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Protein

2-oxoisovalerate dehydrogenase subunit alpha

Gene

TTHA0229

Organism
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO2. It contains multiple copies of three enzymatic components: branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransferase (E2) and lipoamide dehydrogenase (E3) (By similarity).By similarity

Catalytic activityi

3-methyl-2-oxobutanoate + [dihydrolipoyllysine-residue (2-methylpropanoyl)transferase] lipoyllysine = [dihydrolipoyllysine-residue (2-methylpropanoyl)transferase] S-(2-methylpropanoyl)dihydrolipoyllysine + CO2.1 Publication

Cofactori

thiamine diphosphate1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei66Substrate1 Publication1
Binding sitei95Substrate1 Publication1
Binding sitei144Substrate1 Publication1
Metal bindingi175Magnesium1 Publication1
Metal bindingi204Magnesium1 Publication1
Metal bindingi206Magnesium; via carbonyl oxygen1 Publication1
Binding sitei273Thiamine pyrophosphate1 Publication1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

Magnesium, Metal-binding, Thiamine pyrophosphate

Enzyme and pathway databases

BioCyciTTHE300852:GH8R-237-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
2-oxoisovalerate dehydrogenase subunit alpha (EC:1.2.4.4)
Alternative name(s):
Branched-chain alpha-keto acid dehydrogenase E1 component alpha chain
Short name:
BCKDH E1-alpha
Gene namesi
Ordered Locus Names:TTHA0229
OrganismiThermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Taxonomic identifieri300852 [NCBI]
Taxonomic lineageiBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus
Proteomesi
  • UP000000532 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002949751 – 3672-oxoisovalerate dehydrogenase subunit alphaAdd BLAST367

Interactioni

Subunit structurei

Heterotetramer of two alpha and two beta chains. Directly associated with ODBB in the E1 complex.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
TTHA0230Q5SLR34EBI-1038222,EBI-1038230

Protein-protein interaction databases

IntActiQ5SLR4. 1 interactor.
STRINGi300852.TTHA0229.

Structurei

Secondary structure

1367
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi35 – 60Combined sources26
Helixi74 – 83Combined sources10
Turni86 – 88Combined sources3
Beta strandi89 – 92Combined sources4
Turni95 – 97Combined sources3
Helixi98 – 104Combined sources7
Helixi108 – 116Combined sources9
Turni122 – 125Combined sources4
Turni135 – 138Combined sources4
Turni146 – 149Combined sources4
Helixi150 – 162Combined sources13
Beta strandi169 – 174Combined sources6
Helixi176 – 179Combined sources4
Helixi181 – 192Combined sources12
Beta strandi196 – 203Combined sources8
Beta strandi205 – 207Combined sources3
Helixi212 – 215Combined sources4
Beta strandi217 – 219Combined sources3
Helixi222 – 227Combined sources6
Beta strandi232 – 236Combined sources5
Helixi240 – 255Combined sources16
Beta strandi261 – 266Combined sources6
Helixi279 – 281Combined sources3
Helixi285 – 292Combined sources8
Helixi296 – 305Combined sources10
Turni306 – 308Combined sources3
Helixi312 – 335Combined sources24
Helixi341 – 345Combined sources5
Beta strandi348 – 351Combined sources4
Helixi354 – 366Combined sources13

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1UM9X-ray2.20A/C1-367[»]
1UMBX-ray2.10A/C1-367[»]
1UMCX-ray2.40A/C1-367[»]
1UMDX-ray1.90A/C1-367[»]
ProteinModelPortaliQ5SLR4.
SMRiQ5SLR4.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ5SLR4.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni94 – 96Thiamine pyrophosphate binding1 Publication3
Regioni128 – 131Substrate binding1 Publication4
Regioni144 – 146Thiamine pyrophosphate binding1 Publication3
Regioni174 – 180Thiamine pyrophosphate binding1 Publication7
Regioni204 – 208Thiamine pyrophosphate binding1 Publication5

Sequence similaritiesi

Belongs to the BCKDHA family.Curated

Phylogenomic databases

eggNOGiENOG4108JYK. Bacteria.
COG1071. LUCA.
HOGENOMiHOG000281335.
KOiK00166.
OMAiNEEWEED.
PhylomeDBiQ5SLR4.

Family and domain databases

Gene3Di3.40.50.970. 1 hit.
InterProiIPR001017. DH_E1.
IPR029061. THDP-binding.
[Graphical view]
PfamiPF00676. E1_dh. 1 hit.
[Graphical view]
SUPFAMiSSF52518. SSF52518. 1 hit.

Sequencei

Sequence statusi: Complete.

Q5SLR4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVKETHRFET FTEEPIRLIG EEGEWLGDFP LDLEGEKLRR LYRDMLAARM
60 70 80 90 100
LDERYTILIR TGKTSFIAPA AGHEAAQVAI AHAIRPGFDW VFPYYRDHGL
110 120 130 140 150
ALALGIPLKE LLGQMLATKA DPNKGRQMPE HPGSKALNFF TVASPIASHV
160 170 180 190 200
PPAAGAAISM KLLRTGQVAV CTFGDGATSE GDWYAGINFA AVQGAPAVFI
210 220 230 240 250
AENNFYAISV DYRHQTHSPT IADKAHAFGI PGYLVDGMDV LASYYVVKEA
260 270 280 290 300
VERARRGEGP SLVELRVYRY GPHSSADDDS RYRPKEEVAF WRKKDPIPRF
310 320 330 340 350
RRFLEARGLW NEEWEEDVRE EIRAELERGL KEAEEAGPVP PEWMFEDVFA
360
EKPWHLLRQE ALLKEEL
Length:367
Mass (Da):41,452
Last modified:December 21, 2004 - v1
Checksum:i178B4EFE0CA5F1D1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP008226 Genomic DNA. Translation: BAD70052.1.
RefSeqiWP_011227793.1. NC_006461.1.
YP_143495.1. NC_006461.1.

Genome annotation databases

EnsemblBacteriaiBAD70052; BAD70052; BAD70052.
GeneIDi3168003.
KEGGittj:TTHA0229.
PATRICi23955399. VBITheThe93045_0227.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP008226 Genomic DNA. Translation: BAD70052.1.
RefSeqiWP_011227793.1. NC_006461.1.
YP_143495.1. NC_006461.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1UM9X-ray2.20A/C1-367[»]
1UMBX-ray2.10A/C1-367[»]
1UMCX-ray2.40A/C1-367[»]
1UMDX-ray1.90A/C1-367[»]
ProteinModelPortaliQ5SLR4.
SMRiQ5SLR4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ5SLR4. 1 interactor.
STRINGi300852.TTHA0229.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAD70052; BAD70052; BAD70052.
GeneIDi3168003.
KEGGittj:TTHA0229.
PATRICi23955399. VBITheThe93045_0227.

Phylogenomic databases

eggNOGiENOG4108JYK. Bacteria.
COG1071. LUCA.
HOGENOMiHOG000281335.
KOiK00166.
OMAiNEEWEED.
PhylomeDBiQ5SLR4.

Enzyme and pathway databases

BioCyciTTHE300852:GH8R-237-MONOMER.

Miscellaneous databases

EvolutionaryTraceiQ5SLR4.

Family and domain databases

Gene3Di3.40.50.970. 1 hit.
InterProiIPR001017. DH_E1.
IPR029061. THDP-binding.
[Graphical view]
PfamiPF00676. E1_dh. 1 hit.
[Graphical view]
SUPFAMiSSF52518. SSF52518. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiODBA_THET8
AccessioniPrimary (citable) accession number: Q5SLR4
Secondary accession number(s): P84129
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 10, 2007
Last sequence update: December 21, 2004
Last modified: November 2, 2016
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.