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Protein
Submitted name:

Peptide chain release factor 2

Gene

TTHA0142

Organism
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Status
Unreviewed-Annotation score: Annotation score: 1 out of 5-Experimental evidence at protein leveli

Functioni

Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA.SAAS annotation

GO - Molecular functioni

  1. translation release factor activity, codon specific Source: InterPro
Complete GO annotation...

Keywords - Biological processi

Protein biosynthesisSAAS annotation

Enzyme and pathway databases

BioCyciTTHE300852:GH8R-150-MONOMER.

Names & Taxonomyi

Protein namesi
Submitted name:
Peptide chain release factor 2Imported
Gene namesi
Ordered Locus Names:TTHA0142Imported
OrganismiThermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)Imported
Taxonomic identifieri300852 [NCBI]
Taxonomic lineageiBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus
ProteomesiUP000000532: Chromosome

Subcellular locationi

Cytoplasm SAAS annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

CytoplasmSAAS annotation

Interactioni

Protein-protein interaction databases

STRINGi300852.TTHA0142.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2IHRX-ray2.50127-378[»]
2WH1X-ray3.45Y31-369[»]
2WH3X-ray3.45Y31-369[»]
2X9RX-ray3.10Y31-369[»]
2X9TX-ray3.10Y31-369[»]
ProteinModelPortaliQ5SM01.
SMRiQ5SM01. Positions 27-369.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ5SM01.

Family & Domainsi

Phylogenomic databases

eggNOGiCOG1186.
HOGENOMiHOG000074814.
KOiK02836.
OMAiLESPTIW.
OrthoDBiEOG6TN48J.
PhylomeDBiQ5SM01.

Family and domain databases

Gene3Di3.30.160.20. 1 hit.
InterProiIPR014720. dsRNA-bd_dom.
IPR005139. PCRF.
IPR000352. Pep_chain_release_fac_I_II.
IPR004374. PrfB.
[Graphical view]
PANTHERiPTHR11075:SF6. PTHR11075:SF6. 1 hit.
PfamiPF03462. PCRF. 1 hit.
PF00472. RF-1. 1 hit.
[Graphical view]
SMARTiSM00937. PCRF. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00020. prfB. 1 hit.
PROSITEiPS00745. RF_PROK_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5SM01-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MRLASQSAIL VKVWTWNASR NAWKASGGIF DIPQKETRLK ELERRLEDPS
60 70 80 90 100
LWNDPEAARK VSQEAARLRR TVDTFRSLES DLQGLLELME ELPAEEREAL
110 120 130 140 150
KPELEEAAKK LDELYHQTLL NFPHAEKNAI LTIQPGAGGT EACDWAEMLL
160 170 180 190 200
RMYTRFAERQ GFQVEVVDLT PGPEAGIDYA QILVKGENAY GLLSPEAGVH
210 220 230 240 250
RLVRPSPFDA SGRRHTSFAG VEVIPEVDEE VEVVLKPEEL RIDVMRASGP
260 270 280 290 300
GGQGVNTTDS AVRVVHLPTG ITVTCQTTRS QIKNKELALK ILKARLYELE
310 320 330 340 350
RKKREEELKA LRGEVRPIEW GSQIRSYVLD KNYVKDHRTG LMRHDPENVL
360 370
DGDLMDLIWA GLEWKAGRRQ GTEEVEAE
Length:378
Mass (Da):42,734
Last modified:December 21, 2004 - v1
Checksum:i55B4AC9C891AEEB7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP008226 Genomic DNA. Translation: BAD69965.1.
RefSeqiYP_143408.1. NC_006461.1.

Genome annotation databases

EnsemblBacteriaiBAD69965; BAD69965; BAD69965.
GeneIDi3168831.
KEGGittj:TTHA0142.
PATRICi23955223. VBITheThe93045_0140.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP008226 Genomic DNA. Translation: BAD69965.1.
RefSeqiYP_143408.1. NC_006461.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2IHRX-ray2.50127-378[»]
2WH1X-ray3.45Y31-369[»]
2WH3X-ray3.45Y31-369[»]
2X9RX-ray3.10Y31-369[»]
2X9TX-ray3.10Y31-369[»]
ProteinModelPortaliQ5SM01.
SMRiQ5SM01. Positions 27-369.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi300852.TTHA0142.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAD69965; BAD69965; BAD69965.
GeneIDi3168831.
KEGGittj:TTHA0142.
PATRICi23955223. VBITheThe93045_0140.

Phylogenomic databases

eggNOGiCOG1186.
HOGENOMiHOG000074814.
KOiK02836.
OMAiLESPTIW.
OrthoDBiEOG6TN48J.
PhylomeDBiQ5SM01.

Enzyme and pathway databases

BioCyciTTHE300852:GH8R-150-MONOMER.

Miscellaneous databases

EvolutionaryTraceiQ5SM01.

Family and domain databases

Gene3Di3.30.160.20. 1 hit.
InterProiIPR014720. dsRNA-bd_dom.
IPR005139. PCRF.
IPR000352. Pep_chain_release_fac_I_II.
IPR004374. PrfB.
[Graphical view]
PANTHERiPTHR11075:SF6. PTHR11075:SF6. 1 hit.
PfamiPF03462. PCRF. 1 hit.
PF00472. RF-1. 1 hit.
[Graphical view]
SMARTiSM00937. PCRF. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00020. prfB. 1 hit.
PROSITEiPS00745. RF_PROK_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Complete genome sequence of Thermus thermophilus HB8."
    Masui R., Kurokawa K., Nakagawa N., Tokunaga F., Koyama Y., Shibata T., Oshima T., Yokoyama S., Yasunaga T., Kuramitsu S.
    Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: HB8 / ATCC 27634 / DSM 579Imported.
  2. "Release factors 2 from Escherichia coli and Thermus thermophilus: structural, spectroscopic and microcalorimetric studies."
    Zoldak G., Redecke L., Svergun D.I., Konarev P.V., Voertler C.S., Dobbek H., Sedlak E., Sprinzl M.
    Nucleic Acids Res. 35:1343-1353(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.50 ANGSTROMS) OF 27-378.
  3. "Insights into translational termination from the structure of RF2 bound to the ribosome."
    Weixlbaumer A., Jin H., Neubauer C., Voorhees R.M., Petry S., Kelley A.C., Ramakrishnan V.
    Science 322:953-956(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (3.45 ANGSTROMS) OF 31-369.
  4. "Structure of the 70S ribosome bound to release factor 2 and a substrate analog provides insights into catalysis of peptide release."
    Jin H., Kelley A.C., Loakes D., Ramakrishnan V.
    Proc. Natl. Acad. Sci. U.S.A. 107:8593-8598(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (3.10 ANGSTROMS) OF 31-369.

Entry informationi

Entry nameiQ5SM01_THET8
AccessioniPrimary (citable) accession number: Q5SM01
Entry historyi
Integrated into UniProtKB/TrEMBL: December 21, 2004
Last sequence update: December 21, 2004
Last modified: February 4, 2015
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

3D-structureCombined sources, Complete proteome, Reference proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.