Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Ribosomal RNA small subunit methyltransferase A

Gene

rsmA

Organism
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits.UniRule annotation

Catalytic activityi

4 S-adenosyl-L-methionine + adenine(1518)/adenine(1519) in 16S rRNA = 4 S-adenosyl-L-homocysteine + N(6)-dimethyladenine(1518)/N(6)-dimethyladenine(1519) in 16S rRNA.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei28S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation1
Binding sitei30S-adenosyl-L-methionine; via amide nitrogenUniRule annotation1
Binding sitei54S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation1
Binding sitei75S-adenosyl-L-methionine1
Binding sitei99S-adenosyl-L-methionine1
Binding sitei117S-adenosyl-L-methionine1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

rRNA processing

Keywords - Ligandi

RNA-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BRENDAi2.1.1.182. 2305.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribosomal RNA small subunit methyltransferase AUniRule annotation (EC:2.1.1.182UniRule annotation)
Alternative name(s):
16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferaseUniRule annotation
16S rRNA dimethyladenosine transferaseUniRule annotation
16S rRNA dimethylaseUniRule annotation
S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferaseUniRule annotation
Gene namesi
Name:rsmAUniRule annotation
Synonyms:ksgAUniRule annotation
Ordered Locus Names:TTHA0083
OrganismiThermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Taxonomic identifieri300852 [NCBI]
Taxonomic lineageiBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus
Proteomesi
  • UP000000532 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001016311 – 271Ribosomal RNA small subunit methyltransferase AAdd BLAST271

Interactioni

Protein-protein interaction databases

STRINGi300852.TTHA0083.

Structurei

Secondary structure

1271
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi7 – 16Combined sources10
Helixi23 – 25Combined sources3
Helixi33 – 43Combined sources11
Beta strandi50 – 53Combined sources4
Turni56 – 58Combined sources3
Helixi59 – 66Combined sources8
Beta strandi71 – 76Combined sources6
Helixi78 – 80Combined sources3
Helixi81 – 87Combined sources7
Turni88 – 90Combined sources3
Beta strandi94 – 98Combined sources5
Helixi100 – 102Combined sources3
Helixi105 – 107Combined sources3
Beta strandi112 – 118Combined sources7
Turni120 – 122Combined sources3
Helixi124 – 133Combined sources10
Beta strandi136 – 144Combined sources9
Helixi145 – 151Combined sources7
Helixi162 – 170Combined sources9
Beta strandi171 – 179Combined sources9
Helixi181 – 183Combined sources3
Beta strandi184 – 186Combined sources3
Beta strandi192 – 199Combined sources8
Helixi206 – 215Combined sources10
Helixi223 – 229Combined sources7
Helixi234 – 243Combined sources10
Helixi252 – 254Combined sources3
Helixi257 – 267Combined sources11

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3FUTX-ray1.52A/B1-271[»]
3FUUX-ray1.53A1-271[»]
3FUVX-ray1.95A/B/C1-271[»]
3FUWX-ray1.56A1-271[»]
3FUXX-ray1.68A/B/C1-271[»]
ProteinModelPortaliQ5SM60.
SMRiQ5SM60.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ5SM60.

Family & Domainsi

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105D1X. Bacteria.
COG0030. LUCA.
HOGENOMiHOG000227961.
KOiK02528.
OMAiVKANWYA.
PhylomeDBiQ5SM60.

Family and domain databases

Gene3Di1.10.8.100. 1 hit.
3.40.50.150. 1 hit.
HAMAPiMF_00607. 16SrRNA_methyltr_A. 1 hit.
InterProiIPR001737. KsgA/Erm.
IPR023165. rRNA_Ade_diMease-like.
IPR020596. rRNA_Ade_Mease_Trfase_CS.
IPR020598. rRNA_Ade_methylase_Trfase_N.
IPR011530. rRNA_adenine_dimethylase.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PANTHERiPTHR11727. PTHR11727. 1 hit.
PfamiPF00398. RrnaAD. 1 hit.
[Graphical view]
SMARTiSM00650. rADc. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00755. ksgA. 1 hit.
PROSITEiPS01131. RRNA_A_DIMETH. 1 hit.
PS51689. SAM_RNA_A_N6_MT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5SM60-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKLASPQSV RALLERHGLF ADKRFGQNFL VSEAHLRRIV EAARPFTGPV
60 70 80 90 100
FEVGPGLGAL TRALLEAGAE VTAIEKDLRL RPVLEETLSG LPVRLVFQDA
110 120 130 140 150
LLYPWEEVPQ GSLLVANLPY HIATPLVTRL LKTGRFARLV FLVQKEVAER
160 170 180 190 200
MTARPKTPAY GVLTLRVAHH AVAERLFDLP PGAFFPPPKV WSSLVRLTPT
210 220 230 240 250
GALDDPGLFR LVEAAFGKRR KTLLNALAAA GYPKARVEEA LRALGLPPRV
260 270
RAEELDLEAF RRLREGLEGA V
Length:271
Mass (Da):29,866
Last modified:December 21, 2004 - v1
Checksum:i26B9BDCCAC8767B5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP008226 Genomic DNA. Translation: BAD69906.1.
RefSeqiWP_011227698.1. NC_006461.1.
YP_143349.1. NC_006461.1.

Genome annotation databases

EnsemblBacteriaiBAD69906; BAD69906; BAD69906.
GeneIDi3169411.
KEGGittj:TTHA0083.
PATRICi23955097. VBITheThe93045_0081.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP008226 Genomic DNA. Translation: BAD69906.1.
RefSeqiWP_011227698.1. NC_006461.1.
YP_143349.1. NC_006461.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3FUTX-ray1.52A/B1-271[»]
3FUUX-ray1.53A1-271[»]
3FUVX-ray1.95A/B/C1-271[»]
3FUWX-ray1.56A1-271[»]
3FUXX-ray1.68A/B/C1-271[»]
ProteinModelPortaliQ5SM60.
SMRiQ5SM60.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi300852.TTHA0083.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAD69906; BAD69906; BAD69906.
GeneIDi3169411.
KEGGittj:TTHA0083.
PATRICi23955097. VBITheThe93045_0081.

Phylogenomic databases

eggNOGiENOG4105D1X. Bacteria.
COG0030. LUCA.
HOGENOMiHOG000227961.
KOiK02528.
OMAiVKANWYA.
PhylomeDBiQ5SM60.

Enzyme and pathway databases

BRENDAi2.1.1.182. 2305.

Miscellaneous databases

EvolutionaryTraceiQ5SM60.

Family and domain databases

Gene3Di1.10.8.100. 1 hit.
3.40.50.150. 1 hit.
HAMAPiMF_00607. 16SrRNA_methyltr_A. 1 hit.
InterProiIPR001737. KsgA/Erm.
IPR023165. rRNA_Ade_diMease-like.
IPR020596. rRNA_Ade_Mease_Trfase_CS.
IPR020598. rRNA_Ade_methylase_Trfase_N.
IPR011530. rRNA_adenine_dimethylase.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PANTHERiPTHR11727. PTHR11727. 1 hit.
PfamiPF00398. RrnaAD. 1 hit.
[Graphical view]
SMARTiSM00650. rADc. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00755. ksgA. 1 hit.
PROSITEiPS01131. RRNA_A_DIMETH. 1 hit.
PS51689. SAM_RNA_A_N6_MT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRSMA_THET8
AccessioniPrimary (citable) accession number: Q5SM60
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: December 21, 2004
Last modified: November 2, 2016
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.