Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Putative hydroxypyruvate isomerase

Gene

HYI

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the reversible isomerization between hydroxypyruvate and 2-hydroxy-3-oxopropanoate (also termed tartronate semialdehyde).By similarity

Catalytic activityi

Hydroxypyruvate = 2-hydroxy-3-oxopropanoate.

GO - Molecular functioni

  1. hydroxypyruvate isomerase activity Source: UniProtKB-EC
Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Names & Taxonomyi

Protein namesi
Recommended name:
Putative hydroxypyruvate isomerase (EC:5.3.1.22)
Alternative name(s):
Endothelial cell apoptosis protein E-CE1
Gene namesi
Name:HYI
ORF Names:HT036, SB156
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 1

Organism-specific databases

HGNCiHGNC:26948. HYI.

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA142671668.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 277277Putative hydroxypyruvate isomerasePRO_0000289241Add
BLAST

Proteomic databases

MaxQBiQ5T013.
PaxDbiQ5T013.
PRIDEiQ5T013.

PTM databases

PhosphoSiteiQ5T013.

Expressioni

Inductioni

Up-regulated by exposure to 7,8-Dihydroxy-9,10-epoxy-7,8,9,10-tetrahydrobenzo[a]pyrene (BPDE) after which is found in the culture medium of amniotic epithelial cells.1 Publication

Gene expression databases

BgeeiQ5T013.
CleanExiHS_HYI.
ExpressionAtlasiQ5T013. baseline and differential.
GenevestigatoriQ5T013.

Organism-specific databases

HPAiHPA040092.

Interactioni

Protein-protein interaction databases

BioGridi123621. 4 interactions.
IntActiQ5T013. 2 interactions.
STRINGi9606.ENSP00000361507.

Structurei

3D structure databases

ProteinModelPortaliQ5T013.
SMRiQ5T013. Positions 5-268.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the hyi family.Curated

Phylogenomic databases

eggNOGiCOG3622.
GeneTreeiENSGT00390000005462.
HOVERGENiHBG056845.
InParanoidiQ5T013.
KOiK01816.
PhylomeDBiQ5T013.
TreeFamiTF312858.

Family and domain databases

Gene3Di3.20.20.150. 1 hit.
InterProiIPR026040. HyI-like.
IPR013022. Xyl_isomerase-like_TIM-brl.
[Graphical view]
PfamiPF01261. AP_endonuc_2. 1 hit.
[Graphical view]
PIRSFiPIRSF006241. HyI. 1 hit.
SUPFAMiSSF51658. SSF51658. 1 hit.

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q5T013-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAPLRFSANL SWLFPELSGL PARVRAAGSS GFEAVEVAWP YAETPEALAR
60 70 80 90 100
AAREAGLRLV LINTPPGDQE KGEMGLGAVP GRQAAFREGL EQAVRYAKAL
110 120 130 140 150
GCPRIHLMAG RVPQGADRIA VKAEMEAVFL ENLRHAAGVL AQEDLVGLLE
160 170 180 190 200
PINTRITDPQ YFLDTPQQAA AILQKVGRPN LQLQMDIFHW QIMDGNLTGN
210 220 230 240 250
IREFLPIVGH VQVAQVPGRG EPSSPGELNF PYLFQLLEDE GYKGFVGCEY
260 270
QPRGDTVEGL SWLRSYWDRR GHPEAGQ
Length:277
Mass (Da):30,406
Last modified:May 29, 2007 - v2
Checksum:i79D5072A8717B4F7
GO
Isoform 2 (identifier: Q5T013-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-73: Missing.
     248-277: Missing.

Show »
Length:174
Mass (Da):19,084
Checksum:iD04A820535AB1705
GO
Isoform 3 (identifier: Q5T013-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     248-277: Missing.

Show »
Length:247
Mass (Da):26,874
Checksum:iDA0485BAB2BB5724
GO
Isoform 4 (identifier: Q5T013-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-73: Missing.

Show »
Length:204
Mass (Da):22,616
Checksum:i80BF040C3520EBD7
GO

Sequence cautioni

The sequence AAH06140.1 differs from that shown. Reason: Frameshift at position 271. Curated
The sequence AAH19041.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence AAV84474.1 differs from that shown. Reason: Frameshift at positions 215, 228, 232 and 238. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti18 – 181S → P in AAV84474 (Ref. 1) Curated
Sequence conflicti18 – 181S → P in AAK67642 (Ref. 3) Curated
Sequence conflicti18 – 181S → P in AAH19041 (Ref. 5) Curated
Sequence conflicti224 – 2241S → KP in AAV84474 (Ref. 1) Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti239 – 2391D → N.1 Publication
Corresponds to variant rs17850049 [ dbSNP | Ensembl ].
VAR_032611

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 7373Missing in isoform 2 and isoform 4. 1 PublicationVSP_025986Add
BLAST
Alternative sequencei248 – 27730Missing in isoform 2 and isoform 3. 4 PublicationsVSP_025987Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY775560 mRNA. Translation: AAV84474.1. Frameshift.
AF284750 mRNA. Translation: AAG59852.1.
AF284751 mRNA. Translation: AAG59853.1.
AY037165 mRNA. Translation: AAK67642.1.
AL583862 Genomic DNA. Translation: CAI14890.1.
AL583862 Genomic DNA. Translation: CAI14893.1.
CH471059 Genomic DNA. Translation: EAX07090.1.
CH471059 Genomic DNA. Translation: EAX07091.1.
CH471059 Genomic DNA. Translation: EAX07093.1.
CH471059 Genomic DNA. Translation: EAX07094.1.
BC006140 mRNA. Translation: AAH06140.1. Sequence problems.
BC019041 mRNA. Translation: AAH19041.1. Different initiation.
CCDSiCCDS488.2. [Q5T013-3]
CCDS53309.1. [Q5T013-1]
RefSeqiNP_001177809.1. NM_001190880.2. [Q5T013-1]
NP_001230455.1. NM_001243526.1.
NP_112484.3. NM_031207.5. [Q5T013-3]
UniGeneiHs.709864.

Genome annotation databases

EnsembliENST00000372425; ENSP00000361502; ENSG00000178922. [Q5T013-1]
ENST00000372432; ENSP00000361509; ENSG00000178922. [Q5T013-3]
GeneIDi81888.
KEGGihsa:81888.
UCSCiuc001cjm.3. human. [Q5T013-1]

Polymorphism databases

DMDMi152125829.

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY775560 mRNA. Translation: AAV84474.1. Frameshift.
AF284750 mRNA. Translation: AAG59852.1.
AF284751 mRNA. Translation: AAG59853.1.
AY037165 mRNA. Translation: AAK67642.1.
AL583862 Genomic DNA. Translation: CAI14890.1.
AL583862 Genomic DNA. Translation: CAI14893.1.
CH471059 Genomic DNA. Translation: EAX07090.1.
CH471059 Genomic DNA. Translation: EAX07091.1.
CH471059 Genomic DNA. Translation: EAX07093.1.
CH471059 Genomic DNA. Translation: EAX07094.1.
BC006140 mRNA. Translation: AAH06140.1. Sequence problems.
BC019041 mRNA. Translation: AAH19041.1. Different initiation.
CCDSiCCDS488.2. [Q5T013-3]
CCDS53309.1. [Q5T013-1]
RefSeqiNP_001177809.1. NM_001190880.2. [Q5T013-1]
NP_001230455.1. NM_001243526.1.
NP_112484.3. NM_031207.5. [Q5T013-3]
UniGeneiHs.709864.

3D structure databases

ProteinModelPortaliQ5T013.
SMRiQ5T013. Positions 5-268.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi123621. 4 interactions.
IntActiQ5T013. 2 interactions.
STRINGi9606.ENSP00000361507.

PTM databases

PhosphoSiteiQ5T013.

Polymorphism databases

DMDMi152125829.

Proteomic databases

MaxQBiQ5T013.
PaxDbiQ5T013.
PRIDEiQ5T013.

Protocols and materials databases

DNASUi81888.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000372425; ENSP00000361502; ENSG00000178922. [Q5T013-1]
ENST00000372432; ENSP00000361509; ENSG00000178922. [Q5T013-3]
GeneIDi81888.
KEGGihsa:81888.
UCSCiuc001cjm.3. human. [Q5T013-1]

Organism-specific databases

CTDi81888.
GeneCardsiGC01M043916.
HGNCiHGNC:26948. HYI.
HPAiHPA040092.
neXtProtiNX_Q5T013.
PharmGKBiPA142671668.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiCOG3622.
GeneTreeiENSGT00390000005462.
HOVERGENiHBG056845.
InParanoidiQ5T013.
KOiK01816.
PhylomeDBiQ5T013.
TreeFamiTF312858.

Miscellaneous databases

GenomeRNAii81888.
NextBioi72232.
PROiQ5T013.

Gene expression databases

BgeeiQ5T013.
CleanExiHS_HYI.
ExpressionAtlasiQ5T013. baseline and differential.
GenevestigatoriQ5T013.

Family and domain databases

Gene3Di3.20.20.150. 1 hit.
InterProiIPR026040. HyI-like.
IPR013022. Xyl_isomerase-like_TIM-brl.
[Graphical view]
PfamiPF01261. AP_endonuc_2. 1 hit.
[Graphical view]
PIRSFiPIRSF006241. HyI. 1 hit.
SUPFAMiSSF51658. SSF51658. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Study of a new protein that interacts with endostatin."
    Zhang B.L., Fan B.X., Liu Y.N.
    Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3).
    Tissue: Liver.
  2. Xu X., Yang Y., Gao G., Xiao H., Chen Z., Han Z.
    Submitted (JUL-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 4).
    Tissue: Hypothalamus.
  3. Li N., Zhang M., Wan T., Zhang W., Cao X.
    Submitted (MAY-2001) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
  4. "The DNA sequence and biological annotation of human chromosome 1."
    Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K.
    , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.
    Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 41-247 (ISOFORM 1), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 2-247 (ISOFORM 3), VARIANT ASN-239.
    Tissue: Kidney and Ovary.
  7. "Low concentration of anti-7,8-dihydroxy-9,10-epoxy-7,8,9,10-tetrahydrobenzo[a]pyrene induces alterations of extracellular protein profile of exposed epithelial cells."
    Liu H., Shen J., Feng L., Yu Y.
    Proteomics 9:4259-4264(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY, INDUCTION.

Entry informationi

Entry nameiHYI_HUMAN
AccessioniPrimary (citable) accession number: Q5T013
Secondary accession number(s): D3DPX4
, D3DPX5, Q5Q9A2, Q7Z778, Q96S83, Q9BZR3, Q9BZR4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: May 29, 2007
Last modified: February 4, 2015
This is version 85 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.