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Protein

Ribosomal RNA small subunit methyltransferase A

Gene

rsmA

Organism
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits.UniRule annotation

Catalytic activityi

4 S-adenosyl-L-methionine + adenine(1518)/adenine(1519) in 16S rRNA = 4 S-adenosyl-L-homocysteine + N(6)-dimethyladenine(1518)/N(6)-dimethyladenine(1519) in 16S rRNA.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei28 – 281S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation
Binding sitei30 – 301S-adenosyl-L-methionine; via amide nitrogenUniRule annotation
Binding sitei54 – 541S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation
Binding sitei75 – 751S-adenosyl-L-methionine
Binding sitei99 – 991S-adenosyl-L-methionine
Binding sitei117 – 1171S-adenosyl-L-methionine

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

rRNA processing

Keywords - Ligandi

RNA-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciTTHE300852:GH8R-87-MONOMER.
BRENDAi2.1.1.182. 2305.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribosomal RNA small subunit methyltransferase AUniRule annotation (EC:2.1.1.182UniRule annotation)
Alternative name(s):
16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferaseUniRule annotation
16S rRNA dimethyladenosine transferaseUniRule annotation
16S rRNA dimethylaseUniRule annotation
S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferaseUniRule annotation
Gene namesi
Name:rsmAUniRule annotation
Synonyms:ksgAUniRule annotation
Ordered Locus Names:TTHA0083
OrganismiThermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Taxonomic identifieri300852 [NCBI]
Taxonomic lineageiBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus
Proteomesi
  • UP000000532 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 271271Ribosomal RNA small subunit methyltransferase APRO_0000101631Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi300852.TTHA0083.

Structurei

Secondary structure

1
271
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi7 – 1610Combined sources
Helixi23 – 253Combined sources
Helixi33 – 4311Combined sources
Beta strandi50 – 534Combined sources
Turni56 – 583Combined sources
Helixi59 – 668Combined sources
Beta strandi71 – 766Combined sources
Helixi78 – 803Combined sources
Helixi81 – 877Combined sources
Turni88 – 903Combined sources
Beta strandi94 – 985Combined sources
Helixi100 – 1023Combined sources
Helixi105 – 1073Combined sources
Beta strandi112 – 1187Combined sources
Turni120 – 1223Combined sources
Helixi124 – 13310Combined sources
Beta strandi136 – 1449Combined sources
Helixi145 – 1517Combined sources
Helixi162 – 1709Combined sources
Beta strandi171 – 1799Combined sources
Helixi181 – 1833Combined sources
Beta strandi184 – 1863Combined sources
Beta strandi192 – 1998Combined sources
Helixi206 – 21510Combined sources
Helixi223 – 2297Combined sources
Helixi234 – 24310Combined sources
Helixi252 – 2543Combined sources
Helixi257 – 26711Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3FUTX-ray1.52A/B1-271[»]
3FUUX-ray1.53A1-271[»]
3FUVX-ray1.95A/B/C1-271[»]
3FUWX-ray1.56A1-271[»]
3FUXX-ray1.68A/B/C1-271[»]
ProteinModelPortaliQ5SM60.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ5SM60.

Family & Domainsi

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105D1X. Bacteria.
COG0030. LUCA.
HOGENOMiHOG000227961.
KOiK02528.
OMAiVKANWYA.
OrthoDBiEOG66F08Z.
PhylomeDBiQ5SM60.

Family and domain databases

Gene3Di1.10.8.100. 1 hit.
3.40.50.150. 1 hit.
HAMAPiMF_00607. 16SrRNA_methyltr_A.
InterProiIPR001737. KsgA/Erm.
IPR023165. rRNA_Ade_diMease-like.
IPR020596. rRNA_Ade_Mease_Trfase_CS.
IPR020598. rRNA_Ade_methylase_Trfase_N.
IPR011530. rRNA_adenine_dimethylase.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PANTHERiPTHR11727. PTHR11727. 1 hit.
PfamiPF00398. RrnaAD. 1 hit.
[Graphical view]
SMARTiSM00650. rADc. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00755. ksgA. 1 hit.
PROSITEiPS01131. RRNA_A_DIMETH. 1 hit.
PS51689. SAM_RNA_A_N6_MT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5SM60-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKLASPQSV RALLERHGLF ADKRFGQNFL VSEAHLRRIV EAARPFTGPV
60 70 80 90 100
FEVGPGLGAL TRALLEAGAE VTAIEKDLRL RPVLEETLSG LPVRLVFQDA
110 120 130 140 150
LLYPWEEVPQ GSLLVANLPY HIATPLVTRL LKTGRFARLV FLVQKEVAER
160 170 180 190 200
MTARPKTPAY GVLTLRVAHH AVAERLFDLP PGAFFPPPKV WSSLVRLTPT
210 220 230 240 250
GALDDPGLFR LVEAAFGKRR KTLLNALAAA GYPKARVEEA LRALGLPPRV
260 270
RAEELDLEAF RRLREGLEGA V
Length:271
Mass (Da):29,866
Last modified:December 21, 2004 - v1
Checksum:i26B9BDCCAC8767B5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP008226 Genomic DNA. Translation: BAD69906.1.
RefSeqiWP_011227698.1. NC_006461.1.
YP_143349.1. NC_006461.1.

Genome annotation databases

EnsemblBacteriaiBAD69906; BAD69906; BAD69906.
GeneIDi3169411.
KEGGittj:TTHA0083.
PATRICi23955097. VBITheThe93045_0081.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP008226 Genomic DNA. Translation: BAD69906.1.
RefSeqiWP_011227698.1. NC_006461.1.
YP_143349.1. NC_006461.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3FUTX-ray1.52A/B1-271[»]
3FUUX-ray1.53A1-271[»]
3FUVX-ray1.95A/B/C1-271[»]
3FUWX-ray1.56A1-271[»]
3FUXX-ray1.68A/B/C1-271[»]
ProteinModelPortaliQ5SM60.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi300852.TTHA0083.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAD69906; BAD69906; BAD69906.
GeneIDi3169411.
KEGGittj:TTHA0083.
PATRICi23955097. VBITheThe93045_0081.

Phylogenomic databases

eggNOGiENOG4105D1X. Bacteria.
COG0030. LUCA.
HOGENOMiHOG000227961.
KOiK02528.
OMAiVKANWYA.
OrthoDBiEOG66F08Z.
PhylomeDBiQ5SM60.

Enzyme and pathway databases

BioCyciTTHE300852:GH8R-87-MONOMER.
BRENDAi2.1.1.182. 2305.

Miscellaneous databases

EvolutionaryTraceiQ5SM60.

Family and domain databases

Gene3Di1.10.8.100. 1 hit.
3.40.50.150. 1 hit.
HAMAPiMF_00607. 16SrRNA_methyltr_A.
InterProiIPR001737. KsgA/Erm.
IPR023165. rRNA_Ade_diMease-like.
IPR020596. rRNA_Ade_Mease_Trfase_CS.
IPR020598. rRNA_Ade_methylase_Trfase_N.
IPR011530. rRNA_adenine_dimethylase.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PANTHERiPTHR11727. PTHR11727. 1 hit.
PfamiPF00398. RrnaAD. 1 hit.
[Graphical view]
SMARTiSM00650. rADc. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00755. ksgA. 1 hit.
PROSITEiPS01131. RRNA_A_DIMETH. 1 hit.
PS51689. SAM_RNA_A_N6_MT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Complete genome sequence of Thermus thermophilus HB8."
    Masui R., Kurokawa K., Nakagawa N., Tokunaga F., Koyama Y., Shibata T., Oshima T., Yokoyama S., Yasunaga T., Kuramitsu S.
    Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: HB8 / ATCC 27634 / DSM 579.
  2. "Structural rearrangements in the active site of the Thermus thermophilus 16S rRNA methyltransferase KsgA in a binary complex with 5'-methylthioadenosine."
    Demirci H., Belardinelli R., Seri E., Gregory S.T., Gualerzi C., Dahlberg A.E., Jogl G.
    J. Mol. Biol. 388:271-282(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.52 ANGSTROMS) IN COMPLEX WITH 5'-DEOXY-5'-METHYLTHIOADENOSINE.

Entry informationi

Entry nameiRSMA_THET8
AccessioniPrimary (citable) accession number: Q5SM60
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: December 21, 2004
Last modified: November 11, 2015
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.