UniProtKB - Q5SM60 (RSMA_THET8)
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Protein
Ribosomal RNA small subunit methyltransferase A
Gene
rsmA
Organism
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Status
Functioni
Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits.UniRule annotation
Catalytic activityi
4 S-adenosyl-L-methionine + adenine(1518)/adenine(1519) in 16S rRNA = 4 S-adenosyl-L-homocysteine + N6-dimethyladenine(1518)/N6-dimethyladenine(1519) in 16S rRNA.UniRule annotation
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 28 | S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation | 1 | |
Binding sitei | 30 | S-adenosyl-L-methionine; via amide nitrogenUniRule annotation | 1 | |
Binding sitei | 54 | S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation | 1 | |
Binding sitei | 75 | S-adenosyl-L-methionine | 1 | |
Binding sitei | 99 | S-adenosyl-L-methionine | 1 | |
Binding sitei | 117 | S-adenosyl-L-methionine | 1 |
GO - Molecular functioni
- 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase activity Source: UniProtKB-EC
- RNA binding Source: UniProtKB-KW
- rRNA (adenine-N6,N6-)-dimethyltransferase activity Source: InterPro
Keywordsi
Molecular function | Methyltransferase, RNA-binding, Transferase |
Biological process | rRNA processing |
Ligand | S-adenosyl-L-methionine |
Enzyme and pathway databases
BioCyci | TTHE300852:G1GKC-86-MONOMER. |
BRENDAi | 2.1.1.182. 2305. |
Names & Taxonomyi
Protein namesi | Recommended name: Ribosomal RNA small subunit methyltransferase AUniRule annotation (EC:2.1.1.182UniRule annotation)Alternative name(s): 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferaseUniRule annotation 16S rRNA dimethyladenosine transferaseUniRule annotation 16S rRNA dimethylaseUniRule annotation S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferaseUniRule annotation |
Gene namesi | Name:rsmAUniRule annotation Synonyms:ksgAUniRule annotation Ordered Locus Names:TTHA0083 |
Organismi | Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) |
Taxonomic identifieri | 300852 [NCBI] |
Taxonomic lineagei | Bacteria › Deinococcus-Thermus › Deinococci › Thermales › Thermaceae › Thermus › |
Proteomesi |
|
Subcellular locationi
- Cytoplasm UniRule annotation
GO - Cellular componenti
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000101631 | 1 – 271 | Ribosomal RNA small subunit methyltransferase AAdd BLAST | 271 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more detailsFeature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Helixi | 7 – 16 | Combined sources | 10 | |
Helixi | 23 – 25 | Combined sources | 3 | |
Helixi | 33 – 43 | Combined sources | 11 | |
Beta strandi | 50 – 53 | Combined sources | 4 | |
Turni | 56 – 58 | Combined sources | 3 | |
Helixi | 59 – 66 | Combined sources | 8 | |
Beta strandi | 71 – 76 | Combined sources | 6 | |
Helixi | 78 – 80 | Combined sources | 3 | |
Helixi | 81 – 87 | Combined sources | 7 | |
Turni | 88 – 90 | Combined sources | 3 | |
Beta strandi | 94 – 98 | Combined sources | 5 | |
Helixi | 100 – 102 | Combined sources | 3 | |
Helixi | 105 – 107 | Combined sources | 3 | |
Beta strandi | 112 – 118 | Combined sources | 7 | |
Turni | 120 – 122 | Combined sources | 3 | |
Helixi | 124 – 133 | Combined sources | 10 | |
Beta strandi | 136 – 144 | Combined sources | 9 | |
Helixi | 145 – 151 | Combined sources | 7 | |
Helixi | 162 – 170 | Combined sources | 9 | |
Beta strandi | 171 – 179 | Combined sources | 9 | |
Helixi | 181 – 183 | Combined sources | 3 | |
Beta strandi | 184 – 186 | Combined sources | 3 | |
Beta strandi | 192 – 199 | Combined sources | 8 | |
Helixi | 206 – 215 | Combined sources | 10 | |
Helixi | 223 – 229 | Combined sources | 7 | |
Helixi | 234 – 243 | Combined sources | 10 | |
Helixi | 252 – 254 | Combined sources | 3 | |
Helixi | 257 – 267 | Combined sources | 11 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3FUT | X-ray | 1.52 | A/B | 1-271 | [»] | |
3FUU | X-ray | 1.53 | A | 1-271 | [»] | |
3FUV | X-ray | 1.95 | A/B/C | 1-271 | [»] | |
3FUW | X-ray | 1.56 | A | 1-271 | [»] | |
3FUX | X-ray | 1.68 | A/B/C | 1-271 | [»] | |
ProteinModelPortali | Q5SM60. | |||||
SMRi | Q5SM60. | |||||
ModBasei | Search... | |||||
MobiDBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q5SM60. |
Family & Domainsi
Sequence similaritiesi
Belongs to the class I-like SAM-binding methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily.UniRule annotation
Phylogenomic databases
eggNOGi | ENOG4105D1X. Bacteria. COG0030. LUCA. |
HOGENOMi | HOG000227961. |
KOi | K02528. |
OMAi | KRFGQHW. |
PhylomeDBi | Q5SM60. |
Family and domain databases
Gene3Di | 1.10.8.100. 1 hit. |
HAMAPi | MF_00607. 16SrRNA_methyltr_A. 1 hit. |
InterProi | View protein in InterPro IPR001737. KsgA/Erm. IPR023165. rRNA_Ade_diMease-like. IPR020596. rRNA_Ade_Mease_Trfase_CS. IPR020598. rRNA_Ade_methylase_Trfase_N. IPR011530. rRNA_adenine_dimethylase. IPR029063. SAM-dependent_MTases. |
PANTHERi | PTHR11727. PTHR11727. 1 hit. |
Pfami | View protein in Pfam PF00398. RrnaAD. 1 hit. |
SMARTi | View protein in SMART SM00650. rADc. 1 hit. |
SUPFAMi | SSF53335. SSF53335. 1 hit. |
TIGRFAMsi | TIGR00755. ksgA. 1 hit. |
PROSITEi | View protein in PROSITE PS01131. RRNA_A_DIMETH. 1 hit. PS51689. SAM_RNA_A_N6_MT. 1 hit. |
i Sequence
Sequence statusi: Complete.
Q5SM60-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSKLASPQSV RALLERHGLF ADKRFGQNFL VSEAHLRRIV EAARPFTGPV
60 70 80 90 100
FEVGPGLGAL TRALLEAGAE VTAIEKDLRL RPVLEETLSG LPVRLVFQDA
110 120 130 140 150
LLYPWEEVPQ GSLLVANLPY HIATPLVTRL LKTGRFARLV FLVQKEVAER
160 170 180 190 200
MTARPKTPAY GVLTLRVAHH AVAERLFDLP PGAFFPPPKV WSSLVRLTPT
210 220 230 240 250
GALDDPGLFR LVEAAFGKRR KTLLNALAAA GYPKARVEEA LRALGLPPRV
260 270
RAEELDLEAF RRLREGLEGA V
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AP008226 Genomic DNA. Translation: BAD69906.1. |
RefSeqi | WP_011227698.1. NC_006461.1. YP_143349.1. NC_006461.1. |
Genome annotation databases
EnsemblBacteriai | BAD69906; BAD69906; BAD69906. |
GeneIDi | 3169411. |
KEGGi | ttj:TTHA0083. |
PATRICi | fig|300852.9.peg.81. |
Similar proteinsi
Entry informationi
Entry namei | RSMA_THET8 | |
Accessioni | Q5SM60Primary (citable) accession number: Q5SM60 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 26, 2005 |
Last sequence update: | December 21, 2004 | |
Last modified: | February 28, 2018 | |
This is version 85 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |