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Protein

Phosphoribosylformylglycinamidine synthase

Gene

Pfas

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Phosphoribosylformylglycinamidine synthase involved in the purines biosynthetic pathway. Catalyzes the ATP-dependent conversion of formylglycinamide ribonucleotide (FGAR) and glutamine to yield formylglycinamidine ribonucleotide (FGAM) and glutamate (By similarity).By similarity

Catalytic activityi

ATP + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O = ADP + phosphate + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine + L-glutamate.

Pathwayi: IMP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Phosphoribosylformylglycinamidine synthase (Pfas)
  2. Trifunctional purine biosynthetic protein adenosine-3 (Gart)
This subpathway is part of the pathway IMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide, the pathway IMP biosynthesis via de novo pathway and in Purine metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei705 – 7051ATP; via carbonyl oxygenBy similarity
Metal bindingi706 – 7061MagnesiumBy similarity
Metal bindingi745 – 7451MagnesiumBy similarity
Metal bindingi749 – 7491MagnesiumBy similarity
Metal bindingi908 – 9081MagnesiumBy similarity
Binding sitei910 – 9101ATPBy similarity
Active sitei1157 – 11571NucleophileBy similarity
Active sitei1296 – 12961By similarity
Active sitei1298 – 12981By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi322 – 33312ATPSequence analysisAdd
BLAST
Nucleotide bindingi402 – 4043ATPBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Purine biosynthesis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-73817. Purine ribonucleoside monophosphate biosynthesis.
UniPathwayiUPA00074; UER00128.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoribosylformylglycinamidine synthase (EC:6.3.5.3)
Short name:
FGAM synthase
Short name:
FGAMS
Alternative name(s):
Formylglycinamide ribonucleotide amidotransferase
Short name:
FGAR amidotransferase
Short name:
FGAR-AT
Formylglycinamide ribotide amidotransferase
Gene namesi
Name:Pfas
Synonyms:Kiaa0361
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:2684864. Pfas.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 13371337Phosphoribosylformylglycinamidine synthasePRO_0000370848Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei215 – 2151PhosphoserineBy similarity
Modified residuei619 – 6191PhosphothreonineBy similarity
Modified residuei622 – 6221PhosphothreonineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ5SUR0.
MaxQBiQ5SUR0.
PaxDbiQ5SUR0.
PeptideAtlasiQ5SUR0.
PRIDEiQ5SUR0.

PTM databases

iPTMnetiQ5SUR0.
PhosphoSiteiQ5SUR0.

Expressioni

Gene expression databases

BgeeiQ5SUR0.
ExpressionAtlasiQ5SUR0. baseline and differential.
GenevisibleiQ5SUR0. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000021282.

Structurei

3D structure databases

ProteinModelPortaliQ5SUR0.
SMRiQ5SUR0. Positions 304-569, 1062-1301.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1063 – 1301239Glutamine amidotransferase type-1Add
BLAST

Sequence similaritiesi

In the N-terminal section; belongs to the FGAMS family.Curated

Keywords - Domaini

Glutamine amidotransferase

Phylogenomic databases

eggNOGiKOG1907. Eukaryota.
COG0046. LUCA.
COG0047. LUCA.
GeneTreeiENSGT00390000007600.
HOGENOMiHOG000261358.
InParanoidiQ5SUR0.
KOiK01952.
OMAiECLENIC.
OrthoDBiEOG7353X4.
PhylomeDBiQ5SUR0.
TreeFamiTF106371.

Family and domain databases

Gene3Di3.30.1330.10. 2 hits.
3.40.50.880. 1 hit.
HAMAPiMF_00419. PurL_1.
InterProiIPR010918. AIR_synth_C_dom.
IPR029062. Class_I_gatase-like.
IPR017926. GATASE.
IPR010073. PRibForGlyAmidine_synth.
IPR016188. PurM-like_N.
[Graphical view]
PfamiPF02769. AIRS_C. 2 hits.
[Graphical view]
SUPFAMiSSF52317. SSF52317. 1 hit.
SSF56042. SSF56042. 2 hits.
TIGRFAMsiTIGR01735. FGAM_synt. 1 hit.
PROSITEiPS51273. GATASE_TYPE_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5SUR0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAPVLHFYVR PSGHEGAASG RVFRRLQEKL PTLQSVETEL CYNVHWAAET
60 70 80 90 100
LPWAEEMKKL MWLFGCPLVR DDVAQEPWLV PGSNDLLLEV GPRLNFSTPA
110 120 130 140 150
STNIVSVCQA AGLRAVDRVE TTRRYRLSFT DHPTAEMEAI SLAALHDRMT
160 170 180 190 200
EQHYPDPIQS FSPQSIPAPL KGSIDILAEG RPALEKANQE LGLALDSWDL
210 220 230 240 250
DFYTKRFQEL QRNPSTVEVF DLAQSNSEHS RHWFFKGQLH VDGKKLAHSL
260 270 280 290 300
FESIMSTQAS SNPNNVLKFC DNSSAIQGKK VKFLRPEDST RPSCFQQQQG
310 320 330 340 350
LRHVVFTAET HNFPTGVAPF SGATTGTGGR IRDVQCTGRG AHVVAGTAGY
360 370 380 390 400
CFGNLHIPDY NLPWEDPSFQ YPGNFARPLE VAIEASNGAS DYGNKFGEPV
410 420 430 440 450
LAGFARSLGL QLPDGQRREW IKPIMFSGGI GSMEAKHVGK KPPEPGMEVV
460 470 480 490 500
KVGGPVYRIG VGGGAASSVQ VQGDNTSDLD FGAVQRGDPE MEQKMNRVIR
510 520 530 540 550
ACVEAPGGNP ICSLHDQGAG GNGNVLKELS DPEGAIIYTS RFQLGDPTLN
560 570 580 590 600
ALEIWGAEYQ ESNALLLRPS DRDFLSRASA RERCPACFVG TITGDKRIVL
610 620 630 640 650
VDDRECLVGK TGQGDAPLTP PTPVDLDLDW VLGKMPQKEF FLQRKPPVLQ
660 670 680 690 700
PLALPPELSV RQALNRVLRL PAVASKRYLT NKVDRSVGGL VAQQQCVGPL
710 720 730 740 750
QTPLADVAVV ALSHQECIGA ATALGEQPVK SLLDPKAAAR LAVSEALTNL
760 770 780 790 800
VFALVTDLRD VKCSGNWMWA AKLPGEGAAL ADACEAMVAV MAALGVAVDG
810 820 830 840 850
GKDSLSMAAR VGTETVQAPG SLVISAYAVC PDITATVTPD LKHPGGKGHL
860 870 880 890 900
LYVPLSPGQH RLGGTALAQC FSQLGEHPPD LDLPENLVRA FHITQGLLKE
910 920 930 940 950
CRLCSGHDVS DGGLVTCLLE MAFAGNCGIE VDVPAPGIHA LPVLFAEEPG
960 970 980 990 1000
LVLEVQEADV AGVRQRYESA GLRCLELGHT GEAGPQAMAR ISVNKAVVVE
1010 1020 1030 1040 1050
EPVGELRALW EETSFQLDLL QAEPRCVIEE KQGLKERTGP SYYLPPTFPV
1060 1070 1080 1090 1100
ASVPCKPGGP VPRVAILREE GSNGDREMAD AFHLAGFEVW DVTMQDLCSG
1110 1120 1130 1140 1150
AIRLDTFRGV AFVGGFSYAD VLGSAKGWAA AVTFNPQARE ELGRFRRRPD
1160 1170 1180 1190 1200
TFSLGVCNGC QLLALLGWVG SDPSEEQAEP GQDSQPTQPG LLLRHNLSGR
1210 1220 1230 1240 1250
FESRWATVRV EPGPALMLRG MEGSVLPVWS AHGEGYMAFS SPELQAKIEA
1260 1270 1280 1290 1300
KGLVPLHWAD DDGNPTEQYP LNPNGSPGGI AGICSQDGRH LALMPHPERA
1310 1320 1330
VRLWQWAWRP SPFDVLPTSP WLQLFINARN WTQEDSC
Length:1,337
Mass (Da):144,629
Last modified:December 21, 2004 - v1
Checksum:iDC0DD64F17F80030
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti306 – 3061F → L in AAI14998 (PubMed:15489334).Curated
Sequence conflicti594 – 5974GDKR → WGPQ in BAD32216 (PubMed:15368895).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL645902 Genomic DNA. Translation: CAI24440.1.
BC114997 mRNA. Translation: AAI14998.1.
AK172938 mRNA. Translation: BAD32216.1.
CCDSiCCDS48823.1.
RefSeqiNP_001152991.1. NM_001159519.1.
UniGeneiMm.340288.

Genome annotation databases

EnsembliENSMUST00000021282; ENSMUSP00000021282; ENSMUSG00000020899.
GeneIDi237823.
KEGGimmu:237823.
UCSCiuc007jot.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL645902 Genomic DNA. Translation: CAI24440.1.
BC114997 mRNA. Translation: AAI14998.1.
AK172938 mRNA. Translation: BAD32216.1.
CCDSiCCDS48823.1.
RefSeqiNP_001152991.1. NM_001159519.1.
UniGeneiMm.340288.

3D structure databases

ProteinModelPortaliQ5SUR0.
SMRiQ5SUR0. Positions 304-569, 1062-1301.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000021282.

PTM databases

iPTMnetiQ5SUR0.
PhosphoSiteiQ5SUR0.

Proteomic databases

EPDiQ5SUR0.
MaxQBiQ5SUR0.
PaxDbiQ5SUR0.
PeptideAtlasiQ5SUR0.
PRIDEiQ5SUR0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000021282; ENSMUSP00000021282; ENSMUSG00000020899.
GeneIDi237823.
KEGGimmu:237823.
UCSCiuc007jot.2. mouse.

Organism-specific databases

CTDi5198.
MGIiMGI:2684864. Pfas.
RougeiSearch...

Phylogenomic databases

eggNOGiKOG1907. Eukaryota.
COG0046. LUCA.
COG0047. LUCA.
GeneTreeiENSGT00390000007600.
HOGENOMiHOG000261358.
InParanoidiQ5SUR0.
KOiK01952.
OMAiECLENIC.
OrthoDBiEOG7353X4.
PhylomeDBiQ5SUR0.
TreeFamiTF106371.

Enzyme and pathway databases

UniPathwayiUPA00074; UER00128.
ReactomeiR-MMU-73817. Purine ribonucleoside monophosphate biosynthesis.

Miscellaneous databases

PROiQ5SUR0.
SOURCEiSearch...

Gene expression databases

BgeeiQ5SUR0.
ExpressionAtlasiQ5SUR0. baseline and differential.
GenevisibleiQ5SUR0. MM.

Family and domain databases

Gene3Di3.30.1330.10. 2 hits.
3.40.50.880. 1 hit.
HAMAPiMF_00419. PurL_1.
InterProiIPR010918. AIR_synth_C_dom.
IPR029062. Class_I_gatase-like.
IPR017926. GATASE.
IPR010073. PRibForGlyAmidine_synth.
IPR016188. PurM-like_N.
[Graphical view]
PfamiPF02769. AIRS_C. 2 hits.
[Graphical view]
SUPFAMiSSF52317. SSF52317. 1 hit.
SSF56042. SSF56042. 2 hits.
TIGRFAMsiTIGR01735. FGAM_synt. 1 hit.
PROSITEiPS51273. GATASE_TYPE_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
  3. "Prediction of the coding sequences of mouse homologues of KIAA gene: IV. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
    Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S., Saga Y., Seino S., Nishimura M., Kaisho T., Hoshino K., Kitamura H., Nagase T., Ohara O., Koga H.
    DNA Res. 11:205-218(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 594-1337.
    Tissue: Pancreatic islet.
  4. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Brown adipose tissue, Heart, Kidney, Liver, Lung, Pancreas, Spleen and Testis.

Entry informationi

Entry nameiPUR4_MOUSE
AccessioniPrimary (citable) accession number: Q5SUR0
Secondary accession number(s): A4FUJ6, Q6A080
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: December 21, 2004
Last modified: July 6, 2016
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.