases

ReactomeiR-HSA-8856828. Clathrin-mediated endocytosis.

Miscellaneous databases

ChiTaRSiFNBP1L. human.
GeneWikiiFNBP1L.
GenomeRNAii54874.
PROiQ5T0N5.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000137942.
CleanExiHS_FNBP1L.
ExpressionAtlasiQ5T0N5. baseline and differential.
GenevisibleiQ5T0N5. HS.

Family and domain databases

InterProiIPR031160. F_BAR.
IPR001060. FCH_dom.
IPR030116. FNBP1L.
IPR001452. SH3_domain.
[Graphical view]
PANTHERiPTHR10663:SF154. PTHR10663:SF154. 1 hit.
PfamiPF00611. FCH. 1 hit.
PF00018. SH3_1. 1 hit.
[Graphical view]
SMARTiSM00055. FCH. 1 hit.
SM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 1 hit.
PROSITEiPS51741. F_BAR. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiFBP1L_HUMAN
AccessioniPrimary (citable) accession number: Q5T0N5
Secondary accession number(s): J3QSS4
, Q5T0N6, Q6B097, Q6P653, Q6R4Q4, Q9NXG1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 28, 2006
Last sequence update: July 9, 2014
Last modified: November 2, 2016
This is version 122 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.