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Protein

Zinc finger protein 496

Gene

Znf496

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

DNA-binding transcription factor that can both act as an activator and a repressor.2 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri405 – 42723C2H2-type 1; degeneratePROSITE-ProRule annotationAdd
BLAST
Zinc fingeri433 – 45523C2H2-type 2PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri461 – 48323C2H2-type 3PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri520 – 54324C2H2-type 4PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri551 – 57323C2H2-type 5PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  • DNA binding Source: UniProtKB
  • metal ion binding Source: UniProtKB-KW
  • protein self-association Source: MGI
  • RNA polymerase II transcription factor activity, sequence-specific DNA binding Source: GO_Central

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator, Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Zinc finger protein 496
Alternative name(s):
NSD1-interacting zinc finger protein 1
Zinc finger protein with KRAB and SCAN domains 17
Gene namesi
Name:Znf496
Synonyms:Nizp11, Zfp496, Zkscan17
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:2679270. Zkscan17.

Subcellular locationi

GO - Cellular componenti

  • nuclear body Source: MGI
  • nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 585585Zinc finger protein 496PRO_0000391906Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei182 – 1821PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ5SXI5.
PaxDbiQ5SXI5.
PeptideAtlasiQ5SXI5.
PRIDEiQ5SXI5.

PTM databases

iPTMnetiQ5SXI5.
PhosphoSiteiQ5SXI5.

Expressioni

Gene expression databases

BgeeiQ5SXI5.
GenevisibleiQ5SXI5. MM.

Interactioni

Subunit structurei

Interacts (via zinc-fingers) with JARID2. Interacts with NSD1.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Jarid2Q623156EBI-7417351,EBI-493592

GO - Molecular functioni

  • protein self-association Source: MGI

Protein-protein interaction databases

IntActiQ5SXI5. 1 interaction.
MINTiMINT-1177482.
STRINGi10090.ENSMUSP00000013262.

Structurei

Secondary structure

1
585
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Turni408 – 4103Combined sources
Beta strandi413 – 4153Combined sources
Helixi417 – 4259Combined sources
Helixi426 – 4294Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2NABNMR-A397-434[»]
ProteinModelPortaliQ5SXI5.
SMRiQ5SXI5. Positions 36-123, 359-573.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini42 – 12483SCAN boxPROSITE-ProRule annotationAdd
BLAST
Domaini220 – 29475KRABPROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi575 – 5795Nuclear localization signal

Domaini

The C2H2-type zinc finger 1, also named C2HR, mediates the interaction with NSD1.

Sequence similaritiesi

Contains 5 C2H2-type zinc fingers.PROSITE-ProRule annotation
Contains 1 KRAB domain.PROSITE-ProRule annotation
Contains 1 SCAN box domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri405 – 42723C2H2-type 1; degeneratePROSITE-ProRule annotationAdd
BLAST
Zinc fingeri433 – 45523C2H2-type 2PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri461 – 48323C2H2-type 3PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri520 – 54324C2H2-type 4PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri551 – 57323C2H2-type 5PROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG1721. Eukaryota.
COG5048. LUCA.
GeneTreeiENSGT00840000129746.
HOGENOMiHOG000234619.
HOVERGENiHBG058089.
InParanoidiQ5SXI5.
KOiK09229.
OMAiRIHLQPD.
OrthoDBiEOG71CFKS.
PhylomeDBiQ5SXI5.
TreeFamiTF350829.

Family and domain databases

Gene3Di3.30.160.60. 4 hits.
InterProiIPR027775. C2H2_Znf_fam.
IPR001909. KRAB.
IPR008916. Retrov_capsid_C.
IPR003309. SCAN_dom.
IPR027767. Zfp496.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PANTHERiPTHR10032. PTHR10032. 2 hits.
PTHR10032:SF212. PTHR10032:SF212. 2 hits.
PfamiPF01352. KRAB. 1 hit.
PF02023. SCAN. 1 hit.
[Graphical view]
SMARTiSM00431. SCAN. 1 hit.
SM00355. ZnF_C2H2. 5 hits.
[Graphical view]
SUPFAMiSSF109640. SSF109640. 1 hit.
SSF47353. SSF47353. 1 hit.
PROSITEiPS50805. KRAB. 1 hit.
PS50804. SCAN_BOX. 1 hit.
PS00028. ZINC_FINGER_C2H2_1. 4 hits.
PS50157. ZINC_FINGER_C2H2_2. 5 hits.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q5SXI5-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MPTALCPRVL APKESEEPRK MRSPPGENPS PQGEPPSPES SRRLFRRFRY
60 70 80 90 100
QEAAGPREAL QRLWELCRGW LRLERHTKEQ ILELLVLEQF LAILPWEIQS
110 120 130 140 150
WVRAQEPESG EQAVAAVEAL EREPGRPWQW LKHCEDPVVI DDGDGPAAPQ
160 170 180 190 200
DLEQERMSAE SQSYPDAPPG ALVQGTGLLS RSPGQPSEDL VPQDAFVVQE
210 220 230 240 250
QSIRDAQPVA TCQLPPNRVS PFKDMILCFS EEDWSLLDPA QTGFYGEFII
260 270 280 290 300
GEDYAVSMPP NEPPVQPGHS HEEENGLRVT EWTTDLQDKE IPQASCLDLS
310 320 330 340 350
SLQPFQGEER RKWEELQVPE LQPCPQVVLS QSPCPAGGDP PALKSSLDQE
360 370 380 390 400
VTIEIVLSSS GDEDSQHSPY CTEELRSPPE DLHSVPAHQS NASAEGEVQT
410 420 430 440 450
SQKSYVCPNC GKIFRWRVNF IRHLRSRREQ KPHKCSVCGE LFSDSEDLDG
460 470 480 490 500
HLETHEAQKP YRCTACGKSF RLNSHLISHR RIHLQPASQQ PMKKSEEEAL
510 520 530 540 550
ETEGTGASDL LEKSKAKLSF QCGDCEKSFQ RHDHLVRHRR HCHLKDETRP
560 570 580
FQCRYCVKTF RQNYDLLRHE RLHMKRRSKQ ALNSY
Length:585
Mass (Da):66,577
Last modified:December 21, 2004 - v1
Checksum:i9F8780B785EF71FA
GO
Isoform 2 (identifier: Q5SXI5-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-20: Missing.

Show »
Length:565
Mass (Da):64,342
Checksum:i72259489B7A1A978
GO
Isoform 3 (identifier: Q5SXI5-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-156: Missing.

Show »
Length:429
Mass (Da):48,577
Checksum:iEAEF7D6619C7375D
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti499 – 4991A → T in AAH40205 (PubMed:15489334).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 156156Missing in isoform 3. 1 PublicationVSP_038768Add
BLAST
Alternative sequencei1 – 2020Missing in isoform 2. 1 PublicationVSP_038769Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY302242 mRNA. Translation: AAQ73562.1.
AK051711 mRNA. Translation: BAC34730.1.
AK139346 mRNA. Translation: BAE23969.1.
AK155196 mRNA. Translation: BAE33110.1.
AK155490 mRNA. Translation: BAE33290.1.
AK170924 mRNA. Translation: BAE42117.1.
AK170989 mRNA. Translation: BAE42163.1.
AK171190 mRNA. Translation: BAE42300.1.
AL592522 Genomic DNA. Translation: CAI24553.1.
AL592522 Genomic DNA. Translation: CAI24554.1.
BC040205 mRNA. Translation: AAH40205.1.
CCDSiCCDS24770.1. [Q5SXI5-1]
CCDS70197.1. [Q5SXI5-3]
RefSeqiNP_001124001.1. NM_001130529.2. [Q5SXI5-2]
NP_001277943.1. NM_001291014.1. [Q5SXI5-3]
NP_766529.3. NM_172941.4. [Q5SXI5-1]
XP_006533447.1. XM_006533384.2. [Q5SXI5-1]
XP_006533449.1. XM_006533386.2. [Q5SXI5-2]
UniGeneiMm.239793.
Mm.488731.

Genome annotation databases

EnsembliENSMUST00000013262; ENSMUSP00000013262; ENSMUSG00000020472. [Q5SXI5-1]
ENSMUST00000101150; ENSMUSP00000098709; ENSMUSG00000020472. [Q5SXI5-3]
GeneIDi268417.
KEGGimmu:268417.
UCSCiuc007jea.2. mouse. [Q5SXI5-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY302242 mRNA. Translation: AAQ73562.1.
AK051711 mRNA. Translation: BAC34730.1.
AK139346 mRNA. Translation: BAE23969.1.
AK155196 mRNA. Translation: BAE33110.1.
AK155490 mRNA. Translation: BAE33290.1.
AK170924 mRNA. Translation: BAE42117.1.
AK170989 mRNA. Translation: BAE42163.1.
AK171190 mRNA. Translation: BAE42300.1.
AL592522 Genomic DNA. Translation: CAI24553.1.
AL592522 Genomic DNA. Translation: CAI24554.1.
BC040205 mRNA. Translation: AAH40205.1.
CCDSiCCDS24770.1. [Q5SXI5-1]
CCDS70197.1. [Q5SXI5-3]
RefSeqiNP_001124001.1. NM_001130529.2. [Q5SXI5-2]
NP_001277943.1. NM_001291014.1. [Q5SXI5-3]
NP_766529.3. NM_172941.4. [Q5SXI5-1]
XP_006533447.1. XM_006533384.2. [Q5SXI5-1]
XP_006533449.1. XM_006533386.2. [Q5SXI5-2]
UniGeneiMm.239793.
Mm.488731.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2NABNMR-A397-434[»]
ProteinModelPortaliQ5SXI5.
SMRiQ5SXI5. Positions 36-123, 359-573.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ5SXI5. 1 interaction.
MINTiMINT-1177482.
STRINGi10090.ENSMUSP00000013262.

PTM databases

iPTMnetiQ5SXI5.
PhosphoSiteiQ5SXI5.

Proteomic databases

MaxQBiQ5SXI5.
PaxDbiQ5SXI5.
PeptideAtlasiQ5SXI5.
PRIDEiQ5SXI5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000013262; ENSMUSP00000013262; ENSMUSG00000020472. [Q5SXI5-1]
ENSMUST00000101150; ENSMUSP00000098709; ENSMUSG00000020472. [Q5SXI5-3]
GeneIDi268417.
KEGGimmu:268417.
UCSCiuc007jea.2. mouse. [Q5SXI5-1]

Organism-specific databases

CTDi268417.
MGIiMGI:2679270. Zkscan17.

Phylogenomic databases

eggNOGiKOG1721. Eukaryota.
COG5048. LUCA.
GeneTreeiENSGT00840000129746.
HOGENOMiHOG000234619.
HOVERGENiHBG058089.
InParanoidiQ5SXI5.
KOiK09229.
OMAiRIHLQPD.
OrthoDBiEOG71CFKS.
PhylomeDBiQ5SXI5.
TreeFamiTF350829.

Miscellaneous databases

PROiQ5SXI5.
SOURCEiSearch...

Gene expression databases

BgeeiQ5SXI5.
GenevisibleiQ5SXI5. MM.

Family and domain databases

Gene3Di3.30.160.60. 4 hits.
InterProiIPR027775. C2H2_Znf_fam.
IPR001909. KRAB.
IPR008916. Retrov_capsid_C.
IPR003309. SCAN_dom.
IPR027767. Zfp496.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PANTHERiPTHR10032. PTHR10032. 2 hits.
PTHR10032:SF212. PTHR10032:SF212. 2 hits.
PfamiPF01352. KRAB. 1 hit.
PF02023. SCAN. 1 hit.
[Graphical view]
SMARTiSM00431. SCAN. 1 hit.
SM00355. ZnF_C2H2. 5 hits.
[Graphical view]
SUPFAMiSSF109640. SSF109640. 1 hit.
SSF47353. SSF47353. 1 hit.
PROSITEiPS50805. KRAB. 1 hit.
PS50804. SCAN_BOX. 1 hit.
PS00028. ZINC_FINGER_C2H2_1. 4 hits.
PS50157. ZINC_FINGER_C2H2_2. 5 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Nizp1, a novel multitype zinc finger protein that interacts with the NSD1 histone lysine methyltransferase through a unique C2HR motif."
    Nielsen A.L., Jorgensen P., Lerouge T., Cervino M., Chambon P., Losson R.
    Mol. Cell. Biol. 24:5184-5196(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH NSD1.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 2 AND 3).
    Strain: C57BL/6J and NOD.
    Tissue: Brain cortex and Spinal ganglion.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: FVB/N.
    Tissue: Mammary tumor.
  5. "Characterization of zinc finger protein 496 that interacts with Jumonji/JARID2."
    Mysliwiec M.R., Kim T.G., Lee Y.
    FEBS Lett. 581:2633-2640(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH JARID2.
  6. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-182, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Kidney.

Entry informationi

Entry nameiZN496_MOUSE
AccessioniPrimary (citable) accession number: Q5SXI5
Secondary accession number(s): Q3TC40
, Q3UTL1, Q8BKK0, Q8CGF9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 2, 2010
Last sequence update: December 21, 2004
Last modified: July 6, 2016
This is version 121 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.