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Protein

Neuferricin

Gene

Cyb5d2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Heme-binding protein which promotes neuronal but not astrocyte differentiation.1 Publication

GO - Molecular functioni

  • heme binding Source: MGI

GO - Biological processi

  • positive regulation of neuron differentiation Source: MGI
Complete GO annotation...

Keywords - Biological processi

Neurogenesis

Names & Taxonomyi

Protein namesi
Recommended name:
Neuferricin
Alternative name(s):
Cytochrome b5 domain-containing protein 2
Gene namesi
Name:Cyb5d2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:2684848. Cyb5d2.

Subcellular locationi

GO - Cellular componenti

  • extracellular region Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2222Sequence analysisAdd
BLAST
Chaini23 – 263241NeuferricinPRO_0000312322Add
BLAST

Proteomic databases

MaxQBiQ5SSH8.
PaxDbiQ5SSH8.
PRIDEiQ5SSH8.

PTM databases

SwissPalmiQ5SSH8.

Expressioni

Tissue specificityi

Expressed in various tissues including brain, heart, adrenal gland, and kidney. In the brain, mainly expressed in pyramidal cells around the CA3 region of Ammon horn in hippocampus. Present in brain (at protein level).1 Publication

Developmental stagei

Abundantly expressed in the developing brain, in subventricular and ventricular zones of the cerebrum, where neural stem cells and neural precursor cells primarily exist. Expression levels gradually increase during brain development, between E14.5 and P49.1 Publication

Gene expression databases

BgeeiQ5SSH8.
CleanExiMM_CYB5D2.
GenevisibleiQ5SSH8. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000078623.

Structurei

3D structure databases

ProteinModelPortaliQ5SSH8.
SMRiQ5SSH8. Positions 38-134.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini35 – 134100Cytochrome b5 heme-bindingAdd
BLAST

Domaini

The cytochrome b5 heme-binding domain was proven to bind heme, although it lacks the conserved iron-binding His residues at position 73 and 106.

Sequence similaritiesi

Belongs to the cytochrome b5 family. MAPR subfamily.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG1108. Eukaryota.
ENOG4111JSU. LUCA.
GeneTreeiENSGT00730000110948.
HOGENOMiHOG000012760.
HOVERGENiHBG105428.
InParanoidiQ5SSH8.
OMAiGWWGPRA.
OrthoDBiEOG7HXCSZ.
PhylomeDBiQ5SSH8.
TreeFamiTF313943.

Family and domain databases

Gene3Di3.10.120.10. 1 hit.
InterProiIPR001199. Cyt_B5-like_heme/steroid-bd.
[Graphical view]
PfamiPF00173. Cyt-b5. 1 hit.
[Graphical view]
SMARTiSM01117. Cyt-b5. 1 hit.
[Graphical view]
SUPFAMiSSF55856. SSF55856. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q5SSH8-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MLRICGLGVV LSLAVAAVAV MAVWLMDWWG PRPGIRLFLP EELARYRGGP
60 70 80 90 100
GDPGLYLALL GRVYDVSSGR RHYEPGAHYS GFAGRDASRA FVTGDYSEAG
110 120 130 140 150
LVDDINGLSS SEILTLHNWL SFYEKNYVFV GRLVGRFYRK DGLPTSELTQ
160 170 180 190 200
VEAMVTKGME ANEQEQREKQ KFPPCNSEWS SAKGSRLWCS QKSGGVHRDW
210 220 230 240 250
IGVPRKLYKP GAKEPHCVCV RTTGPPSDQQ DNPRHSNHGD LDNPNLEEYT
260
GCPPLATTCS FPL
Length:263
Mass (Da):29,162
Last modified:December 21, 2004 - v1
Checksum:i41156B54DBF3218E
GO
Isoform 2 (identifier: Q5SSH8-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-153: Missing.

Show »
Length:110
Mass (Da):12,293
Checksum:i7EA4AB237CD40115
GO

Sequence cautioni

The sequence BAC37998.1 differs from that shown.Probable cloning artifact.Curated
The sequence BAE23849.1 differs from that shown.Probable cloning artifact.Curated
The sequence BAE25095.1 differs from that shown.Probable cloning artifact.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti76 – 761G → V in AAH86682 (PubMed:15489334).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 153153Missing in isoform 2. 1 PublicationVSP_029827Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL663082 Genomic DNA. Translation: CAI25189.1.
BC086682 mRNA. Translation: AAH86682.1.
BC117721 mRNA. Translation: AAI17722.2.
AK080730 mRNA. Translation: BAC37998.1. Sequence problems.
AK138991 mRNA. Translation: BAE23849.1. Sequence problems.
AK142515 mRNA. Translation: BAE25095.1. Sequence problems.
CCDSiCCDS24990.2. [Q5SSH8-1]
RefSeqiNP_001020097.2. NM_001024926.3. [Q5SSH8-1]
UniGeneiMm.194755.

Genome annotation databases

EnsembliENSMUST00000079681; ENSMUSP00000078623; ENSMUSG00000057778. [Q5SSH8-1]
ENSMUST00000156294; ENSMUSP00000131961; ENSMUSG00000057778. [Q5SSH8-2]
GeneIDi192986.
KEGGimmu:192986.
UCSCiuc007jzi.2. mouse. [Q5SSH8-1]
uc007jzj.2. mouse. [Q5SSH8-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL663082 Genomic DNA. Translation: CAI25189.1.
BC086682 mRNA. Translation: AAH86682.1.
BC117721 mRNA. Translation: AAI17722.2.
AK080730 mRNA. Translation: BAC37998.1. Sequence problems.
AK138991 mRNA. Translation: BAE23849.1. Sequence problems.
AK142515 mRNA. Translation: BAE25095.1. Sequence problems.
CCDSiCCDS24990.2. [Q5SSH8-1]
RefSeqiNP_001020097.2. NM_001024926.3. [Q5SSH8-1]
UniGeneiMm.194755.

3D structure databases

ProteinModelPortaliQ5SSH8.
SMRiQ5SSH8. Positions 38-134.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000078623.

PTM databases

SwissPalmiQ5SSH8.

Proteomic databases

MaxQBiQ5SSH8.
PaxDbiQ5SSH8.
PRIDEiQ5SSH8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000079681; ENSMUSP00000078623; ENSMUSG00000057778. [Q5SSH8-1]
ENSMUST00000156294; ENSMUSP00000131961; ENSMUSG00000057778. [Q5SSH8-2]
GeneIDi192986.
KEGGimmu:192986.
UCSCiuc007jzi.2. mouse. [Q5SSH8-1]
uc007jzj.2. mouse. [Q5SSH8-2]

Organism-specific databases

CTDi124936.
MGIiMGI:2684848. Cyb5d2.

Phylogenomic databases

eggNOGiKOG1108. Eukaryota.
ENOG4111JSU. LUCA.
GeneTreeiENSGT00730000110948.
HOGENOMiHOG000012760.
HOVERGENiHBG105428.
InParanoidiQ5SSH8.
OMAiGWWGPRA.
OrthoDBiEOG7HXCSZ.
PhylomeDBiQ5SSH8.
TreeFamiTF313943.

Miscellaneous databases

PROiQ5SSH8.
SOURCEiSearch...

Gene expression databases

BgeeiQ5SSH8.
CleanExiMM_CYB5D2.
GenevisibleiQ5SSH8. MM.

Family and domain databases

Gene3Di3.10.120.10. 1 hit.
InterProiIPR001199. Cyt_B5-like_heme/steroid-bd.
[Graphical view]
PfamiPF00173. Cyt-b5. 1 hit.
[Graphical view]
SMARTiSM01117. Cyt-b5. 1 hit.
[Graphical view]
SUPFAMiSSF55856. SSF55856. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Strain: C57BL/6J.
    Tissue: Eye.
  3. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-84 (ISOFORM 1).
    Strain: C57BL/6J.
    Tissue: Blood vessel, Kidney and Retina.
  4. "Neuferricin, a novel extracellular heme-binding protein, promotes neurogenesis."
    Kimura I., Nakayama Y., Konishi M., Kobayashi T., Mori M., Ito M., Hirasawa A., Tsujimoto G., Ohta M., Itoh N., Fujimoto M.
    J. Neurochem. 112:1156-1167(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: HEME-BINDING, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, SUBCELLULAR LOCATION, FUNCTION.

Entry informationi

Entry nameiNEUFC_MOUSE
AccessioniPrimary (citable) accession number: Q5SSH8
Secondary accession number(s): A0AUN6, Q0VGS6, Q8BHV3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 4, 2007
Last sequence update: December 21, 2004
Last modified: June 8, 2016
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.