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Protein

GTPase Era

Gene

era

Organism
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

An essential GTPase that binds both GDP and GTP, with rapid nucleotide exchange. Plays a role in 16S rRNA processing and 30S ribosomal subunit biogenesis and possibly also in cell cycle regulation and energy metabolism (By similarity).By similarity
The Era binding site on the 30S subunit overlaps that of protein S1 and is in direct contact with 5 nucleotides directly upstream of the anti-Shine-Dalgarno sequence; the presence of Era may prevent mRNA recruitment. 30S subunits bound to Era are not able to bind to the 50S subunit to make functional ribosomes. Era may serve a role in the final stages of 30S ribosomal subunit assembly.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi14 – 218GTPSequence analysis
Nucleotide bindingi61 – 655GTPSequence analysis
Nucleotide bindingi125 – 1284GTPSequence analysis

GO - Molecular functioni

  • GTPase activity Source: UniProtKB-HAMAP
  • GTP binding Source: UniProtKB-HAMAP
  • ribosomal small subunit binding Source: UniProtKB
  • small ribosomal subunit rRNA binding Source: UniProtKB

GO - Biological processi

  • ribosomal small subunit assembly Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Ribosome biogenesis

Keywords - Ligandi

GTP-binding, Nucleotide-binding, RNA-binding, rRNA-binding

Enzyme and pathway databases

BioCyciTTHE300852:GH8R-127-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
GTPase Era
Gene namesi
Name:era
Ordered Locus Names:TTHA0120
OrganismiThermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Taxonomic identifieri300852 [NCBI]
Taxonomic lineageiBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus
Proteomesi
  • UP000000532 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 301301GTPase EraPRO_0000403972Add
BLAST

Interactioni

Subunit structurei

Monomer (Probable). Binds to the 30S ribosomal subunit, between the head and platform, contacting the 16S rRNA. Contacts ribosomal proteins S7 and S18 and may contact proteins S2 and S11.Curated1 Publication

Protein-protein interaction databases

STRINGi300852.TTHA0120.

Structurei

Secondary structure

1
301
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi6 – 138Combined sources
Helixi20 – 289Combined sources
Beta strandi37 – 393Combined sources
Beta strandi46 – 527Combined sources
Beta strandi55 – 617Combined sources
Helixi71 – 8313Combined sources
Beta strandi88 – 958Combined sources
Helixi102 – 11110Combined sources
Helixi112 – 1143Combined sources
Turni115 – 1173Combined sources
Beta strandi120 – 1256Combined sources
Helixi127 – 1293Combined sources
Helixi133 – 14210Combined sources
Beta strandi147 – 1515Combined sources
Helixi157 – 16812Combined sources
Beta strandi184 – 1874Combined sources
Helixi189 – 20315Combined sources
Helixi209 – 2124Combined sources
Beta strandi214 – 22310Combined sources
Turni224 – 2263Combined sources
Beta strandi227 – 23812Combined sources
Helixi239 – 2468Combined sources
Helixi248 – 2503Combined sources
Helixi251 – 26818Combined sources
Beta strandi270 – 28011Combined sources
Helixi284 – 2863Combined sources
Helixi288 – 2947Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1WF3X-ray1.88A1-301[»]
1X18electron microscopy13.50X1-301[»]
1X1Lelectron microscopy13.50X1-301[»]
ProteinModelPortaliQ5SM23.
SMRiQ5SM23. Positions 3-298.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ5SM23.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini6 – 173168Era-type GAdd
BLAST
Domaini205 – 28278KH type-2Add
BLAST

Sequence similaritiesi

Contains 1 KH type-2 domain.Curated

Phylogenomic databases

eggNOGiENOG4105CWT. Bacteria.
COG1159. LUCA.
HOGENOMiHOG000245597.
KOiK03595.
OMAiKVAKDWQ.
OrthoDBiEOG68DD1Q.
PhylomeDBiQ5SM23.

Family and domain databases

Gene3Di3.30.300.20. 1 hit.
3.40.50.300. 1 hit.
HAMAPiMF_00367. GTPase_Era.
InterProiIPR030388. G_ERA_dom.
IPR005662. GTP-bd_Era.
IPR006073. GTP_binding_domain.
IPR015946. KH_dom-like_a/b.
IPR004044. KH_dom_type_2.
IPR009019. KH_prok-type.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
[Graphical view]
PfamiPF07650. KH_2. 1 hit.
PF01926. MMR_HSR1. 1 hit.
[Graphical view]
PRINTSiPR00326. GTP1OBG.
SUPFAMiSSF52540. SSF52540. 1 hit.
SSF54814. SSF54814. 1 hit.
TIGRFAMsiTIGR00436. era. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEiPS51713. G_ERA. 1 hit.
PS50823. KH_TYPE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5SM23-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAEKTYSGFV AIVGKPNVGK STLLNNLLGV KVAPISPRPQ TTRKRLRGIL
60 70 80 90 100
TEGRRQIVFV DTPGLHKPMD ALGEFMDQEV YEALADVNAV VWVVDLRHPP
110 120 130 140 150
TPEDELVARA LKPLVGKVPI LLVGNKLDAA KYPEEAMKAY HELLPEAEPR
160 170 180 190 200
MLSALDERQV AELKADLLAL MPEGPFFYPE DYAKSDQTFG EWVAEILREE
210 220 230 240 250
AMKRLWHEVP YAVATKVEEV AERENGVLYI KAILYVERPS QKAIVIGEGG
260 270 280 290 300
RKIKEIGQAT RKQLEALLGK KVYLDLEVKV YPDWRKDPEA LRELGYRSSV

G
Length:301
Mass (Da):33,809
Last modified:December 21, 2004 - v1
Checksum:iE5403580A8D161F5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP008226 Genomic DNA. Translation: BAD69943.1.
RefSeqiWP_008630929.1. NC_006461.1.
YP_143386.1. NC_006461.1.

Genome annotation databases

EnsemblBacteriaiBAD69943; BAD69943; BAD69943.
GeneIDi3167951.
KEGGittj:TTHA0120.
PATRICi23955177. VBITheThe93045_0118.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP008226 Genomic DNA. Translation: BAD69943.1.
RefSeqiWP_008630929.1. NC_006461.1.
YP_143386.1. NC_006461.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1WF3X-ray1.88A1-301[»]
1X18electron microscopy13.50X1-301[»]
1X1Lelectron microscopy13.50X1-301[»]
ProteinModelPortaliQ5SM23.
SMRiQ5SM23. Positions 3-298.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi300852.TTHA0120.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAD69943; BAD69943; BAD69943.
GeneIDi3167951.
KEGGittj:TTHA0120.
PATRICi23955177. VBITheThe93045_0118.

Phylogenomic databases

eggNOGiENOG4105CWT. Bacteria.
COG1159. LUCA.
HOGENOMiHOG000245597.
KOiK03595.
OMAiKVAKDWQ.
OrthoDBiEOG68DD1Q.
PhylomeDBiQ5SM23.

Enzyme and pathway databases

BioCyciTTHE300852:GH8R-127-MONOMER.

Miscellaneous databases

EvolutionaryTraceiQ5SM23.

Family and domain databases

Gene3Di3.30.300.20. 1 hit.
3.40.50.300. 1 hit.
HAMAPiMF_00367. GTPase_Era.
InterProiIPR030388. G_ERA_dom.
IPR005662. GTP-bd_Era.
IPR006073. GTP_binding_domain.
IPR015946. KH_dom-like_a/b.
IPR004044. KH_dom_type_2.
IPR009019. KH_prok-type.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
[Graphical view]
PfamiPF07650. KH_2. 1 hit.
PF01926. MMR_HSR1. 1 hit.
[Graphical view]
PRINTSiPR00326. GTP1OBG.
SUPFAMiSSF52540. SSF52540. 1 hit.
SSF54814. SSF54814. 1 hit.
TIGRFAMsiTIGR00436. era. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEiPS51713. G_ERA. 1 hit.
PS50823. KH_TYPE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Complete genome sequence of Thermus thermophilus HB8."
    Masui R., Kurokawa K., Nakagawa N., Tokunaga F., Koyama Y., Shibata T., Oshima T., Yokoyama S., Yasunaga T., Kuramitsu S.
    Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: HB8 / ATCC 27634 / DSM 579.
  2. "Interaction of Era with the 30S ribosomal subunit implications for 30S subunit assembly."
    Sharma M.R., Barat C., Wilson D.N., Booth T.M., Kawazoe M., Hori-Takemoto C., Shirouzu M., Yokoyama S., Fucini P., Agrawal R.K.
    Mol. Cell 18:319-329(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY ELECTRON MICROSCOPY (13.50 ANGSTROMS), INTERACTION WITH 30S RIBOSOMAL SUBUNIT, RIBOSOMAL PROTEINS S2; S7; S11 AND S18, SMALL RRNA-BINDING.
    Strain: HB8 / ATCC 27634 / DSM 579.
  3. "Crystal structure of GTP-binding protein TT1341 from Thermus thermophilus HB8."
    Kawazoe M., Hori-Takemoto C., Kaminishi T., Sekine S., Shirouzu M., Yokoyama S.
    Submitted (FEB-2009) to the PDB data bank
    Cited for: X-RAY CRYSTALLOGRAPHY (1.88 ANGSTROMS) IN COMPLEX WITH A GUANINE NUCLEOTIDE.
    Strain: HB8 / ATCC 27634 / DSM 579.

Entry informationi

Entry nameiERA_THET8
AccessioniPrimary (citable) accession number: Q5SM23
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 11, 2011
Last sequence update: December 21, 2004
Last modified: November 11, 2015
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.