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Q5SN53 (MPK8_ORYSJ) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 57. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Mitogen-activated protein kinase 8

Short name=MAP kinase 8
EC=2.7.11.24
Alternative name(s):
OsWJUMK1
Wound- and JA-uninducible MAP kinase 1
Gene names
Name:MPK8
Synonyms:WJUMK1
Ordered Locus Names:Os01g0665200, LOC_Os01g47530
ORF Names:P0003E08.19-1
OrganismOryza sativa subsp. japonica (Rice) [Reference proteome]
Taxonomic identifier39947 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza

Protein attributes

Sequence length569 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulation

Activated by threonine and tyrosine phosphorylation By similarity.

Tissue specificity

Expressed in leaves and panicles. Ref.1

Induction

By heavy metals, abscisic acid (ABA) and infection with rice blast fungus (M.grisea). Down-regulated by high temperature and UV-C. Ref.1 Ref.3

Domain

The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

Post-translational modification

Dually phosphorylated on Thr-175 and Tyr-177, which activates the enzyme By similarity.

Sequence similarities

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.

Contains 1 protein kinase domain.

Sequence caution

The sequence BAD72352.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 569569Mitogen-activated protein kinase 8
PRO_0000239751

Regions

Domain13 – 304292Protein kinase
Nucleotide binding19 – 279ATP By similarity
Motif175 – 1773TXY

Sites

Active site1391Proton acceptor By similarity
Binding site421ATP By similarity

Amino acid modifications

Modified residue1751Phosphothreonine By similarity
Modified residue1771Phosphotyrosine By similarity

Sequences

Sequence LengthMass (Da)Tools
Q5SN53 [UniParc].

Last modified June 13, 2006. Version 2.
Checksum: A5B701306B92AC47

FASTA56965,179
        10         20         30         40         50         60 
MDFFSEYGDA NRYKIQEVIG KGSYGVVCSA IDQHTGDKVA IKKIHNIFEH LSDAARILRE 

        70         80         90        100        110        120 
IKLLRLLRHP DIVEIKHIML PPSRRDFKDI YVVFELMDTD LHQVIKANDD LTKEHHQFFL 

       130        140        150        160        170        180 
YQMLRALKYI HTANVYHRDL KPKNILANAN CKLKICDFGL ARVAFNDTPT TVFWTDYVAT 

       190        200        210        220        230        240 
RWYRAPELCG SFFTKYSPAI DIWSIGCIFA EILTGKPLFP GKNVVHQLDL MTDLLGTPSM 

       250        260        270        280        290        300 
DTVTRIRNEK ARRYLSSMRK KQPVPFSERF PKADPAALKL LQRLLAFDPK DRPTAEEALA 

       310        320        330        340        350        360 
DPYFKGLAKA EREPSCQPIT KMEFEFERRK VTKEDVKELI FREILEYHPQ LLKDYMNGTE 

       370        380        390        400        410        420 
KTNFLYPSAL DNFRRQFANL EENGGKNGDA VPSDRKHVSL PRTTTVHSAP IPPKDHQNIT 

       430        440        450        460        470        480 
SQVPQRIPGR TGRGACPVIP FENLSAMGPY NQRRVVRNPV LPPATTNLSA YAYHRKSDSS 

       490        500        510        520        530        540 
ERELQQELEK DRMRYQPSEH FMDAKVVSHM SHDLRASSYY VSKAKSDVAD RAALQSNMMQ 

       550        560 
GIGPFNGIAA VGGNYNKVST VQYGVSRMY 

« Hide

References

« Hide 'large scale' references
[1]"Novel rice MAP kinases OsMSRMK3 and OsWJUMK1 involved in encountering diverse environmental stresses and developmental regulation."
Agrawal G.K., Agrawal S.K., Shibato J., Iwahashi H., Rakwal R.
Biochem. Biophys. Res. Commun. 300:775-783(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, INDUCTION.
Strain: cv. Nipponbare.
Tissue: Leaf.
[2]"The genome sequence and structure of rice chromosome 1."
Sasaki T., Matsumoto T., Yamamoto K., Sakata K., Baba T., Katayose Y., Wu J., Niimura Y., Cheng Z., Nagamura Y., Antonio B.A., Kanamori H., Hosokawa S., Masukawa M., Arikawa K., Chiden Y., Hayashi M., Okamoto M. expand/collapse author list , Ando T., Aoki H., Arita K., Hamada M., Harada C., Hijishita S., Honda M., Ichikawa Y., Idonuma A., Iijima M., Ikeda M., Ikeno M., Ito S., Ito T., Ito Y., Ito Y., Iwabuchi A., Kamiya K., Karasawa W., Katagiri S., Kikuta A., Kobayashi N., Kono I., Machita K., Maehara T., Mizuno H., Mizubayashi T., Mukai Y., Nagasaki H., Nakashima M., Nakama Y., Nakamichi Y., Nakamura M., Namiki N., Negishi M., Ohta I., Ono N., Saji S., Sakai K., Shibata M., Shimokawa T., Shomura A., Song J., Takazaki Y., Terasawa K., Tsuji K., Waki K., Yamagata H., Yamane H., Yoshiki S., Yoshihara R., Yukawa K., Zhong H., Iwama H., Endo T., Ito H., Hahn J.H., Kim H.-I., Eun M.-Y., Yano M., Jiang J., Gojobori T.
Nature 420:312-316(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Nipponbare.
[3]"Molecular analysis of the rice MAP kinase gene family in relation to Magnaporthe grisea infection."
Reyna N.S., Yang Y.
Mol. Plant Microbe Interact. 19:530-540(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION, NOMENCLATURE.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AJ512643 mRNA. Translation: CAD54742.1.
AP003222 Genomic DNA. Translation: BAD72351.1.
AP003222 Genomic DNA. Translation: BAD72352.1. Sequence problems.

3D structure databases

ProteinModelPortalQ5SN53.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PRIDEQ5SN53.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Organism-specific databases

GrameneQ5SN53.

Phylogenomic databases

eggNOGCOG0515.
HOGENOMHOG000233024.

Family and domain databases

InterProIPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
[Graphical view]
PfamPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMSSF56112. SSF56112. 1 hit.
PROSITEPS01351. MAPK. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMPK8_ORYSJ
AccessionPrimary (citable) accession number: Q5SN53
Secondary accession number(s): Q8H0P0
Entry history
Integrated into UniProtKB/Swiss-Prot: June 13, 2006
Last sequence update: June 13, 2006
Last modified: April 16, 2014
This is version 57 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Oryza sativa (rice)

Index of Oryza sativa entries and their corresponding gene designations