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Protein

Glycoprotein endo-alpha-1,2-mannosidase

Gene

MANEA

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Hydrolysis of the terminal alpha-D-glucosyl-(1,3)-D-mannosyl unit from the GlcMan9(GlcNAc)2 oligosaccharide component of the glycoprotein produced in the Golgi membrane.2 Publications

pH dependencei

Optimum pH is 6.2.1 Publication

Temperature dependencei

Optimum temperature is 37 degrees Celsius.1 Publication

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Enzyme and pathway databases

BRENDAi3.2.1.130. 2681.
ReactomeiR-HSA-964739. N-glycan trimming and elongation in the cis-Golgi.

Protein family/group databases

CAZyiGH99. Glycoside Hydrolase Family 99.

Names & Taxonomyi

Protein namesi
Recommended name:
Glycoprotein endo-alpha-1,2-mannosidase (EC:3.2.1.130)
Short name:
Endo-alpha mannosidase
Short name:
Endomannosidase
Short name:
hEndo
Alternative name(s):
Mandaselin
Gene namesi
Name:MANEA
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 6

Organism-specific databases

HGNCiHGNC:21072. MANEA.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 88CytoplasmicSequence analysis
Transmembranei9 – 2921Helical; Signal-anchor for type II membrane proteinSequence analysisAdd
BLAST
Topological domaini30 – 462433LumenalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA134891001.

Polymorphism and mutation databases

BioMutaiMANEA.
DMDMi74743637.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 462462Glycoprotein endo-alpha-1,2-mannosidasePRO_0000282315Add
BLAST

Post-translational modificationi

Undergoes proteolytic cleavage in the C-terminal region.

Proteomic databases

EPDiQ5SRI9.
MaxQBiQ5SRI9.
PaxDbiQ5SRI9.
PRIDEiQ5SRI9.

PTM databases

iPTMnetiQ5SRI9.
PhosphoSiteiQ5SRI9.
SwissPalmiQ5SRI9.

Expressioni

Tissue specificityi

Highly expressed in the liver and kidney. Expressed at lower levels in muscle, pancreas, heart, placenta, lung and brain.1 Publication

Gene expression databases

BgeeiQ5SRI9.
CleanExiHS_MANEA.
ExpressionAtlasiQ5SRI9. baseline and differential.
GenevisibleiQ5SRI9. HS.

Organism-specific databases

HPAiHPA011046.
HPA011069.

Interactioni

Protein-protein interaction databases

BioGridi122815. 25 interactions.
STRINGi9606.ENSP00000351669.

Structurei

3D structure databases

ProteinModelPortaliQ5SRI9.
SMRiQ5SRI9. Positions 99-451.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni60 – 462403CatalyticCuratedAdd
BLAST

Sequence similaritiesi

Belongs to the glycosyl hydrolase 99 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IG22. Eukaryota.
ENOG410XT8Q. LUCA.
GeneTreeiENSGT00390000016054.
HOVERGENiHBG103563.
InParanoidiQ5SRI9.
KOiK15538.
OMAiIETHMKQ.
OrthoDBiEOG7CVQ05.
PhylomeDBiQ5SRI9.
TreeFamiTF324051.

Family and domain databases

InterProiIPR026071. Glyco_Hydrolase_99.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR13572. PTHR13572. 1 hit.
PfamiPF16317. Glyco_hydro_99. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.

Sequencei

Sequence statusi: Complete.

Q5SRI9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKFRRRTCI ILALFILFIF SLMMGLKMLR PNTATFGAPF GLDLLPELHQ
60 70 80 90 100
RTIHLGKNFD FQKSDRINSE TNTKNLKSVE ITMKPSKASE LNLDELPPLN
110 120 130 140 150
NYLHVFYYSW YGNPQFDGKY IHWNHPVLEH WDPRIAKNYP QGRHNPPDDI
160 170 180 190 200
GSSFYPELGS YSSRDPSVIE THMRQMRSAS IGVLALSWYP PDVNDENGEP
210 220 230 240 250
TDNLVPTILD KAHKYNLKVT FHIEPYSNRD DQNMYKNVKY IIDKYGNHPA
260 270 280 290 300
FYRYKTKTGN ALPMFYVYDS YITKPEKWAN LLTTSGSRSI RNSPYDGLFI
310 320 330 340 350
ALLVEEKHKY DILQSGFDGI YTYFATNGFT YGSSHQNWAS LKLFCDKYNL
360 370 380 390 400
IFIPSVGPGY IDTSIRPWNT QNTRNRINGK YYEIGLSAAL QTRPSLISIT
410 420 430 440 450
SFNEWHEGTQ IEKAVPKRTS NTVYLDYRPH KPGLYLELTR KWSEKYSKER
460
ATYALDRQLP VS
Length:462
Mass (Da):53,671
Last modified:December 21, 2004 - v1
Checksum:iCB593CB9B3DAA056
GO

Sequence cautioni

The sequence AAL07306.1 differs from that shown. Reason: Frameshift at several positions. Curated
The sequence CAD97640.1 differs from that shown. Reason: Erroneous initiation. Curated
The sequence CAI17346.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti129 – 1291E → G in CAD97640 (PubMed:17974005).Curated
Sequence conflicti237 – 2371N → S in CAE12165 (PubMed:15760709).Curated
Sequence conflicti344 – 3441F → I in AAQ75077 (PubMed:15677381).Curated
Sequence conflicti344 – 3441F → I in BAB14298 (PubMed:14702039).Curated
Sequence conflicti344 – 3441F → I in CAE45927 (PubMed:17974005).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti331 – 3311Y → C in a breast cancer sample; somatic mutation. 1 Publication
VAR_036242

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ577574 mRNA. Translation: CAE12165.1.
AY372528 mRNA. Translation: AAQ75077.1.
AK022900 mRNA. Translation: BAB14298.1.
AK291940 mRNA. Translation: BAF84629.1.
BX537398 mRNA. Translation: CAD97640.1. Different initiation.
BX640869 mRNA. Translation: CAE45927.1.
AL671884 Genomic DNA. Translation: CAI17346.1. Sequence problems.
AL671884 Genomic DNA. Translation: CAI17347.1.
BC137014 mRNA. Translation: AAI37015.1.
BC137016 mRNA. Translation: AAI37017.1.
BC146671 mRNA. Translation: AAI46672.1.
BC150199 mRNA. Translation: AAI50200.1.
AY048774 mRNA. Translation: AAL07306.1. Frameshift.
CCDSiCCDS5032.1.
RefSeqiNP_078917.2. NM_024641.3.
XP_005267204.1. XM_005267147.2.
UniGeneiHs.734632.
Hs.743412.

Genome annotation databases

EnsembliENST00000358812; ENSP00000351669; ENSG00000172469.
GeneIDi79694.
KEGGihsa:79694.
UCSCiuc003poo.3. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ577574 mRNA. Translation: CAE12165.1.
AY372528 mRNA. Translation: AAQ75077.1.
AK022900 mRNA. Translation: BAB14298.1.
AK291940 mRNA. Translation: BAF84629.1.
BX537398 mRNA. Translation: CAD97640.1. Different initiation.
BX640869 mRNA. Translation: CAE45927.1.
AL671884 Genomic DNA. Translation: CAI17346.1. Sequence problems.
AL671884 Genomic DNA. Translation: CAI17347.1.
BC137014 mRNA. Translation: AAI37015.1.
BC137016 mRNA. Translation: AAI37017.1.
BC146671 mRNA. Translation: AAI46672.1.
BC150199 mRNA. Translation: AAI50200.1.
AY048774 mRNA. Translation: AAL07306.1. Frameshift.
CCDSiCCDS5032.1.
RefSeqiNP_078917.2. NM_024641.3.
XP_005267204.1. XM_005267147.2.
UniGeneiHs.734632.
Hs.743412.

3D structure databases

ProteinModelPortaliQ5SRI9.
SMRiQ5SRI9. Positions 99-451.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi122815. 25 interactions.
STRINGi9606.ENSP00000351669.

Protein family/group databases

CAZyiGH99. Glycoside Hydrolase Family 99.

PTM databases

iPTMnetiQ5SRI9.
PhosphoSiteiQ5SRI9.
SwissPalmiQ5SRI9.

Polymorphism and mutation databases

BioMutaiMANEA.
DMDMi74743637.

Proteomic databases

EPDiQ5SRI9.
MaxQBiQ5SRI9.
PaxDbiQ5SRI9.
PRIDEiQ5SRI9.

Protocols and materials databases

DNASUi79694.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000358812; ENSP00000351669; ENSG00000172469.
GeneIDi79694.
KEGGihsa:79694.
UCSCiuc003poo.3. human.

Organism-specific databases

CTDi79694.
GeneCardsiMANEA.
HGNCiHGNC:21072. MANEA.
HPAiHPA011046.
HPA011069.
MIMi612327. gene.
neXtProtiNX_Q5SRI9.
PharmGKBiPA134891001.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IG22. Eukaryota.
ENOG410XT8Q. LUCA.
GeneTreeiENSGT00390000016054.
HOVERGENiHBG103563.
InParanoidiQ5SRI9.
KOiK15538.
OMAiIETHMKQ.
OrthoDBiEOG7CVQ05.
PhylomeDBiQ5SRI9.
TreeFamiTF324051.

Enzyme and pathway databases

BRENDAi3.2.1.130. 2681.
ReactomeiR-HSA-964739. N-glycan trimming and elongation in the cis-Golgi.

Miscellaneous databases

GeneWikiiMANEA.
GenomeRNAii79694.
PROiQ5SRI9.
SOURCEiSearch...

Gene expression databases

BgeeiQ5SRI9.
CleanExiHS_MANEA.
ExpressionAtlasiQ5SRI9. baseline and differential.
GenevisibleiQ5SRI9. HS.

Family and domain databases

InterProiIPR026071. Glyco_Hydrolase_99.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR13572. PTHR13572. 1 hit.
PfamiPF16317. Glyco_hydro_99. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Human endo-alpha1,2-mannosidase is a Golgi-resident type II membrane protein."
    Hardt B., Voelker C., Mundt S., Salska-Navarro M., Hauptmann M., Bause E.
    Biochimie 87:169-179(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, TOPOLOGY, CATALYTIC ACTIVITY.
  2. Cited for: NUCLEOTIDE SEQUENCE [MRNA], CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY, PTM, SUBCELLULAR LOCATION, TOPOLOGY.
    Tissue: Liver.
  3. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Fetal kidney.
  5. "The DNA sequence and analysis of human chromosome 6."
    Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L., Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R., Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D., Andrews T.D.
    , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H., Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V., Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J., Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E., Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J., French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J., Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C., Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A., Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R., Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M., Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R., Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A., Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L., Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I., Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y., Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E., Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A., Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W., Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J., Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M., Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I., Rogers J., Beck S.
    Nature 425:805-811(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  7. "Novel gene expressed in human liver."
    Zeng L., Liu F., Xu X., Zhang X., Chen Z., Han Z.
    Submitted (JUL-2001) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-214.
    Tissue: Liver.
  8. Cited for: VARIANT [LARGE SCALE ANALYSIS] CYS-331.

Entry informationi

Entry nameiMANEA_HUMAN
AccessioniPrimary (citable) accession number: Q5SRI9
Secondary accession number(s): A6H8M6
, Q5SRJ0, Q6MZV0, Q70JE9, Q7Z3V7, Q8WWX5, Q9H9D2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 3, 2007
Last sequence update: December 21, 2004
Last modified: June 8, 2016
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. Human chromosome 6
    Human chromosome 6: entries, gene names and cross-references to MIM
  3. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  4. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  5. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.