Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Mediator of RNA polymerase II transcription subunit 13

Gene

Med13

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator, Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-MMU-212436. Generic Transcription Pathway.
R-MMU-381340. Transcriptional regulation of white adipocyte differentiation.
R-MMU-442533. Transcriptional Regulation of Adipocyte Differentiation in 3T3-L1 Pre-adipocytes.

Names & Taxonomyi

Protein namesi
Recommended name:
Mediator of RNA polymerase II transcription subunit 13
Alternative name(s):
Thyroid hormone receptor-associated protein 1
Thyroid hormone receptor-associated protein complex 240 kDa component
Short name:
Trap240
Gene namesi
Name:Med13
Synonyms:Kiaa0593, Thrap1, Trap240
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:3029632. Med13.

Subcellular locationi

GO - Cellular componenti

  • mediator complex Source: MGI
  • membrane Source: MGI
  • nucleoplasm Source: MGI
  • nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 21712171Mediator of RNA polymerase II transcription subunit 13PRO_0000314240Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei500 – 5001PhosphoserineBy similarity
Modified residuei504 – 5041PhosphoserineBy similarity
Modified residuei530 – 5301PhosphoserineCombined sources
Modified residuei537 – 5371PhosphoserineCombined sources
Modified residuei1028 – 10281PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ5SWW4.
MaxQBiQ5SWW4.
PaxDbiQ5SWW4.
PRIDEiQ5SWW4.

PTM databases

iPTMnetiQ5SWW4.
PhosphoSiteiQ5SWW4.

Expressioni

Gene expression databases

BgeeiQ5SWW4.
CleanExiMM_MED13.
GenevisibleiQ5SWW4. MM.

Interactioni

Subunit structurei

Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi236522. 1 interaction.
DIPiDIP-61082N.
IntActiQ5SWW4. 3 interactions.
STRINGi10090.ENSMUSP00000044268.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi1187 – 11915LXXLL motif 1
Motifi1278 – 12825LXXLL motif 2

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi448 – 4514Poly-Gln
Compositional biasi1507 – 160094Ser-richAdd
BLAST

Sequence similaritiesi

Belongs to the Mediator complex subunit 13 family.Curated

Phylogenomic databases

eggNOGiKOG3600. Eukaryota.
ENOG410XPV2. LUCA.
GeneTreeiENSGT00390000013680.
HOGENOMiHOG000012893.
HOVERGENiHBG058069.
InParanoidiQ5SWW4.
KOiK15164.
OMAiTGIKWKK.
OrthoDBiEOG73FQKS.
PhylomeDBiQ5SWW4.
TreeFamiTF316867.

Family and domain databases

InterProiIPR009401. Mediator_Med13.
IPR021643. Mediator_Med13_N_met/fun.
[Graphical view]
PfamiPF06333. Med13_C. 1 hit.
PF11597. Med13_N. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5SWW4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSSFVSNGA SLEDCHCNLF CLADLTGIKW KRYVWQGPTS APILFPVTEE
60 70 80 90 100
DPILSSFSRC LKADVLGVWR RDQRPGRREL WIFWWGKDPN FADLIHHDLS
110 120 130 140 150
EEEDGVWENG LSYECRTLLF KAVHNLLERC LMNRNFVRIG KWFVKPYEKD
160 170 180 190 200
EKPINKSEHL SCSFTFFLHG DSNVCTSVEI NQHQPVYLLS EEHVTLAQQS
210 220 230 240 250
NSPFQVILSP FGLNGTLTGQ AFKMSDSATK KLIGEWKQFY PISCGLKEMS
260 270 280 290 300
EEKQDDMDWE DDSLAAVEVL VAGVRMIYPA CFVLVPQSDI PAPSSVGASH
310 320 330 340 350
CSASCLGIHQ VPASTRDPAM SSVTLTPPTS PEEVQTVDPQ SAQKWVKFSS
360 370 380 390 400
VSDGFSTDST SHHGGKIPRK LANHVVDRVW QECNMNRLQN KRKYSATSSG
410 420 430 440 450
LCEEETADKI GCWDFVEATQ RTSCSCLRHK SLKTRNTGQQ GQAPSLGQQQ
460 470 480 490 500
QVLPKHKTNE KQDKSEKPQK RPLTPFHHRV SVSDEIGMDT DSASQRLVIS
510 520 530 540 550
AADSQVRFSN IRTNDVAKTP QMHGTELANS PQPPPLSPHP CDVVDEGVTK
560 570 580 590 600
TPSTPQSQHF YQMPTPDPLV PTKPMEDRID SLSQSFPPPF QEAVEPTVYV
610 620 630 640 650
GTAVSLEEDE ANVAWKYYKV PKKKDVEFLP PQLPNDKFKD DPVGPFGQES
660 670 680 690 700
VTSVTELMVQ CKKPLKVSDE IVQQYQIKNQ YLSAIASDTE QEPKIDPYAF
710 720 730 740 750
VEGDEEFIFT DKKDRQNSER EAGKKHKVED GTSAVTVLSH EEDAMSLFSP
760 770 780 790 800
SKQDAPRPTN HARPPSTSLI YDSDLAVSYT DLDNLFNSDE DELTPGSKKS
810 820 830 840 850
ASGSDDKASS KESKTGNLDP LSCISTADLH KMYPTPPSLE QHIMGFSPMN
860 870 880 890 900
MNNKEYGSVD TAPGGTVLEG NSSSVGTQFR IEVEEGFCSP KPSEIKDFSY
910 920 930 940 950
VYKPENCQVL VGCSMFAPLK TLPSHCLPPI KLPEECVYRQ SWTVGKLDLL
960 970 980 990 1000
PSGPSMPFIK EGDGSNLDQD YGPAYTPQTH ASFGMPPSSA PPSNGGAGIL
1010 1020 1030 1040 1050
PSPSTPRFPT PRTPRTPRTP RGAGGPASAQ GSVKYENSDL YSPASTPSTC
1060 1070 1080 1090 1100
RPLNSVEPAT VPSIPEAHSL YVNLILSESV MNLFKDCNFD SCCICVCNMN
1110 1120 1130 1140 1150
IKGADVGVYI PDPTQEAQYR CTCGFSAVMN RKFGNNSGLF LEDELDIIGR
1160 1170 1180 1190 1200
NTDCGKEAEK RFEALRASSV ENVNGGLKES EKVPDELILL LQDQCTNLFS
1210 1220 1230 1240 1250
PFGAADQDPF PKVGISSNWV RVEERDCCSD CCLALEHGRQ FMDNMSGGKV
1260 1270 1280 1290 1300
DEALVRSSCL HPWAKQNDAS VQCSQDILRM LLSLQPVLQD AIQKKRTVRP
1310 1320 1330 1340 1350
WGVQGPLTWQ QFHKMAGRGS YGTDESPEPL PIPTFLLGYD YDFLVLSPFA
1360 1370 1380 1390 1400
LPYWEKLMLE PYGSQRDIAY VVLCPENEAL LNGARSFFRD LTAIYESCRL
1410 1420 1430 1440 1450
GQHRPISRLL TDGIMKVGAT ASKKLSEKFV TEWFSQAADG NNEAFSKLKL
1460 1470 1480 1490 1500
YAQVCRYDLG PYLASQPLDS SLLSQPNLVA PPNQSLVTAP QMTNTGNANA
1510 1520 1530 1540 1550
PSATLASAAS STMTMTSGVP ISTSVATANS TLTTTSSSSS SSLSSGVSSN
1560 1570 1580 1590 1600
KLPSFPPFGS MNTSGTGSMS AQASTVQSGQ LGGQQSSSLQ AAGISGESAS
1610 1620 1630 1640 1650
LPTQPHPDVS ESTMDRDKVG IPTDGDSHAI TYPPAIVVYI IDPFTYENKD
1660 1670 1680 1690 1700
ESTNSSNVWT LGLLRCFLEM VQTLPPHIKS TVSVQIVPCQ YLLQPVKHDD
1710 1720 1730 1740 1750
RQIYSQHLKS LAFSVFTQCR RPLPTSTNVK TLTGFGPGLA METALKSPDR
1760 1770 1780 1790 1800
PECIRLYTPP FILAPVKDKQ TELGETFGEA GQKYNVLFVG YCLSHDQRWI
1810 1820 1830 1840 1850
LASCTDLYGE LLETCIINID VPNRARRKKG SARRFGLQKL WEWCLGLVQM
1860 1870 1880 1890 1900
SSLPWRVVIG RLGRIGHGEL KDWSCLLSRR NLQSLSKRLK DMCRMCGISA
1910 1920 1930 1940 1950
ADSPSILSAC LVAMEPQGSF VIMPDSVSTG SVFGRSTTLN MQTPQLNTPQ
1960 1970 1980 1990 2000
DTSCTHILVF PTSASVQVAS ATYTTENLDL AFNPNNDGAD GMGIFDLLDT
2010 2020 2030 2040 2050
GDDLDPDIIN ILPASPTASP VHSPGSHYPH GGDAGKGQGT DRLLSTESHD
2060 2070 2080 2090 2100
EVTNILQQPL ALGYFVSTAK AGPLPDWFWS ACPQAQYQCP LFLKASLHLH
2110 2120 2130 2140 2150
VPSVQSDELL HSKHSHPLDS NQTSDVLRFV LEQYNALSWL TCDPAVQDRR
2160 2170
SCLPVHFVVL NQLYNFIMNM L
Length:2,171
Mass (Da):238,591
Last modified:December 21, 2004 - v1
Checksum:iFD54462F5DC387B9
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti1299 – 132224RPWGV…RGSYG → GTDESAVVPEIHQFHKSLGQ PFYE in BAC97978 (PubMed:14621295).CuratedAdd
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL596256, AL592065 Genomic DNA. Translation: CAI24772.1.
AL592065, AL596256 Genomic DNA. Translation: CAI25862.1.
AK037303 mRNA. Translation: BAE20510.1.
AK129168 mRNA. Translation: BAC97978.1.
CCDSiCCDS36267.1.
RefSeqiNP_001074400.1. NM_001080931.1.
UniGeneiMm.341886.

Genome annotation databases

EnsembliENSMUST00000043624; ENSMUSP00000044268; ENSMUSG00000034297.
GeneIDi327987.
KEGGimmu:327987.
UCSCiuc007ksk.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL596256, AL592065 Genomic DNA. Translation: CAI24772.1.
AL592065, AL596256 Genomic DNA. Translation: CAI25862.1.
AK037303 mRNA. Translation: BAE20510.1.
AK129168 mRNA. Translation: BAC97978.1.
CCDSiCCDS36267.1.
RefSeqiNP_001074400.1. NM_001080931.1.
UniGeneiMm.341886.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi236522. 1 interaction.
DIPiDIP-61082N.
IntActiQ5SWW4. 3 interactions.
STRINGi10090.ENSMUSP00000044268.

PTM databases

iPTMnetiQ5SWW4.
PhosphoSiteiQ5SWW4.

Proteomic databases

EPDiQ5SWW4.
MaxQBiQ5SWW4.
PaxDbiQ5SWW4.
PRIDEiQ5SWW4.

Protocols and materials databases

DNASUi327987.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000043624; ENSMUSP00000044268; ENSMUSG00000034297.
GeneIDi327987.
KEGGimmu:327987.
UCSCiuc007ksk.1. mouse.

Organism-specific databases

CTDi9969.
MGIiMGI:3029632. Med13.
RougeiSearch...

Phylogenomic databases

eggNOGiKOG3600. Eukaryota.
ENOG410XPV2. LUCA.
GeneTreeiENSGT00390000013680.
HOGENOMiHOG000012893.
HOVERGENiHBG058069.
InParanoidiQ5SWW4.
KOiK15164.
OMAiTGIKWKK.
OrthoDBiEOG73FQKS.
PhylomeDBiQ5SWW4.
TreeFamiTF316867.

Enzyme and pathway databases

ReactomeiR-MMU-212436. Generic Transcription Pathway.
R-MMU-381340. Transcriptional regulation of white adipocyte differentiation.
R-MMU-442533. Transcriptional Regulation of Adipocyte Differentiation in 3T3-L1 Pre-adipocytes.

Miscellaneous databases

ChiTaRSiMed13. mouse.
PROiQ5SWW4.
SOURCEiSearch...

Gene expression databases

BgeeiQ5SWW4.
CleanExiMM_MED13.
GenevisibleiQ5SWW4. MM.

Family and domain databases

InterProiIPR009401. Mediator_Med13.
IPR021643. Mediator_Med13_N_met/fun.
[Graphical view]
PfamiPF06333. Med13_C. 1 hit.
PF11597. Med13_N. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 931-2171.
    Strain: C57BL/6J.
    Tissue: Thymus.
  3. "Prediction of the coding sequences of mouse homologues of KIAA gene: III. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
    Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S., Saga Y., Nagase T., Ohara O., Koga H.
    DNA Res. 10:167-180(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 803-2029.
    Tissue: Brain.
  4. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-530 AND SER-537, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Kidney, Lung, Spleen and Testis.

Entry informationi

Entry nameiMED13_MOUSE
AccessioniPrimary (citable) accession number: Q5SWW4
Secondary accession number(s): Q3V3P6, Q6ZQ90
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: December 21, 2004
Last modified: June 8, 2016
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.