Q5SXJ3 (FANCJ_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Fanconi anemia group J protein homolog
Short name=Protein FACJ
EC=3.6.4.13

Alternative name(s):
ATP-dependent RNA helicase BRIP1
BRCA1-associated C-terminal helicase 1
BRCA1-interacting protein C-terminal helicase 1
Short name=BRCA1-interacting protein 1

Gene names

Name:	Brip1
Synonyms:	Bach1, Fancj

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	1174 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	DNA-dependent ATPase and 5' to 3' DNA helicase required for the maintenance of chromosomal stability. Acts late in the 'Fanconi anemia' pathway, after FANCD2 ubiquitination. Involved in the repair of DNA double-strand breaks by homologous recombination in a manner that depends on its association with BRCA1 By similarity.
Catalytic activity	ATP + H2O = ADP + phosphate.
Cofactor	Binds 1 4Fe-4S cluster By similarity.
Subunit structure	Binds directly to the BRCT domains of BRCA1 By similarity.
Subcellular location	Nucleus By similarity.
Sequence similarities	Belongs to the DEAD box helicase family. DEAH subfamily. Contains 1 helicase ATP-binding domain.

Ontologies

Keywords
Biological process	DNA damage DNA repair
Cellular component	Nucleus
Ligand	4Fe-4S ATP-binding Iron Iron-sulfur Metal-binding Nucleotide-binding
Molecular function	Helicase Hydrolase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	DNA repair Inferred from electronic annotation. Source: UniProtKB-KW regulation of transcription from RNA polymerase II promoter Inferred from electronic annotation. Source: Compara
Cellular_component	cytoplasm Inferred from electronic annotation. Source: Compara nuclear membrane Inferred from electronic annotation. Source: Compara
Molecular_function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ATP-dependent DNA helicase activity Inferred from electronic annotation. Source: InterPro DNA binding Inferred from electronic annotation. Source: InterPro metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1174	1174	Fanconi anemia group J protein homolog		PRO_0000055174
Regions
	Domain	11 – 445	435	Helicase ATP-binding
	Nucleotide binding	185 – 192	8	ATP By similarity
	Region	888 – 1063	176	Interaction with BRCA1 By similarity
	Motif	158 – 175	18	Nuclear localization signal Potential
	Motif	393 – 396	4	DEAH box
Sites
	Metal binding	286	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	301	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	313	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	353	1	Iron-sulfur (4Fe-4S) By similarity
Amino acid modifications
	Modified residue	929	1	Phosphoserine By similarity
	Modified residue	932	1	Phosphoserine By similarity
	Modified residue	994	1	Phosphoserine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5SXJ3 [UniParc]. Last modified December 21, 2004. Version 1. Checksum: 54B244232C4E10B1	FASTA	1,174	131,360
	10 20 30 40 50 60 MSSVLSDYTI GGVKIHFPCR AYPAQLAMMN SIVRGLNSSQ HCLLESPTGS GKSLALLCSA 70 80 90 100 110 120 LAWQQSLSEK PVDEGLNKKP EAPPSCSCAC HSKNFTYSDT NLDTSPHFNS PSKPSSGRNG 130 140 150 160 170 180 VSTPCQDSPE KNTLAAKLSA KKQASIHRDE DDDFQVEKKR IRPLETTQQI RKRHCLEKDV 190 200 210 220 230 240 HHVDARLASE KRVKPESPIG KSFSDRKDSF QNVDGLCSRC CCSAKQGNNQ EPANTVKKDH 250 260 270 280 290 300 GGQCKRPKIY FGTRTHKQIA QITRELRKTA YSGVPMTILS SRDHSCVHPE VVGNFNRKEK 310 320 330 340 350 360 CMELLDGKHG KSCYFYHGVH KISNQQTLQH LQGMSRAWDI EELVSLGRKL KACPYYTARE 370 380 390 400 410 420 LIEDADIVFC PYNYLLDSQI RETMDIKLKG QVVILDEAHN IEDCARESAS YSVTEVQLRF 430 440 450 460 470 480 ARDELDSLIN GNIRKKSHEP LRDVCYNLIN WLETNSKHLV ERGYESSCKI WSGNEMLLNL 490 500 510 520 530 540 YRMGITTATF PVLQRHLSAV LQKEEKVTPI HGKEEAIQIP IISASTQVVL KGLFMVLDYL 550 560 570 580 590 600 FRENSRFADD YKVAIQQTYS WTNQIAIFDK TGVLAVPKNK KHSRQKIGVN ALNFWCLNPA 610 620 630 640 650 660 VAFSDINDKV RTIVLTSGTL SPLKSFSSEL GVTFSIQLEA NHVISNSQVW VGTVGSGPKG 670 680 690 700 710 720 RNLCATFQHT ETFEFQDEVG MLLLSVCQTV SQGILCFLPS YKLLEKLRER WIFTGLWHSL 730 740 750 760 770 780 ESVKTVIAEP QGGEKTDFDE LLQVYYDAIK FKGEKDGALL IAVCRGKVSE GLDFSDDNAR 790 800 810 820 830 840 AVITVGIPFP NVKDLQVELK RQYNDHHSKS RGLLPGRQWY EIQAYRALNQ ALGRCIRHKN 850 860 870 880 890 900 DWGALILVDD RFNNNPNRYI SGLSKWVRQQ IQHHSSFASA LESLTEFSRR HQKVTNRSKK 910 920 930 940 950 960 DEKCTKDNEP TLEVACLEDS TFTSVSESSH QSPENSTEEA EVCVQELQCP QVATKSPSVA 970 980 990 1000 1010 1020 SHGVSRRKKS DPGLRGESLQ TMKTEKNEIS RSSSPTFGKQ TEPVNWPIFN SLRRHFNSKV 1030 1040 1050 1060 1070 1080 KNCTPVLKSS KNRAPGSSTF NKTALPLTGN CVPSNETADT SLGPCLQSEV IISPVKIEAT 1090 1100 1110 1120 1130 1140 PATNYSKQVF CCEKDLLPDT ELSPGTEEAK CPSSNKAAET EVDDDSECFT PELFDPVDTN 1150 1160 1170 EENGELVETD RSSHSSDCFS AEELFETATG FGQK

References

[1]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6J.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6. Tissue: Brain.
[3]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-577 AND 754-1174. Strain: C57BL/6J. Tissue: Embryo and Eye.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL592465, AL592065, AL645690 Genomic DNA. Translation: CAI25368.1. AL645690, AL592065, AL592465 Genomic DNA. Translation: CAI24235.1. AL592065, AL592465, AL645690 Genomic DNA. Translation: CAI25868.1. BC094252 mRNA. Translation: AAH94252.1. AK051878 mRNA. Translation: BAC34798.2. AK080771 mRNA. Translation: BAC38016.1.
IPI	IPI00319607.
RefSeq	NP_840094.1. NM_178309.2.
UniGene	Mm.186143.
3D structure databases
ProteinModelPortal	Q5SXJ3.
SMR	Q5SXJ3. Positions 18-63, 246-444.
ModBase	Search...
Protein-protein interaction databases
STRING	10090.ENSMUSP00000043108.
PTM databases
PhosphoSite	Q5SXJ3.
Proteomic databases
PRIDE	Q5SXJ3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000044423; ENSMUSP00000043108; ENSMUSG00000034329.
GeneID	237911.
KEGG	mmu:237911.
UCSC	uc007ksf.2. mouse.
Organism-specific databases
CTD	83990.
MGI	MGI:2442836. Brip1.
Phylogenomic databases
eggNOG	COG1199.
GeneTree	ENSGT00530000063199.
HOGENOM	HOG000068083.
HOVERGEN	HBG081519.
InParanoid	Q5SXJ3.
KO	K15362.
OMA	GVHKISD.
OrthoDB	EOG4J6RQ8.
Gene expression databases
ArrayExpress	Q5SXJ3.
Bgee	Q5SXJ3.
CleanEx	MM_BACH1. MM_BRIP1.
Genevestigator	Q5SXJ3.
GermOnline	ENSMUSG00000034329. Mus musculus.
Family and domain databases
InterPro	IPR006555. ATP-dep_Helicase_C. IPR010614. DEAD_2. IPR013020. DNA_helicase_DNA-repair_Rad3. IPR014013. Helic_SF1/SF2_ATP-bd_DinG/Rad3. IPR014001. Helicase_ATP-bd. [Graphical view]
Pfam	PF06733. DEAD_2. 1 hit. PF13307. Helicase_C_2. 1 hit. [Graphical view]
SMART	SM00487. DEXDc. 1 hit. SM00491. HELICc2. 1 hit. [Graphical view]
TIGRFAMs	TIGR00604. rad3. 1 hit.
PROSITE	PS00690. DEAH_ATP_HELICASE. False negative. PS51193. HELICASE_ATP_BIND_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	BRIP1. mouse.
NextBio	383570.
SOURCE	Search...

Entry information

Entry name

FANCJ_MOUSE

Accession

Primary (citable) accession number: Q5SXJ3
Secondary accession number(s): Q8BJQ8, Q8BKI6

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 25, 2005
Last sequence update:	December 21, 2004
Last modified:	May 1, 2013
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program