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Q5SN53

- MPK8_ORYSJ

UniProt

Q5SN53 - MPK8_ORYSJ

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Protein
Mitogen-activated protein kinase 8
Gene
MPK8, WJUMK1, Os01g0665200, LOC_Os01g47530, P0003E08.19-1
Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulationi

Activated by threonine and tyrosine phosphorylation By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei42 – 421ATP By similarity
Active sitei139 – 1391Proton acceptor By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi19 – 279ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. MAP kinase activity Source: UniProtKB-EC
Complete GO annotation...

GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Mitogen-activated protein kinase 8 (EC:2.7.11.24)
    Short name:
    MAP kinase 8
    Alternative name(s):
    OsWJUMK1
    Wound- and JA-uninducible MAP kinase 1
    Gene namesi
    Name:MPK8
    Synonyms:WJUMK1
    Ordered Locus Names:Os01g0665200, LOC_Os01g47530
    ORF Names:P0003E08.19-1
    OrganismiOryza sativa subsp. japonica (Rice)
    Taxonomic identifieri39947 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza
    ProteomesiUP000000763: Chromosome 1

    Organism-specific databases

    GrameneiQ5SN53.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 569569Mitogen-activated protein kinase 8
    PRO_0000239751Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei175 – 1751Phosphothreonine By similarity
    Modified residuei177 – 1771Phosphotyrosine By similarity

    Post-translational modificationi

    Dually phosphorylated on Thr-175 and Tyr-177, which activates the enzyme By similarity.

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PRIDEiQ5SN53.

    Expressioni

    Tissue specificityi

    Expressed in leaves and panicles.1 Publication

    Inductioni

    By heavy metals, abscisic acid (ABA) and infection with rice blast fungus (M.grisea). Down-regulated by high temperature and UV-C.2 Publications

    Structurei

    3D structure databases

    ProteinModelPortaliQ5SN53.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini13 – 304292Protein kinase
    Add
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi175 – 1773TXY

    Domaini

    The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0515.
    HOGENOMiHOG000233024.

    Family and domain databases

    InterProiIPR011009. Kinase-like_dom.
    IPR003527. MAP_kinase_CS.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    [Graphical view]
    PfamiPF00069. Pkinase. 1 hit.
    [Graphical view]
    SMARTiSM00220. S_TKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 1 hit.
    PROSITEiPS01351. MAPK. 1 hit.
    PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q5SN53-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDFFSEYGDA NRYKIQEVIG KGSYGVVCSA IDQHTGDKVA IKKIHNIFEH    50
    LSDAARILRE IKLLRLLRHP DIVEIKHIML PPSRRDFKDI YVVFELMDTD 100
    LHQVIKANDD LTKEHHQFFL YQMLRALKYI HTANVYHRDL KPKNILANAN 150
    CKLKICDFGL ARVAFNDTPT TVFWTDYVAT RWYRAPELCG SFFTKYSPAI 200
    DIWSIGCIFA EILTGKPLFP GKNVVHQLDL MTDLLGTPSM DTVTRIRNEK 250
    ARRYLSSMRK KQPVPFSERF PKADPAALKL LQRLLAFDPK DRPTAEEALA 300
    DPYFKGLAKA EREPSCQPIT KMEFEFERRK VTKEDVKELI FREILEYHPQ 350
    LLKDYMNGTE KTNFLYPSAL DNFRRQFANL EENGGKNGDA VPSDRKHVSL 400
    PRTTTVHSAP IPPKDHQNIT SQVPQRIPGR TGRGACPVIP FENLSAMGPY 450
    NQRRVVRNPV LPPATTNLSA YAYHRKSDSS ERELQQELEK DRMRYQPSEH 500
    FMDAKVVSHM SHDLRASSYY VSKAKSDVAD RAALQSNMMQ GIGPFNGIAA 550
    VGGNYNKVST VQYGVSRMY 569
    Length:569
    Mass (Da):65,179
    Last modified:June 13, 2006 - v2
    Checksum:iA5B701306B92AC47
    GO

    Sequence cautioni

    The sequence BAD72352.1 differs from that shown. Reason: Erroneous gene model prediction.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ512643 mRNA. Translation: CAD54742.1.
    AP003222 Genomic DNA. Translation: BAD72351.1.
    AP003222 Genomic DNA. Translation: BAD72352.1. Sequence problems.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ512643 mRNA. Translation: CAD54742.1 .
    AP003222 Genomic DNA. Translation: BAD72351.1 .
    AP003222 Genomic DNA. Translation: BAD72352.1 . Sequence problems.

    3D structure databases

    ProteinModelPortali Q5SN53.
    ModBasei Search...

    Proteomic databases

    PRIDEi Q5SN53.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Organism-specific databases

    Gramenei Q5SN53.

    Phylogenomic databases

    eggNOGi COG0515.
    HOGENOMi HOG000233024.

    Family and domain databases

    InterProi IPR011009. Kinase-like_dom.
    IPR003527. MAP_kinase_CS.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    [Graphical view ]
    Pfami PF00069. Pkinase. 1 hit.
    [Graphical view ]
    SMARTi SM00220. S_TKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56112. SSF56112. 1 hit.
    PROSITEi PS01351. MAPK. 1 hit.
    PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    « Hide 'large scale' publications
    1. "Novel rice MAP kinases OsMSRMK3 and OsWJUMK1 involved in encountering diverse environmental stresses and developmental regulation."
      Agrawal G.K., Agrawal S.K., Shibato J., Iwahashi H., Rakwal R.
      Biochem. Biophys. Res. Commun. 300:775-783(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, INDUCTION.
      Strain: cv. Nipponbare.
      Tissue: Leaf.
    2. "The genome sequence and structure of rice chromosome 1."
      Sasaki T., Matsumoto T., Yamamoto K., Sakata K., Baba T., Katayose Y., Wu J., Niimura Y., Cheng Z., Nagamura Y., Antonio B.A., Kanamori H., Hosokawa S., Masukawa M., Arikawa K., Chiden Y., Hayashi M., Okamoto M.
      , Ando T., Aoki H., Arita K., Hamada M., Harada C., Hijishita S., Honda M., Ichikawa Y., Idonuma A., Iijima M., Ikeda M., Ikeno M., Ito S., Ito T., Ito Y., Ito Y., Iwabuchi A., Kamiya K., Karasawa W., Katagiri S., Kikuta A., Kobayashi N., Kono I., Machita K., Maehara T., Mizuno H., Mizubayashi T., Mukai Y., Nagasaki H., Nakashima M., Nakama Y., Nakamichi Y., Nakamura M., Namiki N., Negishi M., Ohta I., Ono N., Saji S., Sakai K., Shibata M., Shimokawa T., Shomura A., Song J., Takazaki Y., Terasawa K., Tsuji K., Waki K., Yamagata H., Yamane H., Yoshiki S., Yoshihara R., Yukawa K., Zhong H., Iwama H., Endo T., Ito H., Hahn J.H., Kim H.-I., Eun M.-Y., Yano M., Jiang J., Gojobori T.
      Nature 420:312-316(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Nipponbare.
    3. "Molecular analysis of the rice MAP kinase gene family in relation to Magnaporthe grisea infection."
      Reyna N.S., Yang Y.
      Mol. Plant Microbe Interact. 19:530-540(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION, NOMENCLATURE.

    Entry informationi

    Entry nameiMPK8_ORYSJ
    AccessioniPrimary (citable) accession number: Q5SN53
    Secondary accession number(s): Q8H0P0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 13, 2006
    Last sequence update: June 13, 2006
    Last modified: April 16, 2014
    This is version 57 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Oryza sativa (rice)
      Index of Oryza sativa entries and their corresponding gene designations
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3

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