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Protein

Platelet-activating factor acetylhydrolase IB subunit alpha

Gene

Pafah1b1

Organism
Mus musculus (Mouse)
Status
Unreviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Positively regulates the activity of the minus-end directed microtubule motor protein dynein. May enhance dynein-mediated microtubule sliding by targeting dynein to the microtubule plus end. Required for several dynein- and microtubule-dependent processes such as the maintenance of Golgi integrity, the peripheral transport of microtubule fragments and the coupling of the nucleus and centrosome. Required during brain development for the proliferation of neuronal precursors and the migration of newly formed neurons from the ventricular/subventricular zone toward the cortical plate. Neuronal migration involves a process called nucleokinesis, whereby migrating cells extend an anterior process into which the nucleus subsequently translocates. During nucleokinesis dynein at the nuclear surface may translocate the nucleus towards the centrosome by exerting force on centrosomal microtubules. Also required for proper activation of Rho GTPases and actin polymerization at the leading edge of locomoting cerebellar neurons and postmigratory hippocampal neurons in response to calcium influx triggered via NMDA receptors. May also play a role in other forms of cell locomotion including the migration of fibroblasts during wound healing. Non-catalytic subunit of an acetylhydrolase complex which inactivates platelet-activating factor (PAF) by removing the acetyl group at the SN-2 position.UniRule annotation

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDevelopmental proteinUniRule annotation
Biological processCell cycle, Cell division, DifferentiationUniRule annotation, Lipid degradationUniRule annotation, Lipid metabolism, MitosisUniRule annotationSAAS annotation, NeurogenesisUniRule annotation, TransportUniRule annotationSAAS annotation

Names & Taxonomyi

Protein namesi
Recommended name:
Platelet-activating factor acetylhydrolase IB subunit alphaUniRule annotation
Alternative name(s):
Lissencephaly-1 proteinUniRule annotation
Short name:
LIS-1UniRule annotation
PAF acetylhydrolase 45 kDa subunitUniRule annotation
Short name:
PAF-AH 45 kDa subunitUniRule annotation
PAF-AH alphaUniRule annotation
Short name:
PAFAH alphaUniRule annotation
Gene namesi
Name:Pafah1b1Imported
Synonyms:LIS1UniRule annotation, PAFAH1B1UniRule annotation
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus

Organism-specific databases

MGIiMGI:109520. Pafah1b1.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, CytoskeletonUniRule annotationSAAS annotation, MembraneUniRule annotation, MicrotubuleUniRule annotationSAAS annotation, NucleusUniRule annotation

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedUniRule annotation

Proteomic databases

MaxQBiQ5SW18.

Expressioni

Gene expression databases

BgeeiENSMUSG00000020745.

Interactioni

Subunit structurei

Can self-associate. Interacts with DCX, dynein, dynactin, IQGAP1, KATNB1, NDE1, NDEL1, NUDC and RSN. Interacts with DISC1, and this interaction is enhanced by NDEL1. Interacts with DAB1 when DAB1 is phosphorylated in response to RELN/reelin signaling. Component of cytosolic PAF-AH IB, which is composed of PAFAH1B1 (alpha), PAFAH1B2 (beta) and PAFAH1B3 (gamma) subunits. Trimer formation is not essential for the catalytic activity of the enzyme which is contributed solely by the PAFAH1B2 (beta) and PAFAH1B3 (gamma) subunits.UniRule annotation

GO - Molecular functioni

Structurei

3D structure databases

SMRiQ5SW18.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini7 – 39LisHInterPro annotationAdd BLAST33
Domaini104 – 410WD_REPEATS_REGIONInterPro annotationAdd BLAST307
Repeati104 – 145WDPROSITE-ProRule annotationAdd BLAST42
Repeati146 – 187WDPROSITE-ProRule annotationAdd BLAST42
Repeati188 – 229WDPROSITE-ProRule annotationAdd BLAST42
Repeati230 – 271WDPROSITE-ProRule annotationAdd BLAST42
Repeati308 – 333WDPROSITE-ProRule annotationAdd BLAST26
Repeati334 – 375WDPROSITE-ProRule annotationAdd BLAST42
Repeati376 – 410WDPROSITE-ProRule annotationAdd BLAST35

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 102Interaction with NDEL1UniRule annotationAdd BLAST102
Regioni1 – 66Interaction with NDE1UniRule annotationAdd BLAST66
Regioni1 – 38Required for self-association and interaction with PAFAH1B2 and PAFAH1B3UniRule annotationAdd BLAST38
Regioni83 – 410Interaction with dynein and dynactinUniRule annotationAdd BLAST328
Regioni388 – 410Interaction with NDEL1UniRule annotationAdd BLAST23

Domaini

Dimerization mediated by the LisH domain may be required to activate dynein.UniRule annotation

Sequence similaritiesi

Belongs to the WD repeat LIS1/nudF family.UniRule annotationSAAS annotation

Keywords - Domaini

Coiled coilUniRule annotationSAAS annotation, RepeatSAAS annotation, WD repeatPROSITE-ProRule annotationSAAS annotation

Phylogenomic databases

eggNOGiKOG0295. Eukaryota.
ENOG410XP3K. LUCA.
HOVERGENiHBG006271.
KOiK16794.
OMAiDAHGHFV.
OrthoDBiEOG091G07Q1.

Family and domain databases

HAMAPiMF_03141. lis1. 1 hit.
InterProiView protein in InterPro
IPR017252. Dynein_regulator_LIS1.
IPR020472. G-protein_beta_WD-40_rep.
IPR006594. LisH.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
PfamiView protein in Pfam
PF08513. LisH. 1 hit.
PF00400. WD40. 7 hits.
PIRSFiPIRSF037647. Dynein_regulator_Lis1. 1 hit.
PRINTSiPR00320. GPROTEINBRPT.
SMARTiView protein in SMART
SM00667. LisH. 1 hit.
SM00320. WD40. 7 hits.
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiView protein in PROSITE
PS50896. LISH. 1 hit.
PS00678. WD_REPEATS_1. 4 hits.
PS50082. WD_REPEATS_2. 7 hits.
PS50294. WD_REPEATS_REGION. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q5SW18-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVLSQRQRDE LNRAIADYLR SNGYEEAYSV FKKEAELDMN EELDKKYAGL
60 70 80 90 100
LEKKWTSVIR LQKKVMELES KLNEAKEEFT SGGPLGQKRD PKEWIPRPPE
110 120 130 140 150
KYALSGHRSP VTRVIFHPVF SVMVSASEDA TIKVWDYETG DFERTLKGHT
160 170 180 190 200
DSVQDISFDH SGKLLASCSA DMTIKLWDFQ GFECIRTMHG HDHNVSSVAI
210 220 230 240 250
MPNGDHIVSA SRDKTIKMWE VQTGYCVKTF TGHREWVRMV RPNQDGTLIA
260 270 280 290 300
SCSNDQTVRV WVVATKECKA ELREHEHVVE CISWAPESSY SSISEATGSE
310 320 330 340 350
TKKSGKPGPF LLSGSRDKTI KMWDVSTGMC LMTLVGHDNW VRGVLFHSGG
360 370 380 390 400
KFILSCADDK TLRVWDYKNK RCMKTLNAHE HFVTSLDFHK TAPYVVTGSV
410
DQTVKVWECR
Length:410
Mass (Da):46,670
Last modified:May 10, 2005 - v1
Checksum:i4DBF6A24A6B131CD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK144908 mRNA. Translation: BAE26129.1.
AK147207 mRNA. Translation: BAE27764.1.
AK147237 mRNA. Translation: BAE27787.1.
AK147507 mRNA. Translation: BAE27961.1.
AK147533 mRNA. Translation: BAE27977.1.
AK164008 mRNA. Translation: BAE37586.1.
AK168929 mRNA. Translation: BAE40742.1.
AK168960 mRNA. Translation: BAE40766.1.
RefSeqiNP_038653.1. NM_013625.4.
UniGeneiMm.397111.

Genome annotation databases

GeneIDi18472.
KEGGimmu:18472.

Similar proteinsi

Entry informationi

Entry nameiQ5SW18_MOUSE
AccessioniPrimary (citable) accession number: Q5SW18
Entry historyiIntegrated into UniProtKB/TrEMBL: May 10, 2005
Last sequence update: May 10, 2005
Last modified: September 27, 2017
This is version 131 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)