rsmA

Functionⁱ

Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits.UniRule annotation

Catalytic activityⁱ

4 S-adenosyl-L-methionine + adenine(1518)/adenine(1519) in 16S rRNA = 4 S-adenosyl-L-homocysteine + N₆-dimethyladenine(1518)/N₆-dimethyladenine(1519) in 16S rRNA.UniRule annotation

Sites

Feature key	Position(s)	DescriptionActions	Length
Binding siteⁱ	28	S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation	1
Binding siteⁱ	30	S-adenosyl-L-methionine; via amide nitrogenUniRule annotation	1
Binding siteⁱ	54	S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation	1
Binding siteⁱ	75	S-adenosyl-L-methionine	1
Binding siteⁱ	99	S-adenosyl-L-methionine	1
Binding siteⁱ	117	S-adenosyl-L-methionine	1

GO - Molecular functionⁱ

16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase activity Source: UniProtKB-EC
RNA binding Source: UniProtKB-KW
rRNA (adenine-N6,N6-)-dimethyltransferase activity Source: InterPro

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Methyltransferase, RNA-binding, Transferase
Biological process	rRNA processing
Ligand	S-adenosyl-L-methionine

Enzyme and pathway databases

BioCycⁱ	TTHE300852:G1GKC-86-MONOMER.
BRENDAⁱ	2.1.1.182. 2305.

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Ribosomal RNA small subunit methyltransferase AUniRule annotation (EC:2.1.1.182UniRule annotation) Alternative name(s): 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferaseUniRule annotation 16S rRNA dimethyladenosine transferaseUniRule annotation 16S rRNA dimethylaseUniRule annotation S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferaseUniRule annotation
Gene namesⁱ	Name:rsmAUniRule annotation Synonyms:ksgAUniRule annotation Ordered Locus Names:TTHA0083
Organismⁱ	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Taxonomic identifierⁱ	300852 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Bacteria › Terrabacteria group › Deinococcus-Thermus › Deinococci › Thermales › Thermaceae › Thermus › Thermus thermophilus
Proteomesⁱ	UP000000532 Componentⁱ: Chromosome

Subcellular locationⁱ

Cytoplasm
UniRule annotation
Manual assertion according to rulesⁱ
- HAMAP-Rule:MF_00607

GO - Cellular componentⁱ

cytoplasm Source: UniProtKB-SubCell

View the complete GO annotation on QuickGO ...

Keywords - Cellular componentⁱ

Cytoplasm

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000101631	1 – 271	Ribosomal RNA small subunit methyltransferase AAdd BLAST		271

Interactionⁱ

Protein-protein interaction databases

STRINGⁱ	300852.TTHA0083.

STRINGⁱ

300852.TTHA0083.

Structureⁱ

Secondary structure

Legend: HelixTurnBeta strandPDB Structure known for this area

Show more details

Feature key	Position(s)	DescriptionActions	Length
Helixⁱ	7 – 16	Combined sources	10
Helixⁱ	23 – 25	Combined sources	3
Helixⁱ	33 – 43	Combined sources	11
Beta strandⁱ	50 – 53	Combined sources	4
Turnⁱ	56 – 58	Combined sources	3
Helixⁱ	59 – 66	Combined sources	8
Beta strandⁱ	71 – 76	Combined sources	6
Helixⁱ	78 – 80	Combined sources	3
Helixⁱ	81 – 87	Combined sources	7
Turnⁱ	88 – 90	Combined sources	3
Beta strandⁱ	94 – 98	Combined sources	5
Helixⁱ	100 – 102	Combined sources	3
Helixⁱ	105 – 107	Combined sources	3
Beta strandⁱ	112 – 118	Combined sources	7
Turnⁱ	120 – 122	Combined sources	3
Helixⁱ	124 – 133	Combined sources	10
Beta strandⁱ	136 – 144	Combined sources	9
Helixⁱ	145 – 151	Combined sources	7
Helixⁱ	162 – 170	Combined sources	9
Beta strandⁱ	171 – 179	Combined sources	9
Helixⁱ	181 – 183	Combined sources	3
Beta strandⁱ	184 – 186	Combined sources	3
Beta strandⁱ	192 – 199	Combined sources	8
Helixⁱ	206 – 215	Combined sources	10
Helixⁱ	223 – 229	Combined sources	7
Helixⁱ	234 – 243	Combined sources	10
Helixⁱ	252 – 254	Combined sources	3
Helixⁱ	257 – 267	Combined sources	11

3D structure databases

Select the link destinations: Protein Data Bank Europe More...PDBeⁱ Protein Data Bank RCSB More...RCSB PDBⁱ Protein Data Bank Japan More...PDBjⁱ Links Updated	PDB entry	Method	Resolution (Å)	Chain	Positions	PDBsum
	3FUT	X-ray	1.52	A/B	1-271	[»]
	3FUU	X-ray	1.53	A	1-271	[»]
	3FUV	X-ray	1.95	A/B/C	1-271	[»]
	3FUW	X-ray	1.56	A	1-271	[»]
	3FUX	X-ray	1.68	A/B/C	1-271	[»]
ProteinModelPortalⁱ	Q5SM60.
SMRⁱ	Q5SM60.
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Miscellaneous databases

EvolutionaryTraceⁱ	Q5SM60.

EvolutionaryTraceⁱ

Q5SM60.

Family & Domainsⁱ

Sequence similaritiesⁱ

Belongs to the class I-like SAM-binding methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily.UniRule annotation

Phylogenomic databases

eggNOGⁱ	ENOG4105D1X. Bacteria. COG0030. LUCA.
HOGENOMⁱ	HOG000227961.
KEGG Orthology (KO) More...KOⁱ	K02528.
OMAⁱ	KRFGQHW.
PhylomeDBⁱ	Q5SM60.

Family and domain databases

Gene3Dⁱ	1.10.8.100. 1 hit.
HAMAPⁱ	MF_00607. 16SrRNA_methyltr_A. 1 hit.
InterProⁱ	View protein in InterPro IPR001737. KsgA/Erm. IPR023165. rRNA_Ade_diMease-like. IPR020596. rRNA_Ade_Mease_Trfase_CS. IPR020598. rRNA_Ade_methylase_Trfase_N. IPR011530. rRNA_adenine_dimethylase. IPR029063. SAM-dependent_MTases.
PANTHERⁱ	PTHR11727. PTHR11727. 1 hit.
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00398. RrnaAD. 1 hit.
SMARTⁱ	View protein in SMART SM00650. rADc. 1 hit.
SUPFAMⁱ	SSF53335. SSF53335. 1 hit.
TIGRFAMsⁱ	TIGR00755. ksgA. 1 hit.
PROSITEⁱ	View protein in PROSITE PS01131. RRNA_A_DIMETH. 1 hit. PS51689. SAM_RNA_A_N6_MT. 1 hit.

Sequenceⁱ

Sequence statusⁱ: Complete.

Q5SM60-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MSKLASPQSV RALLERHGLF ADKRFGQNFL VSEAHLRRIV EAARPFTGPV 
        60         70         80         90        100
FEVGPGLGAL TRALLEAGAE VTAIEKDLRL RPVLEETLSG LPVRLVFQDA 
       110        120        130        140        150
LLYPWEEVPQ GSLLVANLPY HIATPLVTRL LKTGRFARLV FLVQKEVAER 
       160        170        180        190        200
MTARPKTPAY GVLTLRVAHH AVAERLFDLP PGAFFPPPKV WSSLVRLTPT 
       210        220        230        240        250
GALDDPGLFR LVEAAFGKRR KTLLNALAAA GYPKARVEEA LRALGLPPRV 
       260        270 
RAEELDLEAF RRLREGLEGA V

Length:271

Mass (Da):29,866

Last modified:December 21, 2004 - v1

Checksum:ⁱ26B9BDCCAC8767B5

GO

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AP008226 Genomic DNA. Translation: BAD69906.1.
NCBI Reference Sequences More...RefSeqⁱ	WP_011227698.1. NC_006461.1. YP_143349.1. NC_006461.1.

Genome annotation databases

EnsemblBacteriaⁱ	BAD69906; BAD69906; BAD69906.
GeneIDⁱ	3169411.
KEGGⁱ	ttj:TTHA0083.
PATRICⁱ	fig\|300852.9.peg.81.

Protein	Similar proteins	Organisms	Length	Cluster ID	Cluster name	Size
Q5SM60	A0A1G7EM79 Q72GC7 A0A1J1ESR1 UPI0003A6401F	Thermus arciformis Thermus thermophilus (strain HB27 / ATCC BAA-163 / DSM 7039) Thermus thermophilus	271	UniRef90_Q5SM60	Cluster: Ribosomal RNA small subunit methyltransferase A	5

Protein	Similar proteins	Organisms	Length	Cluster ID	Cluster name	Size
Q5SM60	A0A1G7EM79 UPI0003A6401F Q72GC7 A0A1J1ESR1 UPI00037C88D2 G8NBB5 UPI000374DBFE UPI00056DA3CB F2NQ90 UPI00056F2466 +28	Thermus arciformis Thermus thermophilus Thermus thermophilus (strain HB27 / ATCC BAA-163 / DSM 7039) Meiothermus timidus Thermus sp. CCB_US3_UF1 Thermus igniterrae Thermus tengchongensis Marinithermus hydrothermalis (strain DSM 14884 / JCM 11576 / T1) Thermus caliditerrae Thermus aquaticus Y51MC23 And more	271	UniRef50_Q5SM60	Cluster: Ribosomal RNA small subunit methyltransferase A	39

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AP008226 Genomic DNA. Translation: BAD69906.1.
NCBI Reference Sequences More...RefSeqⁱ	WP_011227698.1. NC_006461.1. YP_143349.1. NC_006461.1.

3D structure databases

Select the link destinations: Protein Data Bank Europe More...PDBeⁱ Protein Data Bank RCSB More...RCSB PDBⁱ Protein Data Bank Japan More...PDBjⁱ Links Updated	PDB entry	Method	Resolution (Å)	Chain	Positions	PDBsum
	3FUT	X-ray	1.52	A/B	1-271	[»]
	3FUU	X-ray	1.53	A	1-271	[»]
	3FUV	X-ray	1.95	A/B/C	1-271	[»]
	3FUW	X-ray	1.56	A	1-271	[»]
	3FUX	X-ray	1.68	A/B/C	1-271	[»]
ProteinModelPortalⁱ	Q5SM60.
SMRⁱ	Q5SM60.
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

STRINGⁱ	300852.TTHA0083.

STRINGⁱ

300852.TTHA0083.

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

EnsemblBacteriaⁱ	BAD69906; BAD69906; BAD69906.
GeneIDⁱ	3169411.
KEGGⁱ	ttj:TTHA0083.
PATRICⁱ	fig\|300852.9.peg.81.

Phylogenomic databases

eggNOGⁱ	ENOG4105D1X. Bacteria. COG0030. LUCA.
HOGENOMⁱ	HOG000227961.
KEGG Orthology (KO) More...KOⁱ	K02528.
OMAⁱ	KRFGQHW.
PhylomeDBⁱ	Q5SM60.

Enzyme and pathway databases

BioCycⁱ	TTHE300852:G1GKC-86-MONOMER.
BRENDAⁱ	2.1.1.182. 2305.

Miscellaneous databases

EvolutionaryTraceⁱ	Q5SM60.

EvolutionaryTraceⁱ

Q5SM60.

Family and domain databases

Gene3Dⁱ	1.10.8.100. 1 hit.
HAMAPⁱ	MF_00607. 16SrRNA_methyltr_A. 1 hit.
InterProⁱ	View protein in InterPro IPR001737. KsgA/Erm. IPR023165. rRNA_Ade_diMease-like. IPR020596. rRNA_Ade_Mease_Trfase_CS. IPR020598. rRNA_Ade_methylase_Trfase_N. IPR011530. rRNA_adenine_dimethylase. IPR029063. SAM-dependent_MTases.
PANTHERⁱ	PTHR11727. PTHR11727. 1 hit.
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00398. RrnaAD. 1 hit.
SMARTⁱ	View protein in SMART SM00650. rADc. 1 hit.
SUPFAMⁱ	SSF53335. SSF53335. 1 hit.
TIGRFAMsⁱ	TIGR00755. ksgA. 1 hit.
PROSITEⁱ	View protein in PROSITE PS01131. RRNA_A_DIMETH. 1 hit. PS51689. SAM_RNA_A_N6_MT. 1 hit.
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	RSMA_THET8
Accessionⁱ	Q5SM60Primary (citable) accession number: Q5SM60
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	April 26, 2005
	Last sequence update:	December 21, 2004
	Last modified:	February 28, 2018
	This is version 85 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Prokaryotic Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

3D-structure, Complete proteome, Reference proteome

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - Q5SM60 (RSMA_THET8)

Your basket is currently empty. i <p>When browsing through different UniProt proteins, you can use the ‘basket’ to save them, so that you can back to find or analyse them later.<p><a href='/help/basket' target='_top'>More...</a></p>

Ribosomal RNA small subunit methyltransferase A

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Sites

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Cellular componenti

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

3D structure databases

Miscellaneous databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

Protocols and materials databases

Genome annotation databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Your basket is currently empty. ⁱ

Functionⁱ

GO - Molecular functionⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

GO - Cellular componentⁱ

PTM / Processingⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ