Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Galactocerebrosidase

Gene

galc

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Hydrolyzes the galactose ester bonds of galactosylceramide, galactosylsphingosine, lactosylceramide, and monogalactosyldiglyceride.By similarity

Catalytic activityi

D-galactosyl-N-acylsphingosine + H2O = D-galactose + N-acylsphingosine.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei87SubstrateBy similarity1
Binding sitei129SubstrateBy similarity1
Binding sitei175SubstrateBy similarity1
Active sitei176Proton donor/acceptorBy similarity1
Active sitei251NucleophileBy similarity1
Binding sitei373SubstrateBy similarity1

GO - Molecular functioni

  • galactosylceramidase activity Source: ZFIN

GO - Biological processi

Keywordsi

Molecular functionGlycosidase, Hydrolase
Biological processLipid degradation, Lipid metabolism, Sphingolipid metabolism

Enzyme and pathway databases

ReactomeiR-DRE-1660662 Glycosphingolipid metabolism

Protein family/group databases

CAZyiGH59 Glycoside Hydrolase Family 59

Names & Taxonomyi

Protein namesi
Recommended name:
Galactocerebrosidase (EC:3.2.1.46)
Short name:
GALCERase
Alternative name(s):
Galactosylceramidase
Gene namesi
Name:galc
Synonyms:galca
ORF Names:si:ch211-199l3.4, zgc:92561
OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
Taxonomic identifieri7955 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
Proteomesi
  • UP000000437 Componenti: Chromosome 20

Organism-specific databases

ZFINiZDB-GENE-040724-243 galca

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Lysosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 18Sequence analysisAdd BLAST18
ChainiPRO_000037071319 – 660GalactocerebrosidaseAdd BLAST642

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi147N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi264 ↔ 371By similarity
Glycosylationi293N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi356N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi413N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi465N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi495N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi499N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi537N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi578N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ5SNX7
PRIDEiQ5SNX7

Expressioni

Gene expression databases

BgeeiENSDARG00000037064

Interactioni

Protein-protein interaction databases

STRINGi7955.ENSDARP00000053869

Structurei

3D structure databases

ProteinModelPortaliQ5SNX7
SMRiQ5SNX7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 59 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IITE Eukaryota
ENOG410XTIS LUCA
GeneTreeiENSGT00390000003303
HOGENOMiHOG000068033
InParanoidiQ5SNX7
KOiK01202
OMAiHEAWDEN
OrthoDBiEOG091G04GY
PhylomeDBiQ5SNX7
TreeFamiTF312985

Family and domain databases

Gene3Di3.20.20.70, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR001286 Glyco_hydro_59
IPR035394 Glyco_hydro_59_dom
IPR017853 Glycoside_hydrolase_SF
PANTHERiPTHR15172 PTHR15172, 1 hit
PfamiView protein in Pfam
PF02057 Glyco_hydro_59, 1 hit
PF17387 Glyco_hydro_59M, 1 hit
PRINTSiPR00850 GLHYDRLASE59
SUPFAMiSSF51445 SSF51445, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q5SNX7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQTHNFLCII SVILGCSAQF YVIDDRIGLG REFDGIGGLS GGGATSRLLV
60 70 80 90 100
NYEEPYRSQI LDYLFKPNFG ASLHILKVEI GGDAQTTDGT EPSHMHSKDE
110 120 130 140 150
GNFFRGYEWW LMKEAKKRNP NIKLIGLPWA FPGWVGYGTQ WPYFFPNVTA
160 170 180 190 200
NYVITWVMGA KQHHNLDIDY IGIWNEKAFD PTYIKVLRDA LDRAGFTNIG
210 220 230 240 250
IIAADGDWSI ASAMLDDPYL NDAVEVIGVH YPGTNTVKEA LLTERKLWSS
260 270 280 290 300
EDYSTYNDDI GAGCWARILN QNYVNGKMTS TISWNVIASY YENLSFGRDG
310 320 330 340 350
LMTAEEPWSG HYIVESPIWM TAHTTQFTQP GWFYLQTVGK LNHGGSYVAL
360 370 380 390 400
TDRKGNLTII IETMTHEHSQ CIRPPLPHFD VSPQIATFEL KGSFAHLADL
410 420 430 440 450
QVWYSKLDFK SGNGTLFKQL RPIRAHNGLL SLKLDVDEVF TITTVTTAQR
460 470 480 490 500
GFYPEPPKSC PFPKNYTDDF TIDNPPFSEA PYFADQTGVF EYFRNTTDNS
510 520 530 540 550
SHAFTLRQVV TERPVAWAKD ADQTISIIGD YSWSDVNVSC DVFIETPKTG
560 570 580 590 600
GVFLAARVDQ GGESVRQAKG VFYWIYANGT YRVTNDIGGK RVLAEGLSGT
610 620 630 640 650
KAGVWYTLTL SVKGYFATGS LNGFPLWKNA AVLEPKSGWA ALGTLSFEYA
660
QFDNFNVIAG
Length:660
Mass (Da):73,820
Last modified:December 21, 2004 - v1
Checksum:iAC70CBB2903A9B77
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti11S → F in AAH83517 (Ref. 2) Curated1
Sequence conflicti190A → V in AAH83517 (Ref. 2) Curated1
Sequence conflicti418K → R in AAH83517 (Ref. 2) Curated1
Sequence conflicti511T → I in AAH83517 (Ref. 2) Curated1
Sequence conflicti591R → G in AAH83517 (Ref. 2) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL953887 Genomic DNA Translation: CAI11729.1
BC083517 mRNA Translation: AAH83517.1
RefSeqiNP_001005921.1, NM_001005921.1
UniGeneiDr.106140

Genome annotation databases

EnsembliENSDART00000053870; ENSDARP00000053869; ENSDARG00000037064
GeneIDi449649
KEGGidre:449649

Similar proteinsi

Entry informationi

Entry nameiGALC_DANRE
AccessioniPrimary (citable) accession number: Q5SNX7
Secondary accession number(s): Q5XJ01
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: December 21, 2004
Last modified: May 23, 2018
This is version 82 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Cookie policy

We would like to use anonymized google analytics cookies to gather statistics on how uniprot.org is used in aggregate. Learn more

UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health