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Protein

Myosin-1

Gene

Myh1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Muscle contraction.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi179 – 186ATPSequence analysis8

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Motor protein, Muscle protein, Myosin

Keywords - Ligandi

Actin-binding, ATP-binding, Calmodulin-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Myosin-1
Alternative name(s):
Myosin heavy chain 1
Myosin heavy chain 2x
Short name:
MyHC-2x
Myosin heavy chain, skeletal muscle, adult 1
Gene namesi
Name:Myh1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:1339711. Myh1.

Subcellular locationi

GO - Cellular componenti

  • A band Source: MGI
  • cytoplasmic ribonucleoprotein granule Source: MGI
  • intercalated disc Source: MGI
  • muscle myosin complex Source: MGI
  • myosin filament Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Thick filament

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001233921 – 1942Myosin-1Add BLAST1942

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei36PhosphoserineBy similarity1
Modified residuei64PhosphothreonineBy similarity1
Modified residuei69PhosphothreonineBy similarity1
Modified residuei130N6,N6,N6-trimethyllysineSequence analysis1
Modified residuei389PhosphotyrosineBy similarity1
Modified residuei419PhosphothreonineBy similarity1
Modified residuei424PhosphotyrosineBy similarity1
Modified residuei625PhosphoserineBy similarity1
Modified residuei760Pros-methylhistidineBy similarity1
Modified residuei1095PhosphoserineBy similarity1
Modified residuei1099PhosphoserineBy similarity1
Modified residuei1165PhosphoserineBy similarity1
Modified residuei1240PhosphoserineBy similarity1
Modified residuei1246PhosphoserineBy similarity1
Modified residuei1258PhosphothreonineBy similarity1
Modified residuei1264PhosphoserineBy similarity1
Modified residuei1268PhosphothreonineBy similarity1
Modified residuei1289PhosphothreonineBy similarity1
Modified residuei1291PhosphoserineBy similarity1
Modified residuei1295PhosphoserineBy similarity1
Modified residuei1306PhosphoserineBy similarity1
Modified residuei1309PhosphoserineBy similarity1
Modified residuei1467PhosphotyrosineBy similarity1
Modified residuei1470PhosphothreonineBy similarity1
Modified residuei1477PhosphoserineBy similarity1
Modified residuei1495PhosphotyrosineBy similarity1
Modified residuei1498PhosphoserineBy similarity1
Modified residuei1504PhosphothreonineBy similarity1
Modified residuei1517PhosphoserineBy similarity1
Modified residuei1520PhosphothreonineBy similarity1
Modified residuei1545PhosphoserineBy similarity1
Modified residuei1557PhosphoserineBy similarity1
Modified residuei1577PhosphoserineBy similarity1
Modified residuei1603PhosphoserineBy similarity1
Modified residuei1606PhosphoserineBy similarity1
Modified residuei1717PhosphoserineBy similarity1
Modified residuei1729PhosphoserineBy similarity1
Modified residuei1733PhosphothreonineBy similarity1
Modified residuei1739PhosphothreonineBy similarity1
Modified residuei1742PhosphoserineBy similarity1
Cross-linki1816Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity

Keywords - PTMi

Isopeptide bond, Methylation, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ5SX40.
MaxQBiQ5SX40.
PaxDbiQ5SX40.
PRIDEiQ5SX40.

PTM databases

iPTMnetiQ5SX40.
PhosphoSitePlusiQ5SX40.

Expressioni

Gene expression databases

BgeeiENSMUSG00000056328.
ExpressionAtlasiQ5SX40. baseline and differential.
GenevisibleiQ5SX40. MM.

Interactioni

Subunit structurei

Muscle myosin is a hexameric protein that consists of 2 heavy chain subunits (MHC), 2 alkali light chain subunits (MLC) and 2 regulatory light chain subunits (MLC-2).

Protein-protein interaction databases

BioGridi201643. 3 interactors.
IntActiQ5SX40. 2 interactors.
MINTiMINT-4102740.
STRINGi10090.ENSMUSP00000018637.

Structurei

3D structure databases

ProteinModelPortaliQ5SX40.
SMRiQ5SX40.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini86 – 785Myosin motorAdd BLAST700
Domaini788 – 817IQPROSITE-ProRule annotationAdd BLAST30

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni662 – 684Actin-bindingBy similarityAdd BLAST23
Regioni764 – 778Actin-bindingBy similarityAdd BLAST15

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili846 – 1942Sequence analysisAdd BLAST1097

Domaini

The rodlike tail sequence is highly repetitive, showing cycles of a 28-residue repeat pattern composed of 4 heptapeptides, characteristic for alpha-helical coiled coils.
Limited proteolysis of myosin heavy chain produces 1 light meromyosin (LMM) and 1 heavy meromyosin (HMM). HMM can be further cleaved into 2 globular subfragments (S1) and 1 rod-shaped subfragment (S2).Curated

Sequence similaritiesi

Contains 1 IQ domain.PROSITE-ProRule annotation
Contains 1 myosin motor domain.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG0161. Eukaryota.
COG5022. LUCA.
GeneTreeiENSGT00760000118919.
HOGENOMiHOG000173959.
HOVERGENiHBG004704.
InParanoidiQ5SX40.
KOiK10352.
OMAiIMDVEND.
OrthoDBiEOG091G07UM.
PhylomeDBiQ5SX40.
TreeFamiTF314375.

Family and domain databases

Gene3Di4.10.270.10. 1 hit.
InterProiIPR000048. IQ_motif_EF-hand-BS.
IPR027401. Myosin-like_IQ_dom.
IPR001609. Myosin_head_motor_dom.
IPR004009. Myosin_N.
IPR002928. Myosin_tail.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00063. Myosin_head. 1 hit.
PF02736. Myosin_N. 1 hit.
PF01576. Myosin_tail_1. 1 hit.
[Graphical view]
PRINTSiPR00193. MYOSINHEAVY.
SMARTiSM00015. IQ. 1 hit.
SM00242. MYSc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS50096. IQ. 1 hit.
PS51456. MYOSIN_MOTOR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5SX40-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSDAEMAVF GEAAPYLRKS EKERIEAQNK PFDAKSSVFV VDAKESFVKA
60 70 80 90 100
TVQSREGGKV TAKTEGGTTV TVKDDQVYPM NPPKYDKIED MAMMTHLHEP
110 120 130 140 150
AVLYNLKERY AAWMIYTYSG LFCVTVNPYK WLPVYNAEVV AAYRGKKRQE
160 170 180 190 200
APPHIFSISD NAYQFMLTDR ENQSILITGE SGAGKTVNTK RVIQYFATIA
210 220 230 240 250
VTGEKKKEEA TSGKMQGTLE DQIISANPLL EAFGNAKTVR NDNSSRFGKF
260 270 280 290 300
IRIHFGTTGK LASADIETYL LEKSRVTFQL KAERSYHIFY QIMSNKKPDL
310 320 330 340 350
IEMLLITTNP YDYAFVSQGE ITVPSIDDQE ELMATDSAID ILGFTSDERV
360 370 380 390 400
SIYKLTGAVM HYGNMKFKQK QREEQAEPDG TEVADKAAYL QNLNSADLLK
410 420 430 440 450
ALCYPRVKVG NEYVTKGQTV QQVYNSVGAL AKAVYEKMFL WMVTRINQQL
460 470 480 490 500
DTKQPRQYFI GVLDIAGFEI FDFNSLEQLC INFTNEKLQQ FFNHHMFVLE
510 520 530 540 550
QEEYKKEGIE WEFIDFGMDL AACIELIEKP MGIFSILEEE CMFPKATDTS
560 570 580 590 600
FKNKLYEQHL GKSNNFQKPK PAKGKVEAHF SLVHYAGTVD YNIAGWLDKN
610 620 630 640 650
KDPLNETVVG LYQKSSMKTL AYLFSGAAAA AEAESGGGGG KKGAKKKGSS
660 670 680 690 700
FQTVSALFRE NLNKLMTNLR STHPHFVRCI IPNETKTPGA MEHELVLHQL
710 720 730 740 750
RCNGVLEGIR ICRKGFPSRI LYADFKQRYK VLNASAIPEG QFIDSKKASE
760 770 780 790 800
KLLGSIDIDH TQYKFGHTKV FFKAGLLGLL EEMRDDKLAQ LITRTQAMCR
810 820 830 840 850
GYLARVEYQK MVERRESIFC IQYNVRAFMN VKHWPWMKLY FKIKPLLKSA
860 870 880 890 900
ETEKEMANMK EEFEKAKENL AKAEAKRKEL EEKMVALMQE KNDLQLQVQS
910 920 930 940 950
EADSLADAEE RCDQLIKTKI QLEAKIKEVT ERAEDEEEIN AELTAKKRKL
960 970 980 990 1000
EDECSELKKD IDDLELTLAK VEKEKHATEN KVKNLTEEMA GLDETIAKLT
1010 1020 1030 1040 1050
KEKKALQEAH QQTLDDLQAE EDKVNTLTKA KIKLEQQVDD LEGSLEQEKK
1060 1070 1080 1090 1100
IRMDLERAKR KLEGDLKLAQ ESTMDVENDK QQLDEKLKKK EFEMSNLQSK
1110 1120 1130 1140 1150
IEDEQALGMQ LQKKIKELQA RIEELEEEIE AERASRAKAE KQRSDLSREL
1160 1170 1180 1190 1200
EEISERLEEA GGATSAQIEM NKKREAEFQK MRRDLEEATL QHEATAATLR
1210 1220 1230 1240 1250
KKHADSVAEL GEQIDNLQRV KQKLEKEKSE MKMEIDDLAS NMEVISKSKG
1260 1270 1280 1290 1300
NLEKMCRTLE DQVSELKTKE EEQQRLINEL TAQRGRLQTE SGEYSRQLDE
1310 1320 1330 1340 1350
KDSLVSQLSR GKQAFTQQIE ELKRQLEEEI KAKSALAHAL QSSRHDCDLL
1360 1370 1380 1390 1400
REQYEEEQEA KAELQRAMSK ANSEVAQWRT KYETDAIQRT EELEEAKKKL
1410 1420 1430 1440 1450
AQRLQDAEEH VEAVNAKCAS LEKTKQRLQN EVEDLMIDVE RTNAACAALD
1460 1470 1480 1490 1500
KKQRNFDKIL AEWKQKYEET HAELEASQKE SRSLSTELFK IKNAYEESLD
1510 1520 1530 1540 1550
HLETLKRENK NLQQEISDLT EQIAEGGKRI HELEKIKKQI EQEKSELQAA
1560 1570 1580 1590 1600
LEEAEASLEH EEGKILRIQL ELNQVKSEID RKIAEKDEEI DQLKRNHIRV
1610 1620 1630 1640 1650
VESMQSTLDA EIRSRNDAIR LKKKMEGDLN EMEIQLNHSN RMAAEALRNY
1660 1670 1680 1690 1700
RNTQGILKDT QLHLDDALRG QEDLKEQLAM VERRANLLQA EIEELRATLE
1710 1720 1730 1740 1750
QTERSRKIAE QELLDASERV QLLHTQNTSL INTKKKLETD ISQIQGEMED
1760 1770 1780 1790 1800
IVQEARNAEE KAKKAITDAA MMAEELKKEQ DTSAHLERMK KNLEQTVKDL
1810 1820 1830 1840 1850
QHRLDEAEQL ALKGGKKQIQ KLEARVRELE GEVENEQKRN VEAIKGLRKH
1860 1870 1880 1890 1900
ERRVKELTYQ TEEDRKNVLR LQDLVDKLQS KVKAYKRQAE EAEEQSNVNL
1910 1920 1930 1940
AKFRKIQHEL EEAEERADIA ESQVNKLRVK SREVHTKIIS EE
Length:1,942
Mass (Da):223,342
Last modified:December 21, 2004 - v1
Checksum:i946BBA18567A2FB0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL596129 Genomic DNA. Translation: CAI24987.1.
BC108329 mRNA. Translation: AAI08330.1.
CCDSiCCDS24855.1.
RefSeqiNP_109604.1. NM_030679.1.
XP_017169807.1. XM_017314318.1.
UniGeneiMm.477065.

Genome annotation databases

EnsembliENSMUST00000018637; ENSMUSP00000018637; ENSMUSG00000056328.
ENSMUST00000075734; ENSMUSP00000075147; ENSMUSG00000056328.
ENSMUST00000124516; ENSMUSP00000117569; ENSMUSG00000056328.
GeneIDi17879.
KEGGimmu:17879.
UCSCiuc007jmg.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL596129 Genomic DNA. Translation: CAI24987.1.
BC108329 mRNA. Translation: AAI08330.1.
CCDSiCCDS24855.1.
RefSeqiNP_109604.1. NM_030679.1.
XP_017169807.1. XM_017314318.1.
UniGeneiMm.477065.

3D structure databases

ProteinModelPortaliQ5SX40.
SMRiQ5SX40.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi201643. 3 interactors.
IntActiQ5SX40. 2 interactors.
MINTiMINT-4102740.
STRINGi10090.ENSMUSP00000018637.

PTM databases

iPTMnetiQ5SX40.
PhosphoSitePlusiQ5SX40.

Proteomic databases

EPDiQ5SX40.
MaxQBiQ5SX40.
PaxDbiQ5SX40.
PRIDEiQ5SX40.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000018637; ENSMUSP00000018637; ENSMUSG00000056328.
ENSMUST00000075734; ENSMUSP00000075147; ENSMUSG00000056328.
ENSMUST00000124516; ENSMUSP00000117569; ENSMUSG00000056328.
GeneIDi17879.
KEGGimmu:17879.
UCSCiuc007jmg.1. mouse.

Organism-specific databases

CTDi4619.
MGIiMGI:1339711. Myh1.

Phylogenomic databases

eggNOGiKOG0161. Eukaryota.
COG5022. LUCA.
GeneTreeiENSGT00760000118919.
HOGENOMiHOG000173959.
HOVERGENiHBG004704.
InParanoidiQ5SX40.
KOiK10352.
OMAiIMDVEND.
OrthoDBiEOG091G07UM.
PhylomeDBiQ5SX40.
TreeFamiTF314375.

Miscellaneous databases

PROiQ5SX40.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000056328.
ExpressionAtlasiQ5SX40. baseline and differential.
GenevisibleiQ5SX40. MM.

Family and domain databases

Gene3Di4.10.270.10. 1 hit.
InterProiIPR000048. IQ_motif_EF-hand-BS.
IPR027401. Myosin-like_IQ_dom.
IPR001609. Myosin_head_motor_dom.
IPR004009. Myosin_N.
IPR002928. Myosin_tail.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00063. Myosin_head. 1 hit.
PF02736. Myosin_N. 1 hit.
PF01576. Myosin_tail_1. 1 hit.
[Graphical view]
PRINTSiPR00193. MYOSINHEAVY.
SMARTiSM00015. IQ. 1 hit.
SM00242. MYSc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS50096. IQ. 1 hit.
PS51456. MYOSIN_MOTOR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMYH1_MOUSE
AccessioniPrimary (citable) accession number: Q5SX40
Secondary accession number(s): Q32P18
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 30, 2005
Last sequence update: December 21, 2004
Last modified: November 2, 2016
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Caution

Represents a conventional myosin. This protein should not be confused with the unconventional myosin-1 (MYO1).Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.