Reviewed, UniProtKB/Swiss-Prot Q5SUR0 (PUR4_MOUSE)

Names and origin

Protein names

Recommended name:
    Phosphoribosylformylglycinamidine synthase
      Short name=FGAM synthase
      Short name=FGAMS
    EC=6.3.5.3
Alternative name(s):
    Formylglycinamide ribotide amidotransferase
      Short name=FGARAT
    Formylglycinamide ribotide synthetase

Gene names

Name:	Pfas
Synonyms:	Kiaa0361

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	1337 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	ATP + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O = ADP + phosphate + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine + L-glutamate.
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/2.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	In the N-terminal section; belongs to the FGAMS family. Contains 1 glutamine amidotransferase type-1 domain.

Ontologies

Keywords
Biological process	Purine biosynthesis
Cellular component	Cytoplasm
Domain	Glutamine amidotransferase
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Gene Ontology (GO)
Biological process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: InterPro glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoribosylformylglycinamidine synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1337	1337	Phosphoribosylformylglycinamidine synthase		PRO_0000370848
Regions
	Domain	1063 – 1301	239	Glutamine amidotransferase type-1
	Nucleotide binding	322 – 333	12	ATP Potential
Sites
	Active site	1157	1	For GATase activity By similarity
Experimental info
	Sequence conflict	306	1	F → L in AAI14998. Ref.2
	Sequence conflict	594 – 597	4	GDKR → WGPQ in BAD32216. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Q5SUR0-1 [UniParc]. Last modified December 21, 2004. Version 1. Checksum: DC0DD64F17F80030	FASTA	1,337	144,629
	10 20 30 40 50 60 MAPVLHFYVR PSGHEGAASG RVFRRLQEKL PTLQSVETEL CYNVHWAAET LPWAEEMKKL 70 80 90 100 110 120 MWLFGCPLVR DDVAQEPWLV PGSNDLLLEV GPRLNFSTPA STNIVSVCQA AGLRAVDRVE 130 140 150 160 170 180 TTRRYRLSFT DHPTAEMEAI SLAALHDRMT EQHYPDPIQS FSPQSIPAPL KGSIDILAEG 190 200 210 220 230 240 RPALEKANQE LGLALDSWDL DFYTKRFQEL QRNPSTVEVF DLAQSNSEHS RHWFFKGQLH 250 260 270 280 290 300 VDGKKLAHSL FESIMSTQAS SNPNNVLKFC DNSSAIQGKK VKFLRPEDST RPSCFQQQQG 310 320 330 340 350 360 LRHVVFTAET HNFPTGVAPF SGATTGTGGR IRDVQCTGRG AHVVAGTAGY CFGNLHIPDY 370 380 390 400 410 420 NLPWEDPSFQ YPGNFARPLE VAIEASNGAS DYGNKFGEPV LAGFARSLGL QLPDGQRREW 430 440 450 460 470 480 IKPIMFSGGI GSMEAKHVGK KPPEPGMEVV KVGGPVYRIG VGGGAASSVQ VQGDNTSDLD 490 500 510 520 530 540 FGAVQRGDPE MEQKMNRVIR ACVEAPGGNP ICSLHDQGAG GNGNVLKELS DPEGAIIYTS 550 560 570 580 590 600 RFQLGDPTLN ALEIWGAEYQ ESNALLLRPS DRDFLSRASA RERCPACFVG TITGDKRIVL 610 620 630 640 650 660 VDDRECLVGK TGQGDAPLTP PTPVDLDLDW VLGKMPQKEF FLQRKPPVLQ PLALPPELSV 670 680 690 700 710 720 RQALNRVLRL PAVASKRYLT NKVDRSVGGL VAQQQCVGPL QTPLADVAVV ALSHQECIGA 730 740 750 760 770 780 ATALGEQPVK SLLDPKAAAR LAVSEALTNL VFALVTDLRD VKCSGNWMWA AKLPGEGAAL 790 800 810 820 830 840 ADACEAMVAV MAALGVAVDG GKDSLSMAAR VGTETVQAPG SLVISAYAVC PDITATVTPD 850 860 870 880 890 900 LKHPGGKGHL LYVPLSPGQH RLGGTALAQC FSQLGEHPPD LDLPENLVRA FHITQGLLKE 910 920 930 940 950 960 CRLCSGHDVS DGGLVTCLLE MAFAGNCGIE VDVPAPGIHA LPVLFAEEPG LVLEVQEADV 970 980 990 1000 1010 1020 AGVRQRYESA GLRCLELGHT GEAGPQAMAR ISVNKAVVVE EPVGELRALW EETSFQLDLL 1030 1040 1050 1060 1070 1080 QAEPRCVIEE KQGLKERTGP SYYLPPTFPV ASVPCKPGGP VPRVAILREE GSNGDREMAD 1090 1100 1110 1120 1130 1140 AFHLAGFEVW DVTMQDLCSG AIRLDTFRGV AFVGGFSYAD VLGSAKGWAA AVTFNPQARE 1150 1160 1170 1180 1190 1200 ELGRFRRRPD TFSLGVCNGC QLLALLGWVG SDPSEEQAEP GQDSQPTQPG LLLRHNLSGR 1210 1220 1230 1240 1250 1260 FESRWATVRV EPGPALMLRG MEGSVLPVWS AHGEGYMAFS SPELQAKIEA KGLVPLHWAD 1270 1280 1290 1300 1310 1320 DDGNPTEQYP LNPNGSPGGI AGICSQDGRH LALMPHPERA VRLWQWAWRP SPFDVLPTSP 1330 WLQLFINARN WTQEDSC

References

[1]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed: 19468303] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6J.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J.
[3]	"Prediction of the coding sequences of mouse homologues of KIAA gene: IV. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries." Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S., Saga Y., Seino S., Nishimura M., Kaisho T., Hoshino K., Kitamura H., Nagase T., Ohara O., Koga H. DNA Res. 11:205-218(2004) [PubMed: 15368895] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 594-1337. Tissue: Pancreatic islet.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AL645902 Genomic DNA. Translation: CAI24440.1. BC114997 mRNA. Translation: AAI14998.1. AK172938 mRNA. Translation: BAD32216.1.
IPI	IPI00265406.
RefSeq	NP_001152991.1.
UniGene	Mm.340288
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q5SUR0.
PTM databases
PhosphoSite	Q5SUR0.
Proteomic databases
PRIDE	Q5SUR0.
Genome annotation databases
Ensembl	ENSMUST00000021282; ENSMUSP00000021282; ENSMUSG00000020899; Mus musculus. [Genome view] ENSMUST00000108668; ENSMUSP00000104308; ENSMUSG00000020899; Mus musculus. [Genome view]
GeneID	237823.
KEGG	mmu:237823.
UCSC	uc007jot.1. mouse. uc007jou.1. mouse.
Organism-specific databases
CTD	237823.
MGI	MGI:2684864. Pfas.
Rouge	Search...
Phylogenomic databases
HOGENOM	Q5SUR0.
HOVERGEN	Q5SUR0.
OMA	NLHIPDY.
Gene expression databases
ArrayExpress	Q5SUR0.
Bgee	Q5SUR0.
Genevestigator	Q5SUR0.
Family and domain databases
InterPro	IPR000728. AIR_synth. IPR010918. AIR_synth_C. IPR017926. GATASE_1. IPR010073. PRibForGlyAmidine_synth. [Graphical view]
Pfam	PF00586. AIRS. 1 hit. PF02769. AIRS_C. 2 hits. [Graphical view]
TIGRFAMs	TIGR01735. FGAM_synt. 1 hit.
PROSITE	PS51273. GATASE_TYPE_1. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	383508.
SOURCE	Search...

Entry information

Entry name

PUR4_MOUSE

Accession

Primary (citable) accession number: Q5SUR0
Secondary accession number(s): A4FUJ6, Q6A080

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 5, 2009
Last sequence update:	December 21, 2004
Last modified:	November 3, 2009
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)