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Protein

Phosphoribosylformylglycinamidine synthase subunit PurL

Gene

purL

Organism
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Part of the phosphoribosylformylglycinamidine synthase complex involved in the purines biosynthetic pathway. Catalyzes the ATP-dependent conversion of formylglycinamide ribonucleotide (FGAR) and glutamine to yield formylglycinamidine ribonucleotide (FGAM) and glutamate. The FGAM synthase complex is composed of three subunits. PurQ produces an ammonia molecule by converting glutamine to glutamate. PurL transfers the ammonia molecule to FGAR to form FGAM in an ATP-dependent manner. PurS interacts with PurQ and PurL and is thought to assist in the transfer of the ammonia molecule from PurQ to PurL.UniRule annotation

Catalytic activityi

ATP + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O = ADP + phosphate + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine + L-glutamate.UniRule annotation

Pathwayi: IMP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Phosphoribosylformylglycinamidine synthase subunit PurL (purL), Phosphoribosylformylglycinamidine synthase subunit PurQ (purQ), Phosphoribosylformylglycinamidine synthase subunit PurS (purS)
  2. Phosphoribosylformylglycinamidine cyclo-ligase (purM)
This subpathway is part of the pathway IMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide, the pathway IMP biosynthesis via de novo pathway and in Purine metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei41 – 411UniRule annotation
Active sitei87 – 871Proton acceptorUniRule annotation
Binding sitei108 – 1081SubstrateUniRule annotation
Binding sitei109 – 1091ATP
Binding sitei211 – 2111Substrate; via carbonyl oxygenUniRule annotation
Binding sitei231 – 2311SubstrateUniRule annotation
Metal bindingi430 – 4301Magnesium1 Publication
Metal bindingi472 – 4721Magnesium1 Publication
Binding sitei485 – 4851ATP
Binding sitei525 – 5251SubstrateCurated
Metal bindingi620 – 6201Magnesium1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi66 – 672ATP
Nucleotide bindingi83 – 853ATP
Nucleotide bindingi522 – 5232ATP

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Purine biosynthesis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciTTHE300852:GH8R-1556-MONOMER.
UniPathwayiUPA00074; UER00128.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoribosylformylglycinamidine synthase subunit PurLUniRule annotation (EC:6.3.5.3UniRule annotation)
Short name:
FGAM synthaseUniRule annotation
Alternative name(s):
Formylglycinamide ribonucleotide amidotransferase subunit IIUniRule annotation
Short name:
FGAR amidotransferase IIUniRule annotation
Short name:
FGAR-AT IIUniRule annotation
Glutamine amidotransferase PurLUniRule annotation
Phosphoribosylformylglycinamidine synthase subunit IIUniRule annotation
Gene namesi
Name:purLUniRule annotation
Ordered Locus Names:TTHA1519
OrganismiThermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Taxonomic identifieri300852 [NCBI]
Taxonomic lineageiBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus
Proteomesi
  • UP000000532 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 725725Phosphoribosylformylglycinamidine synthase subunit PurLPRO_0000100503Add
BLAST

Interactioni

Subunit structurei

Monomer. Part of the FGAM synthase complex composed of 1 PurL, 1 PurQ and 2 PurS subunits.UniRule annotation

Protein-protein interaction databases

STRINGi300852.TTHA1519.

Structurei

Secondary structure

1
725
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi2 – 76Combined sources
Helixi12 – 2211Combined sources
Helixi28 – 3710Combined sources
Helixi40 – 434Combined sources
Turni45 – 473Combined sources
Helixi48 – 514Combined sources
Beta strandi61 – 633Combined sources
Beta strandi67 – 759Combined sources
Beta strandi78 – 869Combined sources
Helixi88 – 936Combined sources
Helixi95 – 11218Combined sources
Beta strandi116 – 12712Combined sources
Helixi132 – 15221Combined sources
Beta strandi156 – 1638Combined sources
Helixi165 – 1673Combined sources
Beta strandi168 – 1703Combined sources
Beta strandi172 – 18211Combined sources
Helixi183 – 1853Combined sources
Beta strandi196 – 2027Combined sources
Helixi235 – 25016Combined sources
Beta strandi254 – 2596Combined sources
Helixi264 – 27512Combined sources
Beta strandi278 – 2836Combined sources
Helixi284 – 2863Combined sources
Beta strandi289 – 2935Combined sources
Helixi296 – 3016Combined sources
Beta strandi305 – 3128Combined sources
Helixi317 – 32610Combined sources
Beta strandi331 – 34717Combined sources
Beta strandi350 – 3567Combined sources
Helixi357 – 3615Combined sources
Helixi374 – 3807Combined sources
Helixi391 – 3999Combined sources
Turni402 – 4043Combined sources
Helixi408 – 4114Combined sources
Beta strandi420 – 4245Combined sources
Beta strandi430 – 4367Combined sources
Beta strandi439 – 44810Combined sources
Helixi451 – 4566Combined sources
Helixi458 – 47518Combined sources
Beta strandi479 – 48810Combined sources
Beta strandi492 – 4943Combined sources
Helixi495 – 51521Combined sources
Beta strandi519 – 5257Combined sources
Beta strandi538 – 54811Combined sources
Beta strandi554 – 5563Combined sources
Beta strandi563 – 5686Combined sources
Helixi578 – 5847Combined sources
Helixi596 – 61116Combined sources
Beta strandi616 – 6205Combined sources
Helixi625 – 63410Combined sources
Turni635 – 6373Combined sources
Beta strandi641 – 6444Combined sources
Helixi650 – 6534Combined sources
Beta strandi661 – 6655Combined sources
Helixi667 – 6693Combined sources
Helixi670 – 67910Combined sources
Beta strandi684 – 6907Combined sources
Beta strandi692 – 6998Combined sources
Beta strandi702 – 7076Combined sources
Helixi708 – 72316Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3VIUX-ray2.35A1-725[»]
ProteinModelPortaliQ5SMH8.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ5SMH8.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni86 – 894Substrate bindingCurated
Regioni303 – 3053Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the FGAMS family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4108JIU. Bacteria.
COG0046. LUCA.
HOGENOMiHOG000238227.
KOiK01952.
OMAiVMWQFAE.
PhylomeDBiQ5SMH8.

Family and domain databases

Gene3Di3.30.1330.10. 2 hits.
HAMAPiMF_00420. PurL_2. 1 hit.
InterProiIPR010918. AIR_synth_C_dom.
IPR010074. PRibForGlyAmidine_synth_PurL.
IPR016188. PurM-like_N.
[Graphical view]
PfamiPF00586. AIRS. 2 hits.
PF02769. AIRS_C. 2 hits.
[Graphical view]
PIRSFiPIRSF001587. FGAM_synthase_II. 1 hit.
SUPFAMiSSF56042. SSF56042. 2 hits.
TIGRFAMsiTIGR01736. FGAM_synth_II. 1 hit.

Sequencei

Sequence statusi: Complete.

Q5SMH8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEALAKEIGI PEGEYREIVQ RLGREPNRVE LLLFKVMWSE HCAYKNSRPL
60 70 80 90 100
LKALPKEGEA VLQGPGENAG VVRVGEGWAV AFKIESHNHP SAVEPFQGAA
110 120 130 140 150
TGVGGILRDI MSMGARPIAL LDSLRFGPPE EARSRYLLKG VVSGIAFYGN
160 170 180 190 200
AIGVPTVGGD LYFHEGYREN PLVNAMCLGL LREEHLKRSR ASLGRPIYYA
210 220 230 240 250
GAKTGRDGIG GAAFASRELK EEKAEDRPAV QVGDPFLGKL LMEATLEAIE
260 270 280 290 300
LDLVEGVQDM GAAGLTSSLS ELAHKSGLGV ELHLDLVPTR EEGMTPEELL
310 320 330 340 350
LSESQERMVL VPKEGKEKAL EEVFGRWGLD CVPVARTIPE RVFRVLFRGE
360 370 380 390 400
VVAEVPTEAL AEAPTYVRVG REDPEVRRLR ETPIPPLEAD PQEVLRRLLA
410 420 430 440 450
SPNLASREAV YERYDHQVGT RTALLPGKGD AAVLWIKGTR LGVAAKVDQN
460 470 480 490 500
PRYSRLHPRL GAMHALAEAC RNVSVVGAKP LAYTDGLNLG SPETPEGYHE
510 520 530 540 550
LAETIAGLKE ASEALGVPVV SGNVSLYNES GGKRIPPTAM VGVVGVLEVD
560 570 580 590 600
KRAEMGFRRP GEVLLLIGEE RGELGASEVL YLLTGKEFGH PPRLDLGREK
610 620 630 640 650
AVQEAIRDLI QRGLTRTAHD VAEGGLLLAL AEMTFPYGVG ATVEVREEGL
660 670 680 690 700
EALFGEAPSR VLFTVEKTRL QEATLLLEER GLPYRVLGET GGKSLTVLTP
710 720
GGVLEWSLEE LLSAWKAPLR EVLDG
Length:725
Mass (Da):78,643
Last modified:December 21, 2004 - v1
Checksum:iC5CC5FAE833F0199
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP008226 Genomic DNA. Translation: BAD71342.1.
RefSeqiWP_011173566.1. NC_006461.1.
YP_144785.1. NC_006461.1.

Genome annotation databases

EnsemblBacteriaiBAD71342; BAD71342; BAD71342.
GeneIDi3168862.
KEGGittj:TTHA1519.
PATRICi23957999. VBITheThe93045_1494.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP008226 Genomic DNA. Translation: BAD71342.1.
RefSeqiWP_011173566.1. NC_006461.1.
YP_144785.1. NC_006461.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3VIUX-ray2.35A1-725[»]
ProteinModelPortaliQ5SMH8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi300852.TTHA1519.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAD71342; BAD71342; BAD71342.
GeneIDi3168862.
KEGGittj:TTHA1519.
PATRICi23957999. VBITheThe93045_1494.

Phylogenomic databases

eggNOGiENOG4108JIU. Bacteria.
COG0046. LUCA.
HOGENOMiHOG000238227.
KOiK01952.
OMAiVMWQFAE.
PhylomeDBiQ5SMH8.

Enzyme and pathway databases

UniPathwayiUPA00074; UER00128.
BioCyciTTHE300852:GH8R-1556-MONOMER.

Miscellaneous databases

EvolutionaryTraceiQ5SMH8.

Family and domain databases

Gene3Di3.30.1330.10. 2 hits.
HAMAPiMF_00420. PurL_2. 1 hit.
InterProiIPR010918. AIR_synth_C_dom.
IPR010074. PRibForGlyAmidine_synth_PurL.
IPR016188. PurM-like_N.
[Graphical view]
PfamiPF00586. AIRS. 2 hits.
PF02769. AIRS_C. 2 hits.
[Graphical view]
PIRSFiPIRSF001587. FGAM_synthase_II. 1 hit.
SUPFAMiSSF56042. SSF56042. 2 hits.
TIGRFAMsiTIGR01736. FGAM_synth_II. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiPURL_THET8
AccessioniPrimary (citable) accession number: Q5SMH8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: December 21, 2004
Last modified: September 7, 2016
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.