Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Phosphoribosylformylglycinamidine synthase

Gene

Pfas

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Phosphoribosylformylglycinamidine synthase involved in the purines biosynthetic pathway. Catalyzes the ATP-dependent conversion of formylglycinamide ribonucleotide (FGAR) and glutamine to yield formylglycinamidine ribonucleotide (FGAM) and glutamate (By similarity).By similarity

Catalytic activityi

ATP + N2-formyl-N1-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O = ADP + phosphate + 2-(formamido)-N1-(5-phospho-D-ribosyl)acetamidine + L-glutamate.

Pathwayi: IMP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Phosphoribosylformylglycinamidine synthase (Pfas)
  2. Trifunctional purine biosynthetic protein adenosine-3 (Gart), Trifunctional purine biosynthetic protein adenosine-3 (Gart)
This subpathway is part of the pathway IMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide, the pathway IMP biosynthesis via de novo pathway and in Purine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei705ATP; via carbonyl oxygenBy similarity1
Metal bindingi706MagnesiumBy similarity1
Metal bindingi745MagnesiumBy similarity1
Metal bindingi749MagnesiumBy similarity1
Metal bindingi908MagnesiumBy similarity1
Binding sitei910ATPBy similarity1
Active sitei1157NucleophileBy similarity1
Active sitei1296By similarity1
Active sitei1298By similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi322 – 333ATPSequence analysisAdd BLAST12
Nucleotide bindingi402 – 404ATPBy similarity3

GO - Molecular functioni

GO - Biological processi

  • 'de novo' IMP biosynthetic process Source: UniProtKB-UniPathway
  • anterior head development Source: MGI
  • glutamine metabolic process Source: UniProtKB-KW
  • response to drug Source: Ensembl

Keywordsi

Molecular functionLigase
Biological processPurine biosynthesis
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-73817 Purine ribonucleoside monophosphate biosynthesis
UniPathwayiUPA00074; UER00128

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoribosylformylglycinamidine synthase (EC:6.3.5.3)
Short name:
FGAM synthase
Short name:
FGAMS
Alternative name(s):
Formylglycinamide ribonucleotide amidotransferase
Short name:
FGAR amidotransferase
Short name:
FGAR-AT
Formylglycinamide ribotide amidotransferase
Gene namesi
Name:Pfas
Synonyms:Kiaa0361
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:2684864 Pfas

Subcellular locationi

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003708481 – 1337Phosphoribosylformylglycinamidine synthaseAdd BLAST1337

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei215PhosphoserineBy similarity1
Modified residuei619PhosphothreonineBy similarity1
Modified residuei622PhosphothreonineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ5SUR0
MaxQBiQ5SUR0
PaxDbiQ5SUR0
PeptideAtlasiQ5SUR0
PRIDEiQ5SUR0

PTM databases

iPTMnetiQ5SUR0
PhosphoSitePlusiQ5SUR0

Expressioni

Gene expression databases

BgeeiENSMUSG00000020899
ExpressionAtlasiQ5SUR0 baseline and differential
GenevisibleiQ5SUR0 MM

Interactioni

Protein-protein interaction databases

BioGridi231910, 1 interactor
STRINGi10090.ENSMUSP00000021282

Structurei

3D structure databases

ProteinModelPortaliQ5SUR0
SMRiQ5SUR0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1063 – 1301Glutamine amidotransferase type-1Add BLAST239

Sequence similaritiesi

In the N-terminal section; belongs to the FGAMS family.Curated

Keywords - Domaini

Glutamine amidotransferase

Phylogenomic databases

eggNOGiKOG1907 Eukaryota
COG0046 LUCA
COG0047 LUCA
GeneTreeiENSGT00390000007600
HOGENOMiHOG000261358
InParanoidiQ5SUR0
KOiK01952
OMAiSLSANWM
OrthoDBiEOG091G00SI
PhylomeDBiQ5SUR0
TreeFamiTF106371

Family and domain databases

Gene3Di3.30.1330.10, 2 hits
3.40.50.880, 1 hit
3.90.650.10, 2 hits
HAMAPiMF_00419 PurL_1, 1 hit
InterProiView protein in InterPro
IPR029062 Class_I_gatase-like
IPR017926 GATASE
IPR010073 PRibForGlyAmidine_synth
IPR010918 PurM-like_C_dom
IPR036676 PurM-like_C_sf
IPR036921 PurM-like_N_sf
IPR036604 PurS-like_sf
PfamiView protein in Pfam
PF02769 AIRS_C, 2 hits
SUPFAMiSSF52317 SSF52317, 1 hit
SSF55326 SSF55326, 2 hits
SSF56042 SSF56042, 2 hits
SSF82697 SSF82697, 1 hit
TIGRFAMsiTIGR01735 FGAM_synt, 1 hit
PROSITEiView protein in PROSITE
PS51273 GATASE_TYPE_1, 1 hit

Sequencei

Sequence statusi: Complete.

Q5SUR0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAPVLHFYVR PSGHEGAASG RVFRRLQEKL PTLQSVETEL CYNVHWAAET
60 70 80 90 100
LPWAEEMKKL MWLFGCPLVR DDVAQEPWLV PGSNDLLLEV GPRLNFSTPA
110 120 130 140 150
STNIVSVCQA AGLRAVDRVE TTRRYRLSFT DHPTAEMEAI SLAALHDRMT
160 170 180 190 200
EQHYPDPIQS FSPQSIPAPL KGSIDILAEG RPALEKANQE LGLALDSWDL
210 220 230 240 250
DFYTKRFQEL QRNPSTVEVF DLAQSNSEHS RHWFFKGQLH VDGKKLAHSL
260 270 280 290 300
FESIMSTQAS SNPNNVLKFC DNSSAIQGKK VKFLRPEDST RPSCFQQQQG
310 320 330 340 350
LRHVVFTAET HNFPTGVAPF SGATTGTGGR IRDVQCTGRG AHVVAGTAGY
360 370 380 390 400
CFGNLHIPDY NLPWEDPSFQ YPGNFARPLE VAIEASNGAS DYGNKFGEPV
410 420 430 440 450
LAGFARSLGL QLPDGQRREW IKPIMFSGGI GSMEAKHVGK KPPEPGMEVV
460 470 480 490 500
KVGGPVYRIG VGGGAASSVQ VQGDNTSDLD FGAVQRGDPE MEQKMNRVIR
510 520 530 540 550
ACVEAPGGNP ICSLHDQGAG GNGNVLKELS DPEGAIIYTS RFQLGDPTLN
560 570 580 590 600
ALEIWGAEYQ ESNALLLRPS DRDFLSRASA RERCPACFVG TITGDKRIVL
610 620 630 640 650
VDDRECLVGK TGQGDAPLTP PTPVDLDLDW VLGKMPQKEF FLQRKPPVLQ
660 670 680 690 700
PLALPPELSV RQALNRVLRL PAVASKRYLT NKVDRSVGGL VAQQQCVGPL
710 720 730 740 750
QTPLADVAVV ALSHQECIGA ATALGEQPVK SLLDPKAAAR LAVSEALTNL
760 770 780 790 800
VFALVTDLRD VKCSGNWMWA AKLPGEGAAL ADACEAMVAV MAALGVAVDG
810 820 830 840 850
GKDSLSMAAR VGTETVQAPG SLVISAYAVC PDITATVTPD LKHPGGKGHL
860 870 880 890 900
LYVPLSPGQH RLGGTALAQC FSQLGEHPPD LDLPENLVRA FHITQGLLKE
910 920 930 940 950
CRLCSGHDVS DGGLVTCLLE MAFAGNCGIE VDVPAPGIHA LPVLFAEEPG
960 970 980 990 1000
LVLEVQEADV AGVRQRYESA GLRCLELGHT GEAGPQAMAR ISVNKAVVVE
1010 1020 1030 1040 1050
EPVGELRALW EETSFQLDLL QAEPRCVIEE KQGLKERTGP SYYLPPTFPV
1060 1070 1080 1090 1100
ASVPCKPGGP VPRVAILREE GSNGDREMAD AFHLAGFEVW DVTMQDLCSG
1110 1120 1130 1140 1150
AIRLDTFRGV AFVGGFSYAD VLGSAKGWAA AVTFNPQARE ELGRFRRRPD
1160 1170 1180 1190 1200
TFSLGVCNGC QLLALLGWVG SDPSEEQAEP GQDSQPTQPG LLLRHNLSGR
1210 1220 1230 1240 1250
FESRWATVRV EPGPALMLRG MEGSVLPVWS AHGEGYMAFS SPELQAKIEA
1260 1270 1280 1290 1300
KGLVPLHWAD DDGNPTEQYP LNPNGSPGGI AGICSQDGRH LALMPHPERA
1310 1320 1330
VRLWQWAWRP SPFDVLPTSP WLQLFINARN WTQEDSC
Length:1,337
Mass (Da):144,629
Last modified:December 21, 2004 - v1
Checksum:iDC0DD64F17F80030
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti306F → L in AAI14998 (PubMed:15489334).Curated1
Sequence conflicti594 – 597GDKR → WGPQ in BAD32216 (PubMed:15368895).Curated4

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL645902 Genomic DNA No translation available.
BC114997 mRNA Translation: AAI14998.1
AK172938 mRNA Translation: BAD32216.1
CCDSiCCDS48823.1
RefSeqiNP_001152991.1, NM_001159519.1
UniGeneiMm.340288

Genome annotation databases

EnsembliENSMUST00000021282; ENSMUSP00000021282; ENSMUSG00000020899
GeneIDi237823
KEGGimmu:237823
UCSCiuc007jot.2 mouse

Similar proteinsi

Entry informationi

Entry nameiPUR4_MOUSE
AccessioniPrimary (citable) accession number: Q5SUR0
Secondary accession number(s): A4FUJ6, Q6A080
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: December 21, 2004
Last modified: March 28, 2018
This is version 112 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health