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Protein

N-acetylglucosaminyl-phosphatidylinositol de-N-acetylase

Gene

Pigl

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Involved in the second step of GPI biosynthesis. De-N-acetylation of N-acetylglucosaminyl-phosphatidylinositol (By similarity).By similarity

Catalytic activityi

6-(N-acetyl-alpha-D-glucosaminyl)-1-phosphatidyl-1D-myo-inositol + H2O = 6-(alpha-D-glucosaminyl)-1-phosphatidyl-1D-myo-inositol + acetate.

Pathway: glycosylphosphatidylinositol-anchor biosynthesis

This protein is involved in the pathway glycosylphosphatidylinositol-anchor biosynthesis, which is part of Glycolipid biosynthesis.
View all proteins of this organism that are known to be involved in the pathway glycosylphosphatidylinositol-anchor biosynthesis and in Glycolipid biosynthesis.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

GPI-anchor biosynthesis

Enzyme and pathway databases

ReactomeiREACT_336927. Synthesis of glycosylphosphatidylinositol (GPI).
UniPathwayiUPA00196.

Names & Taxonomyi

Protein namesi
Recommended name:
N-acetylglucosaminyl-phosphatidylinositol de-N-acetylase (EC:3.5.1.89)
Alternative name(s):
Phosphatidylinositol-glycan biosynthesis class L protein
Short name:
PIG-L
Gene namesi
Name:Pigl
Synonyms:Gm737
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:2681271. Pigl.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei2 – 2221HelicalSequence AnalysisAdd
BLAST
Topological domaini23 – 252230CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 252252N-acetylglucosaminyl-phosphatidylinositol de-N-acetylasePRO_0000307824Add
BLAST

Proteomic databases

PRIDEiQ5SX19.

Expressioni

Gene expression databases

BgeeiQ5SX19.
GenevisibleiQ5SX19. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000014389.

Structurei

3D structure databases

ProteinModelPortaliQ5SX19.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the PIGL family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG2120.
GeneTreeiENSGT00390000018434.
HOGENOMiHOG000176611.
HOVERGENiHBG019149.
InParanoidiQ5SX19.
KOiK03434.
OMAiHTQDVLF.
OrthoDBiEOG7KWSJF.
PhylomeDBiQ5SX19.
TreeFamiTF315150.

Family and domain databases

Gene3Di3.40.50.10320. 1 hit.
InterProiIPR003737. GlcNAc_PI_deacetylase-related.
IPR024078. LmbE-like_dom.
[Graphical view]
PANTHERiPTHR12993. PTHR12993. 1 hit.
PfamiPF02585. PIG-L. 1 hit.
[Graphical view]
SUPFAMiSSF102588. SSF102588. 1 hit.

Sequencei

Sequence statusi: Complete.

Q5SX19-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MELVGFLCVA VAVLTWGFLR VWNSAERMRS PEQAGLPGAG SRALVVIAHP
60 70 80 90 100
DDEAMFFAPT MLGLARLEQQ VSLLCFSSGN YYNQGEIRKK ELLQSCAVLG
110 120 130 140 150
IPPSRVMIID KRDFPDDPEV QWDTELVAST LLQHIHANGT DLVVTFDAEG
160 170 180 190 200
VSGHSNHIAL YKAVRALHSG GKLPKGCSVL TLQSVNALRK YAFLLDLPWT
210 220 230 240 250
LLSPQDVLFV LTSKEVAQAK KAMSCHRSQL LWFRYLYVLF SRYMRINSLR

FL
Length:252
Mass (Da):28,183
Last modified:December 21, 2004 - v1
Checksum:i5C243350751F3A6F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK158149 mRNA. Translation: BAE34385.1.
BX248411, AL596181, BX470084 Genomic DNA. Translation: CAI24059.1.
AL596181, BX248411, BX470084 Genomic DNA. Translation: CAI24669.1.
BX470084, AL596181, BX248411 Genomic DNA. Translation: CAI24686.1.
CCDSiCCDS24824.1.
RefSeqiNP_001034625.1. NM_001039536.2.
UniGeneiMm.390358.

Genome annotation databases

EnsembliENSMUST00000014389; ENSMUSP00000014389; ENSMUSG00000014245.
GeneIDi327942.
KEGGimmu:327942.
UCSCiuc007jje.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK158149 mRNA. Translation: BAE34385.1.
BX248411, AL596181, BX470084 Genomic DNA. Translation: CAI24059.1.
AL596181, BX248411, BX470084 Genomic DNA. Translation: CAI24669.1.
BX470084, AL596181, BX248411 Genomic DNA. Translation: CAI24686.1.
CCDSiCCDS24824.1.
RefSeqiNP_001034625.1. NM_001039536.2.
UniGeneiMm.390358.

3D structure databases

ProteinModelPortaliQ5SX19.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000014389.

Proteomic databases

PRIDEiQ5SX19.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000014389; ENSMUSP00000014389; ENSMUSG00000014245.
GeneIDi327942.
KEGGimmu:327942.
UCSCiuc007jje.1. mouse.

Organism-specific databases

CTDi9487.
MGIiMGI:2681271. Pigl.

Phylogenomic databases

eggNOGiCOG2120.
GeneTreeiENSGT00390000018434.
HOGENOMiHOG000176611.
HOVERGENiHBG019149.
InParanoidiQ5SX19.
KOiK03434.
OMAiHTQDVLF.
OrthoDBiEOG7KWSJF.
PhylomeDBiQ5SX19.
TreeFamiTF315150.

Enzyme and pathway databases

UniPathwayiUPA00196.
ReactomeiREACT_336927. Synthesis of glycosylphosphatidylinositol (GPI).

Miscellaneous databases

NextBioi398008.
PROiQ5SX19.
SOURCEiSearch...

Gene expression databases

BgeeiQ5SX19.
GenevisibleiQ5SX19. MM.

Family and domain databases

Gene3Di3.40.50.10320. 1 hit.
InterProiIPR003737. GlcNAc_PI_deacetylase-related.
IPR024078. LmbE-like_dom.
[Graphical view]
PANTHERiPTHR12993. PTHR12993. 1 hit.
PfamiPF02585. PIG-L. 1 hit.
[Graphical view]
SUPFAMiSSF102588. SSF102588. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Inner ear.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.

Entry informationi

Entry nameiPIGL_MOUSE
AccessioniPrimary (citable) accession number: Q5SX19
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 23, 2007
Last sequence update: December 21, 2004
Last modified: June 24, 2015
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.