Q5SM45 (SYR_THET8) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Arginine--tRNA ligase
EC=6.1.1.19

Alternative name(s):
Arginyl-tRNA synthetase
Short name=ArgRS

Gene names

Name:	argS
Ordered Locus Names:	TTHA0098

Organism

Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) [Reference proteome] [HAMAP]

Taxonomic identifier

300852 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Deinococcus-Thermus › Deinococci › Thermales › Thermaceae › Thermus › Thermus thermophilus

Protein attributes

Sequence length	592 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	ATP + L-arginine + tRNA(Arg) = AMP + diphosphate + L-arginyl-tRNA(Arg). HAMAP-Rule MF_00123
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	arginyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP arginine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 592	592	Arginine--tRNA ligase HAMAP-Rule MF_00123		PRO_0000242112
Regions
	Motif	112 – 122	11	"HIGH" region HAMAP-Rule MF_00123

Sequences

	Sequence		Length	Mass (Da)
	Q5SM45 [UniParc]. Last modified December 21, 2004. Version 1. Checksum: BA300E5F28F672E6	FASTA	592	66,227
	10 20 30 40 50 60 MLRRALEEAI AQALKEMGVP VRLKVARAPK DKPGDYGVPL FALAKELRKP PQAIAQELKD 70 80 90 100 110 120 RLPLPEFVEE AVPVGGYLNF RLRTEALLRE ALRPKAPFPR RPGVVLVEHT SVNPNKELHV 130 140 150 160 170 180 GHLRNIALGD AIARILAYAG REVLVLNYID DTGRQAAETL FALRHYGLTW DGKEKYDHFA 190 200 210 220 230 240 GRAYVRLHQD PEYERLQPAI EEVLHALERG ELREEVNRIL LAQMATMHAL NARYDLLVWE 250 260 270 280 290 300 SDIVRAGLLQ KALALLEQSP HVFRPREGKY AGALVMDASP VIPGLEDPFF VLLRSNGTAT 310 320 330 340 350 360 YYAKDIAFQF WKMGILEGLR FRPYENPYYP GLRTSAPEGE AYTPKAEETI NVVDVRQSHP 370 380 390 400 410 420 QALVRAALAL AGYPALAEKA HHLAYETVLL EGRQMSGRKG LAVSVDEVLE EATRRARAIV 430 440 450 460 470 480 EEKNPDHPDK EEAARMVALG AIRFSMVKTE PKKQIDFRYQ EALSFEGDTG PYVQYAHARA 490 500 510 520 530 540 HSILRKAGEW GAPDLSQATP YERALALDLL DFEEAVLEAA EERTPHVLAQ YLLDLAASWN 550 560 570 580 590 AYYNARENGQ PATPVLTAPE GLRELRLSLV QSLQRTLATG LDLLGIPAPE VM

References

[1]	"Complete genome sequence of Thermus thermophilus HB8." Masui R., Kurokawa K., Nakagawa N., Tokunaga F., Koyama Y., Shibata T., Oshima T., Yokoyama S., Yasunaga T., Kuramitsu S. Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: HB8 / ATCC 27634 / DSM 579.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AP008226 Genomic DNA. Translation: BAD69921.1.

RefSeq

YP_143364.1. NC_006461.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1IQ0	X-ray	2.30	A	1-592	[»]
1IR4	model	-	A	1-592	[»]

ProteinModelPortal

Q5SM45.

SMR

Q5SM45. Positions 1-592.

ModBase

Search...

Protein-protein interaction databases

STRING

300852.TTHA0098.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

BAD69921; BAD69921; BAD69921.

GeneID

3169614.

KEGG

ttj:TTHA0098.

PATRIC

23955127. VBITheThe93045_0096.

Phylogenomic databases

eggNOG

COG0018.

HOGENOM

HOG000247213.

KO

K01887.

OMA

DRGESFY.

ProtClustDB

PRK01611.

Family and domain databases

Gene3D

3.30.1360.70. 1 hit.
3.40.50.620. 1 hit.

HAMAP

MF_00123. Arg_tRNA_synth.

InterPro

IPR001412. aa-tRNA-synth_I_CS.
IPR001278. Arg-tRNA-ligase_Ia.
IPR015945. Arg-tRNA-synth_Ia_core.
IPR005148. Arg-tRNA-synth_N.
IPR008909. DALR_anticod-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
[Graphical view]

PANTHER

PTHR11956. PTHR11956. 1 hit.

Pfam

PF03485. Arg_tRNA_synt_N. 1 hit.
PF05746. DALR_1. 1 hit.
PF00750. tRNA-synt_1d. 2 hits.
[Graphical view]

PRINTS

PR01038. TRNASYNTHARG.

SMART

SM01016. Arg_tRNA_synt_N. 1 hit.
SM00836. DALR_1. 1 hit.
[Graphical view]

SUPFAM

SSF55190. Arg-tRNA-synth_Ic_N. 1 hit.
SSF47323. tRNAsyn_1a_bind. 1 hit.

PROSITE

PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

Q5SM45.

Entry information

Entry name

SYR_THET8

Accession

Primary (citable) accession number: Q5SM45

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 27, 2006
Last sequence update:	December 21, 2004
Last modified:	May 1, 2013
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program