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Protein

Mitogen-activated protein kinase 8

Gene

MPK8

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulationi

Activated by threonine and tyrosine phosphorylation.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei42 – 421ATPPROSITE-ProRule annotation
Active sitei139 – 1391Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi19 – 279ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. MAP kinase activity Source: UniProtKB-EC
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Mitogen-activated protein kinase 8 (EC:2.7.11.24)
Short name:
MAP kinase 8
Alternative name(s):
OsWJUMK1
Wound- and JA-uninducible MAP kinase 1
Gene namesi
Name:MPK8
Synonyms:WJUMK1
Ordered Locus Names:Os01g0665200, LOC_Os01g47530
ORF Names:P0003E08.19-1
OrganismiOryza sativa subsp. japonica (Rice)
Taxonomic identifieri39947 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza
ProteomesiUP000000763 Componenti: Chromosome 1

Organism-specific databases

GrameneiQ5SN53.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 569569Mitogen-activated protein kinase 8PRO_0000239751Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei175 – 1751PhosphothreonineBy similarity
Modified residuei177 – 1771PhosphotyrosineBy similarity

Post-translational modificationi

Dually phosphorylated on Thr-175 and Tyr-177, which activates the enzyme.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiQ5SN53.

Expressioni

Tissue specificityi

Expressed in leaves and panicles.1 Publication

Inductioni

By heavy metals, abscisic acid (ABA) and infection with rice blast fungus (M.grisea). Down-regulated by high temperature and UV-C.2 Publications

Gene expression databases

ExpressionAtlasiQ5SN53. baseline.

Structurei

3D structure databases

ProteinModelPortaliQ5SN53.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini13 – 304292Protein kinasePROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi175 – 1773TXY

Domaini

The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0515.
HOGENOMiHOG000233024.
InParanoidiQ5SN53.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS01351. MAPK. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5SN53-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDFFSEYGDA NRYKIQEVIG KGSYGVVCSA IDQHTGDKVA IKKIHNIFEH
60 70 80 90 100
LSDAARILRE IKLLRLLRHP DIVEIKHIML PPSRRDFKDI YVVFELMDTD
110 120 130 140 150
LHQVIKANDD LTKEHHQFFL YQMLRALKYI HTANVYHRDL KPKNILANAN
160 170 180 190 200
CKLKICDFGL ARVAFNDTPT TVFWTDYVAT RWYRAPELCG SFFTKYSPAI
210 220 230 240 250
DIWSIGCIFA EILTGKPLFP GKNVVHQLDL MTDLLGTPSM DTVTRIRNEK
260 270 280 290 300
ARRYLSSMRK KQPVPFSERF PKADPAALKL LQRLLAFDPK DRPTAEEALA
310 320 330 340 350
DPYFKGLAKA EREPSCQPIT KMEFEFERRK VTKEDVKELI FREILEYHPQ
360 370 380 390 400
LLKDYMNGTE KTNFLYPSAL DNFRRQFANL EENGGKNGDA VPSDRKHVSL
410 420 430 440 450
PRTTTVHSAP IPPKDHQNIT SQVPQRIPGR TGRGACPVIP FENLSAMGPY
460 470 480 490 500
NQRRVVRNPV LPPATTNLSA YAYHRKSDSS ERELQQELEK DRMRYQPSEH
510 520 530 540 550
FMDAKVVSHM SHDLRASSYY VSKAKSDVAD RAALQSNMMQ GIGPFNGIAA
560
VGGNYNKVST VQYGVSRMY
Length:569
Mass (Da):65,179
Last modified:June 13, 2006 - v2
Checksum:iA5B701306B92AC47
GO

Sequence cautioni

The sequence BAD72352.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ512643 mRNA. Translation: CAD54742.1.
AP003222 Genomic DNA. Translation: BAD72351.1.
AP003222 Genomic DNA. Translation: BAD72352.1. Sequence problems.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ512643 mRNA. Translation: CAD54742.1.
AP003222 Genomic DNA. Translation: BAD72351.1.
AP003222 Genomic DNA. Translation: BAD72352.1. Sequence problems.

3D structure databases

ProteinModelPortaliQ5SN53.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiQ5SN53.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Organism-specific databases

GrameneiQ5SN53.

Phylogenomic databases

eggNOGiCOG0515.
HOGENOMiHOG000233024.
InParanoidiQ5SN53.

Gene expression databases

ExpressionAtlasiQ5SN53. baseline.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS01351. MAPK. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Novel rice MAP kinases OsMSRMK3 and OsWJUMK1 involved in encountering diverse environmental stresses and developmental regulation."
    Agrawal G.K., Agrawal S.K., Shibato J., Iwahashi H., Rakwal R.
    Biochem. Biophys. Res. Commun. 300:775-783(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, INDUCTION.
    Strain: cv. Nipponbare.
    Tissue: Leaf.
  2. "The genome sequence and structure of rice chromosome 1."
    Sasaki T., Matsumoto T., Yamamoto K., Sakata K., Baba T., Katayose Y., Wu J., Niimura Y., Cheng Z., Nagamura Y., Antonio B.A., Kanamori H., Hosokawa S., Masukawa M., Arikawa K., Chiden Y., Hayashi M., Okamoto M.
    , Ando T., Aoki H., Arita K., Hamada M., Harada C., Hijishita S., Honda M., Ichikawa Y., Idonuma A., Iijima M., Ikeda M., Ikeno M., Ito S., Ito T., Ito Y., Ito Y., Iwabuchi A., Kamiya K., Karasawa W., Katagiri S., Kikuta A., Kobayashi N., Kono I., Machita K., Maehara T., Mizuno H., Mizubayashi T., Mukai Y., Nagasaki H., Nakashima M., Nakama Y., Nakamichi Y., Nakamura M., Namiki N., Negishi M., Ohta I., Ono N., Saji S., Sakai K., Shibata M., Shimokawa T., Shomura A., Song J., Takazaki Y., Terasawa K., Tsuji K., Waki K., Yamagata H., Yamane H., Yoshiki S., Yoshihara R., Yukawa K., Zhong H., Iwama H., Endo T., Ito H., Hahn J.H., Kim H.-I., Eun M.-Y., Yano M., Jiang J., Gojobori T.
    Nature 420:312-316(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Nipponbare.
  3. "Molecular analysis of the rice MAP kinase gene family in relation to Magnaporthe grisea infection."
    Reyna N.S., Yang Y.
    Mol. Plant Microbe Interact. 19:530-540(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION, NOMENCLATURE.

Entry informationi

Entry nameiMPK8_ORYSJ
AccessioniPrimary (citable) accession number: Q5SN53
Secondary accession number(s): Q8H0P0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 13, 2006
Last sequence update: June 13, 2006
Last modified: January 7, 2015
This is version 60 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Oryza sativa (rice)
    Index of Oryza sativa entries and their corresponding gene designations
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.