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Protein

Fanconi anemia group J protein homolog

Gene

Brip1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

DNA-dependent ATPase and 5' to 3' DNA helicase required for the maintenance of chromosomal stability. Acts late in the 'Fanconi anemia' pathway, after FANCD2 ubiquitination. Involved in the repair of DNA double-strand breaks by homologous recombination in a manner that depends on its association with BRCA1 (By similarity).By similarity

Catalytic activityi

ATP + H2O = ADP + phosphate.

Cofactori

[4Fe-4S] clusterBy similarityNote: Binds 1 [4Fe-4S] cluster.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi286 – 2861Iron-sulfur (4Fe-4S)By similarity
Metal bindingi301 – 3011Iron-sulfur (4Fe-4S)By similarity
Metal bindingi313 – 3131Iron-sulfur (4Fe-4S)By similarity
Metal bindingi353 – 3531Iron-sulfur (4Fe-4S)By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi185 – 1928ATPPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Biological processi

DNA damage, DNA repair

Keywords - Ligandi

4Fe-4S, ATP-binding, Iron, Iron-sulfur, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-5685938. HDR through Single Strand Annealing (SSA).
R-MMU-5685942. HDR through Homologous Recombination (HRR).
R-MMU-5693554. Resolution of D-loop Structures through Synthesis-Dependent Strand Annealing (SDSA).
R-MMU-5693568. Resolution of D-loop Structures through Holliday Junction Intermediates.
R-MMU-5693579. Homologous DNA Pairing and Strand Exchange.
R-MMU-5693607. Processing of DNA double-strand break ends.
R-MMU-5693616. Presynaptic phase of homologous DNA pairing and strand exchange.
R-MMU-6804756. Regulation of TP53 Activity through Phosphorylation.
R-MMU-69473. G2/M DNA damage checkpoint.

Names & Taxonomyi

Protein namesi
Recommended name:
Fanconi anemia group J protein homolog (EC:3.6.4.13)
Short name:
Protein FACJ
Alternative name(s):
ATP-dependent RNA helicase BRIP1
BRCA1-associated C-terminal helicase 1
BRCA1-interacting protein C-terminal helicase 1
Short name:
BRCA1-interacting protein 1
Gene namesi
Name:Brip1
Synonyms:Bach1, Fancj
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:2442836. Brip1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11741174Fanconi anemia group J protein homologPRO_0000055174Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei929 – 9291PhosphoserineBy similarity
Modified residuei932 – 9321PhosphoserineBy similarity
Modified residuei994 – 9941PhosphoserineBy similarity
Modified residuei1174 – 11741N6-acetyllysineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ5SXJ3.
MaxQBiQ5SXJ3.
PaxDbiQ5SXJ3.
PeptideAtlasiQ5SXJ3.
PRIDEiQ5SXJ3.

PTM databases

iPTMnetiQ5SXJ3.
PhosphoSiteiQ5SXJ3.

Expressioni

Gene expression databases

BgeeiQ5SXJ3.
CleanExiMM_BACH1.
MM_BRIP1.
GenevisibleiQ5SXJ3. MM.

Interactioni

Subunit structurei

Binds directly to the BRCT domains of BRCA1.By similarity

Protein-protein interaction databases

BioGridi231925. 1 interaction.
STRINGi10090.ENSMUSP00000043108.

Structurei

3D structure databases

ProteinModelPortaliQ5SXJ3.
SMRiQ5SXJ3. Positions 246-444.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini11 – 445435Helicase ATP-bindingPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni888 – 1063176Interaction with BRCA1By similarityAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi158 – 17518Nuclear localization signalSequence analysisAdd
BLAST
Motifi393 – 3964DEAH box

Sequence similaritiesi

Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG1132. Eukaryota.
COG1199. LUCA.
GeneTreeiENSGT00530000063199.
HOGENOMiHOG000068083.
HOVERGENiHBG081519.
InParanoidiQ5SXJ3.
KOiK15362.
OMAiTNNDMNQ.
OrthoDBiEOG7PS1GJ.
PhylomeDBiQ5SXJ3.
TreeFamiTF329449.

Family and domain databases

Gene3Di3.40.50.300. 4 hits.
InterProiIPR006555. ATP-dep_Helicase_C.
IPR010614. DEAD_2.
IPR013020. DNA_helicase_DNA-repair_Rad3.
IPR014013. Helic_SF1/SF2_ATP-bd_DinG/Rad3.
IPR006554. Helicase-like_DEXD_c2.
IPR014001. Helicase_ATP-bd.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF06733. DEAD_2. 1 hit.
PF13307. Helicase_C_2. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00488. DEXDc2. 1 hit.
SM00491. HELICc2. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 6 hits.
TIGRFAMsiTIGR00604. rad3. 1 hit.
PROSITEiPS51193. HELICASE_ATP_BIND_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5SXJ3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSVLSDYTI GGVKIHFPCR AYPAQLAMMN SIVRGLNSSQ HCLLESPTGS
60 70 80 90 100
GKSLALLCSA LAWQQSLSEK PVDEGLNKKP EAPPSCSCAC HSKNFTYSDT
110 120 130 140 150
NLDTSPHFNS PSKPSSGRNG VSTPCQDSPE KNTLAAKLSA KKQASIHRDE
160 170 180 190 200
DDDFQVEKKR IRPLETTQQI RKRHCLEKDV HHVDARLASE KRVKPESPIG
210 220 230 240 250
KSFSDRKDSF QNVDGLCSRC CCSAKQGNNQ EPANTVKKDH GGQCKRPKIY
260 270 280 290 300
FGTRTHKQIA QITRELRKTA YSGVPMTILS SRDHSCVHPE VVGNFNRKEK
310 320 330 340 350
CMELLDGKHG KSCYFYHGVH KISNQQTLQH LQGMSRAWDI EELVSLGRKL
360 370 380 390 400
KACPYYTARE LIEDADIVFC PYNYLLDSQI RETMDIKLKG QVVILDEAHN
410 420 430 440 450
IEDCARESAS YSVTEVQLRF ARDELDSLIN GNIRKKSHEP LRDVCYNLIN
460 470 480 490 500
WLETNSKHLV ERGYESSCKI WSGNEMLLNL YRMGITTATF PVLQRHLSAV
510 520 530 540 550
LQKEEKVTPI HGKEEAIQIP IISASTQVVL KGLFMVLDYL FRENSRFADD
560 570 580 590 600
YKVAIQQTYS WTNQIAIFDK TGVLAVPKNK KHSRQKIGVN ALNFWCLNPA
610 620 630 640 650
VAFSDINDKV RTIVLTSGTL SPLKSFSSEL GVTFSIQLEA NHVISNSQVW
660 670 680 690 700
VGTVGSGPKG RNLCATFQHT ETFEFQDEVG MLLLSVCQTV SQGILCFLPS
710 720 730 740 750
YKLLEKLRER WIFTGLWHSL ESVKTVIAEP QGGEKTDFDE LLQVYYDAIK
760 770 780 790 800
FKGEKDGALL IAVCRGKVSE GLDFSDDNAR AVITVGIPFP NVKDLQVELK
810 820 830 840 850
RQYNDHHSKS RGLLPGRQWY EIQAYRALNQ ALGRCIRHKN DWGALILVDD
860 870 880 890 900
RFNNNPNRYI SGLSKWVRQQ IQHHSSFASA LESLTEFSRR HQKVTNRSKK
910 920 930 940 950
DEKCTKDNEP TLEVACLEDS TFTSVSESSH QSPENSTEEA EVCVQELQCP
960 970 980 990 1000
QVATKSPSVA SHGVSRRKKS DPGLRGESLQ TMKTEKNEIS RSSSPTFGKQ
1010 1020 1030 1040 1050
TEPVNWPIFN SLRRHFNSKV KNCTPVLKSS KNRAPGSSTF NKTALPLTGN
1060 1070 1080 1090 1100
CVPSNETADT SLGPCLQSEV IISPVKIEAT PATNYSKQVF CCEKDLLPDT
1110 1120 1130 1140 1150
ELSPGTEEAK CPSSNKAAET EVDDDSECFT PELFDPVDTN EENGELVETD
1160 1170
RSSHSSDCFS AEELFETATG FGQK
Length:1,174
Mass (Da):131,360
Last modified:December 21, 2004 - v1
Checksum:i54B244232C4E10B1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL592465, AL592065, AL645690 Genomic DNA. Translation: CAI25368.1.
AL645690, AL592065, AL592465 Genomic DNA. Translation: CAI24235.1.
AL592065, AL592465, AL645690 Genomic DNA. Translation: CAI25868.1.
BC094252 mRNA. Translation: AAH94252.1.
AK051878 mRNA. Translation: BAC34798.2.
AK080771 mRNA. Translation: BAC38016.1.
CCDSiCCDS25197.1.
RefSeqiNP_840094.1. NM_178309.2.
XP_006533302.1. XM_006533239.2.
UniGeneiMm.186143.

Genome annotation databases

EnsembliENSMUST00000044423; ENSMUSP00000043108; ENSMUSG00000034329.
GeneIDi237911.
KEGGimmu:237911.
UCSCiuc007ksf.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL592465, AL592065, AL645690 Genomic DNA. Translation: CAI25368.1.
AL645690, AL592065, AL592465 Genomic DNA. Translation: CAI24235.1.
AL592065, AL592465, AL645690 Genomic DNA. Translation: CAI25868.1.
BC094252 mRNA. Translation: AAH94252.1.
AK051878 mRNA. Translation: BAC34798.2.
AK080771 mRNA. Translation: BAC38016.1.
CCDSiCCDS25197.1.
RefSeqiNP_840094.1. NM_178309.2.
XP_006533302.1. XM_006533239.2.
UniGeneiMm.186143.

3D structure databases

ProteinModelPortaliQ5SXJ3.
SMRiQ5SXJ3. Positions 246-444.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi231925. 1 interaction.
STRINGi10090.ENSMUSP00000043108.

PTM databases

iPTMnetiQ5SXJ3.
PhosphoSiteiQ5SXJ3.

Proteomic databases

EPDiQ5SXJ3.
MaxQBiQ5SXJ3.
PaxDbiQ5SXJ3.
PeptideAtlasiQ5SXJ3.
PRIDEiQ5SXJ3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000044423; ENSMUSP00000043108; ENSMUSG00000034329.
GeneIDi237911.
KEGGimmu:237911.
UCSCiuc007ksf.2. mouse.

Organism-specific databases

CTDi83990.
MGIiMGI:2442836. Brip1.

Phylogenomic databases

eggNOGiKOG1132. Eukaryota.
COG1199. LUCA.
GeneTreeiENSGT00530000063199.
HOGENOMiHOG000068083.
HOVERGENiHBG081519.
InParanoidiQ5SXJ3.
KOiK15362.
OMAiTNNDMNQ.
OrthoDBiEOG7PS1GJ.
PhylomeDBiQ5SXJ3.
TreeFamiTF329449.

Enzyme and pathway databases

ReactomeiR-MMU-5685938. HDR through Single Strand Annealing (SSA).
R-MMU-5685942. HDR through Homologous Recombination (HRR).
R-MMU-5693554. Resolution of D-loop Structures through Synthesis-Dependent Strand Annealing (SDSA).
R-MMU-5693568. Resolution of D-loop Structures through Holliday Junction Intermediates.
R-MMU-5693579. Homologous DNA Pairing and Strand Exchange.
R-MMU-5693607. Processing of DNA double-strand break ends.
R-MMU-5693616. Presynaptic phase of homologous DNA pairing and strand exchange.
R-MMU-6804756. Regulation of TP53 Activity through Phosphorylation.
R-MMU-69473. G2/M DNA damage checkpoint.

Miscellaneous databases

PROiQ5SXJ3.
SOURCEiSearch...

Gene expression databases

BgeeiQ5SXJ3.
CleanExiMM_BACH1.
MM_BRIP1.
GenevisibleiQ5SXJ3. MM.

Family and domain databases

Gene3Di3.40.50.300. 4 hits.
InterProiIPR006555. ATP-dep_Helicase_C.
IPR010614. DEAD_2.
IPR013020. DNA_helicase_DNA-repair_Rad3.
IPR014013. Helic_SF1/SF2_ATP-bd_DinG/Rad3.
IPR006554. Helicase-like_DEXD_c2.
IPR014001. Helicase_ATP-bd.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF06733. DEAD_2. 1 hit.
PF13307. Helicase_C_2. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00488. DEXDc2. 1 hit.
SM00491. HELICc2. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 6 hits.
TIGRFAMsiTIGR00604. rad3. 1 hit.
PROSITEiPS51193. HELICASE_ATP_BIND_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Brain.
  3. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-577 AND 754-1174.
    Strain: C57BL/6J.
    Tissue: Embryo and Eye.

Entry informationi

Entry nameiFANCJ_MOUSE
AccessioniPrimary (citable) accession number: Q5SXJ3
Secondary accession number(s): Q8BJQ8, Q8BKI6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 25, 2005
Last sequence update: December 21, 2004
Last modified: July 6, 2016
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.