Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q5SW18

- Q5SW18_MOUSE

UniProt

Q5SW18 - Q5SW18_MOUSE

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Platelet-activating factor acetylhydrolase IB subunit alpha

Gene

Pafah1b1

Organism
Mus musculus (Mouse)
Status
Unreviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli

Functioni

Positively regulates the activity of the minus-end directed microtubule motor protein dynein. May enhance dynein-mediated microtubule sliding by targeting dynein to the microtubule plus end. Required for several dynein- and microtubule-dependent processes such as the maintenance of Golgi integrity, the peripheral transport of microtubule fragments and the coupling of the nucleus and centrosome. Required during brain development for the proliferation of neuronal precursors and the migration of newly formed neurons from the ventricular/subventricular zone toward the cortical plate. Neuronal migration involves a process called nucleokinesis, whereby migrating cells extend an anterior process into which the nucleus subsequently translocates. During nucleokinesis dynein at the nuclear surface may translocate the nucleus towards the centrosome by exerting force on centrosomal microtubules. Also required for proper activation of Rho GTPases and actin polymerization at the leading edge of locomoting cerebellar neurons and postmigratory hippocampal neurons in response to calcium influx triggered via NMDA receptors. May also play a role in other forms of cell locomotion including the migration of fibroblasts during wound healing. Non-catalytic subunit of an acetylhydrolase complex which inactivates platelet-activating factor (PAF) by removing the acetyl group at the SN-2 position.UniRule annotation

GO - Molecular functioni

  1. microtubule binding Source: MGI
  2. phosphoprotein binding Source: MGI
  3. protein homodimerization activity Source: MGI

GO - Biological processi

  1. acrosome assembly Source: MGI
  2. actin cytoskeleton organization Source: MGI
  3. adult locomotory behavior Source: MGI
  4. ameboidal cell migration Source: MGI
  5. brain morphogenesis Source: Ensembl
  6. cell migration Source: MGI
  7. cerebral cortex development Source: MGI
  8. cerebral cortex neuron differentiation Source: Ensembl
  9. corpus callosum morphogenesis Source: Ensembl
  10. establishment of centrosome localization Source: Ensembl
  11. establishment of mitotic spindle orientation Source: UniProtKB-HAMAP
  12. hippocampus development Source: MGI
  13. layer formation in cerebral cortex Source: MGI
  14. learning or memory Source: MGI
  15. lipid catabolic process Source: UniProtKB-KW
  16. microtubule cytoskeleton organization Source: MGI
  17. microtubule organizing center organization Source: Ensembl
  18. mitotic nuclear division Source: UniProtKB-KW
  19. negative regulation of JNK cascade Source: MGI
  20. negative regulation of neuron projection development Source: Ensembl
  21. neuroblast proliferation Source: MGI
  22. neuromuscular process controlling balance Source: MGI
  23. neuron migration Source: MGI
  24. nuclear envelope disassembly Source: MGI
  25. nuclear migration Source: Ensembl
  26. osteoclast development Source: MGI
  27. positive regulation of axon extension Source: Ensembl
  28. positive regulation of cytokine-mediated signaling pathway Source: MGI
  29. positive regulation of mitotic cell cycle Source: Ensembl
  30. protein secretion Source: MGI
  31. regulation of Rho GTPase activity Source: MGI
  32. retrograde axon cargo transport Source: MGI
  33. stem cell division Source: Ensembl
  34. synaptic transmission Source: MGI
  35. transmission of nerve impulse Source: MGI
  36. vesicle transport along microtubule Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Developmental proteinUniRule annotation

Keywords - Biological processi

Cell cycle, Cell division, DifferentiationUniRule annotation, Lipid degradationUniRule annotation, Lipid metabolism, MitosisUniRule annotationSAAS annotation, NeurogenesisUniRule annotation, TransportUniRule annotationSAAS annotation

Names & Taxonomyi

Protein namesi
Recommended name:
Platelet-activating factor acetylhydrolase IB subunit alphaUniRule annotation
Alternative name(s):
Lissencephaly-1 proteinUniRule annotation
PAF acetylhydrolase 45 kDa subunitUniRule annotation
PAF-AH alphaUniRule annotation
Gene namesi
Name:Pafah1b1UniRule annotationImported
Synonyms:Lis1UniRule annotation
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Organism-specific databases

MGIiMGI:109520. Pafah1b1.

Subcellular locationi

Cytoplasmcytoskeleton UniRule annotation. Cytoplasmcytoskeletonmicrotubule organizing centercentrosome UniRule annotation. Cytoplasmcytoskeletonspindle UniRule annotation. Nucleus membrane UniRule annotation
Note: Localizes to the plus end of microtubules and to the centrosome. May localize to the nuclear membrane. Redistributes to axons during neuronal development. Also localizes to the microtubules of the manchette in elongating spermatids and to the meiotic spindle in spermatocytes.UniRule annotation

GO - Cellular componenti

  1. astral microtubule Source: Ensembl
  2. cell cortex Source: Ensembl
  3. centrosome Source: MGI
  4. cytoplasm Source: MGI
  5. cytosol Source: Ensembl
  6. extracellular vesicular exosome Source: Ensembl
  7. growth cone Source: Ensembl
  8. kinesin complex Source: Ensembl
  9. kinetochore Source: Ensembl
  10. microtubule associated complex Source: MGI
  11. microtubule cytoskeleton Source: MGI
  12. motile primary cilium Source: MGI
  13. neuronal cell body Source: Ensembl
  14. nuclear envelope Source: MGI
  15. nuclear membrane Source: UniProtKB-SubCell
  16. perinuclear region of cytoplasm Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, CytoskeletonUniRule annotationSAAS annotation, MembraneUniRule annotation, MicrotubuleUniRule annotationSAAS annotation, NucleusUniRule annotation

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedUniRule annotation

Expressioni

Gene expression databases

GenevestigatoriQ5SW18.

Interactioni

Subunit structurei

Can self-associate. Interacts with DCX, dynein, dynactin, IQGAP1, KATNB1, NDE1, NDEL1, NUDC and RSN. Interacts with DISC1, and this interaction is enhanced by NDEL1. Interacts with DAB1 when DAB1 is phosphorylated in response to RELN/reelin signaling. Component of cytosolic PAF-AH IB, which is composed of PAFAH1B1 (alpha), PAFAH1B2 (beta) and PAFAH1B3 (gamma) subunits. Trimer formation is not essential for the catalytic activity of the enzyme which is contributed solely by the PAFAH1B2 (beta) and PAFAH1B3 (gamma) subunits.UniRule annotation

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini7 – 3933LisHUniRule annotationAdd
BLAST
Repeati106 – 14742WD 1UniRule annotationAdd
BLAST
Repeati148 – 18740WD 2UniRule annotationAdd
BLAST
Repeati190 – 22940WD 3UniRule annotationAdd
BLAST
Repeati232 – 27140WD 4UniRule annotationAdd
BLAST
Repeati274 – 33360WD 5UniRule annotationAdd
BLAST
Repeati336 – 37742WD 6UniRule annotationAdd
BLAST
Repeati378 – 41033WD 7UniRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 102102Interaction with NDEL1UniRule annotationAdd
BLAST
Regioni1 – 6666Interaction with NDE1UniRule annotationAdd
BLAST
Regioni1 – 3838Required for self-association and interaction with PAFAH1B2 and PAFAH1B3UniRule annotationAdd
BLAST
Regioni83 – 410328Interaction with dynein and dynactinUniRule annotationAdd
BLAST
Regioni367 – 40943Interaction with DCXUniRule annotationAdd
BLAST
Regioni388 – 41023Interaction with NDEL1UniRule annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili56 – 8227UniRule annotationAdd
BLAST

Domaini

Dimerization mediated by the LisH domain may be required to activate dynein.UniRule annotation

Sequence similaritiesi

Belongs to the WD repeat LIS1/nudF family.UniRule annotation
Contains 1 LisH domain.UniRule annotation
Contains 7 WD repeats.UniRule annotation
Contains LisH domain.SAAS annotation

Keywords - Domaini

Coiled coilUniRule annotationSAAS annotation, RepeatUniRule annotation, WD repeatUniRule annotation

Phylogenomic databases

GeneTreeiENSGT00760000118869.
HOVERGENiHBG006271.
KOiK16794.
OMAiMKTLYAH.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
HAMAPiMF_03141. lis1.
InterProiIPR017252. Dynein_regulator_LIS1.
IPR020472. G-protein_beta_WD-40_rep.
IPR006594. LisH_dimerisation.
IPR013720. LisH_dimerisation_subgr.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF08513. LisH. 1 hit.
PF00400. WD40. 7 hits.
[Graphical view]
PIRSFiPIRSF037647. Dynein_regulator_Lis1. 1 hit.
PRINTSiPR00320. GPROTEINBRPT.
SMARTiSM00667. LisH. 1 hit.
SM00320. WD40. 7 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS50896. LISH. 1 hit.
PS00678. WD_REPEATS_1. 4 hits.
PS50082. WD_REPEATS_2. 7 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q5SW18 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MVLSQRQRDE LNRAIADYLR SNGYEEAYSV FKKEAELDMN EELDKKYAGL
60 70 80 90 100
LEKKWTSVIR LQKKVMELES KLNEAKEEFT SGGPLGQKRD PKEWIPRPPE
110 120 130 140 150
KYALSGHRSP VTRVIFHPVF SVMVSASEDA TIKVWDYETG DFERTLKGHT
160 170 180 190 200
DSVQDISFDH SGKLLASCSA DMTIKLWDFQ GFECIRTMHG HDHNVSSVAI
210 220 230 240 250
MPNGDHIVSA SRDKTIKMWE VQTGYCVKTF TGHREWVRMV RPNQDGTLIA
260 270 280 290 300
SCSNDQTVRV WVVATKECKA ELREHEHVVE CISWAPESSY SSISEATGSE
310 320 330 340 350
TKKSGKPGPF LLSGSRDKTI KMWDVSTGMC LMTLVGHDNW VRGVLFHSGG
360 370 380 390 400
KFILSCADDK TLRVWDYKNK RCMKTLNAHE HFVTSLDFHK TAPYVVTGSV
410
DQTVKVWECR
Length:410
Mass (Da):46,670
Last modified:May 10, 2005 - v1
Checksum:i4DBF6A24A6B131CD
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK144908 mRNA. Translation: BAE26129.1.
AK147207 mRNA. Translation: BAE27764.1.
AK147237 mRNA. Translation: BAE27787.1.
AK147507 mRNA. Translation: BAE27961.1.
AK147533 mRNA. Translation: BAE27977.1.
AK164008 mRNA. Translation: BAE37586.1.
AK168929 mRNA. Translation: BAE40742.1.
AK168960 mRNA. Translation: BAE40766.1.
RefSeqiNP_038653.1. NM_013625.4.
UniGeneiMm.397111.

Genome annotation databases

GeneIDi18472.
KEGGimmu:18472.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK144908 mRNA. Translation: BAE26129.1 .
AK147207 mRNA. Translation: BAE27764.1 .
AK147237 mRNA. Translation: BAE27787.1 .
AK147507 mRNA. Translation: BAE27961.1 .
AK147533 mRNA. Translation: BAE27977.1 .
AK164008 mRNA. Translation: BAE37586.1 .
AK168929 mRNA. Translation: BAE40742.1 .
AK168960 mRNA. Translation: BAE40766.1 .
RefSeqi NP_038653.1. NM_013625.4.
UniGenei Mm.397111.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 18472.
KEGGi mmu:18472.

Organism-specific databases

CTDi 5048.
MGIi MGI:109520. Pafah1b1.

Phylogenomic databases

GeneTreei ENSGT00760000118869.
HOVERGENi HBG006271.
KOi K16794.
OMAi MKTLYAH.

Miscellaneous databases

ChiTaRSi PAFAH1B1. mouse.
NextBioi 294162.
SOURCEi Search...

Gene expression databases

Genevestigatori Q5SW18.

Family and domain databases

Gene3Di 2.130.10.10. 1 hit.
HAMAPi MF_03141. lis1.
InterProi IPR017252. Dynein_regulator_LIS1.
IPR020472. G-protein_beta_WD-40_rep.
IPR006594. LisH_dimerisation.
IPR013720. LisH_dimerisation_subgr.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view ]
Pfami PF08513. LisH. 1 hit.
PF00400. WD40. 7 hits.
[Graphical view ]
PIRSFi PIRSF037647. Dynein_regulator_Lis1. 1 hit.
PRINTSi PR00320. GPROTEINBRPT.
SMARTi SM00667. LisH. 1 hit.
SM00320. WD40. 7 hits.
[Graphical view ]
SUPFAMi SSF50978. SSF50978. 1 hit.
PROSITEi PS50896. LISH. 1 hit.
PS00678. WD_REPEATS_1. 4 hits.
PS50082. WD_REPEATS_2. 7 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "High-efficiency full-length cDNA cloning."
    Carninci P., Hayashizaki Y.
    Methods Enzymol. 303:19-44(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: C57BL/6Imported and C57BL/6JImported.
    Tissue: AmnionImported, BrainImported, Mammary glandImported, StomachImported and Whole bodyImported.
  2. "Normalization and subtraction of cap-trapper-selected cDNAs to prepare full-length cDNA libraries for rapid discovery of new genes."
    Carninci P., Shibata Y., Hayatsu N., Sugahara Y., Shibata K., Itoh M., Konno H., Okazaki Y., Muramatsu M., Hayashizaki Y.
    Genome Res. 10:1617-1630(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: C57BL/6Imported and C57BL/6JImported.
    Tissue: AmnionImported, BrainImported, Mammary glandImported, StomachImported and Whole bodyImported.
  3. Cited for: NUCLEOTIDE SEQUENCE.
    Strain: C57BL/6Imported and C57BL/6JImported.
    Tissue: AmnionImported, BrainImported, Mammary glandImported, StomachImported and Whole bodyImported.
  4. "Functional annotation of a full-length mouse cDNA collection."
    The RIKEN Genome Exploration Research Group Phase II Team and the FANTOM Consortium
    Nature 409:685-690(2001)
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: C57BL/6Imported and C57BL/6JImported.
    Tissue: AmnionImported, BrainImported, Mammary glandImported, StomachImported and Whole bodyImported.
  5. "Analysis of the mouse transcriptome based on functional annotation of 60,770 full-length cDNAs."
    The FANTOM Consortium and the RIKEN Genome Exploration Research Group Phase I and II Team
    Nature 420:563-573(2002)
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: C57BL/6Imported and C57BL/6JImported.
    Tissue: AmnionImported, BrainImported, Mammary glandImported, StomachImported and Whole bodyImported.
  6. Cited for: NUCLEOTIDE SEQUENCE.
    Strain: C57BL/6Imported and C57BL/6JImported.
    Tissue: BrainImported, Mammary glandImported and Whole bodyImported.
  7. Cited for: NUCLEOTIDE SEQUENCE.
    Strain: C57BL/6JImported.
    Tissue: AmnionImported, StomachImported and Whole bodyImported.
  8. "The Transcriptional Landscape of the Mammalian Genome."
    The FANTOM Consortium, Riken Genome Exploration Research Group and Genome Science Group (Genome Network Project Core Group)
    Science 309:1559-1563(2005)
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: C57BL/6Imported and C57BL/6JImported.
    Tissue: AmnionImported, BrainImported, Mammary glandImported, StomachImported and Whole bodyImported.
  9. Cited for: NUCLEOTIDE SEQUENCE.
    Strain: C57BL/6Imported and C57BL/6JImported.
    Tissue: AmnionImported, BrainImported, Mammary glandImported, StomachImported and Whole bodyImported.

Entry informationi

Entry nameiQ5SW18_MOUSE
AccessioniPrimary (citable) accession number: Q5SW18
Entry historyi
Integrated into UniProtKB/TrEMBL: May 10, 2005
Last sequence update: May 10, 2005
Last modified: October 29, 2014
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)