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Q5SV85 (SYNRG_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 85. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Synergin gamma
Alternative name(s):
AP1 subunit gamma-binding protein 1
Gamma-synergin
Gene names
Name:Synrg
Synonyms:Ap1gbp1, Syng
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length1306 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

May play a role in endocytosis and/or membrane trafficking at the trans-Golgi network (TGN). May act by linking the adapter protein complex AP-1 to other proteins By similarity.

Subunit structure

Interacts with SCAMP1 via its EH-domain. Interacts with GGA1, GGA2 and GGA3. Interacts with the AP1G1 and AP1G2 subunits of the adapter protein complexes AP-1 By similarity.

Subcellular location

Cytoplasmcytosol. Golgi apparatustrans-Golgi network membrane; Peripheral membrane protein. Note: Associated with membranes of the TGN, colocalizes with AP1G1 By similarity. Associates with membranes via the adapter protein complex AP-1. Ref.3

Domain

The DFXDF motifs mediate the interaction with gamma-appendage subunits AP1G1 and AP1G2 By similarity.

Sequence similarities

Contains 1 EH domain.

Sequence caution

The sequence CAI25511.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
   Biological processEndocytosis
Protein transport
Transport
   Cellular componentCytoplasm
Golgi apparatus
Membrane
   Coding sequence diversityAlternative splicing
   DomainCoiled coil
Repeat
   PTMAcetylation
Phosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processendocytosis

Inferred from electronic annotation. Source: UniProtKB-KW

protein transport

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentGolgi apparatus

Inferred from electronic annotation. Source: UniProtKB-SubCell

cytosol

Inferred from electronic annotation. Source: UniProtKB-SubCell

membrane

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functioncalcium ion binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q5SV85-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q5SV85-2)

The sequence of this isoform differs from the canonical sequence as follows:
     38-38: Missing.
     195-272: Missing.
     862-938: Missing.
     1252-1263: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 13061306Synergin gamma
PRO_0000072388

Regions

Domain293 – 404112EH
Region514 – 778265Interaction with A1P1G1 and A1P1G2 By similarity
Coiled coil113 – 15341 Potential
Motif455 – 4595DFXDF motif 1
Motif685 – 6895DFXDF motif 2
Motif767 – 7715DFXDF motif 3

Amino acid modifications

Modified residue4711Phosphoserine By similarity
Modified residue5091N6-acetyllysine Ref.6
Modified residue5761Phosphoserine Ref.5
Modified residue7151Phosphoserine By similarity
Modified residue7361N6-acetyllysine By similarity
Modified residue7441Phosphoserine Ref.5
Modified residue8441Phosphoserine By similarity
Modified residue8471Phosphoserine By similarity
Modified residue9011Phosphoserine By similarity
Modified residue9111Phosphoserine By similarity
Modified residue9271Phosphoserine By similarity
Modified residue9741Phosphoserine Ref.4 Ref.5
Modified residue10671Phosphoserine Ref.5

Natural variations

Alternative sequence381Missing in isoform 2.
VSP_013251
Alternative sequence195 – 27278Missing in isoform 2.
VSP_013252
Alternative sequence862 – 93877Missing in isoform 2.
VSP_013253
Alternative sequence1252 – 126312Missing in isoform 2.
VSP_013254

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified December 21, 2004. Version 1.
Checksum: EB149A0B7ADAE2D7

FASTA1,306139,616
        10         20         30         40         50         60 
MALRPGAGAS GAAGAGAGPG GAGSFMFPVA GGMRPPQAGL IPMQQQGFPM VSVMQPNMQG 

        70         80         90        100        110        120 
MMGMNYSSQM SQGPIAMQAG IPMGPMPAAG VPFLGQPPFL SMRPAGPQYT PDMQKQFAEE 

       130        140        150        160        170        180 
QQKRFEQQQK LLEEERKRRQ FEEQKQKLRL LSSVKPKTGE KNRDDALEAI KGNLDGFSRD 

       190        200        210        220        230        240 
AKMHPTPASH PKKQGPSLEE KLLVSCDVSA SGQEHIKLNT PDAGHKAIVP GSSKNCPGLM 

       250        260        270        280        290        300 
AHNRGAVDGC VSGPASAEAE KTSDQTLSKE ESGVGVFPSQ DPAQSRMPPW IYNESLVPDA 

       310        320        330        340        350        360 
YKKILETTMT PTGIDTAKLY PILMSSGLPR ETLGQIWALA NRTTPGRLTK EELYTVLAMV 

       370        380        390        400        410        420 
AVTQRGVPAM SPDALSQFPA APIPTLSGFP MTLPTPVSQP TAMPSGPTGS MPLTLGQPIM 

       430        440        450        460        470        480 
GINLVGPVGG AAAPTSSGFM PAYPSNQVGK TEEDDFQDFQ DASKSGSIDD SFTDFQEMPA 

       490        500        510        520        530        540 
SSKTSNSQHG NSAPSLLIPF PGTKASTDKY AVFKGISTDK PSENPASFGE SGDKYSAFRE 

       550        560        570        580        590        600 
LEQTTDSKPL GESFAEFRST GTDDGFTDFK TADSVSPLEP PTKDTFPSAF ASGAAQQTQT 

       610        620        630        640        650        660 
QVKTPLNLED LDMFSSVDCS GEKQVPFSAT FSTAKSVSTR PQPAGSAAAS AALASTKTSS 

       670        680        690        700        710        720 
LADDFGEFNL FGEYSNPASA GEQDDFADFM AFGNSSISSE PKASDKYEAL REEVSPSPLS 

       730        740        750        760        770        780 
SSTVEGAQHP PAAATKYDVF KQLSLEGAGL AMEEFKENTS STKSEDDFAD FHSSKFSSTS 

       790        800        810        820        830        840 
SDKSLGEKAV AFRHAKEDSS SVKSLDLPSI GGSSVGKEDS EDALSVQFDM KLADVGGDLK 

       850        860        870        880        890        900 
HVMSDSSLDL PTVSGQHPPA ADTEDLSCAA FGSCSSHFTV STLTSCEWSD RADALQGRKL 

       910        920        930        940        950        960 
SPFVLSAGSR SFSATSNLHT KEISFGSSEN ITMSSLSKGS ALASEDALPE TAFPAFASFK 

       970        980        990       1000       1010       1020 
DMMPQTTEQK EFESGDFQDF TRQDMPTVDR SQETSCPSPA SSVASHETPK EGADDFGEFQ 

      1030       1040       1050       1060       1070       1080 
SEKSKISKFD FLVANSQSKM KSSEEMIKSE LATFDLSVQG SHKRSLSLGD KEISRSSPSP 

      1090       1100       1110       1120       1130       1140 
ALEQPFRDRS NTLSERAALP VIRDKYKDLT GEVEENERYA YEWQRCLGSA LDVIKKANDT 

      1150       1160       1170       1180       1190       1200 
LNGISSSAVC TEVIQSAQGM EYLLGVVEVY RVTKRVELGI KATAVCSEKL QQLLKDIDKV 

      1210       1220       1230       1240       1250       1260 
WNNLIGFMSL ATLTPDENSL DFSSCMLRPG IKNAQELACG VCLLNVDSRS RKEETPAEEQ 

      1270       1280       1290       1300 
PKKAFNSETD SFKLAYGGHQ YHASCANFWI NCVEPKPPGL LLPDLL 

« Hide

Isoform 2 [UniParc].

Checksum: 2FDF97B632587B8E
Show »

FASTA1,138122,083

References

« Hide 'large scale' references
[1]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Strain: C57BL/6.
Tissue: Brain.
[3]"Binding partners for the COOH-terminal appendage domains of the GGAs and gamma-adaptin."
Lui W.W.Y., Collins B.M., Hirst J., Motley A., Millar C., Schu P., Owen D.J., Robinson M.S.
Mol. Biol. Cell 14:2385-2398(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
[4]"Large-scale phosphorylation analysis of mouse liver."
Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-974, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Liver.
[5]"The phagosomal proteome in interferon-gamma-activated macrophages."
Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-576; SER-744; SER-974 AND SER-1067, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[6]"SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-509, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Embryonic fibroblast.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AL645615 Genomic DNA. Translation: CAI25509.1.
AL645615 Genomic DNA. Translation: CAI25510.1.
AL645615 Genomic DNA. Translation: CAI25511.1. Sequence problems.
BC056370 mRNA. Translation: AAH56370.1.
CCDSCCDS25181.1. [Q5SV85-2]
RefSeqNP_919322.1. NM_194341.2. [Q5SV85-2]
UniGeneMm.441722.
Mm.82680.

3D structure databases

ProteinModelPortalQ5SV85.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10090.ENSMUSP00000059000.

PTM databases

PhosphoSiteQ5SV85.

Proteomic databases

MaxQBQ5SV85.
PaxDbQ5SV85.
PRIDEQ5SV85.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000049714; ENSMUSP00000059000; ENSMUSG00000034940. [Q5SV85-1]
ENSMUST00000092834; ENSMUSP00000090510; ENSMUSG00000034940. [Q5SV85-2]
GeneID217030.
KEGGmmu:217030.
UCSCuc007kqa.2. mouse. [Q5SV85-2]
uc011ybj.1. mouse. [Q5SV85-1]

Organism-specific databases

CTD11276.
MGIMGI:1354742. Synrg.

Phylogenomic databases

eggNOGNOG136044.
GeneTreeENSGT00390000010789.
HOGENOMHOG000293152.
HOVERGENHBG055053.
InParanoidQ5SV85.
OMADLDMFSS.
OrthoDBEOG7GXP9S.
PhylomeDBQ5SV85.
TreeFamTF316700.

Gene expression databases

BgeeQ5SV85.
CleanExMM_AP1GBP1.
GenevestigatorQ5SV85.

Family and domain databases

Gene3D1.10.238.10. 1 hit.
InterProIPR011992. EF-hand-dom_pair.
IPR000261. EPS15_homology.
[Graphical view]
PROSITEPS50031. EH. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio375518.
PROQ5SV85.
SOURCESearch...

Entry information

Entry nameSYNRG_MOUSE
AccessionPrimary (citable) accession number: Q5SV85
Secondary accession number(s): Q5SV84, Q6PHT6
Entry history
Integrated into UniProtKB/Swiss-Prot: March 29, 2005
Last sequence update: December 21, 2004
Last modified: July 9, 2014
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot