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Protein

Adenosine deaminase domain-containing protein 1

Gene

Adad1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Plays a role in spermatogenesis. Binds to RNA but not to DNA.1 Publication

GO - Molecular functioni

  • adenosine deaminase activity Source: InterPro
  • RNA binding Source: MGI

GO - Biological processi

  • multicellular organism development Source: UniProtKB-KW
  • RNA processing Source: InterPro
  • spermatid development Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Differentiation, Spermatogenesis

Keywords - Ligandi

RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Adenosine deaminase domain-containing protein 1
Alternative name(s):
Testis nuclear RNA-binding protein
Gene namesi
Name:Adad1
Synonyms:Tenr
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:103258. Adad1.

Subcellular locationi

  • Nucleus 1 Publication

GO - Cellular componenti

  • nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Mice exhibit spermatozoa retention in stage IX tubules and a reduction in the number of sperm in the epididymis, leading to a reduction in fertility.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 619619Adenosine deaminase domain-containing protein 1PRO_0000309521Add
BLAST

Proteomic databases

PaxDbiQ5SUE7.
PRIDEiQ5SUE7.

PTM databases

PhosphoSiteiQ5SUE7.

Expressioni

Tissue specificityi

Testis-specific. Detected in round spermatid cells from stage II-XI (at protein level). Expressed in germ cells from mid-pachytene spermatocytes to mid-round spermatids.1 Publication

Gene expression databases

BgeeiQ5SUE7.
ExpressionAtlasiQ5SUE7. baseline and differential.
GenevisibleiQ5SUE7. MM.

Interactioni

Protein-protein interaction databases

IntActiQ5SUE7. 1 interaction.
MINTiMINT-8174090.
STRINGi10090.ENSMUSP00000115260.

Structurei

3D structure databases

ProteinModelPortaliQ5SUE7.
SMRiQ5SUE7. Positions 245-619.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini138 – 20669DRBMPROSITE-ProRule annotationAdd
BLAST
Domaini291 – 617327A to I editasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the ADAD family.Curated
Contains 1 A to I editase domain.PROSITE-ProRule annotation
Contains 1 DRBM (double-stranded RNA-binding) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG2777. Eukaryota.
ENOG410XT0Z. LUCA.
GeneTreeiENSGT00550000074412.
HOVERGENiHBG058021.
InParanoidiQ5SUE7.
OMAiLHDSHAV.
OrthoDBiEOG7K0ZC7.
PhylomeDBiQ5SUE7.
TreeFamiTF315806.

Family and domain databases

Gene3Di3.30.160.20. 1 hit.
InterProiIPR002466. A_deamin.
IPR014720. dsRBD_dom.
[Graphical view]
PfamiPF02137. A_deamin. 1 hit.
PF00035. dsrm. 1 hit.
[Graphical view]
SMARTiSM00552. ADEAMc. 1 hit.
SM00358. DSRM. 1 hit.
[Graphical view]
PROSITEiPS50141. A_DEAMIN_EDITASE. 1 hit.
PS50137. DS_RBD. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q5SUE7-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MATAGGSRRA PVPGPRLGLP LAAHLPASLG GEGAKDSVGG EKTSGNNDWF
60 70 80 90 100
QSSRVPSFAQ MLKKNLPVQP SAQTVTLPTG YSSESCSLSN MASKVTQVTG
110 120 130 140 150
NFPEPLLSKG LSSISNPVLP PKKLPKEFIM KYKRGEINPV SALHQFAQMQ
160 170 180 190 200
RVQLDLKETV TTGNVMGPYF AFCAVVDGIQ YKTGLGQNKK ESRSNAAKLA
210 220 230 240 250
LDELLQLDEP EPRVLEPAGP PPIPAEPVVT PEAAYVSKVQ YEGRQVQYAK
260 270 280 290 300
ISQLVKETFG QLISNHSQYL KCSSSLAAFI IERAGHHEVV AIGTGEYNYS
310 320 330 340 350
QCIKPNGRVL HDTHAVVTAR RSLLRYFYRQ LLLFYSKNPA MMEKSIFCTE
360 370 380 390 400
PASNLLTLKQ NINLYLYMNQ LPKGSAQIKS QLRLNPHSIS AFEANEELSL
410 420 430 440 450
HVAVEGKIYL TVYCSADGVN RVNSMSSSDK LTRWEVLGVQ GALLSHFIQP
460 470 480 490 500
VYISSILVGD GNCNDTRGLE IAINQRVDDA LTSKLPMFYL VNRPHISLVP
510 520 530 540 550
TAYPLQINLD HKSLSLNWAQ GDNSLEIVDG LNGKITESSP FKSGLSMASR
560 570 580 590 600
LCKAAMLSRF NLLAKEAKTD DLLEARTYHA AKCMSGPYQE AKALLKAYLQ
610
QHGYGSWIVK SPCIEQFSM
Length:619
Mass (Da):67,840
Last modified:December 21, 2004 - v1
Checksum:i168EC0352B1B70A7
GO
Isoform 2 (identifier: Q5SUE7-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     220-222: PPP → KGN
     223-619: Missing.

Note: No experimental confirmation available.
Show »
Length:222
Mass (Da):23,632
Checksum:iA94D2F3D307BAA67
GO
Isoform 3 (identifier: Q5SUE7-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     619-619: M → DKLRRKYFDKFMGWLFQI

Show »
Length:636
Mass (Da):70,083
Checksum:iC055FA62EE18F187
GO

Sequence cautioni

The sequence CAM18414.1 differs from that shown. Reason: Erroneous gene model prediction. Curated
The sequence CAM28072.1 differs from that shown. Reason: Erroneous gene model prediction. Curated
The sequence CAM28078.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti255 – 2551V → L in CAA59168 (PubMed:7543294).Curated
Sequence conflicti362 – 3621I → Y in CAA59168 (PubMed:7543294).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei220 – 2223PPP → KGN in isoform 2. 1 PublicationVSP_029227
Alternative sequencei223 – 619397Missing in isoform 2. 1 PublicationVSP_029228Add
BLAST
Alternative sequencei619 – 6191M → DKLRRKYFDKFMGWLFQI in isoform 3. 1 PublicationVSP_029229

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X84693 mRNA. Translation: CAA59168.1.
AL645753 Genomic DNA. Translation: CAM28065.1.
AL645753 Genomic DNA. Translation: CAM28066.1.
AL645807 Genomic DNA. Translation: CAM28072.1. Sequence problems.
AL645966 Genomic DNA. Translation: CAI26206.1.
AL645966 Genomic DNA. Translation: CAM28078.1. Sequence problems.
AL662823 Genomic DNA. Translation: CAM18412.1.
AL662823 Genomic DNA. Translation: CAM18414.1. Sequence problems.
BC107346 mRNA. Translation: AAI07347.1.
AK133008 mRNA. Translation: BAE21467.1.
AV255684 mRNA. No translation available.
AF195956 Genomic DNA. Translation: AAF32273.1.
CCDSiCCDS17315.1. [Q5SUE7-1]
PIRiI48840.
RefSeqiNP_033376.2. NM_009350.3. [Q5SUE7-1]
XP_006535498.1. XM_006535435.1. [Q5SUE7-3]
UniGeneiMm.8122.

Genome annotation databases

EnsembliENSMUST00000144629; ENSMUSP00000115260; ENSMUSG00000027719. [Q5SUE7-1]
GeneIDi21744.
KEGGimmu:21744.
UCSCiuc008pag.1. mouse. [Q5SUE7-2]
uc008pah.1. mouse. [Q5SUE7-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X84693 mRNA. Translation: CAA59168.1.
AL645753 Genomic DNA. Translation: CAM28065.1.
AL645753 Genomic DNA. Translation: CAM28066.1.
AL645807 Genomic DNA. Translation: CAM28072.1. Sequence problems.
AL645966 Genomic DNA. Translation: CAI26206.1.
AL645966 Genomic DNA. Translation: CAM28078.1. Sequence problems.
AL662823 Genomic DNA. Translation: CAM18412.1.
AL662823 Genomic DNA. Translation: CAM18414.1. Sequence problems.
BC107346 mRNA. Translation: AAI07347.1.
AK133008 mRNA. Translation: BAE21467.1.
AV255684 mRNA. No translation available.
AF195956 Genomic DNA. Translation: AAF32273.1.
CCDSiCCDS17315.1. [Q5SUE7-1]
PIRiI48840.
RefSeqiNP_033376.2. NM_009350.3. [Q5SUE7-1]
XP_006535498.1. XM_006535435.1. [Q5SUE7-3]
UniGeneiMm.8122.

3D structure databases

ProteinModelPortaliQ5SUE7.
SMRiQ5SUE7. Positions 245-619.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ5SUE7. 1 interaction.
MINTiMINT-8174090.
STRINGi10090.ENSMUSP00000115260.

PTM databases

PhosphoSiteiQ5SUE7.

Proteomic databases

PaxDbiQ5SUE7.
PRIDEiQ5SUE7.

Protocols and materials databases

DNASUi21744.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000144629; ENSMUSP00000115260; ENSMUSG00000027719. [Q5SUE7-1]
GeneIDi21744.
KEGGimmu:21744.
UCSCiuc008pag.1. mouse. [Q5SUE7-2]
uc008pah.1. mouse. [Q5SUE7-1]

Organism-specific databases

CTDi132612.
MGIiMGI:103258. Adad1.

Phylogenomic databases

eggNOGiKOG2777. Eukaryota.
ENOG410XT0Z. LUCA.
GeneTreeiENSGT00550000074412.
HOVERGENiHBG058021.
InParanoidiQ5SUE7.
OMAiLHDSHAV.
OrthoDBiEOG7K0ZC7.
PhylomeDBiQ5SUE7.
TreeFamiTF315806.

Miscellaneous databases

NextBioi301028.
PROiQ5SUE7.
SOURCEiSearch...

Gene expression databases

BgeeiQ5SUE7.
ExpressionAtlasiQ5SUE7. baseline and differential.
GenevisibleiQ5SUE7. MM.

Family and domain databases

Gene3Di3.30.160.20. 1 hit.
InterProiIPR002466. A_deamin.
IPR014720. dsRBD_dom.
[Graphical view]
PfamiPF02137. A_deamin. 1 hit.
PF00035. dsrm. 1 hit.
[Graphical view]
SMARTiSM00552. ADEAMc. 1 hit.
SM00358. DSRM. 1 hit.
[Graphical view]
PROSITEiPS50141. A_DEAMIN_EDITASE. 1 hit.
PS50137. DS_RBD. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Distribution of Tenr, an RNA-binding protein, in a lattice-like network within the spermatid nucleus in the mouse."
    Schumacher J.M., Lee K., Edelhoff S., Braun R.E.
    Biol. Reprod. 52:1274-1283(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), RNA-BINDING, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    Strain: CD-1.
    Tissue: Testis.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
  4. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 174-619 (ISOFORM 1), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 438-619 (ISOFORM 3).
    Strain: C57BL/6J.
    Tissue: Testis.
  5. "Congenic mapping of the type 1 diabetes locus, Idd3, to a 780-kb region of mouse chromosome 3: identification of a candidate segment of ancestral DNA by haplotype mapping."
    Lyons P.A., Armitage N., Argentina F., Denny P., Hill N.J., Lord C.J., Wilusz M.B., Peterson L.B., Wicker L.S., Todd J.A.
    Genome Res. 10:446-453(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 539-619 (ISOFORM 1).
    Strain: C57BL/6J.
  6. "Disruption of murine Tenr results in teratospermia and male infertility."
    Connolly C.M., Dearth A.T., Braun R.E.
    Dev. Biol. 278:13-21(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.
  7. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Testis.

Entry informationi

Entry nameiADAD1_MOUSE
AccessioniPrimary (citable) accession number: Q5SUE7
Secondary accession number(s): A2AAD7
, A2AAD8, A2AAV1, Q08EL2, Q3V0N7, Q62309, Q9JLB6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 13, 2007
Last sequence update: December 21, 2004
Last modified: May 11, 2016
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.