Q5SWZ9 (PLD6_MOUSE) Reviewed, UniProtKB/Swiss-Prot
Last modified
September 21, 2011.
Version 57.
History...
Names·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
Names·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: Mitochondrial cardiolipin hydrolase EC=3.1.4.- Alternative name(s): Choline phosphatase 6 Mitochondrial phospholipase Short name=MitoPLD Phosphatidylcholine-hydrolyzing phospholipase D6 Phospholipase D6 Short name=PLD 6 | ||
| Gene names |
| ||
| Organism | Mus musculus (Mouse) | ||
| Taxonomic identifier | 10090 [NCBI] | ||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus |
Protein attributes
| Sequence length | 221 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at transcript level |
General annotation (Comments)
| Function | Cardiolipin hydrolase present at the mitochondrial outer membrane required both for mitochondrial fusion and piRNA metabolic process. Acts by catalyzing the hydrolysis of cardiolipin (diphosphatidylglycerol) to form phosphatidate (phosphatidic acid or PA) at the mitochondrial outer membrane surface, promoting mitochondrial fusion. The production of phosphatidate also regulates the piRNA metabolic process by promoting recruitment and/or activation of components of the meiotic nuage, also named P granule, a critical step for primary biogenesis of piRNAs. Required during spermatogenesis to repress transposable elements and prevent their mobilization via its role in the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and govern the methylation and subsequent repression of transposons. Ref.4 Ref.5 |
| Subunit structure | Homodimer By similarity. |
| Subcellular location | Mitochondrion outer membrane; Single-pass membrane protein Ref.4. |
| Tissue specificity | Mainly expressed in testis and ovary. Ref.4 |
| Domain | In contrast to other members of the phospholipase D family, contains only one PLD phosphodiesterase domain, suggesting that it has a single half-catalytic and requires homodimerization to form a complete active site By similarity. |
| Disruption phenotype | Male sterility because of meiotic arrest during spermatogenesis due to demethylation and subsequent derepression of transposable elements. Effects are caused by defects in primary piRNA biogenesis: in contrast to wild-type cells neither mitochondria nor associated meiotic nuage (named P granule) are aggregated. Ref.4 Ref.5 |
| Sequence similarities | Belongs to the phospholipase D family. MitoPLD/Zucchini subfamily. Contains 1 PLD phosphodiesterase domain. |
Ontologies
Alternative products
| This entry describes 2 isoforms produced by alternative splicing. [Align] [Select] | ||||||
| Isoform 1 (identifier: Q5SWZ9-1) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. | ||||||
| Isoform 2 (identifier: Q5SWZ9-2) The sequence of this isoform differs from the canonical sequence as follows: 139-147: AGIQVRHDQ → GYRYGTTRT 148-221: Missing. |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 221 | 221 | Mitochondrial cardiolipin hydrolase | PRO_0000325911 | |||||
Regions | |||||||||
| Topological domain | 1 – 4 | 4 | Mitochondrial intermembrane Potential | ||||||
| Transmembrane | 5 – 27 | 23 | Helical; Potential | ||||||
| Topological domain | 28 – 221 | 194 | Cytoplasmic Potential | ||||||
| Domain | 148 – 175 | 28 | PLD phosphodiesterase | ||||||
Sites | |||||||||
| Active site | 153 | 1 | Potential | ||||||
| Active site | 155 | 1 | Potential | ||||||
| Active site | 160 | 1 | Potential | ||||||
Natural variations | |||||||||
| Alternative sequence | 139 – 147 | 9 | AGIQVRHDQ → GYRYGTTRT in isoform 2. | VSP_032474 | |||||
| Alternative sequence | 148 – 221 | 74 | Missing in isoform 2. | VSP_032475 | |||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. Hayashizaki Y.Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Strain: C57BL/6J. Tissue: Egg and Testis. |
| [2] | "Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. Ponting C.P.PLoS Biol. 7:E1000112-E1000112(2009) [PubMed: 19468303] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6J. |
| [3] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-195 (ISOFORM 1). Tissue: Brain. |
| [4] | "MITOPLD is a mitochondrial protein essential for nuage formation and piRNA biogenesis in the mouse germline." Watanabe T., Chuma S., Yamamoto Y., Kuramochi-Miyagawa S., Totoki Y., Toyoda A., Hoki Y., Fujiyama A., Shibata T., Sado T., Noce T., Nakano T., Nakatsuji N., Lin H., Sasaki H. Dev. Cell 20:364-375(2011) [PubMed: 21397847] [Abstract] Cited for: FUNCTION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY, SUBCELLULAR LOCATION. |
| [5] | "piRNA-associated germline nuage formation and spermatogenesis require MitoPLD profusogenic mitochondrial-surface lipid signaling." Huang H., Gao Q., Peng X., Choi S.Y., Sarma K., Ren H., Morris A.J., Frohman M.A. Dev. Cell 20:376-387(2011) [PubMed: 21397848] [Abstract] Cited for: FUNCTION, DISRUPTION PHENOTYPE. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | AK077214 mRNA. Translation: BAC36687.1. AK139586 mRNA. Translation: BAE24077.1. AL596204 Genomic DNA. Translation: CAI24298.1. BC119245 mRNA. Translation: AAI19246.1. BC145052 mRNA. Translation: AAI45053.1. |
| IPI | IPI00225919. IPI00889236. |
| RefSeq | NP_898962.2. NM_183139.2. |
| UniGene | Mm.344651. |
3D structure databases | |
| ProteinModelPortal | Q5SWZ9. |
| SMR | Q5SWZ9. Positions 74-210. |
| ModBase | Search... |
Protein-protein interaction databases | |
| STRING | Q5SWZ9. |
Proteomic databases | |
| PRIDE | Q5SWZ9. |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| Ensembl | ENSMUST00000125307; ENSMUSP00000115503; ENSMUSG00000043648. |
| GeneID | 194908. |
| KEGG | mmu:194908. |
| UCSC | uc007jev.1. mouse. uc011xvp.1. mouse. |
Organism-specific databases | |
| CTD | 201164. |
| MGI | MGI:2687283. Pld6. |
Phylogenomic databases | |
| eggNOG | roNOG08350. |
| GeneTree | ENSGT00390000004368. |
| HOGENOM | HBG714389. |
| HOVERGEN | HBG108267. |
| InParanoid | Q5SWZ9. |
| OMA | PRREVLF. |
| OrthoDB | EOG402WTD. |
Gene expression databases | |
| ArrayExpress | Q5SWZ9. |
| Bgee | Q5SWZ9. |
| Genevestigator | Q5SWZ9. |
Family and domain databases | |
| InterPro | IPR001736. PLipase_D/transphosphatidylase. [Graphical view] |
| SMART | SM00155. PLDc. 1 hit. [Graphical view] |
| PROSITE | PS50035. PLD. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other | |
| NextBio | 371672. |
| SOURCE | Search... |
Entry information
| Entry name | PLD6_MOUSE | ||||||||
| Accession | Primary (citable) accession number: Q5SWZ9 Secondary accession number(s): B7ZN71, Q3UTA3, Q8BVM0 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Chordata Protein Annotation Program | ||||||||
Relevant documents
| MGD cross-references Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot |
| SIMILARITY comments Index of protein domains and families |

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