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Protein

Polynucleotide 5'-hydroxyl-kinase NOL9

Gene

NOL9

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Polynucleotide 5'-kinase involved in rRNA processing. The kinase activity is required for the processing of the 32S precursor into 5.8S and 28S rRNAs, more specifically for the generation of the major 5.8S(S) form. In vitro, has both DNA and RNA 5'-kinase activities. Probably binds RNA.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi306 – 313ATPCurated8

GO - Molecular functioni

  • ATP binding Source: UniProtKB
  • polynucleotide 5'-hydroxyl-kinase activity Source: UniProtKB
  • RNA binding Source: UniProtKB-KW

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

rRNA processing

Keywords - Ligandi

ATP-binding, Nucleotide-binding, RNA-binding

Enzyme and pathway databases

ReactomeiR-HSA-6791226. Major pathway of rRNA processing in the nucleolus and cytosol.

Names & Taxonomyi

Protein namesi
Recommended name:
Polynucleotide 5'-hydroxyl-kinase NOL9 (EC:2.7.1.-)
Alternative name(s):
Nucleolar protein 9
Gene namesi
Name:NOL9
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

HGNCiHGNC:26265. NOL9.

Subcellular locationi

GO - Cellular componenti

  • membrane Source: UniProtKB
  • nucleolus Source: UniProtKB
  • nucleoplasm Source: Reactome
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi312K → A: Abolishes kinase activity and rRNA processing. 1 Publication1
Mutagenesisi313S → A: Abolishes kinase activity and rRNA processing. 1 Publication1

Organism-specific databases

DisGeNETi79707.
OpenTargetsiENSG00000162408.
PharmGKBiPA142671256.

Polymorphism and mutation databases

BioMutaiNOL9.
DMDMi73921242.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00000969392 – 702Polynucleotide 5'-hydroxyl-kinase NOL9Add BLAST701

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineCombined sources1
Modified residuei487PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ5SY16.
MaxQBiQ5SY16.
PaxDbiQ5SY16.
PeptideAtlasiQ5SY16.
PRIDEiQ5SY16.

2D gel databases

SWISS-2DPAGEQ5SY16.

PTM databases

iPTMnetiQ5SY16.
PhosphoSitePlusiQ5SY16.
SwissPalmiQ5SY16.

Expressioni

Gene expression databases

BgeeiENSG00000162408.
CleanExiHS_NOL9.
GenevisibleiQ5SY16. HS.

Interactioni

Subunit structurei

Interacts with PELP1, WDR18 and SENP3.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
AHNAKQ09666-23EBI-1055462,EBI-10245106
RELQ048643EBI-1055462,EBI-307352
TCF4P158843EBI-1055462,EBI-533224

Protein-protein interaction databases

BioGridi122825. 38 interactors.
IntActiQ5SY16. 15 interactors.
MINTiMINT-3068215.
STRINGi9606.ENSP00000366934.

Structurei

3D structure databases

ProteinModelPortaliQ5SY16.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the Clp1 family. NOL9/GRC3 subfamily.Curated

Phylogenomic databases

eggNOGiKOG2750. Eukaryota.
COG1341. LUCA.
GeneTreeiENSGT00390000012406.
HOGENOMiHOG000113821.
HOVERGENiHBG058105.
KOiK06947.
OMAiFGFTISQ.
OrthoDBiEOG091G0BNE.
PhylomeDBiQ5SY16.
TreeFamiTF313802.

Family and domain databases

InterProiIPR032319. CLP1_P.
[Graphical view]
PfamiPF16575. CLP1_P. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q5SY16-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADSGLLLKR GSCRSTWLRV RKARPQLILS RRPRRRLGSL RWCGRRRLRW
60 70 80 90 100
RLLQAQASGV DWREGARQVS RAAAARRPNT ATPSPIPSPT PASEPESEPE
110 120 130 140 150
LESASSCHRP LLIPPVRPVG PGRALLLLPV EQGFTFSGIC RVTCLYGQVQ
160 170 180 190 200
VFGFTISQGQ PAQDIFSVYT HSCLSIHALH YSQPEKSKKE LKREARNLLK
210 220 230 240 250
SHLNLDDRRW SMQNFSPQCS IVLLEHLKTA TVNFITSYPG SSYIFVQESP
260 270 280 290 300
TPQIKPEYLA LRSVGIRREK KRKGLQLTES TLSALEELVN VSCEEVDGCP
310 320 330 340 350
VILVCGSQDV GKSTFNRYLI NHLLNSLPCV DYLECDLGQT EFTPPGCISL
360 370 380 390 400
LNITEPVLGP PFTHLRTPQK MVYYGKPSCK NNYENYIDIV KYVFSAYKRE
410 420 430 440 450
SPLIVNTMGW VSDQGLLLLI DLIRLLSPSH VVQFRSDHSK YMPDLTPQYV
460 470 480 490 500
DDMDGLYTKS KTKMRNRRFR LAAFADALEF ADEEKESPVE FTGHKLIGVY
510 520 530 540 550
TDFAFRITPR NRESHNKILR DLSILSYLSQ LQPPMPKPLS PLHSLTPYQV
560 570 580 590 600
PFNAVALRIT HSDVAPTHIL YAVNASWVGL CKIQDDVRGY TNGPILLAQT
610 620 630 640 650
PICDCLGFGI CRGIDMEKRL YHILTPVPPE ELRTVNCLLV GAIAIPHCVL
660 670 680 690 700
KCQRGIEGTV PYVTTDYNFK LPGASEKIGA REPEEAHKEK PYRRPKFCRK

MK
Length:702
Mass (Da):79,323
Last modified:December 21, 2004 - v1
Checksum:i5D42AF5F7F292997
GO

Sequence cautioni

The sequence BAB15611 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_05477710R → W.2 PublicationsCorresponds to variant rs4908923dbSNPEnsembl.1
Natural variantiVAR_05477850W → R.2 PublicationsCorresponds to variant rs6693400dbSNPEnsembl.1
Natural variantiVAR_05477958S → A.2 PublicationsCorresponds to variant rs6693391dbSNPEnsembl.1
Natural variantiVAR_056955420I → V.Corresponds to variant rs17029613dbSNPEnsembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK026976 mRNA. Translation: BAB15611.1. Different initiation.
AK289653 mRNA. Translation: BAF82342.1.
AL591866 Genomic DNA. Translation: CAI16071.1.
BC009257 mRNA. Translation: AAH09257.2.
BC012439 mRNA. Translation: AAH12439.1.
BC063639 mRNA. Translation: AAH63639.1.
BC094836 mRNA. Translation: AAH94836.1.
BC105095 mRNA. Translation: AAI05096.1.
BC110849 mRNA. Translation: AAI10850.1.
BC112278 mRNA. Translation: AAI12279.1.
CCDSiCCDS80.1.
RefSeqiNP_078930.3. NM_024654.4.
XP_011540449.1. XM_011542147.2.
XP_016857827.1. XM_017002338.1.
XP_016857828.1. XM_017002339.1.
UniGeneiHs.59425.

Genome annotation databases

EnsembliENST00000377705; ENSP00000366934; ENSG00000162408.
GeneIDi79707.
KEGGihsa:79707.
UCSCiuc001ans.4. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK026976 mRNA. Translation: BAB15611.1. Different initiation.
AK289653 mRNA. Translation: BAF82342.1.
AL591866 Genomic DNA. Translation: CAI16071.1.
BC009257 mRNA. Translation: AAH09257.2.
BC012439 mRNA. Translation: AAH12439.1.
BC063639 mRNA. Translation: AAH63639.1.
BC094836 mRNA. Translation: AAH94836.1.
BC105095 mRNA. Translation: AAI05096.1.
BC110849 mRNA. Translation: AAI10850.1.
BC112278 mRNA. Translation: AAI12279.1.
CCDSiCCDS80.1.
RefSeqiNP_078930.3. NM_024654.4.
XP_011540449.1. XM_011542147.2.
XP_016857827.1. XM_017002338.1.
XP_016857828.1. XM_017002339.1.
UniGeneiHs.59425.

3D structure databases

ProteinModelPortaliQ5SY16.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi122825. 38 interactors.
IntActiQ5SY16. 15 interactors.
MINTiMINT-3068215.
STRINGi9606.ENSP00000366934.

PTM databases

iPTMnetiQ5SY16.
PhosphoSitePlusiQ5SY16.
SwissPalmiQ5SY16.

Polymorphism and mutation databases

BioMutaiNOL9.
DMDMi73921242.

2D gel databases

SWISS-2DPAGEQ5SY16.

Proteomic databases

EPDiQ5SY16.
MaxQBiQ5SY16.
PaxDbiQ5SY16.
PeptideAtlasiQ5SY16.
PRIDEiQ5SY16.

Protocols and materials databases

DNASUi79707.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000377705; ENSP00000366934; ENSG00000162408.
GeneIDi79707.
KEGGihsa:79707.
UCSCiuc001ans.4. human.

Organism-specific databases

CTDi79707.
DisGeNETi79707.
GeneCardsiNOL9.
HGNCiHGNC:26265. NOL9.
neXtProtiNX_Q5SY16.
OpenTargetsiENSG00000162408.
PharmGKBiPA142671256.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2750. Eukaryota.
COG1341. LUCA.
GeneTreeiENSGT00390000012406.
HOGENOMiHOG000113821.
HOVERGENiHBG058105.
KOiK06947.
OMAiFGFTISQ.
OrthoDBiEOG091G0BNE.
PhylomeDBiQ5SY16.
TreeFamiTF313802.

Enzyme and pathway databases

ReactomeiR-HSA-6791226. Major pathway of rRNA processing in the nucleolus and cytosol.

Miscellaneous databases

ChiTaRSiNOL9. human.
GeneWikiiNOL9.
GenomeRNAii79707.
PROiQ5SY16.

Gene expression databases

BgeeiENSG00000162408.
CleanExiHS_NOL9.
GenevisibleiQ5SY16. HS.

Family and domain databases

InterProiIPR032319. CLP1_P.
[Graphical view]
PfamiPF16575. CLP1_P. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNOL9_HUMAN
AccessioniPrimary (citable) accession number: Q5SY16
Secondary accession number(s): Q2NL84
, Q4VBY3, Q6P472, Q7L4D6, Q96EE0, Q9H5L4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 30, 2005
Last sequence update: December 21, 2004
Last modified: November 30, 2016
This is version 121 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.