3-hydroxyisobutyrate dehydrogenase - Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)

Preferred order of sections

Names and origin

Protein names

Submitted name:
3-hydroxyisobutyrate dehydrogenase EMBL BAD70060.1

Gene names

Ordered Locus Names:

TTHA0237

Organism

Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)

Taxonomic identifier

300852 [NCBI]

Taxonomic lineage

Bacteria › Deinococcus-Thermus › Deinococci › Thermales › Thermaceae › Thermus

Protein attributes

Sequence length	289 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

Ontologies

Keywords
Ligand	NADP PDB 1WP4 PDB 2CVZ Nucleotide-binding PDB 1WP4 PDB 2CVZ
Technical term	3D-structure PDB 1WP4 PDB 2CVZ Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	pentose-phosphate shunt Inferred from electronic annotation. Source: InterPro valine metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	3-hydroxyisobutyrate dehydrogenase activity Inferred from electronic annotation. Source: InterPro coenzyme binding Inferred from electronic annotation. Source: InterPro nucleotide binding Inferred from electronic annotation. Source: UniProtKB-KW phosphogluconate dehydrogenase (decarboxylating) activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Regions

Nucleotide binding

9 – 12

4

NADP PDB 1WP4 PDB 2CVZ

Nucleotide binding

30 – 35

6

NADP PDB 1WP4 PDB 2CVZ

Nucleotide binding

63 – 64

2

NADP PDB 1WP4 PDB 2CVZ

Nucleotide binding

226 – 227

2

NADP PDB 1WP4 PDB 2CVZ

Sites

Binding site

71

1

NADP PDB 1WP4 PDB 2CVZ

Binding site

91

1

NADP PDB 1WP4 PDB 2CVZ

Binding site

234

1

NADP PDB 1WP4 PDB 2CVZ

Sequences

Sequence

Length

Mass (Da)

Tools

Q5SLQ6 [UniParc].

Last modified December 21, 2004. Version 1.
Checksum: 8C2B876654903757
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FASTA

289

31,340

        10         20         30         40         50         60 
MEKVAFIGLG AMGYPMAGHL ARRFPTLVWN RTFEKALRHQ EEFGSEAVPL ERVAEARVIF 

        70         80         90        100        110        120 
TCLPTTREVY EVAEALYPYL REGTYWVDAT SGEPEASRRL AERLREKGVT YLDAPVSGGT 

       130        140        150        160        170        180 
SGAEAGTLTV MLGGPEEAVE RVRPFLAYAK KVVHVGPVGA GHAVKAINNA LLAVNLWAAG 

       190        200        210        220        230        240 
EGLLALVKQG VSAEKALEVI NASSGRSNAT ENLIPQRVLT RAFPKTFALG LLVKDLGIAM 

       250        260        270        280 
GVLDGEKAPS PLLRLAREVY EMAKRELGPD ADHVEALRLL ERWGGVEIR

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete genome sequence of Thermus thermophilus HB8." Masui R., Kurokawa K., Nakagawa N., Tokunaga F., Koyama Y., Shibata T., Oshima T., Yokoyama S., Yasunaga T., Kuramitsu S. Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: HB8 / ATCC 27634 / DSM 579.
[2]	"Crystal structure of novel NADP-dependent 3-hydroxyisobutyrate dehydrogenase from Thermus thermophilus HB8." Lokanath N.K., Ohshima N., Takio K., Shiromizu I., Kuroishi C., Okazaki N., Kuramitsu S., Yokoyama S., Miyano M., Kunishima N. J. Mol. Biol. 352:905-917(2005) [PubMed: 16126223] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.80 ANGSTROMS) IN COMPLEX WITH NADP.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AP008226 Genomic DNA. Translation: BAD70060.1.

RefSeq

YP_143503.1. NC_006461.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1WP4	X-ray	2.00	A/B/C/D	1-289	[»]
2CVZ	X-ray	1.80	A/B/C/D	1-289	[»]

ProteinModelPortal

Q5SLQ6.

SMR

Q5SLQ6. Positions 1-289.

ModBase

Search...

Protein-protein interaction databases

STRING

Q5SLQ6.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

GeneID

3168163.

GenomeReviews

Gene locus TTHA0237 in contig AP008226_GR.

KEGG

ttj:TTHA0237.

PATRIC

23955419. VBITheThe93045_0237.

Phylogenomic databases

eggNOG

COG2084.

HOGENOM

HBG729179.

OMA

VLTREFP.

PhylomeDB

Q5SLQ6.

ProtClustDB

CLSK799224.

Enzyme and pathway databases

BioCyc

TTHE300852:TTHA0237-MONOMER.

Family and domain databases

InterPro

IPR002204. 3-OH-isobutyrate_DH-rel_CS.
IPR015815. 3hydroxyacid_DH/Rdtase.
IPR008927. 6-PGluconate_DH_C-like.
IPR006115. 6PGDH_NADP-bd.
IPR013328. DH_multihelical.
IPR016040. NAD(P)-bd_dom.
[Graphical view]

Gene3D

G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
G3DSA:1.10.1040.10. Opine_DH. 1 hit.

KO

K00020.

PANTHER

PTHR22981. 3hydroxy_acid_DH. 1 hit.

Pfam

PF03446. NAD_binding_2. 1 hit.
[Graphical view]

SUPFAM

SSF48179. 6DGDH_C_like. 1 hit.

PROSITE

PS00895. 3_HYDROXYISOBUT_DH. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

Q5SLQ6_THET8

Accession

Primary (citable) accession number: Q5SLQ6

Entry history

Integrated into UniProtKB/TrEMBL:	December 21, 2004
Last sequence update:	December 21, 2004
Last modified:	December 14, 2011
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)