Q5SRI9 (MANEA_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glycoprotein endo-alpha-1,2-mannosidase
Short name=Endo-alpha mannosidase
Short name=Endomannosidase
Short name=hEndo
EC=3.2.1.130

Alternative name(s):
Mandaselin

Gene names

Name:

MANEA

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	462 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	Hydrolysis of the terminal alpha-D-glucosyl-(1,3)-D-mannosyl unit from the GlcMan9(GlcNAc)2 oligosaccharide component of the glycoprotein produced in the Golgi membrane. Ref.1 Ref.2
Subcellular location	Golgi apparatus membrane; Single-pass type II membrane protein Ref.1 Ref.2.
Tissue specificity	Highly expressed in the liver and kidney. Expressed at lower levels in muscle, pancreas, heart, placenta, lung and brain. Ref.2
Post-translational modification	Undergoes proteolytic cleavage in the C-terminal region.
Sequence similarities	Belongs to the glycosyl hydrolase 99 family.
Biophysicochemical properties	pH dependence: Optimum pH is 6.2. Ref.2 Temperature dependence: Optimum temperature is 37 degrees Celsius.
Sequence caution	The sequence AAL07306.1 differs from that shown. Reason: Frameshift at several positions. The sequence CAD97640.1 differs from that shown. Reason: Erroneous initiation. The sequence CAI17346.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Coding sequence diversity	Polymorphism
Domain	Signal-anchor Transmembrane Transmembrane helix
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	post-translational protein modification Traceable author statement. Source: Reactome protein N-linked glycosylation via asparagine Traceable author statement. Source: Reactome
Cellular_component	Golgi membrane Traceable author statement. Source: Reactome integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	glycoprotein endo-alpha-1,2-mannosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 462	462	Glycoprotein endo-alpha-1,2-mannosidase		PRO_0000282315
Regions
	Topological domain	1 – 8	8	Cytoplasmic Potential
	Transmembrane	9 – 29	21	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	30 – 462	433	Lumenal Potential
	Region	60 – 462	403	Catalytic Probable
Natural variations
	Natural variant	331	1	Y → C in a breast cancer sample; somatic mutation. Ref.8		VAR_036242
Experimental info
	Sequence conflict	129	1	E → G in CAD97640. Ref.4
	Sequence conflict	237	1	N → S in CAE12165. Ref.1
	Sequence conflict	344	1	F → I in AAQ75077. Ref.2
	Sequence conflict	344	1	F → I in BAB14298. Ref.3
	Sequence conflict	344	1	F → I in CAE45927. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Q5SRI9 [UniParc]. Last modified December 21, 2004. Version 1. Checksum: CB593CB9B3DAA056	FASTA	462	53,671
	10 20 30 40 50 60 MAKFRRRTCI ILALFILFIF SLMMGLKMLR PNTATFGAPF GLDLLPELHQ RTIHLGKNFD 70 80 90 100 110 120 FQKSDRINSE TNTKNLKSVE ITMKPSKASE LNLDELPPLN NYLHVFYYSW YGNPQFDGKY 130 140 150 160 170 180 IHWNHPVLEH WDPRIAKNYP QGRHNPPDDI GSSFYPELGS YSSRDPSVIE THMRQMRSAS 190 200 210 220 230 240 IGVLALSWYP PDVNDENGEP TDNLVPTILD KAHKYNLKVT FHIEPYSNRD DQNMYKNVKY 250 260 270 280 290 300 IIDKYGNHPA FYRYKTKTGN ALPMFYVYDS YITKPEKWAN LLTTSGSRSI RNSPYDGLFI 310 320 330 340 350 360 ALLVEEKHKY DILQSGFDGI YTYFATNGFT YGSSHQNWAS LKLFCDKYNL IFIPSVGPGY 370 380 390 400 410 420 IDTSIRPWNT QNTRNRINGK YYEIGLSAAL QTRPSLISIT SFNEWHEGTQ IEKAVPKRTS 430 440 450 460 NTVYLDYRPH KPGLYLELTR KWSEKYSKER ATYALDRQLP VS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Human endo-alpha1,2-mannosidase is a Golgi-resident type II membrane protein." Hardt B., Voelker C., Mundt S., Salska-Navarro M., Hauptmann M., Bause E. Biochimie 87:169-179(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, TOPOLOGY, CATALYTIC ACTIVITY.
[2]	"Intact alpha-1,2-endomannosidase is a typical type II membrane protein." Hamilton S.R., Li H., Wischnewski H., Prasad A., Kerley-Hamilton J.S., Mitchell T., Walling A.J., Davidson R.C., Wildt S., Gerngross T.U. Glycobiology 15:615-624(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY, PTM, SUBCELLULAR LOCATION, TOPOLOGY. Tissue: Liver.
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Fetal kidney.
[5]	"The DNA sequence and analysis of human chromosome 6." Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L., Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R., Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D., Andrews T.D. , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H., Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V., Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J., Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E., Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J., French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J., Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C., Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A., Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R., Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M., Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R., Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A., Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L., Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I., Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y., Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E., Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A., Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W., Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J., Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M., Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I., Rogers J., Beck S. Nature 425:805-811(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[7]	"Novel gene expressed in human liver." Zeng L., Liu F., Xu X., Zhang X., Chen Z., Han Z. Submitted (JUL-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-214. Tissue: Liver.
[8]	"The consensus coding sequences of human breast and colorectal cancers." Sjoeblom T., Jones S., Wood L.D., Parsons D.W., Lin J., Barber T.D., Mandelker D., Leary R.J., Ptak J., Silliman N., Szabo S., Buckhaults P., Farrell C., Meeh P., Markowitz S.D., Willis J., Dawson D., Willson J.K.V. , Gazdar A.F., Hartigan J., Wu L., Liu C., Parmigiani G., Park B.H., Bachman K.E., Papadopoulos N., Vogelstein B., Kinzler K.W., Velculescu V.E. Science 314:268-274(2006) [PubMed] [Europe PMC] [Abstract] Cited for: VARIANT [LARGE SCALE ANALYSIS] CYS-331.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ577574 mRNA. Translation: CAE12165.1. AY372528 mRNA. Translation: AAQ75077.1. AK022900 mRNA. Translation: BAB14298.1. AK291940 mRNA. Translation: BAF84629.1. BX537398 mRNA. Translation: CAD97640.1. Different initiation. BX640869 mRNA. Translation: CAE45927.1. AL671884 Genomic DNA. Translation: CAI17346.1. Sequence problems. AL671884 Genomic DNA. Translation: CAI17347.1. BC137014 mRNA. Translation: AAI37015.1. BC137016 mRNA. Translation: AAI37017.1. BC146671 mRNA. Translation: AAI46672.1. BC150199 mRNA. Translation: AAI50200.1. AY048774 mRNA. Translation: AAL07306.1. Frameshift.
IPI	IPI00478860.
RefSeq	NP_078917.2. NM_024641.3.
UniGene	Hs.734632. Hs.743412.
3D structure databases
ProteinModelPortal	Q5SRI9.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000351669.
Protein family/group databases
CAZy	GH99. Glycoside Hydrolase Family 99.
PTM databases
PhosphoSite	Q5SRI9.
Polymorphism databases
DMDM	74743637.
Proteomic databases
PaxDb	Q5SRI9.
PRIDE	Q5SRI9.
Protocols and materials databases
DNASU	79694.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000358812; ENSP00000351669; ENSG00000172469. ENST00000369293; ENSP00000358299; ENSG00000172469.
GeneID	79694.
KEGG	hsa:79694.
UCSC	uc003poo.2. human.
Organism-specific databases
CTD	79694.
GeneCards	GC06P096071.
HGNC	HGNC:21072. MANEA.
HPA	HPA011046. HPA011069.
MIM	612327. gene.
neXtProt	NX_Q5SRI9.
PharmGKB	PA134891001.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG84309.
HOVERGEN	HBG103563.
InParanoid	Q5SRI9.
KO	K15538.
OMA	IETHMKQ.
OrthoDB	EOG44MXS2.
Enzyme and pathway databases
Reactome	REACT_17015. Metabolism of proteins.
Gene expression databases
Bgee	Q5SRI9.
CleanEx	HS_MANEA.
Genevestigator	Q5SRI9.
Family and domain databases
InterPro	IPR026071. Glyco_Hydrolase_99. IPR017853. Glycoside_hydrolase_SF. [Graphical view]
PANTHER	PTHR13572. PTHR13572. 1 hit.
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit.
ProtoNet	Search...
Other
GenomeRNAi	79694.
NextBio	68976.
SOURCE	Search...

Entry information

Entry name

MANEA_HUMAN

Accession

Primary (citable) accession number: Q5SRI9
Secondary accession number(s): A6H8M6 , Q5SRJ0, Q6MZV0, Q70JE9, Q7Z3V7, Q8WWX5, Q9H9D2

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 3, 2007
Last sequence update:	December 21, 2004
Last modified:	May 29, 2013
This is version 82 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.