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Protein

Magnesium transporter MgtE

Gene

TTHA1060

Organism
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acts as a highly selective magnesium channel.2 Publications

Enzyme regulationi

The channel activity is regulated by the intracellular magnesium concentration. Under high-intracellular magnesium conditions, binding of magnesium to the cytosolic domains stabilizes the closed conformation of the channel. Under low-intracellular magnesium conditions, the channel is in equilibrium between the open and closed states.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi91Magnesium 11
Metal bindingi95Magnesium 21
Metal bindingi136Magnesium 2; via carbonyl oxygen1
Metal bindingi214Magnesium 31
Metal bindingi216Magnesium 41
Metal bindingi223Magnesium 5; via carbonyl oxygen1
Metal bindingi226Magnesium 51
Metal bindingi247Magnesium 11
Metal bindingi255Magnesium 31
Metal bindingi258Magnesium 31
Metal bindingi259Magnesium 41
Metal bindingi418Magnesium 41
Metal bindingi428Magnesium 6; via carbonyl oxygen1
Metal bindingi432Magnesium 61

GO - Molecular functioni

  • magnesium ion binding Source: UniProtKB
  • magnesium ion transmembrane transporter activity Source: InterPro
  • protein homodimerization activity Source: UniProtKB

GO - Biological processi

  • magnesium ion transport Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Transport

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciTTHE300852:GH8R-1058-MONOMER.
BRENDAi3.6.3.2. 2305.

Protein family/group databases

TCDBi1.A.26.1.2. the mg(2+) transporter-e (mgte) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Magnesium transporter MgtE
Gene namesi
Ordered Locus Names:TTHA1060
OrganismiThermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Taxonomic identifieri300852 [NCBI]
Taxonomic lineageiBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus
Proteomesi
  • UP000000532 Componenti: Chromosome

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 277CytoplasmicAdd BLAST277
Transmembranei278 – 305Helical; Name=1Add BLAST28
Intramembranei306 – 320Add BLAST15
Transmembranei321 – 344Helical; Name=2Add BLAST24
Topological domaini345 – 351Cytoplasmic7
Transmembranei352 – 381Helical; Name=3Add BLAST30
Intramembranei382 – 3854
Transmembranei386 – 414Helical; Name=4Add BLAST29
Intramembranei415 – 42410
Transmembranei425 – 447Helical; Name=5Add BLAST23
Topological domaini448 – 450Periplasmic3

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi59E → A: Still possesses a slight channel activity. 1 Publication1
Mutagenesisi226D → N: Abolishes the Mg(2+)-dependent suppression of the Mg(2+) influx; when associated with A-250. 1 Publication1
Mutagenesisi250D → A: Abolishes the Mg(2+)-dependent suppression of the Mg(2+) influx; when associated with N-226. 1 Publication1
Mutagenesisi258E → Q: Abolishes the Mg(2+)-dependent suppression of the Mg(2+) influx. 1 Publication1
Mutagenesisi259D → N: Abolishes the Mg(2+)-dependent suppression of the Mg(2+) influx. 1 Publication1
Mutagenesisi285R → A: Abolishes Mg(2+)-transport activity. 1 Publication1
Mutagenesisi318F → A: Abolishes Mg(2+)-transport activity. 1 Publication1
Mutagenesisi321P → A: Abolishes Mg(2+)-transport activity. 1 Publication1
Mutagenesisi324L → A: Abolishes Mg(2+)-transport activity. 1 Publication1
Mutagenesisi329N → A: Abolishes Mg(2+)-transport activity. 1 Publication1
Mutagenesisi332N → A: Does not affect activity. 1 Publication1
Mutagenesisi333Q → A: Abolishes Mg(2+)-transport activity. 1 Publication1
Mutagenesisi359E → A: Abolishes Mg(2+)-transport activity. 1 Publication1
Mutagenesisi424N → A: Does not affect activity. 1 Publication1
Mutagenesisi432D → A or N: Abolishes Mg(2+)-transport activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003638891 – 450Magnesium transporter MgtEAdd BLAST450

Interactioni

Subunit structurei

Homodimer.1 Publication

GO - Molecular functioni

  • protein homodimerization activity Source: UniProtKB

Protein-protein interaction databases

DIPiDIP-60248N.
STRINGi300852.TTHA1060.

Structurei

Secondary structure

1450
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni7 – 9Combined sources3
Helixi10 – 15Combined sources6
Helixi18 – 27Combined sources10
Helixi30 – 35Combined sources6
Helixi36 – 39Combined sources4
Helixi42 – 51Combined sources10
Helixi54 – 62Combined sources9
Helixi66 – 75Combined sources10
Helixi78 – 87Combined sources10
Helixi90 – 103Combined sources14
Helixi105 – 114Combined sources10
Helixi117 – 128Combined sources12
Helixi134 – 136Combined sources3
Beta strandi142 – 145Combined sources4
Helixi151 – 161Combined sources11
Turni162 – 164Combined sources3
Beta strandi168 – 174Combined sources7
Beta strandi179 – 185Combined sources7
Helixi186 – 191Combined sources6
Turni199 – 201Combined sources3
Beta strandi202 – 205Combined sources4
Beta strandi209 – 214Combined sources6
Helixi215 – 225Combined sources11
Beta strandi228 – 233Combined sources6
Beta strandi237 – 244Combined sources8
Helixi245 – 251Combined sources7
Beta strandi263 – 265Combined sources3
Turni273 – 275Combined sources3
Helixi278 – 296Combined sources19
Helixi298 – 304Combined sources7
Helixi307 – 312Combined sources6
Helixi314 – 319Combined sources6
Helixi320 – 343Combined sources24
Helixi349 – 351Combined sources3
Helixi352 – 381Combined sources30
Helixi384 – 386Combined sources3
Helixi387 – 414Combined sources28
Helixi419 – 421Combined sources3
Helixi423 – 447Combined sources25

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2YVXX-ray3.50A/B/C/D1-450[»]
2YVYX-ray2.30A1-275[»]
2YVZX-ray3.90A/B1-275[»]
2ZY9X-ray2.94A/B1-450[»]
4U9LX-ray2.30A/B271-449[»]
4U9NX-ray2.20A/B271-448[»]
4WIBX-ray3.20A/B271-448[»]
ProteinModelPortaliQ5SMG8.
SMRiQ5SMG8.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ5SMG8.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini138 – 200CBS 1PROSITE-ProRule annotationAdd BLAST63
Domaini202 – 258CBS 2PROSITE-ProRule annotationAdd BLAST57

Sequence similaritiesi

Belongs to the SLC41A transporter family.Curated
Contains 2 CBS domains.PROSITE-ProRule annotation

Keywords - Domaini

CBS domain, Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105CJW. Bacteria.
COG2239. LUCA.
HOGENOMiHOG000280152.
KOiK06213.
OMAiLKLSDWW.
PhylomeDBiQ5SMG8.

Family and domain databases

Gene3Di1.10.357.20. 1 hit.
InterProiIPR000644. CBS_dom.
IPR006668. Mg_transptr_MgtE_intracell_dom.
IPR006669. MgtE_transporter.
IPR006667. SLC41_membr_dom.
[Graphical view]
PfamiPF00571. CBS. 2 hits.
PF01769. MgtE. 1 hit.
PF03448. MgtE_N. 1 hit.
[Graphical view]
SMARTiSM00116. CBS. 2 hits.
SM00924. MgtE_N. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00400. mgtE. 1 hit.
PROSITEiPS51371. CBS. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5SMG8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEEKLAVSLQ EALQEGDTRA LREVLEEIHP QDLLALWDEL KGEHRYVVLT
60 70 80 90 100
LLPKAKAAEV LSHLSPEEQA EYLKTLPPWR LREILEELSL DDLADALQAV
110 120 130 140 150
RKEDPAYFQR LKDLLDPRTR AEVEALARYE EDEAGGLMTP EYVAVREGMT
160 170 180 190 200
VEEVLRFLRR AAPDAETIYY IYVVDEKGRL KGVLSLRDLI VADPRTRVAE
210 220 230 240 250
IMNPKVVYVR TDTDQEEVAR LMADYDFTVL PVVDEEGRLV GIVTVDDVLD
260 270 280 290 300
VLEAEATEDI HKLGAVDVPD LVYSEAGPVA LWLARVRWLV ILILTGMVTS
310 320 330 340 350
SILQGFESVL EAVTALAFYV PVLLGTGGNT GNQSATLIIR ALATRDLDLR
360 370 380 390 400
DWRRVFLKEM GVGLLLGLTL SFLLVGKVYW DGHPLLLPVV GVSLVLIVFF
410 420 430 440 450
ANLVGAMLPF LLRRLGVDPA LVSNPLVATL SDVTGLLIYL SVARLLLEAV
Length:450
Mass (Da):50,078
Last modified:December 21, 2004 - v1
Checksum:i6F5930DCDDB90B05
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP008226 Genomic DNA. Translation: BAD70883.1.
RefSeqiWP_011228410.1. NC_006461.1.
YP_144326.1. NC_006461.1.

Genome annotation databases

EnsemblBacteriaiBAD70883; BAD70883; BAD70883.
GeneIDi3168925.
KEGGittj:TTHA1060.
PATRICi23957080. VBITheThe93045_1040.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP008226 Genomic DNA. Translation: BAD70883.1.
RefSeqiWP_011228410.1. NC_006461.1.
YP_144326.1. NC_006461.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2YVXX-ray3.50A/B/C/D1-450[»]
2YVYX-ray2.30A1-275[»]
2YVZX-ray3.90A/B1-275[»]
2ZY9X-ray2.94A/B1-450[»]
4U9LX-ray2.30A/B271-449[»]
4U9NX-ray2.20A/B271-448[»]
4WIBX-ray3.20A/B271-448[»]
ProteinModelPortaliQ5SMG8.
SMRiQ5SMG8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-60248N.
STRINGi300852.TTHA1060.

Protein family/group databases

TCDBi1.A.26.1.2. the mg(2+) transporter-e (mgte) family.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAD70883; BAD70883; BAD70883.
GeneIDi3168925.
KEGGittj:TTHA1060.
PATRICi23957080. VBITheThe93045_1040.

Phylogenomic databases

eggNOGiENOG4105CJW. Bacteria.
COG2239. LUCA.
HOGENOMiHOG000280152.
KOiK06213.
OMAiLKLSDWW.
PhylomeDBiQ5SMG8.

Enzyme and pathway databases

BioCyciTTHE300852:GH8R-1058-MONOMER.
BRENDAi3.6.3.2. 2305.

Miscellaneous databases

EvolutionaryTraceiQ5SMG8.

Family and domain databases

Gene3Di1.10.357.20. 1 hit.
InterProiIPR000644. CBS_dom.
IPR006668. Mg_transptr_MgtE_intracell_dom.
IPR006669. MgtE_transporter.
IPR006667. SLC41_membr_dom.
[Graphical view]
PfamiPF00571. CBS. 2 hits.
PF01769. MgtE. 1 hit.
PF03448. MgtE_N. 1 hit.
[Graphical view]
SMARTiSM00116. CBS. 2 hits.
SM00924. MgtE_N. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00400. mgtE. 1 hit.
PROSITEiPS51371. CBS. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMGTE_THET8
AccessioniPrimary (citable) accession number: Q5SMG8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 10, 2009
Last sequence update: December 21, 2004
Last modified: November 2, 2016
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.