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Q5SNX7 (GALC_DANRE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 62. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Galactocerebrosidase

Short name=GALCERase
EC=3.2.1.46
Alternative name(s):
Galactosylceramidase
Gene names
Name:galc
Synonyms:galca
ORF Names:si:ch211-199l3.4, zgc:92561
OrganismDanio rerio (Zebrafish) (Brachydanio rerio) [Reference proteome]
Taxonomic identifier7955 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio

Protein attributes

Sequence length660 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Hydrolyzes the galactose ester bonds of galactosylceramide, galactosylsphingosine, lactosylceramide, and monogalactosyldiglyceride By similarity.

Catalytic activity

D-galactosyl-N-acylsphingosine + H2O = D-galactose + N-acylsphingosine.

Subcellular location

Lysosome By similarity.

Sequence similarities

Belongs to the glycosyl hydrolase 59 family.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1818 Potential
Chain19 – 660642Galactocerebrosidase
PRO_0000370713

Sites

Active site1761Proton donor/acceptor By similarity
Active site2511Nucleophile By similarity
Binding site871Substrate By similarity
Binding site1291Substrate By similarity
Binding site1751Substrate By similarity
Binding site3731Substrate By similarity

Amino acid modifications

Glycosylation1471N-linked (GlcNAc...) Potential
Glycosylation2931N-linked (GlcNAc...) Potential
Glycosylation3561N-linked (GlcNAc...) Potential
Glycosylation4131N-linked (GlcNAc...) Potential
Glycosylation4651N-linked (GlcNAc...) Potential
Glycosylation4951N-linked (GlcNAc...) Potential
Glycosylation4991N-linked (GlcNAc...) Potential
Glycosylation5371N-linked (GlcNAc...) Potential
Glycosylation5781N-linked (GlcNAc...) Potential
Disulfide bond264 ↔ 371 By similarity

Experimental info

Sequence conflict111S → F in AAH83517. Ref.2
Sequence conflict1901A → V in AAH83517. Ref.2
Sequence conflict4181K → R in AAH83517. Ref.2
Sequence conflict5111T → I in AAH83517. Ref.2
Sequence conflict5911R → G in AAH83517. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Q5SNX7 [UniParc].

Last modified December 21, 2004. Version 1.
Checksum: AC70CBB2903A9B77

FASTA66073,820
        10         20         30         40         50         60 
MQTHNFLCII SVILGCSAQF YVIDDRIGLG REFDGIGGLS GGGATSRLLV NYEEPYRSQI 

        70         80         90        100        110        120 
LDYLFKPNFG ASLHILKVEI GGDAQTTDGT EPSHMHSKDE GNFFRGYEWW LMKEAKKRNP 

       130        140        150        160        170        180 
NIKLIGLPWA FPGWVGYGTQ WPYFFPNVTA NYVITWVMGA KQHHNLDIDY IGIWNEKAFD 

       190        200        210        220        230        240 
PTYIKVLRDA LDRAGFTNIG IIAADGDWSI ASAMLDDPYL NDAVEVIGVH YPGTNTVKEA 

       250        260        270        280        290        300 
LLTERKLWSS EDYSTYNDDI GAGCWARILN QNYVNGKMTS TISWNVIASY YENLSFGRDG 

       310        320        330        340        350        360 
LMTAEEPWSG HYIVESPIWM TAHTTQFTQP GWFYLQTVGK LNHGGSYVAL TDRKGNLTII 

       370        380        390        400        410        420 
IETMTHEHSQ CIRPPLPHFD VSPQIATFEL KGSFAHLADL QVWYSKLDFK SGNGTLFKQL 

       430        440        450        460        470        480 
RPIRAHNGLL SLKLDVDEVF TITTVTTAQR GFYPEPPKSC PFPKNYTDDF TIDNPPFSEA 

       490        500        510        520        530        540 
PYFADQTGVF EYFRNTTDNS SHAFTLRQVV TERPVAWAKD ADQTISIIGD YSWSDVNVSC 

       550        560        570        580        590        600 
DVFIETPKTG GVFLAARVDQ GGESVRQAKG VFYWIYANGT YRVTNDIGGK RVLAEGLSGT 

       610        620        630        640        650        660 
KAGVWYTLTL SVKGYFATGS LNGFPLWKNA AVLEPKSGWA ALGTLSFEYA QFDNFNVIAG 

« Hide

References

[1]"The zebrafish reference genome sequence and its relationship to the human genome."
Howe K., Clark M.D., Torroja C.F., Torrance J., Berthelot C., Muffato M., Collins J.E., Humphray S., McLaren K., Matthews L., McLaren S., Sealy I., Caccamo M., Churcher C., Scott C., Barrett J.C., Koch R., Rauch G.J. expand/collapse author list , White S., Chow W., Kilian B., Quintais L.T., Guerra-Assuncao J.A., Zhou Y., Gu Y., Yen J., Vogel J.H., Eyre T., Redmond S., Banerjee R., Chi J., Fu B., Langley E., Maguire S.F., Laird G.K., Lloyd D., Kenyon E., Donaldson S., Sehra H., Almeida-King J., Loveland J., Trevanion S., Jones M., Quail M., Willey D., Hunt A., Burton J., Sims S., McLay K., Plumb B., Davis J., Clee C., Oliver K., Clark R., Riddle C., Eliott D., Threadgold G., Harden G., Ware D., Mortimer B., Kerry G., Heath P., Phillimore B., Tracey A., Corby N., Dunn M., Johnson C., Wood J., Clark S., Pelan S., Griffiths G., Smith M., Glithero R., Howden P., Barker N., Stevens C., Harley J., Holt K., Panagiotidis G., Lovell J., Beasley H., Henderson C., Gordon D., Auger K., Wright D., Collins J., Raisen C., Dyer L., Leung K., Robertson L., Ambridge K., Leongamornlert D., McGuire S., Gilderthorp R., Griffiths C., Manthravadi D., Nichol S., Barker G., Whitehead S., Kay M., Brown J., Murnane C., Gray E., Humphries M., Sycamore N., Barker D., Saunders D., Wallis J., Babbage A., Hammond S., Mashreghi-Mohammadi M., Barr L., Martin S., Wray P., Ellington A., Matthews N., Ellwood M., Woodmansey R., Clark G., Cooper J., Tromans A., Grafham D., Skuce C., Pandian R., Andrews R., Harrison E., Kimberley A., Garnett J., Fosker N., Hall R., Garner P., Kelly D., Bird C., Palmer S., Gehring I., Berger A., Dooley C.M., Ersan-Urun Z., Eser C., Geiger H., Geisler M., Karotki L., Kirn A., Konantz J., Konantz M., Oberlander M., Rudolph-Geiger S., Teucke M., Osoegawa K., Zhu B., Rapp A., Widaa S., Langford C., Yang F., Carter N.P., Harrow J., Ning Z., Herrero J., Searle S.M., Enright A., Geisler R., Plasterk R.H., Lee C., Westerfield M., de Jong P.J., Zon L.I., Postlethwait J.H., Nusslein-Volhard C., Hubbard T.J., Roest Crollius H., Rogers J., Stemple D.L.
Nature 496:498-503(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Tuebingen.
[2]NIH - Zebrafish Gene Collection (ZGC) project
Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: AB.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AL953887 Genomic DNA. Translation: CAI11729.1.
BC083517 mRNA. Translation: AAH83517.1.
RefSeqNP_001005921.1. NM_001005921.1.
UniGeneDr.106140.

3D structure databases

ProteinModelPortalQ5SNX7.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING7955.ENSDARP00000053869.

Protein family/group databases

CAZyGH59. Glycoside Hydrolase Family 59.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSDART00000053870; ENSDARP00000053869; ENSDARG00000037064.
GeneID449649.
KEGGdre:449649.

Organism-specific databases

CTD449649.
ZFINZDB-GENE-040724-243. galca.

Phylogenomic databases

eggNOGNOG76999.
GeneTreeENSGT00390000003303.
HOGENOMHOG000068033.
InParanoidQ5XJ01.
KOK01202.
OMAIDYPEPE.
OrthoDBEOG77WWCH.
PhylomeDBQ5SNX7.
TreeFamTF312985.

Gene expression databases

BgeeQ5SNX7.

Family and domain databases

Gene3D3.20.20.80. 1 hit.
InterProIPR001286. Glyco_hydro_59.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERPTHR15172. PTHR15172. 1 hit.
PfamPF02057. Glyco_hydro_59. 1 hit.
[Graphical view]
PRINTSPR00850. GLHYDRLASE59.
SUPFAMSSF51445. SSF51445. 1 hit.
ProtoNetSearch...

Other

NextBio20832750.

Entry information

Entry nameGALC_DANRE
AccessionPrimary (citable) accession number: Q5SNX7
Secondary accession number(s): Q5XJ01
Entry history
Integrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: December 21, 2004
Last modified: April 16, 2014
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries