Q5SV85 (SYNRG_MOUSE) Reviewed, UniProtKB/Swiss-Prot
Last modified
May 1, 2013.
Version 74.
History...
Clusters with 
Names·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: Synergin gamma Alternative name(s): AP1 subunit gamma-binding protein 1 Gamma-synergin | ||||
| Gene names |
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| Organism | Mus musculus (Mouse) [Reference proteome] | ||||
| Taxonomic identifier | 10090 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus |
Protein attributes
| Sequence length | 1306 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at protein level |
General annotation (Comments)
| Function | May play a role in endocytosis and/or membrane trafficking at the trans-Golgi network (TGN). May act by linking the adapter protein complex AP-1 to other proteins By similarity. |
| Subunit structure | Interacts with SCAMP1 via its EH-domain. Interacts with GGA1, GGA2 and GGA3. Interacts with the AP1G1 and AP1G2 subunits of the adapter protein complexes AP-1 By similarity. |
| Subcellular location | Cytoplasm › cytosol. Golgi apparatus › trans-Golgi network membrane; Peripheral membrane protein. Note: Associated with membranes of the TGN, colocalizes with AP1G1 By similarity. Associates with membranes via the adapter protein complex AP-1. Ref.3 |
| Domain | The DFXDF motifs mediate the interaction with gamma-appendage subunits AP1G1 and AP1G2 By similarity. |
| Sequence similarities | Contains 1 EH domain. |
| Sequence caution | The sequence CAI25511.1 differs from that shown. Reason: Erroneous gene model prediction. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Endocytosis Protein transport Transport |
| Cellular component | Cytoplasm Golgi apparatus Membrane |
| Coding sequence diversity | Alternative splicing |
| Domain | Coiled coil Repeat |
| PTM | Acetylation Phosphoprotein |
| Technical term | Complete proteome Reference proteome |
| Gene Ontology (GO) | |
| Biological_process | endocytosis Inferred from electronic annotation. Source: UniProtKB-KW protein transportInferred from electronic annotation. Source: UniProtKB-KW |
| Cellular_component | Golgi apparatus Inferred from electronic annotation. Source: UniProtKB-SubCell cytosolInferred from electronic annotation. Source: UniProtKB-SubCell membraneInferred from electronic annotation. Source: UniProtKB-KW |
| Molecular_function | calcium ion binding Inferred from electronic annotation. Source: InterPro |
| Complete GO annotation... | |
Alternative products
| This entry describes 2 isoforms produced by alternative splicing. [Align] [Select] | ||||||
| Isoform 1 (identifier: Q5SV85-1) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. | ||||||
| Isoform 2 (identifier: Q5SV85-2) The sequence of this isoform differs from the canonical sequence as follows: 38-38: Missing. 195-272: Missing. 862-938: Missing. 1252-1263: Missing. |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 1306 | 1306 | Synergin gamma | PRO_0000072388 | |||||
Regions | |||||||||
| Domain | 293 – 404 | 112 | EH | ||||||
| Region | 514 – 778 | 265 | Interaction with A1P1G1 and A1P1G2 By similarity | ||||||
| Coiled coil | 113 – 153 | 41 | Potential | ||||||
| Motif | 455 – 459 | 5 | DFXDF motif 1 | ||||||
| Motif | 685 – 689 | 5 | DFXDF motif 2 | ||||||
| Motif | 767 – 771 | 5 | DFXDF motif 3 | ||||||
Amino acid modifications | |||||||||
| Modified residue | 471 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 509 | 1 | N6-acetyllysine By similarity | ||||||
| Modified residue | 715 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 736 | 1 | N6-acetyllysine By similarity | ||||||
| Modified residue | 744 | 1 | Phosphoserine Ref.5 | ||||||
| Modified residue | 844 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 847 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 901 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 911 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 927 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 974 | 1 | Phosphoserine Ref.4 | ||||||
| Modified residue | 1067 | 1 | Phosphoserine By similarity | ||||||
Natural variations | |||||||||
| Alternative sequence | 38 | 1 | Missing in isoform 2. | VSP_013251 | |||||
| Alternative sequence | 195 – 272 | 78 | Missing in isoform 2. | VSP_013252 | |||||
| Alternative sequence | 862 – 938 | 77 | Missing in isoform 2. | VSP_013253 | |||||
| Alternative sequence | 1252 – 1263 | 12 | Missing in isoform 2. | VSP_013254 | |||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.  Ponting C.P.PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6J. |
| [2] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Strain: C57BL/6. Tissue: Brain. |
| [3] | "Binding partners for the COOH-terminal appendage domains of the GGAs and gamma-adaptin." Lui W.W.Y., Collins B.M., Hirst J., Motley A., Millar C., Schu P., Owen D.J., Robinson M.S. Mol. Biol. Cell 14:2385-2398(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION. |
| [4] | "Protein phosphorylation and expression profiling by Yin-yang multidimensional liquid chromatography (Yin-yang MDLC) mass spectrometry." Dai J., Jin W.-H., Sheng Q.-H., Shieh C.-H., Wu J.-R., Zeng R. J. Proteome Res. 6:250-262(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-974, MASS SPECTROMETRY. Tissue: Liver. |
| [5] | "The phagosomal proteome in interferon-gamma-activated macrophages." Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P. Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-744, MASS SPECTROMETRY. Tissue: Macrophage. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | AL645615 Genomic DNA. Translation: CAI25509.1. AL645615 Genomic DNA. Translation: CAI25510.1. AL645615 Genomic DNA. Translation: CAI25511.1. Sequence problems. BC056370 mRNA. Translation: AAH56370.1. |
| IPI | IPI00380008. IPI00515622. |
| RefSeq | NP_001108481.1. NM_001115009.1. NP_919322.1. NM_194341.2. |
| UniGene | Mm.82680. |
3D structure databases | |
| ProteinModelPortal | Q5SV85. |
| SMR | Q5SV85. Positions 298-376. |
| ModBase | Search... |
Protein-protein interaction databases | |
| STRING | 10090.ENSMUSP00000059000. |
PTM databases | |
| PhosphoSite | Q5SV85. |
Proteomic databases | |
| PaxDb | Q5SV85. |
| PRIDE | Q5SV85. |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| Ensembl | ENSMUST00000049714; ENSMUSP00000059000; ENSMUSG00000034940. ENSMUST00000092834; ENSMUSP00000090510; ENSMUSG00000034940. |
| GeneID | 217030. |
| KEGG | mmu:217030. |
| UCSC | uc007kqa.2. mouse. uc011ybj.1. mouse. |
Organism-specific databases | |
| CTD | 11276. |
| MGI | MGI:1354742. Synrg. |
Phylogenomic databases | |
| eggNOG | NOG136044. |
| GeneTree | ENSGT00390000010789. |
| HOGENOM | HOG000293152. |
| HOVERGEN | HBG055053. |
| InParanoid | Q5SV85. |
| OMA | DLDMFSS. |
| OrthoDB | EOG4P5K8D. |
Gene expression databases | |
| Bgee | Q5SV85. |
| CleanEx | MM_AP1GBP1. |
| Genevestigator | Q5SV85. |
| GermOnline | ENSMUSG00000034940. Mus musculus. |
Family and domain databases | |
| Gene3D | 1.10.238.10. 1 hit. |
| InterPro | IPR011992. EF-hand-like_dom. IPR000261. EPS15_homology. [Graphical view] |
| PROSITE | PS50031. EH. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other | |
| NextBio | 375518. |
| SOURCE | Search... |
Entry information
| Entry name | SYNRG_MOUSE | ||||||||
| Accession | Primary (citable) accession number: Q5SV85 Secondary accession number(s): Q5SV84, Q6PHT6 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Chordata Protein Annotation Program | ||||||||
Relevant documents
| MGD cross-references Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot |
| SIMILARITY comments Index of protein domains and families |