Q5SNX7 (GALC_DANRE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Galactocerebrosidase
Short name=GALCERase
EC=3.2.1.46

Alternative name(s):
Galactosylceramidase

Gene names

Name:	galc
Synonyms:	galca
ORF Names:	si:ch211-199l3.4, zgc:92561

Organism

Danio rerio (Zebrafish) (Brachydanio rerio)

Taxonomic identifier

7955 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Ostariophysi › Cypriniformes › Cyprinidae › Danio

Protein attributes

Sequence length	660 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Hydrolyzes the galactose ester bonds of galactosylceramide, galactosylsphingosine, lactosylceramide, and monogalactosyldiglyceride By similarity.
Catalytic activity	D-galactosyl-N-acylsphingosine + H2O = D-galactose + N-acylsphingosine.
Subcellular location	Lysosome By similarity.
Sequence similarities	Belongs to the glycosyl hydrolase 59 family.

Ontologies

Keywords
Cellular component	Lysosome
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro galactosylceramide catabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	lysosome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	cation binding Inferred from electronic annotation. Source: InterPro galactosylceramidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 18	18	Potential
	Chain	19 – 660	642	Galactocerebrosidase		PRO_0000370713
Amino acid modifications
	Glycosylation	147	1	N-linked (GlcNAc...) Potential
	Glycosylation	293	1	N-linked (GlcNAc...) Potential
	Glycosylation	356	1	N-linked (GlcNAc...) Potential
	Glycosylation	413	1	N-linked (GlcNAc...) Potential
	Glycosylation	465	1	N-linked (GlcNAc...) Potential
	Glycosylation	495	1	N-linked (GlcNAc...) Potential
	Glycosylation	499	1	N-linked (GlcNAc...) Potential
	Glycosylation	537	1	N-linked (GlcNAc...) Potential
	Glycosylation	578	1	N-linked (GlcNAc...) Potential
Experimental info
	Sequence conflict	11	1	S → F in AAH83517. Ref.2
	Sequence conflict	190	1	A → V in AAH83517. Ref.2
	Sequence conflict	418	1	K → R in AAH83517. Ref.2
	Sequence conflict	511	1	T → I in AAH83517. Ref.2
	Sequence conflict	591	1	R → G in AAH83517. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q5SNX7 [UniParc]. Last modified December 21, 2004. Version 1. Checksum: AC70CBB2903A9B77	FASTA	660	73,820
	10 20 30 40 50 60 MQTHNFLCII SVILGCSAQF YVIDDRIGLG REFDGIGGLS GGGATSRLLV NYEEPYRSQI 70 80 90 100 110 120 LDYLFKPNFG ASLHILKVEI GGDAQTTDGT EPSHMHSKDE GNFFRGYEWW LMKEAKKRNP 130 140 150 160 170 180 NIKLIGLPWA FPGWVGYGTQ WPYFFPNVTA NYVITWVMGA KQHHNLDIDY IGIWNEKAFD 190 200 210 220 230 240 PTYIKVLRDA LDRAGFTNIG IIAADGDWSI ASAMLDDPYL NDAVEVIGVH YPGTNTVKEA 250 260 270 280 290 300 LLTERKLWSS EDYSTYNDDI GAGCWARILN QNYVNGKMTS TISWNVIASY YENLSFGRDG 310 320 330 340 350 360 LMTAEEPWSG HYIVESPIWM TAHTTQFTQP GWFYLQTVGK LNHGGSYVAL TDRKGNLTII 370 380 390 400 410 420 IETMTHEHSQ CIRPPLPHFD VSPQIATFEL KGSFAHLADL QVWYSKLDFK SGNGTLFKQL 430 440 450 460 470 480 RPIRAHNGLL SLKLDVDEVF TITTVTTAQR GFYPEPPKSC PFPKNYTDDF TIDNPPFSEA 490 500 510 520 530 540 PYFADQTGVF EYFRNTTDNS SHAFTLRQVV TERPVAWAKD ADQTISIIGD YSWSDVNVSC 550 560 570 580 590 600 DVFIETPKTG GVFLAARVDQ GGESVRQAKG VFYWIYANGT YRVTNDIGGK RVLAEGLSGT 610 620 630 640 650 660 KAGVWYTLTL SVKGYFATGS LNGFPLWKNA AVLEPKSGWA ALGTLSFEYA QFDNFNVIAG

References

[1]	The Danio rerio sequencing project at the Sanger Institute Submitted (JAN-2009) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Tuebingen.
[2]	NIH - Zebrafish Gene Collection (ZGC) project Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: AB.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL953887 Genomic DNA. Translation: CAI11729.1. BC083517 mRNA. Translation: AAH83517.1.
IPI	IPI00483273.
RefSeq	NP_001005921.1. NM_001005921.1.
UniGene	Dr.106140.
3D structure databases
ProteinModelPortal	Q5SNX7.
ModBase	Search...
Protein-protein interaction databases
STRING	Q5SNX7.
Protein family/group databases
CAZy	GH59. Glycoside Hydrolase Family 59.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSDART00000053870; ENSDARP00000053869; ENSDARG00000037064.
GeneID	449649.
KEGG	dre:449649.
Organism-specific databases
CTD	449649.
ZFIN	ZDB-GENE-040724-243. galca.
Phylogenomic databases
eggNOG	fiNOG04988.
GeneTree	ENSGT00390000003303.
HOGENOM	HBG357031.
InParanoid	Q5SNX7.
OMA	DGTESSH.
OrthoDB	EOG4XSKPG.
PhylomeDB	Q5SNX7.
Gene expression databases
Bgee	Q5SNX7.
Family and domain databases
InterPro	IPR001286. Glyco_hydro_59. IPR013781. Glyco_hydro_subgr_catalytic. IPR017853. Glycoside_hydrolase_SF. [Graphical view]
Gene3D	G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
KO	K01202.
PANTHER	PTHR15172. Glyco_hydro_59. 1 hit.
Pfam	PF02057. Glyco_hydro_59. 1 hit. [Graphical view]
PRINTS	PR00850. GLHYDRLASE59.
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GALC_DANRE

Accession

Primary (citable) accession number: Q5SNX7
Secondary accession number(s): Q5XJ01

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 5, 2009
Last sequence update:	December 21, 2004
Last modified:	November 16, 2011
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program