Q5SLR4 (ODBA_THET8) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
2-oxoisovalerate dehydrogenase subunit alpha
EC=1.2.4.4

Alternative name(s):
Branched-chain alpha-keto acid dehydrogenase E1 component alpha chain
Short name=BCKDH E1-alpha

Gene names

Ordered Locus Names:

TTHA0229

Organism

Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) [Reference proteome] [HAMAP]

Taxonomic identifier

300852 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Deinococcus-Thermus › Deinococci › Thermales › Thermaceae › Thermus › Thermus thermophilus

Protein attributes

Sequence length	367 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO2. It contains multiple copies of three enzymatic components: branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransferase (E2) and lipoamide dehydrogenase (E3) By similarity. UniProtKB P37940
Catalytic activity	3-methyl-2-oxobutanoate + [dihydrolipoyllysine-residue (2-methylpropanoyl)transferase] lipoyllysine = [dihydrolipoyllysine-residue (2-methylpropanoyl)transferase] S-(2-methylpropanoyl)dihydrolipoyllysine + CO2. Ref.2
Cofactor	Thiamine pyrophosphate. Ref.2
Subunit structure	Heterotetramer of two alpha and two beta chains. Directly associated with ODBB in the E1 complex. Ref.2
Sequence similarities	Belongs to the BCKDHA family.

Ontologies

Keywords
Ligand	Magnesium Metal-binding Thiamine pyrophosphate
Molecular function	Oxidoreductase
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Molecular_function	3-methyl-2-oxobutanoate dehydrogenase (2-methylpropanoyl-transferring) activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
TTHA0230	Q5SLR3	4	EBI-1038222,EBI-1038230

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 367

367

2-oxoisovalerate dehydrogenase subunit alpha

PRO_0000294975

Regions

Region

94 – 96

3

Thiamine pyrophosphate binding Ref.2

Region

128 – 131

4

Substrate binding Ref.2

Region

144 – 146

3

Thiamine pyrophosphate binding Ref.2

Region

174 – 180

7

Thiamine pyrophosphate binding Ref.2

Region

204 – 208

5

Thiamine pyrophosphate binding Ref.2

Sites

Metal binding

175

1

Magnesium Ref.2

Metal binding

204

1

Magnesium Ref.2

Metal binding

206

1

Magnesium; via carbonyl oxygen Ref.2

Binding site

66

1

Substrate Ref.2

Binding site

95

1

Substrate Ref.2

Binding site

144

1

Substrate Ref.2

Binding site

273

1

Thiamine pyrophosphate Ref.2

Secondary structure

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367

Helix Strand Turn

Details...

Helix

35 – 60

26

Helix

74 – 83

10

Turn

86 – 88

3

Beta strand

89 – 92

4

Turn

95 – 97

3

Helix

98 – 104

7

Helix

108 – 116

9

Turn

122 – 125

4

Turn

135 – 138

4

Turn

146 – 149

4

Helix

150 – 162

13

Beta strand

169 – 174

6

Helix

176 – 179

4

Helix

181 – 192

12

Beta strand

196 – 203

8

Beta strand

205 – 207

3

Helix

212 – 215

4

Beta strand

217 – 219

3

Helix

222 – 227

6

Beta strand

232 – 236

5

Helix

240 – 255

16

Beta strand

261 – 266

6

Helix

279 – 281

3

Helix

285 – 292

8

Helix

296 – 305

10

Turn

306 – 308

3

Helix

312 – 335

24

Helix

341 – 345

5

Beta strand

348 – 351

4

Helix

354 – 366

13

Sequences

	Sequence		Length	Mass (Da)
	Q5SLR4 [UniParc]. Last modified December 21, 2004. Version 1. Checksum: 178B4EFE0CA5F1D1	FASTA	367	41,452
	10 20 30 40 50 60 MVKETHRFET FTEEPIRLIG EEGEWLGDFP LDLEGEKLRR LYRDMLAARM LDERYTILIR 70 80 90 100 110 120 TGKTSFIAPA AGHEAAQVAI AHAIRPGFDW VFPYYRDHGL ALALGIPLKE LLGQMLATKA 130 140 150 160 170 180 DPNKGRQMPE HPGSKALNFF TVASPIASHV PPAAGAAISM KLLRTGQVAV CTFGDGATSE 190 200 210 220 230 240 GDWYAGINFA AVQGAPAVFI AENNFYAISV DYRHQTHSPT IADKAHAFGI PGYLVDGMDV 250 260 270 280 290 300 LASYYVVKEA VERARRGEGP SLVELRVYRY GPHSSADDDS RYRPKEEVAF WRKKDPIPRF 310 320 330 340 350 360 RRFLEARGLW NEEWEEDVRE EIRAELERGL KEAEEAGPVP PEWMFEDVFA EKPWHLLRQE ALLKEEL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete genome sequence of Thermus thermophilus HB8." Masui R., Kurokawa K., Nakagawa N., Tokunaga F., Koyama Y., Shibata T., Oshima T., Yokoyama S., Yasunaga T., Kuramitsu S. Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: HB8 / ATCC 27634 / DSM 579.
[2]	"Ligand-induced conformational changes and a reaction intermediate in branched-chain 2-oxo acid dehydrogenase (E1) from Thermus thermophilus HB8, as revealed by X-ray crystallography." Nakai T., Nakagawa N., Maoka N., Masui R., Kuramitsu S., Kamiya N. J. Mol. Biol. 337:1011-1033(2004) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS) IN COMPLEX WITH ODBB AND THIAMINE PYROPHOSPHATE, CATALYTIC ACTIVITY, COFACTOR, SUBUNIT.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AP008226 Genomic DNA. Translation: BAD70052.1.

RefSeq

YP_143495.1. NC_006461.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1UM9	X-ray	2.20	A/C	1-367	[»]
1UMB	X-ray	2.10	A/C	1-367	[»]
1UMC	X-ray	2.40	A/C	1-367	[»]
1UMD	X-ray	1.90	A/C	1-367	[»]

ProteinModelPortal

Q5SLR4.

SMR

Q5SLR4. Positions 6-367.

ModBase

Search...

Protein-protein interaction databases

IntAct

Q5SLR4. 1 interaction.

STRING

300852.TTHA0229.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

BAD70052; BAD70052; BAD70052.

GeneID

3168003.

KEGG

ttj:TTHA0229.

PATRIC

23955399. VBITheThe93045_0227.

Phylogenomic databases

eggNOG

COG1071.

HOGENOM

HOG000281335.

KO

K00166.

OMA

ILIRTGK.

ProtClustDB

CLSK2762003.

Family and domain databases

InterPro

IPR001017. DH_E1.
[Graphical view]

Pfam

PF00676. E1_dh. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

Q5SLR4.

Entry information

Entry name

ODBA_THET8

Accession

Primary (citable) accession number: Q5SLR4
Secondary accession number(s): P84129

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 10, 2007
Last sequence update:	December 21, 2004
Last modified:	May 1, 2013
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program