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Q5SW18

- Q5SW18_MOUSE

UniProt

Q5SW18 - Q5SW18_MOUSE

Protein

Platelet-activating factor acetylhydrolase IB subunit alpha

Gene

Pafah1b1

Organism
Mus musculus (Mouse)
Status
Unreviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 100 (01 Oct 2014)
      Sequence version 1 (10 May 2005)
      Previous versions | rss
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    Functioni

    Positively regulates the activity of the minus-end directed microtubule motor protein dynein. May enhance dynein-mediated microtubule sliding by targeting dynein to the microtubule plus end. Required for several dynein- and microtubule-dependent processes such as the maintenance of Golgi integrity, the peripheral transport of microtubule fragments and the coupling of the nucleus and centrosome. Required during brain development for the proliferation of neuronal precursors and the migration of newly formed neurons from the ventricular/subventricular zone toward the cortical plate. Neuronal migration involves a process called nucleokinesis, whereby migrating cells extend an anterior process into which the nucleus subsequently translocates. During nucleokinesis dynein at the nuclear surface may translocate the nucleus towards the centrosome by exerting force on centrosomal microtubules. Also required for proper activation of Rho GTPases and actin polymerization at the leading edge of locomoting cerebellar neurons and postmigratory hippocampal neurons in response to calcium influx triggered via NMDA receptors. May also play a role in other forms of cell locomotion including the migration of fibroblasts during wound healing. Non-catalytic subunit of an acetylhydrolase complex which inactivates platelet-activating factor (PAF) by removing the acetyl group at the SN-2 position.UniRule annotation

    GO - Molecular functioni

    1. microtubule binding Source: MGI
    2. phosphoprotein binding Source: MGI
    3. protein binding Source: MGI
    4. protein homodimerization activity Source: MGI

    GO - Biological processi

    1. acrosome assembly Source: MGI
    2. actin cytoskeleton organization Source: MGI
    3. adult locomotory behavior Source: MGI
    4. ameboidal cell migration Source: MGI
    5. brain morphogenesis Source: Ensembl
    6. cell migration Source: MGI
    7. cerebral cortex development Source: MGI
    8. cerebral cortex neuron differentiation Source: Ensembl
    9. corpus callosum morphogenesis Source: Ensembl
    10. establishment of centrosome localization Source: Ensembl
    11. establishment of mitotic spindle orientation Source: UniProtKB-HAMAP
    12. hippocampus development Source: MGI
    13. layer formation in cerebral cortex Source: MGI
    14. learning or memory Source: MGI
    15. lipid catabolic process Source: UniProtKB-KW
    16. microtubule cytoskeleton organization Source: MGI
    17. microtubule organizing center organization Source: Ensembl
    18. mitotic nuclear division Source: UniProtKB-KW
    19. negative regulation of JNK cascade Source: MGI
    20. negative regulation of neuron projection development Source: Ensembl
    21. neuroblast proliferation Source: MGI
    22. neuromuscular process controlling balance Source: MGI
    23. neuron migration Source: MGI
    24. nuclear envelope disassembly Source: MGI
    25. nuclear migration Source: Ensembl
    26. osteoclast development Source: MGI
    27. positive regulation of axon extension Source: Ensembl
    28. positive regulation of cytokine-mediated signaling pathway Source: MGI
    29. positive regulation of mitotic cell cycle Source: Ensembl
    30. protein secretion Source: MGI
    31. regulation of Rho GTPase activity Source: MGI
    32. retrograde axon cargo transport Source: MGI
    33. stem cell division Source: Ensembl
    34. synaptic transmission Source: MGI
    35. transmission of nerve impulse Source: MGI
    36. vesicle transport along microtubule Source: MGI

    Keywords - Molecular functioni

    Developmental proteinUniRule annotation

    Keywords - Biological processi

    Cell cycle, Cell division, DifferentiationUniRule annotation, Lipid degradationUniRule annotation, Lipid metabolism, MitosisUniRule annotationSAAS annotation, NeurogenesisUniRule annotation, TransportUniRule annotationSAAS annotation

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Platelet-activating factor acetylhydrolase IB subunit alphaUniRule annotation
    Alternative name(s):
    Lissencephaly-1 proteinUniRule annotation
    PAF acetylhydrolase 45 kDa subunitUniRule annotation
    PAF-AH alphaUniRule annotation
    Gene namesi
    Name:Pafah1b1UniRule annotationImported
    Synonyms:Lis1UniRule annotation
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

    Organism-specific databases

    MGIiMGI:109520. Pafah1b1.

    Subcellular locationi

    Cytoplasmcytoskeleton UniRule annotation. Cytoplasmcytoskeletonmicrotubule organizing centercentrosome UniRule annotation. Cytoplasmcytoskeletonspindle UniRule annotation. Nucleus membrane UniRule annotation
    Note: Localizes to the plus end of microtubules and to the centrosome. May localize to the nuclear membrane. Redistributes to axons during neuronal development. Also localizes to the microtubules of the manchette in elongating spermatids and to the meiotic spindle in spermatocytes.UniRule annotation

    GO - Cellular componenti

    1. astral microtubule Source: Ensembl
    2. cell cortex Source: Ensembl
    3. centrosome Source: MGI
    4. cytoplasm Source: MGI
    5. cytosol Source: Ensembl
    6. growth cone Source: Ensembl
    7. kinesin complex Source: Ensembl
    8. kinetochore Source: Ensembl
    9. microtubule associated complex Source: MGI
    10. microtubule cytoskeleton Source: MGI
    11. motile primary cilium Source: MGI
    12. neuronal cell body Source: Ensembl
    13. nuclear envelope Source: MGI
    14. nuclear membrane Source: UniProtKB-SubCell
    15. perinuclear region of cytoplasm Source: MGI
    16. vesicle Source: Ensembl

    Keywords - Cellular componenti

    Cytoplasm, CytoskeletonUniRule annotationSAAS annotation, MembraneUniRule annotation, MicrotubuleUniRule annotationSAAS annotation, NucleusUniRule annotation

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedUniRule annotation

    Expressioni

    Gene expression databases

    GenevestigatoriQ5SW18.

    Interactioni

    Subunit structurei

    Can self-associate. Interacts with DCX, dynein, dynactin, IQGAP1, KATNB1, NDE1, NDEL1, NUDC and RSN. Interacts with DISC1, and this interaction is enhanced by NDEL1. Interacts with DAB1 when DAB1 is phosphorylated in response to RELN/reelin signaling. Component of cytosolic PAF-AH IB, which is composed of PAFAH1B1 (alpha), PAFAH1B2 (beta) and PAFAH1B3 (gamma) subunits. Trimer formation is not essential for the catalytic activity of the enzyme which is contributed solely by the PAFAH1B2 (beta) and PAFAH1B3 (gamma) subunits.UniRule annotation

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini7 – 3933LisHUniRule annotationAdd
    BLAST
    Repeati106 – 14742WD 1UniRule annotationAdd
    BLAST
    Repeati148 – 18740WD 2UniRule annotationAdd
    BLAST
    Repeati190 – 22940WD 3UniRule annotationAdd
    BLAST
    Repeati232 – 27140WD 4UniRule annotationAdd
    BLAST
    Repeati274 – 33360WD 5UniRule annotationAdd
    BLAST
    Repeati336 – 37742WD 6UniRule annotationAdd
    BLAST
    Repeati378 – 41033WD 7UniRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 102102Interaction with NDEL1UniRule annotationAdd
    BLAST
    Regioni1 – 6666Interaction with NDE1UniRule annotationAdd
    BLAST
    Regioni1 – 3838Required for self-association and interaction with PAFAH1B2 and PAFAH1B3UniRule annotationAdd
    BLAST
    Regioni83 – 410328Interaction with dynein and dynactinUniRule annotationAdd
    BLAST
    Regioni367 – 40943Interaction with DCXUniRule annotationAdd
    BLAST
    Regioni388 – 41023Interaction with NDEL1UniRule annotationAdd
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili56 – 8227UniRule annotationAdd
    BLAST

    Domaini

    Dimerization mediated by the LisH domain may be required to activate dynein.UniRule annotation

    Sequence similaritiesi

    Belongs to the WD repeat LIS1/nudF family.UniRule annotation
    Contains 1 LisH domain.UniRule annotation
    Contains 7 WD repeats.UniRule annotation
    Contains LisH domain.SAAS annotation

    Keywords - Domaini

    Coiled coilUniRule annotationSAAS annotation, RepeatUniRule annotationSAAS annotation, WD repeatUniRule annotationSAAS annotation

    Phylogenomic databases

    GeneTreeiENSGT00750000117266.
    HOVERGENiHBG006271.
    InParanoidiQ5SW18.
    KOiK16794.
    OMAiMKTLYAH.

    Family and domain databases

    Gene3Di2.130.10.10. 1 hit.
    HAMAPiMF_03141. lis1.
    InterProiIPR017252. Dynein_regulator_LIS1.
    IPR020472. G-protein_beta_WD-40_rep.
    IPR006594. LisH_dimerisation.
    IPR013720. LisH_dimerisation_subgr.
    IPR015943. WD40/YVTN_repeat-like_dom.
    IPR001680. WD40_repeat.
    IPR019775. WD40_repeat_CS.
    IPR017986. WD40_repeat_dom.
    [Graphical view]
    PfamiPF08513. LisH. 1 hit.
    PF00400. WD40. 7 hits.
    [Graphical view]
    PIRSFiPIRSF037647. Dynein_regulator_Lis1. 1 hit.
    PRINTSiPR00320. GPROTEINBRPT.
    SMARTiSM00667. LisH. 1 hit.
    SM00320. WD40. 7 hits.
    [Graphical view]
    SUPFAMiSSF50978. SSF50978. 1 hit.
    PROSITEiPS50896. LISH. 1 hit.
    PS00678. WD_REPEATS_1. 4 hits.
    PS50082. WD_REPEATS_2. 7 hits.
    PS50294. WD_REPEATS_REGION. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q5SW18-1 [UniParc]FASTAAdd to Basket

    « Hide

    MVLSQRQRDE LNRAIADYLR SNGYEEAYSV FKKEAELDMN EELDKKYAGL    50
    LEKKWTSVIR LQKKVMELES KLNEAKEEFT SGGPLGQKRD PKEWIPRPPE 100
    KYALSGHRSP VTRVIFHPVF SVMVSASEDA TIKVWDYETG DFERTLKGHT 150
    DSVQDISFDH SGKLLASCSA DMTIKLWDFQ GFECIRTMHG HDHNVSSVAI 200
    MPNGDHIVSA SRDKTIKMWE VQTGYCVKTF TGHREWVRMV RPNQDGTLIA 250
    SCSNDQTVRV WVVATKECKA ELREHEHVVE CISWAPESSY SSISEATGSE 300
    TKKSGKPGPF LLSGSRDKTI KMWDVSTGMC LMTLVGHDNW VRGVLFHSGG 350
    KFILSCADDK TLRVWDYKNK RCMKTLNAHE HFVTSLDFHK TAPYVVTGSV 400
    DQTVKVWECR 410
    Length:410
    Mass (Da):46,670
    Last modified:May 10, 2005 - v1
    Checksum:i4DBF6A24A6B131CD
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK144908 mRNA. Translation: BAE26129.1.
    AK147207 mRNA. Translation: BAE27764.1.
    AK147237 mRNA. Translation: BAE27787.1.
    AK147507 mRNA. Translation: BAE27961.1.
    AK147533 mRNA. Translation: BAE27977.1.
    AK164008 mRNA. Translation: BAE37586.1.
    AK168929 mRNA. Translation: BAE40742.1.
    AK168960 mRNA. Translation: BAE40766.1.
    RefSeqiNP_038653.1. NM_013625.4.
    UniGeneiMm.397111.

    Genome annotation databases

    GeneIDi18472.
    KEGGimmu:18472.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK144908 mRNA. Translation: BAE26129.1 .
    AK147207 mRNA. Translation: BAE27764.1 .
    AK147237 mRNA. Translation: BAE27787.1 .
    AK147507 mRNA. Translation: BAE27961.1 .
    AK147533 mRNA. Translation: BAE27977.1 .
    AK164008 mRNA. Translation: BAE37586.1 .
    AK168929 mRNA. Translation: BAE40742.1 .
    AK168960 mRNA. Translation: BAE40766.1 .
    RefSeqi NP_038653.1. NM_013625.4.
    UniGenei Mm.397111.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 18472.
    KEGGi mmu:18472.

    Organism-specific databases

    CTDi 5048.
    MGIi MGI:109520. Pafah1b1.

    Phylogenomic databases

    GeneTreei ENSGT00750000117266.
    HOVERGENi HBG006271.
    InParanoidi Q5SW18.
    KOi K16794.
    OMAi MKTLYAH.

    Miscellaneous databases

    ChiTaRSi PAFAH1B1. mouse.
    NextBioi 294162.
    SOURCEi Search...

    Gene expression databases

    Genevestigatori Q5SW18.

    Family and domain databases

    Gene3Di 2.130.10.10. 1 hit.
    HAMAPi MF_03141. lis1.
    InterProi IPR017252. Dynein_regulator_LIS1.
    IPR020472. G-protein_beta_WD-40_rep.
    IPR006594. LisH_dimerisation.
    IPR013720. LisH_dimerisation_subgr.
    IPR015943. WD40/YVTN_repeat-like_dom.
    IPR001680. WD40_repeat.
    IPR019775. WD40_repeat_CS.
    IPR017986. WD40_repeat_dom.
    [Graphical view ]
    Pfami PF08513. LisH. 1 hit.
    PF00400. WD40. 7 hits.
    [Graphical view ]
    PIRSFi PIRSF037647. Dynein_regulator_Lis1. 1 hit.
    PRINTSi PR00320. GPROTEINBRPT.
    SMARTi SM00667. LisH. 1 hit.
    SM00320. WD40. 7 hits.
    [Graphical view ]
    SUPFAMi SSF50978. SSF50978. 1 hit.
    PROSITEi PS50896. LISH. 1 hit.
    PS00678. WD_REPEATS_1. 4 hits.
    PS50082. WD_REPEATS_2. 7 hits.
    PS50294. WD_REPEATS_REGION. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "High-efficiency full-length cDNA cloning."
      Carninci P., Hayashizaki Y.
      Methods Enzymol. 303:19-44(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE.
      Strain: C57BL/6Imported and C57BL/6JImported.
      Tissue: AmnionImported, BrainImported, Mammary glandImported, StomachImported and Whole bodyImported.
    2. "Normalization and subtraction of cap-trapper-selected cDNAs to prepare full-length cDNA libraries for rapid discovery of new genes."
      Carninci P., Shibata Y., Hayatsu N., Sugahara Y., Shibata K., Itoh M., Konno H., Okazaki Y., Muramatsu M., Hayashizaki Y.
      Genome Res. 10:1617-1630(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE.
      Strain: C57BL/6Imported and C57BL/6JImported.
      Tissue: AmnionImported, BrainImported, Mammary glandImported, StomachImported and Whole bodyImported.
    3. Cited for: NUCLEOTIDE SEQUENCE.
      Strain: C57BL/6Imported and C57BL/6JImported.
      Tissue: AmnionImported, BrainImported, Mammary glandImported, StomachImported and Whole bodyImported.
    4. "Functional annotation of a full-length mouse cDNA collection."
      The RIKEN Genome Exploration Research Group Phase II Team and the FANTOM Consortium
      Nature 409:685-690(2001)
      Cited for: NUCLEOTIDE SEQUENCE.
      Strain: C57BL/6Imported and C57BL/6JImported.
      Tissue: AmnionImported, BrainImported, Mammary glandImported, StomachImported and Whole bodyImported.
    5. "Analysis of the mouse transcriptome based on functional annotation of 60,770 full-length cDNAs."
      The FANTOM Consortium and the RIKEN Genome Exploration Research Group Phase I and II Team
      Nature 420:563-573(2002)
      Cited for: NUCLEOTIDE SEQUENCE.
      Strain: C57BL/6Imported and C57BL/6JImported.
      Tissue: AmnionImported, BrainImported, Mammary glandImported, StomachImported and Whole bodyImported.
    6. Cited for: NUCLEOTIDE SEQUENCE.
      Strain: C57BL/6Imported and C57BL/6JImported.
      Tissue: BrainImported, Mammary glandImported and Whole bodyImported.
    7. Cited for: NUCLEOTIDE SEQUENCE.
      Strain: C57BL/6JImported.
      Tissue: AmnionImported, StomachImported and Whole bodyImported.
    8. "The Transcriptional Landscape of the Mammalian Genome."
      The FANTOM Consortium, Riken Genome Exploration Research Group and Genome Science Group (Genome Network Project Core Group)
      Science 309:1559-1563(2005)
      Cited for: NUCLEOTIDE SEQUENCE.
      Strain: C57BL/6Imported and C57BL/6JImported.
      Tissue: AmnionImported, BrainImported, Mammary glandImported, StomachImported and Whole bodyImported.
    9. Cited for: NUCLEOTIDE SEQUENCE.
      Strain: C57BL/6Imported and C57BL/6JImported.
      Tissue: AmnionImported, BrainImported, Mammary glandImported, StomachImported and Whole bodyImported.

    Entry informationi

    Entry nameiQ5SW18_MOUSE
    AccessioniPrimary (citable) accession number: Q5SW18
    Entry historyi
    Integrated into UniProtKB/TrEMBL: May 10, 2005
    Last sequence update: May 10, 2005
    Last modified: October 1, 2014
    This is version 100 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)