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Q5SN53

- MPK8_ORYSJ

UniProt

Q5SN53 - MPK8_ORYSJ

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Protein

Mitogen-activated protein kinase 8

Gene
MPK8, WJUMK1, Os01g0665200, LOC_Os01g47530, P0003E08.19-1
Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulationi

Activated by threonine and tyrosine phosphorylation By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei42 – 421ATP By similarity
Active sitei139 – 1391Proton acceptor By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi19 – 279ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. MAP kinase activity Source: UniProtKB-EC
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Mitogen-activated protein kinase 8 (EC:2.7.11.24)
Short name:
MAP kinase 8
Alternative name(s):
OsWJUMK1
Wound- and JA-uninducible MAP kinase 1
Gene namesi
Name:MPK8
Synonyms:WJUMK1
Ordered Locus Names:Os01g0665200, LOC_Os01g47530
ORF Names:P0003E08.19-1
OrganismiOryza sativa subsp. japonica (Rice)
Taxonomic identifieri39947 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza
ProteomesiUP000000763: Chromosome 1

Organism-specific databases

GrameneiQ5SN53.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 569569Mitogen-activated protein kinase 8PRO_0000239751Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei175 – 1751Phosphothreonine By similarity
Modified residuei177 – 1771Phosphotyrosine By similarity

Post-translational modificationi

Dually phosphorylated on Thr-175 and Tyr-177, which activates the enzyme By similarity.

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiQ5SN53.

Expressioni

Tissue specificityi

Expressed in leaves and panicles.1 Publication

Inductioni

By heavy metals, abscisic acid (ABA) and infection with rice blast fungus (M.grisea). Down-regulated by high temperature and UV-C.2 Publications

Structurei

3D structure databases

ProteinModelPortaliQ5SN53.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini13 – 304292Protein kinaseAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi175 – 1773TXY

Domaini

The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0515.
HOGENOMiHOG000233024.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS01351. MAPK. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5SN53-1 [UniParc]FASTAAdd to Basket

« Hide

MDFFSEYGDA NRYKIQEVIG KGSYGVVCSA IDQHTGDKVA IKKIHNIFEH    50
LSDAARILRE IKLLRLLRHP DIVEIKHIML PPSRRDFKDI YVVFELMDTD 100
LHQVIKANDD LTKEHHQFFL YQMLRALKYI HTANVYHRDL KPKNILANAN 150
CKLKICDFGL ARVAFNDTPT TVFWTDYVAT RWYRAPELCG SFFTKYSPAI 200
DIWSIGCIFA EILTGKPLFP GKNVVHQLDL MTDLLGTPSM DTVTRIRNEK 250
ARRYLSSMRK KQPVPFSERF PKADPAALKL LQRLLAFDPK DRPTAEEALA 300
DPYFKGLAKA EREPSCQPIT KMEFEFERRK VTKEDVKELI FREILEYHPQ 350
LLKDYMNGTE KTNFLYPSAL DNFRRQFANL EENGGKNGDA VPSDRKHVSL 400
PRTTTVHSAP IPPKDHQNIT SQVPQRIPGR TGRGACPVIP FENLSAMGPY 450
NQRRVVRNPV LPPATTNLSA YAYHRKSDSS ERELQQELEK DRMRYQPSEH 500
FMDAKVVSHM SHDLRASSYY VSKAKSDVAD RAALQSNMMQ GIGPFNGIAA 550
VGGNYNKVST VQYGVSRMY 569
Length:569
Mass (Da):65,179
Last modified:June 13, 2006 - v2
Checksum:iA5B701306B92AC47
GO

Sequence cautioni

The sequence BAD72352.1 differs from that shown. Reason: Erroneous gene model prediction.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ512643 mRNA. Translation: CAD54742.1.
AP003222 Genomic DNA. Translation: BAD72351.1.
AP003222 Genomic DNA. Translation: BAD72352.1. Sequence problems.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ512643 mRNA. Translation: CAD54742.1 .
AP003222 Genomic DNA. Translation: BAD72351.1 .
AP003222 Genomic DNA. Translation: BAD72352.1 . Sequence problems.

3D structure databases

ProteinModelPortali Q5SN53.
ModBasei Search...
MobiDBi Search...

Proteomic databases

PRIDEi Q5SN53.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Organism-specific databases

Gramenei Q5SN53.

Phylogenomic databases

eggNOGi COG0515.
HOGENOMi HOG000233024.

Family and domain databases

InterProi IPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
[Graphical view ]
Pfami PF00069. Pkinase. 1 hit.
[Graphical view ]
SMARTi SM00220. S_TKc. 1 hit.
[Graphical view ]
SUPFAMi SSF56112. SSF56112. 1 hit.
PROSITEi PS01351. MAPK. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Novel rice MAP kinases OsMSRMK3 and OsWJUMK1 involved in encountering diverse environmental stresses and developmental regulation."
    Agrawal G.K., Agrawal S.K., Shibato J., Iwahashi H., Rakwal R.
    Biochem. Biophys. Res. Commun. 300:775-783(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, INDUCTION.
    Strain: cv. Nipponbare.
    Tissue: Leaf.
  2. "The genome sequence and structure of rice chromosome 1."
    Sasaki T., Matsumoto T., Yamamoto K., Sakata K., Baba T., Katayose Y., Wu J., Niimura Y., Cheng Z., Nagamura Y., Antonio B.A., Kanamori H., Hosokawa S., Masukawa M., Arikawa K., Chiden Y., Hayashi M., Okamoto M.
    , Ando T., Aoki H., Arita K., Hamada M., Harada C., Hijishita S., Honda M., Ichikawa Y., Idonuma A., Iijima M., Ikeda M., Ikeno M., Ito S., Ito T., Ito Y., Ito Y., Iwabuchi A., Kamiya K., Karasawa W., Katagiri S., Kikuta A., Kobayashi N., Kono I., Machita K., Maehara T., Mizuno H., Mizubayashi T., Mukai Y., Nagasaki H., Nakashima M., Nakama Y., Nakamichi Y., Nakamura M., Namiki N., Negishi M., Ohta I., Ono N., Saji S., Sakai K., Shibata M., Shimokawa T., Shomura A., Song J., Takazaki Y., Terasawa K., Tsuji K., Waki K., Yamagata H., Yamane H., Yoshiki S., Yoshihara R., Yukawa K., Zhong H., Iwama H., Endo T., Ito H., Hahn J.H., Kim H.-I., Eun M.-Y., Yano M., Jiang J., Gojobori T.
    Nature 420:312-316(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Nipponbare.
  3. "Molecular analysis of the rice MAP kinase gene family in relation to Magnaporthe grisea infection."
    Reyna N.S., Yang Y.
    Mol. Plant Microbe Interact. 19:530-540(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION, NOMENCLATURE.

Entry informationi

Entry nameiMPK8_ORYSJ
AccessioniPrimary (citable) accession number: Q5SN53
Secondary accession number(s): Q8H0P0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 13, 2006
Last sequence update: June 13, 2006
Last modified: April 16, 2014
This is version 57 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Oryza sativa (rice)
    Index of Oryza sativa entries and their corresponding gene designations
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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