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Protein

Heterochromatin protein 1-binding protein 3

Gene

HP1BP3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of heterochromatin that maintains heterochromatin integrity during G1/S progression and regulates the duration of G1 phase to critically influence cell proliferative capacity (PubMed:24830416). Mediates chromatin condensation during hypoxia, leading to increased tumor cell viability, radio-resistance, chemo-resistance and self-renewal(PubMed:25100860).2 Publications

GO - Molecular functioni

  • DNA binding Source: UniProtKB
  • nucleosome binding Source: UniProtKB

GO - Biological processi

  • cellular response to hypoxia Source: UniProtKB
  • heterochromatin organization Source: UniProtKB
  • nucleosome assembly Source: InterPro
  • regulation of cell proliferation Source: UniProtKB
  • regulation of nucleus size Source: UniProtKB
  • regulation of transcription, DNA-templated Source: UniProtKB

Keywordsi

Molecular functionDNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Heterochromatin protein 1-binding protein 3
Alternative name(s):
Protein HP1-BP741 Publication
Gene namesi
Name:HP1BP3
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

EuPathDBiHostDB:ENSG00000127483.17
HGNCiHGNC:24973 HP1BP3
MIMi616072 gene
neXtProtiNX_Q5SSJ5

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chromosome, Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi50809
OpenTargetsiENSG00000127483
PharmGKBiPA142671673

Polymorphism and mutation databases

BioMutaiHP1BP3
DMDMi74743691

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00003396422 – 553Heterochromatin protein 1-binding protein 3Add BLAST552

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineCombined sources1
Modified residuei6PhosphoserineCombined sources1
Modified residuei51PhosphothreonineCombined sources1
Cross-linki64Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei85PhosphothreonineBy similarity1
Cross-linki97Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei142PhosphoserineCombined sources1
Modified residuei155PhosphoserineCombined sources1
Modified residuei156PhosphoserineCombined sources1
Modified residuei190N6-acetyllysineBy similarity1
Modified residuei248PhosphoserineCombined sources1
Modified residuei249PhosphoserineCombined sources1
Cross-linki258Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei441PhosphoserineCombined sources1
Modified residuei442PhosphoserineCombined sources1
Modified residuei446PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ5SSJ5
MaxQBiQ5SSJ5
PaxDbiQ5SSJ5
PeptideAtlasiQ5SSJ5
PRIDEiQ5SSJ5

PTM databases

iPTMnetiQ5SSJ5
PhosphoSitePlusiQ5SSJ5
SwissPalmiQ5SSJ5

Expressioni

Gene expression databases

BgeeiENSG00000127483
CleanExiHS_HP1BP3
ExpressionAtlasiQ5SSJ5 baseline and differential
GenevisibleiQ5SSJ5 HS

Organism-specific databases

HPAiHPA028215
HPA054295

Interactioni

Subunit structurei

Interacts (via PxVxL motif) with CBX5 (via Trp-174).1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
MEOX2P502223EBI-2880687,EBI-748397

Protein-protein interaction databases

BioGridi119128, 113 interactors
IntActiQ5SSJ5, 25 interactors
MINTiQ5SSJ5
STRINGi9606.ENSP00000312625

Structurei

Secondary structure

1553
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi161 – 175Combined sources15
Helixi180 – 190Combined sources11
Helixi194 – 197Combined sources4
Turni198 – 202Combined sources5
Helixi203 – 214Combined sources12
Turni224 – 226Combined sources3
Turni231 – 234Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2RQPNMR-A153-237[»]
ProteinModelPortaliQ5SSJ5
SMRiQ5SSJ5
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ5SSJ5

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini157 – 232H15 1PROSITE-ProRule annotationAdd BLAST76
Domaini255 – 330H15 2PROSITE-ProRule annotationAdd BLAST76
Domaini337 – 413H15 3PROSITE-ProRule annotationAdd BLAST77

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi255 – 259PxVxL motif1 Publication5

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi454 – 553Lys-richAdd BLAST100

Domaini

A central region that included the first H15 (linker histone H1/H5 globular) domain binds at the entry/exit site of the nucleosomal DNA.1 Publication

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG4012 Eukaryota
ENOG4112541 LUCA
GeneTreeiENSGT00900000141023
HOVERGENiHBG097678
InParanoidiQ5SSJ5
OMAiQRCEKNG
OrthoDBiEOG091G0LIU
PhylomeDBiQ5SSJ5
TreeFamiTF106395

Family and domain databases

Gene3Di1.10.10.10, 3 hits
InterProiView protein in InterPro
IPR005818 Histone_H1/H5_H15
IPR033061 HP1BP3
IPR036388 WH-like_DNA-bd_sf
IPR036390 WH_DNA-bd_sf
PANTHERiPTHR15832:SF1 PTHR15832:SF1, 1 hit
PfamiView protein in Pfam
PF00538 Linker_histone, 3 hits
SMARTiView protein in SMART
SM00526 H15, 3 hits
SUPFAMiSSF46785 SSF46785, 3 hits
PROSITEiView protein in PROSITE
PS51504 H15, 3 hits

Sequences (4)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q5SSJ5-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MATDTSQGEL VHPKALPLIV GAQLIHADKL GEKVEDSTMP IRRTVNSTRE
60 70 80 90 100
TPPKSKLAEG EEEKPEPDIS SEESVSTVEE QENETPPATS SEAEQPKGEP
110 120 130 140 150
ENEEKEENKS SEETKKDEKD QSKEKEKKVK KTIPSWATLS ASQLARAQKQ
160 170 180 190 200
TPMASSPRPK MDAILTEAIK ACFQKSGASV VAIRKYIIHK YPSLELERRG
210 220 230 240 250
YLLKQALKRE LNRGVIKQVK GKGASGSFVV VQKSRKTPQK SRNRKNRSSA
260 270 280 290 300
VDPEPQVKLE DVLPLAFTRL CEPKEASYSL IRKYVSQYYP KLRVDIRPQL
310 320 330 340 350
LKNALQRAVE RGQLEQITGK GASGTFQLKK SGEKPLLGGS LMEYAILSAI
360 370 380 390 400
AAMNEPKTCS TTALKKYVLE NHPGTNSNYQ MHLLKKTLQK CEKNGWMEQI
410 420 430 440 450
SGKGFSGTFQ LCFPYYPSPG VLFPKKEPDD SRDEDEDEDE SSEEDSEDEE
460 470 480 490 500
PPPKRRLQKK TPAKSPGKAA SVKQRGSKPA PKVSAAQRGK ARPLPKKAPP
510 520 530 540 550
KAKTPAKKTR PSSTVIKKPS GGSSKKPATS ARKEVKLPGK GKSTMKKSFR

VKK
Length:553
Mass (Da):61,207
Last modified:December 21, 2004 - v1
Checksum:iD18B28EE0156F09B
GO
Isoform 2 (identifier: Q5SSJ5-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-38: Missing.

Show »
Length:515
Mass (Da):57,211
Checksum:i130AD6B3FFD97525
GO
Isoform 3 (identifier: Q5SSJ5-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-152: Missing.

Show »
Length:401
Mass (Da):44,435
Checksum:i31A31A118D2508B7
GO
Isoform 4 (identifier: Q5SSJ5-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     117-133: DEKDQSKEKEKKVKKTI → ERADSIHSTLFIIGQNS
     134-553: Missing.

Show »
Length:133
Mass (Da):14,594
Checksum:i87E1DE4A3A8FB907
GO

Sequence cautioni

The sequence AAF14871 differs from that shown. Reason: Frameshift at positions 226, 237 and 243.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti262V → G in AAF14871 (PubMed:10931946).Curated1
Sequence conflicti267F → S in BAF83941 (PubMed:14702039).Curated1
Sequence conflicti499P → S in BAG64972 (PubMed:14702039).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0341681 – 152Missing in isoform 3. 2 PublicationsAdd BLAST152
Alternative sequenceiVSP_0341691 – 38Missing in isoform 2. 1 PublicationAdd BLAST38
Alternative sequenceiVSP_034170117 – 133DEKDQ…VKKTI → ERADSIHSTLFIIGQNS in isoform 4. 1 PublicationAdd BLAST17
Alternative sequenceiVSP_034171134 – 553Missing in isoform 4. 1 PublicationAdd BLAST420

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF113534 mRNA Translation: AAF14871.1 Frameshift.
AK023129 mRNA Translation: BAG51160.1
AK291252 mRNA Translation: BAF83941.1
AK304065 mRNA Translation: BAG64972.1
AL833978 mRNA Translation: CAD38822.1
AL606477, AL663074 Genomic DNA Translation: CAI12528.1
AL606477, AL663074 Genomic DNA Translation: CAI12530.1
AL606477 Genomic DNA Translation: CAI12531.1
AL606477 Genomic DNA Translation: CAI12532.1
AL606477, AL663074 Genomic DNA Translation: CAI12533.1
AL663074, AL606477 Genomic DNA Translation: CAI23387.1
AL663074, AL606477 Genomic DNA Translation: CAI23388.1
AL663074, AL606477 Genomic DNA Translation: CAI23389.1
CH471134 Genomic DNA Translation: EAW94953.1
BC032139 mRNA Translation: AAH32139.1
BC045660 mRNA Translation: AAH45660.1
CCDSiCCDS30621.1 [Q5SSJ5-1]
RefSeqiNP_057371.2, NM_016287.3 [Q5SSJ5-1]
XP_005245932.1, XM_005245875.4 [Q5SSJ5-1]
XP_005245933.1, XM_005245876.3 [Q5SSJ5-1]
XP_005245934.1, XM_005245877.4 [Q5SSJ5-1]
XP_005245935.1, XM_005245878.4 [Q5SSJ5-1]
XP_005245936.1, XM_005245879.4 [Q5SSJ5-2]
XP_011539834.1, XM_011541532.1 [Q5SSJ5-2]
XP_011539835.1, XM_011541533.1 [Q5SSJ5-2]
XP_011539836.1, XM_011541534.2 [Q5SSJ5-2]
XP_016856882.1, XM_017001393.1 [Q5SSJ5-1]
XP_016856883.1, XM_017001394.1 [Q5SSJ5-2]
UniGeneiHs.142442

Genome annotation databases

EnsembliENST00000312239; ENSP00000312625; ENSG00000127483 [Q5SSJ5-1]
ENST00000375000; ENSP00000364139; ENSG00000127483 [Q5SSJ5-5]
ENST00000375003; ENSP00000364142; ENSG00000127483 [Q5SSJ5-3]
GeneIDi50809
KEGGihsa:50809
UCSCiuc001bdw.2 human [Q5SSJ5-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiHP1B3_HUMAN
AccessioniPrimary (citable) accession number: Q5SSJ5
Secondary accession number(s): A6NI71
, A8K5D7, B3KMZ8, B4E210, Q05BI0, Q5SSJ6, Q5SWC6, Q6PIM9, Q8NDF0, Q9UHY0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 10, 2008
Last sequence update: December 21, 2004
Last modified: April 25, 2018
This is version 136 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome
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Main funding by: National Institutes of Health