Q5T013 (HYI_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Putative hydroxypyruvate isomerase
EC=5.3.1.22

Alternative name(s):
Endothelial cell apoptosis protein E-CE1

Gene names

Name:	HYI
ORF Names:	HT036, SB156

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	277 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the reversible isomerization between hydroxypyruvate and 2-hydroxy-3-oxopropanoate (also termed tartronate semialdehyde) By similarity.
Catalytic activity	Hydroxypyruvate = 2-hydroxy-3-oxopropanoate.
Induction	Up-regulated by exposure to 7,8-Dihydroxy-9,10-epoxy-7,8,9,10-tetrahydrobenzo[a]pyrene (BPDE) after which is found in the culture medium of amniotic epithelial cells. Ref.7
Sequence similarities	Belongs to the hyi family.
Sequence caution	The sequence AAH06140.1 differs from that shown. Reason: Frameshift at position 271. The sequence AAH19041.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended. The sequence AAV84474.1 differs from that shown. Reason: Frameshift at positions 215, 228, 232 and 238.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing Polymorphism
Molecular function	Isomerase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Molecular_function	hydroxypyruvate isomerase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q5T013-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q5T013-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-73: Missing.
     248-277: Missing.

Isoform 3 (identifier: Q5T013-3)

The sequence of this isoform differs from the canonical sequence as follows:
     248-277: Missing.

Isoform 4 (identifier: Q5T013-4)

The sequence of this isoform differs from the canonical sequence as follows:
     1-73: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 277	277	Putative hydroxypyruvate isomerase		PRO_0000289241
Natural variations
	Alternative sequence	1 – 73	73	Missing in isoform 2 and isoform 4.		VSP_025986
	Alternative sequence	248 – 277	30	Missing in isoform 2 and isoform 3.		VSP_025987
	Natural variant	239	1	D → N. Ref.6 Corresponds to variant rs17850049 [ dbSNP | Ensembl ].		VAR_032611
Experimental info
	Sequence conflict	18	1	S → P in AAV84474. Ref.1
	Sequence conflict	18	1	S → P in AAK67642. Ref.3
	Sequence conflict	18	1	S → P in AAH19041. Ref.5
	Sequence conflict	224	1	S → KP in AAV84474. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified May 29, 2007. Version 2. Checksum: 79D5072A8717B4F7	FASTA	277	30,406
	10 20 30 40 50 60 MAPLRFSANL SWLFPELSGL PARVRAAGSS GFEAVEVAWP YAETPEALAR AAREAGLRLV 70 80 90 100 110 120 LINTPPGDQE KGEMGLGAVP GRQAAFREGL EQAVRYAKAL GCPRIHLMAG RVPQGADRIA 130 140 150 160 170 180 VKAEMEAVFL ENLRHAAGVL AQEDLVGLLE PINTRITDPQ YFLDTPQQAA AILQKVGRPN 190 200 210 220 230 240 LQLQMDIFHW QIMDGNLTGN IREFLPIVGH VQVAQVPGRG EPSSPGELNF PYLFQLLEDE 250 260 270 GYKGFVGCEY QPRGDTVEGL SWLRSYWDRR GHPEAGQ
	Isoform 2 [UniParc]. Checksum: D04A820535AB1705	FASTA	174	19,084
	10 20 30 40 50 60 MGLGAVPGRQ AAFREGLEQA VRYAKALGCP RIHLMAGRVP QGADRIAVKA EMEAVFLENL 70 80 90 100 110 120 RHAAGVLAQE DLVGLLEPIN TRITDPQYFL DTPQQAAAIL QKVGRPNLQL QMDIFHWQIM 130 140 150 160 170 DGNLTGNIRE FLPIVGHVQV AQVPGRGEPS SPGELNFPYL FQLLEDEGYK GFVG
	Isoform 3 [UniParc]. Checksum: DA0485BAB2BB5724	FASTA	247	26,874
	10 20 30 40 50 60 MAPLRFSANL SWLFPELSGL PARVRAAGSS GFEAVEVAWP YAETPEALAR AAREAGLRLV 70 80 90 100 110 120 LINTPPGDQE KGEMGLGAVP GRQAAFREGL EQAVRYAKAL GCPRIHLMAG RVPQGADRIA 130 140 150 160 170 180 VKAEMEAVFL ENLRHAAGVL AQEDLVGLLE PINTRITDPQ YFLDTPQQAA AILQKVGRPN 190 200 210 220 230 240 LQLQMDIFHW QIMDGNLTGN IREFLPIVGH VQVAQVPGRG EPSSPGELNF PYLFQLLEDE GYKGFVG
	Isoform 4 [UniParc]. Checksum: 80BF040C3520EBD7	FASTA	204	22,616
	10 20 30 40 50 60 MGLGAVPGRQ AAFREGLEQA VRYAKALGCP RIHLMAGRVP QGADRIAVKA EMEAVFLENL 70 80 90 100 110 120 RHAAGVLAQE DLVGLLEPIN TRITDPQYFL DTPQQAAAIL QKVGRPNLQL QMDIFHWQIM 130 140 150 160 170 180 DGNLTGNIRE FLPIVGHVQV AQVPGRGEPS SPGELNFPYL FQLLEDEGYK GFVGCEYQPR 190 200 GDTVEGLSWL RSYWDRRGHP EAGQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Study of a new protein that interacts with endostatin." Zhang B.L., Fan B.X., Liu Y.N. Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3). Tissue: Liver.
[2]	Xu X., Yang Y., Gao G., Xiao H., Chen Z., Han Z. Submitted (JUL-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 4). Tissue: Hypothalamus.
[3]	Li N., Zhang M., Wan T., Zhang W., Cao X. Submitted (MAY-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
[4]	"The DNA sequence and biological annotation of human chromosome 1." Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R. Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 41-247 (ISOFORM 1), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 2-247 (ISOFORM 3), VARIANT ASN-239. Tissue: Kidney and Ovary.
[7]	"Low concentration of anti-7,8-dihydroxy-9,10-epoxy-7,8,9,10-tetrahydrobenzo[a]pyrene induces alterations of extracellular protein profile of exposed epithelial cells." Liu H., Shen J., Feng L., Yu Y. Proteomics 9:4259-4264(2009) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY, INDUCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY775560 mRNA. Translation: AAV84474.1. Frameshift. AF284750 mRNA. Translation: AAG59852.1. AF284751 mRNA. Translation: AAG59853.1. AY037165 mRNA. Translation: AAK67642.1. AL583862 Genomic DNA. Translation: CAI14890.1. AL583862 Genomic DNA. Translation: CAI14893.1. CH471059 Genomic DNA. Translation: EAX07090.1. CH471059 Genomic DNA. Translation: EAX07091.1. CH471059 Genomic DNA. Translation: EAX07093.1. CH471059 Genomic DNA. Translation: EAX07094.1. BC006140 mRNA. Translation: AAH06140.1. Sequence problems. BC019041 mRNA. Translation: AAH19041.1. Different initiation.
IPI	IPI00382748. IPI00465271. IPI00847938. IPI00848045.
RefSeq	NP_001177809.1. NM_001190880.2. NP_001230455.1. NM_001243526.1. NP_112484.3. NM_031207.5.
UniGene	Hs.709864.
3D structure databases
HSSP	HSSP built from PDB template 1K77 based on UniProtKB Q46891.
ProteinModelPortal	Q5T013.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q5T013. 1 interaction.
STRING	9606.ENSP00000361507.
PTM databases
PhosphoSite	Q5T013.
Polymorphism databases
DMDM	152125829.
Proteomic databases
PaxDb	Q5T013.
PRIDE	Q5T013.
Protocols and materials databases
DNASU	81888.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000372425; ENSP00000361502; ENSG00000178922. ENST00000372432; ENSP00000361509; ENSG00000178922. ENST00000583037; ENSP00000461969; ENSG00000178922.
GeneID	81888.
KEGG	hsa:81888.
UCSC	uc001cjm.3. human.
Organism-specific databases
CTD	81888.
GeneCards	GC01M043916.
HGNC	HGNC:26948. HYI.
HPA	HPA040092.
neXtProt	NX_Q5T013.
PharmGKB	PA142671668.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG3622.
HOVERGEN	HBG056845.
InParanoid	Q5T013.
KO	K01816.
OMA	EPINKYA.
Gene expression databases
ArrayExpress	Q5T013.
Bgee	Q5T013.
CleanEx	HS_HYI.
Genevestigator	Q5T013.
Family and domain databases
Gene3D	3.20.20.150. 1 hit.
InterPro	IPR026040. HyI-like. IPR013022. Xyl_isomerase-like_TIM-brl. [Graphical view]
Pfam	PF01261. AP_endonuc_2. 1 hit. [Graphical view]
PIRSF	PIRSF006241. HyI. 1 hit.
SUPFAM	SSF51658. Xyl_isomerase-like_TIM-brl. 1 hit.
ProtoNet	Search...
Other
GenomeRNAi	81888.
NextBio	72232.

Entry information

Entry name

HYI_HUMAN

Accession

Primary (citable) accession number: Q5T013
Secondary accession number(s): D3DPX4 , D3DPX5, Q5Q9A2, Q7Z778, Q96S83, Q9BZR3, Q9BZR4

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 29, 2007
Last sequence update:	May 29, 2007
Last modified:	May 1, 2013
This is version 72 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.