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Protein

Zinc finger protein 496

Gene

Znf496

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

DNA-binding transcription factor that can both act as an activator and a repressor.2 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri405 – 427C2H2-type 1; degeneratePROSITE-ProRule annotationAdd BLAST23
Zinc fingeri433 – 455C2H2-type 2PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri461 – 483C2H2-type 3PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri520 – 543C2H2-type 4PROSITE-ProRule annotationAdd BLAST24
Zinc fingeri551 – 573C2H2-type 5PROSITE-ProRule annotationAdd BLAST23

GO - Molecular functioni

  • DNA binding Source: UniProtKB
  • metal ion binding Source: UniProtKB-KW
  • protein self-association Source: MGI
  • RNA polymerase II transcription factor activity, sequence-specific DNA binding Source: GO_Central

GO - Biological processi

  • negative regulation of transcription by RNA polymerase II Source: MGI
  • positive regulation of transcription, DNA-templated Source: UniProtKB
  • transcription, DNA-templated Source: UniProtKB-KW

Keywordsi

Molecular functionActivator, DNA-binding, Repressor
Biological processTranscription, Transcription regulation
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-212436 Generic Transcription Pathway

Names & Taxonomyi

Protein namesi
Recommended name:
Zinc finger protein 496
Alternative name(s):
NSD1-interacting zinc finger protein 1
Zinc finger protein with KRAB and SCAN domains 17
Gene namesi
Name:Znf496
Synonyms:Nizp11, Zfp496, Zkscan17
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:2679270 Zkscan17

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003919061 – 585Zinc finger protein 496Add BLAST585

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki13Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei182PhosphoserineCombined sources1
Cross-linki494Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ5SXI5
PeptideAtlasiQ5SXI5
PRIDEiQ5SXI5

PTM databases

iPTMnetiQ5SXI5
PhosphoSitePlusiQ5SXI5

Expressioni

Gene expression databases

BgeeiENSMUSG00000020472
GenevisibleiQ5SXI5 MM

Interactioni

Subunit structurei

Interacts (via zinc-fingers) with JARID2. Interacts with NSD1.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Jarid2Q623156EBI-7417351,EBI-493592

GO - Molecular functioni

  • protein self-association Source: MGI

Protein-protein interaction databases

IntActiQ5SXI5, 1 interactor
MINTiQ5SXI5
STRINGi10090.ENSMUSP00000013262

Structurei

Secondary structure

1585
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni408 – 410Combined sources3
Beta strandi413 – 415Combined sources3
Helixi417 – 425Combined sources9
Helixi426 – 429Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2NABNMR-A397-434[»]
ProteinModelPortaliQ5SXI5
SMRiQ5SXI5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini42 – 124SCAN boxPROSITE-ProRule annotationAdd BLAST83
Domaini220 – 294KRABPROSITE-ProRule annotationAdd BLAST75

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi575 – 579Nuclear localization signal5

Domaini

The C2H2-type zinc finger 1, also named C2HR, mediates the interaction with NSD1.

Sequence similaritiesi

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri405 – 427C2H2-type 1; degeneratePROSITE-ProRule annotationAdd BLAST23
Zinc fingeri433 – 455C2H2-type 2PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri461 – 483C2H2-type 3PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri520 – 543C2H2-type 4PROSITE-ProRule annotationAdd BLAST24
Zinc fingeri551 – 573C2H2-type 5PROSITE-ProRule annotationAdd BLAST23

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG1721 Eukaryota
COG5048 LUCA
GeneTreeiENSGT00910000144000
HOGENOMiHOG000234619
HOVERGENiHBG058089
InParanoidiQ5SXI5
KOiK09229
OMAiGRPWQWL
OrthoDBiEOG091G06AC
PhylomeDBiQ5SXI5
TreeFamiTF350829

Family and domain databases

CDDicd07765 KRAB_A-box, 1 hit
cd07936 SCAN, 1 hit
Gene3Di1.10.4020.10, 1 hit
InterProiView protein in InterPro
IPR001909 KRAB
IPR036051 KRAB_dom_sf
IPR003309 SCAN_dom
IPR038269 SCAN_sf
IPR027767 Zfp496
IPR036236 Znf_C2H2_sf
IPR013087 Znf_C2H2_type
PANTHERiPTHR45016:SF2 PTHR45016:SF2, 1 hit
PfamiView protein in Pfam
PF01352 KRAB, 1 hit
PF02023 SCAN, 1 hit
SMARTiView protein in SMART
SM00431 SCAN, 1 hit
SM00355 ZnF_C2H2, 5 hits
SUPFAMiSSF109640 SSF109640, 1 hit
SSF57667 SSF57667, 3 hits
PROSITEiView protein in PROSITE
PS50805 KRAB, 1 hit
PS50804 SCAN_BOX, 1 hit
PS00028 ZINC_FINGER_C2H2_1, 4 hits
PS50157 ZINC_FINGER_C2H2_2, 5 hits

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q5SXI5-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MPTALCPRVL APKESEEPRK MRSPPGENPS PQGEPPSPES SRRLFRRFRY
60 70 80 90 100
QEAAGPREAL QRLWELCRGW LRLERHTKEQ ILELLVLEQF LAILPWEIQS
110 120 130 140 150
WVRAQEPESG EQAVAAVEAL EREPGRPWQW LKHCEDPVVI DDGDGPAAPQ
160 170 180 190 200
DLEQERMSAE SQSYPDAPPG ALVQGTGLLS RSPGQPSEDL VPQDAFVVQE
210 220 230 240 250
QSIRDAQPVA TCQLPPNRVS PFKDMILCFS EEDWSLLDPA QTGFYGEFII
260 270 280 290 300
GEDYAVSMPP NEPPVQPGHS HEEENGLRVT EWTTDLQDKE IPQASCLDLS
310 320 330 340 350
SLQPFQGEER RKWEELQVPE LQPCPQVVLS QSPCPAGGDP PALKSSLDQE
360 370 380 390 400
VTIEIVLSSS GDEDSQHSPY CTEELRSPPE DLHSVPAHQS NASAEGEVQT
410 420 430 440 450
SQKSYVCPNC GKIFRWRVNF IRHLRSRREQ KPHKCSVCGE LFSDSEDLDG
460 470 480 490 500
HLETHEAQKP YRCTACGKSF RLNSHLISHR RIHLQPASQQ PMKKSEEEAL
510 520 530 540 550
ETEGTGASDL LEKSKAKLSF QCGDCEKSFQ RHDHLVRHRR HCHLKDETRP
560 570 580
FQCRYCVKTF RQNYDLLRHE RLHMKRRSKQ ALNSY
Length:585
Mass (Da):66,577
Last modified:December 21, 2004 - v1
Checksum:i9F8780B785EF71FA
GO
Isoform 2 (identifier: Q5SXI5-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-20: Missing.

Show »
Length:565
Mass (Da):64,342
Checksum:i72259489B7A1A978
GO
Isoform 3 (identifier: Q5SXI5-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-156: Missing.

Show »
Length:429
Mass (Da):48,577
Checksum:iEAEF7D6619C7375D
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti499A → T in AAH40205 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0387681 – 156Missing in isoform 3. 1 PublicationAdd BLAST156
Alternative sequenceiVSP_0387691 – 20Missing in isoform 2. 1 PublicationAdd BLAST20

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY302242 mRNA Translation: AAQ73562.1
AK051711 mRNA Translation: BAC34730.1
AK139346 mRNA Translation: BAE23969.1
AK155196 mRNA Translation: BAE33110.1
AK155490 mRNA Translation: BAE33290.1
AK170924 mRNA Translation: BAE42117.1
AK170989 mRNA Translation: BAE42163.1
AK171190 mRNA Translation: BAE42300.1
AL592522 Genomic DNA No translation available.
BC040205 mRNA Translation: AAH40205.1
CCDSiCCDS24770.1 [Q5SXI5-1]
CCDS70197.1 [Q5SXI5-3]
RefSeqiNP_001124001.1, NM_001130529.2 [Q5SXI5-2]
NP_001277943.1, NM_001291014.1 [Q5SXI5-3]
NP_766529.3, NM_172941.4 [Q5SXI5-1]
XP_006533447.1, XM_006533384.3 [Q5SXI5-1]
XP_006533449.1, XM_006533386.3 [Q5SXI5-2]
UniGeneiMm.239793
Mm.488731

Genome annotation databases

EnsembliENSMUST00000013262; ENSMUSP00000013262; ENSMUSG00000020472 [Q5SXI5-1]
ENSMUST00000101150; ENSMUSP00000098709; ENSMUSG00000020472 [Q5SXI5-3]
GeneIDi268417
KEGGimmu:268417
UCSCiuc007jea.2 mouse [Q5SXI5-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiZN496_MOUSE
AccessioniPrimary (citable) accession number: Q5SXI5
Secondary accession number(s): Q3TC40
, Q3UTL1, Q8BKK0, Q8CGF9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 2, 2010
Last sequence update: December 21, 2004
Last modified: May 23, 2018
This is version 136 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

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