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Protein

Heterochromatin protein 1-binding protein 3

Gene

HP1BP3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of heterochromatin that maintains heterochromatin integrity during G1/S progression and regulates the duration of G1 phase to critically influence cell proliferative capacity (PubMed:24830416). Mediates chromatin condensation during hypoxia, leading to increased tumor cell viability, radio-resistance, chemo-resistance and self-renewal(PubMed:25100860).2 Publications

GO - Molecular functioni

  • DNA binding Source: UniProtKB
  • nucleosome binding Source: UniProtKB

GO - Biological processi

  • cellular response to hypoxia Source: UniProtKB
  • heterochromatin organization Source: UniProtKB
  • nucleosome assembly Source: InterPro
  • regulation of cell proliferation Source: UniProtKB
  • regulation of nucleus size Source: UniProtKB
  • regulation of transcription, DNA-templated Source: UniProtKB
Complete GO annotation...

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Heterochromatin protein 1-binding protein 3
Alternative name(s):
Protein HP1-BP741 Publication
Gene namesi
Name:HP1BP3
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

HGNCiHGNC:24973. HP1BP3.

Subcellular locationi

GO - Cellular componenti

  • chromosome Source: UniProtKB
  • nucleosome Source: InterPro
  • nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Chromosome, Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi50809.
OpenTargetsiENSG00000127483.
PharmGKBiPA142671673.

Polymorphism and mutation databases

BioMutaiHP1BP3.
DMDMi74743691.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00003396422 – 553Heterochromatin protein 1-binding protein 3Add BLAST552

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineCombined sources1
Modified residuei6PhosphoserineCombined sources1
Modified residuei51PhosphothreonineCombined sources1
Modified residuei85PhosphothreonineBy similarity1
Modified residuei142PhosphoserineCombined sources1
Modified residuei155PhosphoserineCombined sources1
Modified residuei156PhosphoserineCombined sources1
Modified residuei190N6-acetyllysineBy similarity1
Modified residuei248PhosphoserineCombined sources1
Modified residuei249PhosphoserineCombined sources1
Modified residuei441PhosphoserineCombined sources1
Modified residuei442PhosphoserineCombined sources1
Modified residuei446PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ5SSJ5.
MaxQBiQ5SSJ5.
PaxDbiQ5SSJ5.
PeptideAtlasiQ5SSJ5.
PRIDEiQ5SSJ5.

PTM databases

iPTMnetiQ5SSJ5.
PhosphoSitePlusiQ5SSJ5.
SwissPalmiQ5SSJ5.

Expressioni

Gene expression databases

BgeeiENSG00000127483.
CleanExiHS_HP1BP3.
ExpressionAtlasiQ5SSJ5. baseline and differential.
GenevisibleiQ5SSJ5. HS.

Organism-specific databases

HPAiHPA028215.
HPA054295.

Interactioni

Subunit structurei

Interacts (via PxVxL motif) with CBX5 (via Trp-174).1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
MEOX2A4D1273EBI-2880687,EBI-10172134

Protein-protein interaction databases

BioGridi119128. 80 interactors.
IntActiQ5SSJ5. 23 interactors.
MINTiMINT-4719917.
STRINGi9606.ENSP00000312625.

Structurei

Secondary structure

1553
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi161 – 175Combined sources15
Helixi180 – 190Combined sources11
Helixi194 – 197Combined sources4
Turni198 – 202Combined sources5
Helixi203 – 214Combined sources12
Turni224 – 226Combined sources3
Turni231 – 234Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2RQPNMR-A153-237[»]
ProteinModelPortaliQ5SSJ5.
SMRiQ5SSJ5.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ5SSJ5.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini157 – 232H15 1PROSITE-ProRule annotationAdd BLAST76
Domaini255 – 330H15 2PROSITE-ProRule annotationAdd BLAST76
Domaini337 – 413H15 3PROSITE-ProRule annotationAdd BLAST77

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi255 – 259PxVxL motif1 Publication5

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi454 – 553Lys-richAdd BLAST100

Domaini

A central region that included the first H15 (linker histone H1/H5 globular) domain binds at the entry/exit site of the nucleosomal DNA.1 Publication

Sequence similaritiesi

Contains 3 H15 (linker histone H1/H5 globular) domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG4012. Eukaryota.
ENOG4112541. LUCA.
GeneTreeiENSGT00510000047652.
HOVERGENiHBG097678.
InParanoidiQ5SSJ5.
OMAiKTIPAWA.
OrthoDBiEOG091G0LIU.
PhylomeDBiQ5SSJ5.
TreeFamiTF106395.

Family and domain databases

Gene3Di1.10.10.10. 3 hits.
InterProiIPR005818. Histone_H1/H5_H15.
IPR033061. HP1BP3.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PANTHERiPTHR15832:SF1. PTHR15832:SF1. 1 hit.
PfamiPF00538. Linker_histone. 3 hits.
[Graphical view]
SMARTiSM00526. H15. 3 hits.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 3 hits.
PROSITEiPS51504. H15. 3 hits.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q5SSJ5-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MATDTSQGEL VHPKALPLIV GAQLIHADKL GEKVEDSTMP IRRTVNSTRE
60 70 80 90 100
TPPKSKLAEG EEEKPEPDIS SEESVSTVEE QENETPPATS SEAEQPKGEP
110 120 130 140 150
ENEEKEENKS SEETKKDEKD QSKEKEKKVK KTIPSWATLS ASQLARAQKQ
160 170 180 190 200
TPMASSPRPK MDAILTEAIK ACFQKSGASV VAIRKYIIHK YPSLELERRG
210 220 230 240 250
YLLKQALKRE LNRGVIKQVK GKGASGSFVV VQKSRKTPQK SRNRKNRSSA
260 270 280 290 300
VDPEPQVKLE DVLPLAFTRL CEPKEASYSL IRKYVSQYYP KLRVDIRPQL
310 320 330 340 350
LKNALQRAVE RGQLEQITGK GASGTFQLKK SGEKPLLGGS LMEYAILSAI
360 370 380 390 400
AAMNEPKTCS TTALKKYVLE NHPGTNSNYQ MHLLKKTLQK CEKNGWMEQI
410 420 430 440 450
SGKGFSGTFQ LCFPYYPSPG VLFPKKEPDD SRDEDEDEDE SSEEDSEDEE
460 470 480 490 500
PPPKRRLQKK TPAKSPGKAA SVKQRGSKPA PKVSAAQRGK ARPLPKKAPP
510 520 530 540 550
KAKTPAKKTR PSSTVIKKPS GGSSKKPATS ARKEVKLPGK GKSTMKKSFR

VKK
Length:553
Mass (Da):61,207
Last modified:December 21, 2004 - v1
Checksum:iD18B28EE0156F09B
GO
Isoform 2 (identifier: Q5SSJ5-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-38: Missing.

Show »
Length:515
Mass (Da):57,211
Checksum:i130AD6B3FFD97525
GO
Isoform 3 (identifier: Q5SSJ5-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-152: Missing.

Show »
Length:401
Mass (Da):44,435
Checksum:i31A31A118D2508B7
GO
Isoform 4 (identifier: Q5SSJ5-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     117-133: DEKDQSKEKEKKVKKTI → ERADSIHSTLFIIGQNS
     134-553: Missing.

Show »
Length:133
Mass (Da):14,594
Checksum:i87E1DE4A3A8FB907
GO

Sequence cautioni

The sequence AAF14871 differs from that shown. Reason: Frameshift at positions 226, 237 and 243.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti262V → G in AAF14871 (PubMed:10931946).Curated1
Sequence conflicti267F → S in BAF83941 (PubMed:14702039).Curated1
Sequence conflicti499P → S in BAG64972 (PubMed:14702039).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0341681 – 152Missing in isoform 3. 2 PublicationsAdd BLAST152
Alternative sequenceiVSP_0341691 – 38Missing in isoform 2. 1 PublicationAdd BLAST38
Alternative sequenceiVSP_034170117 – 133DEKDQ…VKKTI → ERADSIHSTLFIIGQNS in isoform 4. 1 PublicationAdd BLAST17
Alternative sequenceiVSP_034171134 – 553Missing in isoform 4. 1 PublicationAdd BLAST420

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF113534 mRNA. Translation: AAF14871.1. Frameshift.
AK023129 mRNA. Translation: BAG51160.1.
AK291252 mRNA. Translation: BAF83941.1.
AK304065 mRNA. Translation: BAG64972.1.
AL833978 mRNA. Translation: CAD38822.1.
AL606477, AL663074 Genomic DNA. Translation: CAI12528.1.
AL606477, AL663074 Genomic DNA. Translation: CAI12530.1.
AL606477 Genomic DNA. Translation: CAI12531.1.
AL606477 Genomic DNA. Translation: CAI12532.1.
AL606477, AL663074 Genomic DNA. Translation: CAI12533.1.
AL663074, AL606477 Genomic DNA. Translation: CAI23387.1.
AL663074, AL606477 Genomic DNA. Translation: CAI23388.1.
AL663074, AL606477 Genomic DNA. Translation: CAI23389.1.
CH471134 Genomic DNA. Translation: EAW94953.1.
BC032139 mRNA. Translation: AAH32139.1.
BC045660 mRNA. Translation: AAH45660.1.
CCDSiCCDS30621.1. [Q5SSJ5-1]
RefSeqiNP_057371.2. NM_016287.3. [Q5SSJ5-1]
XP_005245932.1. XM_005245875.4. [Q5SSJ5-1]
XP_005245933.1. XM_005245876.3. [Q5SSJ5-1]
XP_005245934.1. XM_005245877.4. [Q5SSJ5-1]
XP_005245935.1. XM_005245878.4. [Q5SSJ5-1]
XP_005245936.1. XM_005245879.4. [Q5SSJ5-2]
XP_011539834.1. XM_011541532.1. [Q5SSJ5-2]
XP_011539835.1. XM_011541533.1. [Q5SSJ5-2]
XP_011539836.1. XM_011541534.2. [Q5SSJ5-2]
XP_016856882.1. XM_017001393.1. [Q5SSJ5-1]
XP_016856883.1. XM_017001394.1. [Q5SSJ5-2]
UniGeneiHs.142442.

Genome annotation databases

EnsembliENST00000312239; ENSP00000312625; ENSG00000127483. [Q5SSJ5-1]
ENST00000375000; ENSP00000364139; ENSG00000127483. [Q5SSJ5-5]
ENST00000375003; ENSP00000364142; ENSG00000127483. [Q5SSJ5-3]
GeneIDi50809.
KEGGihsa:50809.
UCSCiuc001bdw.2. human. [Q5SSJ5-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF113534 mRNA. Translation: AAF14871.1. Frameshift.
AK023129 mRNA. Translation: BAG51160.1.
AK291252 mRNA. Translation: BAF83941.1.
AK304065 mRNA. Translation: BAG64972.1.
AL833978 mRNA. Translation: CAD38822.1.
AL606477, AL663074 Genomic DNA. Translation: CAI12528.1.
AL606477, AL663074 Genomic DNA. Translation: CAI12530.1.
AL606477 Genomic DNA. Translation: CAI12531.1.
AL606477 Genomic DNA. Translation: CAI12532.1.
AL606477, AL663074 Genomic DNA. Translation: CAI12533.1.
AL663074, AL606477 Genomic DNA. Translation: CAI23387.1.
AL663074, AL606477 Genomic DNA. Translation: CAI23388.1.
AL663074, AL606477 Genomic DNA. Translation: CAI23389.1.
CH471134 Genomic DNA. Translation: EAW94953.1.
BC032139 mRNA. Translation: AAH32139.1.
BC045660 mRNA. Translation: AAH45660.1.
CCDSiCCDS30621.1. [Q5SSJ5-1]
RefSeqiNP_057371.2. NM_016287.3. [Q5SSJ5-1]
XP_005245932.1. XM_005245875.4. [Q5SSJ5-1]
XP_005245933.1. XM_005245876.3. [Q5SSJ5-1]
XP_005245934.1. XM_005245877.4. [Q5SSJ5-1]
XP_005245935.1. XM_005245878.4. [Q5SSJ5-1]
XP_005245936.1. XM_005245879.4. [Q5SSJ5-2]
XP_011539834.1. XM_011541532.1. [Q5SSJ5-2]
XP_011539835.1. XM_011541533.1. [Q5SSJ5-2]
XP_011539836.1. XM_011541534.2. [Q5SSJ5-2]
XP_016856882.1. XM_017001393.1. [Q5SSJ5-1]
XP_016856883.1. XM_017001394.1. [Q5SSJ5-2]
UniGeneiHs.142442.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2RQPNMR-A153-237[»]
ProteinModelPortaliQ5SSJ5.
SMRiQ5SSJ5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi119128. 80 interactors.
IntActiQ5SSJ5. 23 interactors.
MINTiMINT-4719917.
STRINGi9606.ENSP00000312625.

PTM databases

iPTMnetiQ5SSJ5.
PhosphoSitePlusiQ5SSJ5.
SwissPalmiQ5SSJ5.

Polymorphism and mutation databases

BioMutaiHP1BP3.
DMDMi74743691.

Proteomic databases

EPDiQ5SSJ5.
MaxQBiQ5SSJ5.
PaxDbiQ5SSJ5.
PeptideAtlasiQ5SSJ5.
PRIDEiQ5SSJ5.

Protocols and materials databases

DNASUi50809.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000312239; ENSP00000312625; ENSG00000127483. [Q5SSJ5-1]
ENST00000375000; ENSP00000364139; ENSG00000127483. [Q5SSJ5-5]
ENST00000375003; ENSP00000364142; ENSG00000127483. [Q5SSJ5-3]
GeneIDi50809.
KEGGihsa:50809.
UCSCiuc001bdw.2. human. [Q5SSJ5-1]

Organism-specific databases

CTDi50809.
DisGeNETi50809.
GeneCardsiHP1BP3.
HGNCiHGNC:24973. HP1BP3.
HPAiHPA028215.
HPA054295.
MIMi616072. gene.
neXtProtiNX_Q5SSJ5.
OpenTargetsiENSG00000127483.
PharmGKBiPA142671673.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG4012. Eukaryota.
ENOG4112541. LUCA.
GeneTreeiENSGT00510000047652.
HOVERGENiHBG097678.
InParanoidiQ5SSJ5.
OMAiKTIPAWA.
OrthoDBiEOG091G0LIU.
PhylomeDBiQ5SSJ5.
TreeFamiTF106395.

Miscellaneous databases

ChiTaRSiHP1BP3. human.
EvolutionaryTraceiQ5SSJ5.
GeneWikiiHP1BP3.
GenomeRNAii50809.
PROiQ5SSJ5.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000127483.
CleanExiHS_HP1BP3.
ExpressionAtlasiQ5SSJ5. baseline and differential.
GenevisibleiQ5SSJ5. HS.

Family and domain databases

Gene3Di1.10.10.10. 3 hits.
InterProiIPR005818. Histone_H1/H5_H15.
IPR033061. HP1BP3.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PANTHERiPTHR15832:SF1. PTHR15832:SF1. 1 hit.
PfamiPF00538. Linker_histone. 3 hits.
[Graphical view]
SMARTiSM00526. H15. 3 hits.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 3 hits.
PROSITEiPS51504. H15. 3 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiHP1B3_HUMAN
AccessioniPrimary (citable) accession number: Q5SSJ5
Secondary accession number(s): A6NI71
, A8K5D7, B3KMZ8, B4E210, Q05BI0, Q5SSJ6, Q5SWC6, Q6PIM9, Q8NDF0, Q9UHY0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 10, 2008
Last sequence update: December 21, 2004
Last modified: November 30, 2016
This is version 127 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.