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Protein

Synergin gamma

Gene

Synrg

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

May play a role in endocytosis and/or membrane trafficking at the trans-Golgi network (TGN). May act by linking the adapter protein complex AP-1 to other proteins (By similarity).By similarity

GO - Molecular functioni

  1. calcium ion binding Source: InterPro

GO - Biological processi

  1. endocytosis Source: UniProtKB-KW
  2. protein transport Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Endocytosis, Protein transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Synergin gamma
Alternative name(s):
AP1 subunit gamma-binding protein 1
Gamma-synergin
Gene namesi
Name:Synrg
Synonyms:Ap1gbp1, Syng
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 11

Organism-specific databases

MGIiMGI:1354742. Synrg.

Subcellular locationi

Cytoplasmcytosol 1 Publication. Golgi apparatustrans-Golgi network membrane 1 Publication; Peripheral membrane protein 1 Publication
Note: Associated with membranes of the TGN, colocalizes with AP1G1 (By similarity). Associates with membranes via the adapter protein complex AP-1.By similarity

GO - Cellular componenti

  1. cytosol Source: UniProtKB-SubCell
  2. Golgi apparatus Source: UniProtKB-SubCell
  3. membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 13061306Synergin gammaPRO_0000072388Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei471 – 4711PhosphoserineBy similarity
Modified residuei509 – 5091N6-acetyllysine1 Publication
Modified residuei576 – 5761Phosphoserine1 Publication
Modified residuei715 – 7151PhosphoserineBy similarity
Modified residuei736 – 7361N6-acetyllysineBy similarity
Modified residuei744 – 7441Phosphoserine1 Publication
Modified residuei844 – 8441PhosphoserineBy similarity
Modified residuei847 – 8471PhosphoserineBy similarity
Modified residuei901 – 9011PhosphoserineBy similarity
Modified residuei911 – 9111PhosphoserineBy similarity
Modified residuei927 – 9271PhosphoserineBy similarity
Modified residuei974 – 9741Phosphoserine2 Publications
Modified residuei1067 – 10671Phosphoserine1 Publication

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiQ5SV85.
PaxDbiQ5SV85.
PRIDEiQ5SV85.

PTM databases

PhosphoSiteiQ5SV85.

Expressioni

Gene expression databases

BgeeiQ5SV85.
CleanExiMM_AP1GBP1.
ExpressionAtlasiQ5SV85. baseline and differential.
GenevestigatoriQ5SV85.

Interactioni

Subunit structurei

Interacts with SCAMP1 via its EH-domain. Interacts with GGA1, GGA2 and GGA3. Interacts with the AP1G1 and AP1G2 subunits of the adapter protein complexes AP-1 (By similarity).By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000059000.

Structurei

3D structure databases

ProteinModelPortaliQ5SV85.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini293 – 404112EHPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni514 – 778265Interaction with A1P1G1 and A1P1G2By similarityAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili113 – 15341Sequence AnalysisAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi455 – 4595DFXDF motif 1
Motifi685 – 6895DFXDF motif 2
Motifi767 – 7715DFXDF motif 3

Domaini

The DFXDF motifs mediate the interaction with gamma-appendage subunits AP1G1 and AP1G2.By similarity

Sequence similaritiesi

Contains 1 EH domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Repeat

Phylogenomic databases

eggNOGiNOG136044.
GeneTreeiENSGT00390000010789.
HOGENOMiHOG000293152.
HOVERGENiHBG055053.
InParanoidiQ5SV85.
OrthoDBiEOG7GXP9S.
PhylomeDBiQ5SV85.
TreeFamiTF316700.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
InterProiIPR011992. EF-hand-dom_pair.
IPR000261. EPS15_homology.
[Graphical view]
PROSITEiPS50031. EH. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q5SV85-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MALRPGAGAS GAAGAGAGPG GAGSFMFPVA GGMRPPQAGL IPMQQQGFPM
60 70 80 90 100
VSVMQPNMQG MMGMNYSSQM SQGPIAMQAG IPMGPMPAAG VPFLGQPPFL
110 120 130 140 150
SMRPAGPQYT PDMQKQFAEE QQKRFEQQQK LLEEERKRRQ FEEQKQKLRL
160 170 180 190 200
LSSVKPKTGE KNRDDALEAI KGNLDGFSRD AKMHPTPASH PKKQGPSLEE
210 220 230 240 250
KLLVSCDVSA SGQEHIKLNT PDAGHKAIVP GSSKNCPGLM AHNRGAVDGC
260 270 280 290 300
VSGPASAEAE KTSDQTLSKE ESGVGVFPSQ DPAQSRMPPW IYNESLVPDA
310 320 330 340 350
YKKILETTMT PTGIDTAKLY PILMSSGLPR ETLGQIWALA NRTTPGRLTK
360 370 380 390 400
EELYTVLAMV AVTQRGVPAM SPDALSQFPA APIPTLSGFP MTLPTPVSQP
410 420 430 440 450
TAMPSGPTGS MPLTLGQPIM GINLVGPVGG AAAPTSSGFM PAYPSNQVGK
460 470 480 490 500
TEEDDFQDFQ DASKSGSIDD SFTDFQEMPA SSKTSNSQHG NSAPSLLIPF
510 520 530 540 550
PGTKASTDKY AVFKGISTDK PSENPASFGE SGDKYSAFRE LEQTTDSKPL
560 570 580 590 600
GESFAEFRST GTDDGFTDFK TADSVSPLEP PTKDTFPSAF ASGAAQQTQT
610 620 630 640 650
QVKTPLNLED LDMFSSVDCS GEKQVPFSAT FSTAKSVSTR PQPAGSAAAS
660 670 680 690 700
AALASTKTSS LADDFGEFNL FGEYSNPASA GEQDDFADFM AFGNSSISSE
710 720 730 740 750
PKASDKYEAL REEVSPSPLS SSTVEGAQHP PAAATKYDVF KQLSLEGAGL
760 770 780 790 800
AMEEFKENTS STKSEDDFAD FHSSKFSSTS SDKSLGEKAV AFRHAKEDSS
810 820 830 840 850
SVKSLDLPSI GGSSVGKEDS EDALSVQFDM KLADVGGDLK HVMSDSSLDL
860 870 880 890 900
PTVSGQHPPA ADTEDLSCAA FGSCSSHFTV STLTSCEWSD RADALQGRKL
910 920 930 940 950
SPFVLSAGSR SFSATSNLHT KEISFGSSEN ITMSSLSKGS ALASEDALPE
960 970 980 990 1000
TAFPAFASFK DMMPQTTEQK EFESGDFQDF TRQDMPTVDR SQETSCPSPA
1010 1020 1030 1040 1050
SSVASHETPK EGADDFGEFQ SEKSKISKFD FLVANSQSKM KSSEEMIKSE
1060 1070 1080 1090 1100
LATFDLSVQG SHKRSLSLGD KEISRSSPSP ALEQPFRDRS NTLSERAALP
1110 1120 1130 1140 1150
VIRDKYKDLT GEVEENERYA YEWQRCLGSA LDVIKKANDT LNGISSSAVC
1160 1170 1180 1190 1200
TEVIQSAQGM EYLLGVVEVY RVTKRVELGI KATAVCSEKL QQLLKDIDKV
1210 1220 1230 1240 1250
WNNLIGFMSL ATLTPDENSL DFSSCMLRPG IKNAQELACG VCLLNVDSRS
1260 1270 1280 1290 1300
RKEETPAEEQ PKKAFNSETD SFKLAYGGHQ YHASCANFWI NCVEPKPPGL

LLPDLL
Length:1,306
Mass (Da):139,616
Last modified:December 21, 2004 - v1
Checksum:iEB149A0B7ADAE2D7
GO
Isoform 2 (identifier: Q5SV85-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     38-38: Missing.
     195-272: Missing.
     862-938: Missing.
     1252-1263: Missing.

Show »
Length:1,138
Mass (Da):122,083
Checksum:i2FDF97B632587B8E
GO

Sequence cautioni

The sequence CAI25511.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei38 – 381Missing in isoform 2. 1 PublicationVSP_013251
Alternative sequencei195 – 27278Missing in isoform 2. 1 PublicationVSP_013252Add
BLAST
Alternative sequencei862 – 93877Missing in isoform 2. 1 PublicationVSP_013253Add
BLAST
Alternative sequencei1252 – 126312Missing in isoform 2. 1 PublicationVSP_013254Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL645615 Genomic DNA. Translation: CAI25509.1.
AL645615 Genomic DNA. Translation: CAI25510.1.
AL645615 Genomic DNA. Translation: CAI25511.1. Sequence problems.
BC056370 mRNA. Translation: AAH56370.1.
CCDSiCCDS25181.1. [Q5SV85-2]
RefSeqiNP_919322.1. NM_194341.2. [Q5SV85-2]
UniGeneiMm.441722.
Mm.82680.

Genome annotation databases

EnsembliENSMUST00000049714; ENSMUSP00000059000; ENSMUSG00000034940. [Q5SV85-1]
ENSMUST00000092834; ENSMUSP00000090510; ENSMUSG00000034940. [Q5SV85-2]
GeneIDi217030.
KEGGimmu:217030.
UCSCiuc007kqa.2. mouse. [Q5SV85-2]
uc011ybj.1. mouse. [Q5SV85-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL645615 Genomic DNA. Translation: CAI25509.1.
AL645615 Genomic DNA. Translation: CAI25510.1.
AL645615 Genomic DNA. Translation: CAI25511.1. Sequence problems.
BC056370 mRNA. Translation: AAH56370.1.
CCDSiCCDS25181.1. [Q5SV85-2]
RefSeqiNP_919322.1. NM_194341.2. [Q5SV85-2]
UniGeneiMm.441722.
Mm.82680.

3D structure databases

ProteinModelPortaliQ5SV85.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000059000.

PTM databases

PhosphoSiteiQ5SV85.

Proteomic databases

MaxQBiQ5SV85.
PaxDbiQ5SV85.
PRIDEiQ5SV85.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000049714; ENSMUSP00000059000; ENSMUSG00000034940. [Q5SV85-1]
ENSMUST00000092834; ENSMUSP00000090510; ENSMUSG00000034940. [Q5SV85-2]
GeneIDi217030.
KEGGimmu:217030.
UCSCiuc007kqa.2. mouse. [Q5SV85-2]
uc011ybj.1. mouse. [Q5SV85-1]

Organism-specific databases

CTDi11276.
MGIiMGI:1354742. Synrg.

Phylogenomic databases

eggNOGiNOG136044.
GeneTreeiENSGT00390000010789.
HOGENOMiHOG000293152.
HOVERGENiHBG055053.
InParanoidiQ5SV85.
OrthoDBiEOG7GXP9S.
PhylomeDBiQ5SV85.
TreeFamiTF316700.

Miscellaneous databases

NextBioi375518.
PROiQ5SV85.
SOURCEiSearch...

Gene expression databases

BgeeiQ5SV85.
CleanExiMM_AP1GBP1.
ExpressionAtlasiQ5SV85. baseline and differential.
GenevestigatoriQ5SV85.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
InterProiIPR011992. EF-hand-dom_pair.
IPR000261. EPS15_homology.
[Graphical view]
PROSITEiPS50031. EH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Strain: C57BL/6.
    Tissue: Brain.
  3. "Binding partners for the COOH-terminal appendage domains of the GGAs and gamma-adaptin."
    Lui W.W.Y., Collins B.M., Hirst J., Motley A., Millar C., Schu P., Owen D.J., Robinson M.S.
    Mol. Biol. Cell 14:2385-2398(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  4. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-974, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  5. "The phagosomal proteome in interferon-gamma-activated macrophages."
    Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
    Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-576; SER-744; SER-974 AND SER-1067, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  6. "SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
    Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
    Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-509, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic fibroblast.

Entry informationi

Entry nameiSYNRG_MOUSE
AccessioniPrimary (citable) accession number: Q5SV85
Secondary accession number(s): Q5SV84, Q6PHT6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 29, 2005
Last sequence update: December 21, 2004
Last modified: January 7, 2015
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.