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Q5SLP7 (RL1_THET8) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 86. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
50S ribosomal protein L1
Gene names
Name:rplA
Ordered Locus Names:TTHA0246
OrganismThermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) [Reference proteome] [HAMAP]
Taxonomic identifier300852 [NCBI]
Taxonomic lineageBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus

Protein attributes

Sequence length229 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Directly binds to 23S rRNA. Forms what is known as the L1 stalk, which protrudes beyond the 70S ribosome surface. The stalk is preferentially stabilized in 70S versus 50S crystals. Interacts with the E site tRNA, blocking the exit path. This blockage implies that this section of the ribosome must be able to move to release the deacetylated tRNA. HAMAP-Rule MF_01318_B

Protein L1 is also a translational repressor protein, it controls the translation of the L11 operon by binding to its mRNA By similarity. HAMAP-Rule MF_01318_B

Subunit structure

Part of the 50S ribosomal subunit.

Sequence similarities

Belongs to the ribosomal protein L1P family.

Mass spectrometry

Molecular mass is 24698 Da from positions 2 - 229. Determined by MALDI. Ref.4

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.2
Chain2 – 22922850S ribosomal protein L1 HAMAP-Rule MF_01318_B
PRO_0000125764

Secondary structure

........................................................ 229
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q5SLP7 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: 99EE7BE00801B1B4

FASTA22924,831
        10         20         30         40         50         60 
MPKHGKRYRA LLEKVDPNKV YTIDEAARLV KELATAKFDE TVEVHAKLGI DPRRSDQNVR 

        70         80         90        100        110        120 
GTVSLPHGLG KQVRVLAIAK GEKIKEAEEA GADYVGGEEI IQKILDGWMD FDAVVATPDV 

       130        140        150        160        170        180 
MGAVGSKLGR ILGPRGLLPN PKAGTVGFNI GEIIREIKAG RIEFRNDKTG AIHAPVGKAS 

       190        200        210        220 
FPPEKLADNI RAFIRALEAH KPEGAKGTFL RSVYVTTTMG PSVRINPHS 

« Hide

References

« Hide 'large scale' references
[1]"Complete genome sequence of Thermus thermophilus HB8."
Masui R., Kurokawa K., Nakagawa N., Tokunaga F., Koyama Y., Shibata T., Oshima T., Yokoyama S., Yasunaga T., Kuramitsu S.
Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: HB8 / ATCC 27634 / DSM 579.
[2]"Identification of the 50S ribosomal proteins from the eubacterium Thermus thermophilus."
Katsani K.R., Tsiboli P., Anagnostopoulos K., Urlaub H., Choli-Papadopoulou T.
Biol. Chem. 381:1079-1087(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 2-26.
[3]"Identification of the gene encoding transcription factor NusG of Thermus thermophilus."
Heinrich T., Schroeder W., Erdmann V.A., Hartmann R.K.
J. Bacteriol. 174:7859-7863(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 94-181.
Strain: HB8 / ATCC 27634 / DSM 579.
[4]"Extending ribosomal protein identifications to unsequenced bacterial strains using matrix-assisted laser desorption/ionization mass spectrometry."
Suh M.-J., Hamburg D.M., Gregory S.T., Dahlberg A.E., Limbach P.A.
Proteomics 5:4818-4831(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: MASS SPECTROMETRY.
[5]"The path of messenger RNA through the ribosome."
Yusupova G.Z., Yusupov M.M., Cate J.H.D., Noller H.F.
Cell 106:233-241(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (5.0 ANGSTROMS) OF THE RIBOSOME.
[6]"Crystal structure of the ribosome at 5.5 A resolution."
Yusupov M.M., Yusupova G.Z., Baucom A., Lieberman K., Earnest T.N., Cate J.H.D., Noller H.F.
Science 292:883-896(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (5.5 ANGSTROMS) OF THE RIBOSOME.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AP008226 Genomic DNA. Translation: BAD70069.1.
RefSeqYP_143512.1. NC_006461.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1GIYX-ray5.50C2-229[»]
1YL3X-ray5.50C2-229[»]
2HGJX-ray5.00C1-229[»]
2HGQX-ray5.50C1-229[»]
2HGUX-ray4.51C1-229[»]
2J01X-ray2.80C1-229[»]
2J03X-ray2.80C1-229[»]
2OM7electron microscopy7.30K1-229[»]
2V47X-ray3.80C2-228[»]
2V49X-ray3.80C2-228[»]
2WDIX-ray3.30C1-229[»]
2WDJX-ray3.30C1-229[»]
2WDLX-ray3.50C1-229[»]
2WDNX-ray3.50C1-229[»]
2WH2X-ray3.45C1-229[»]
2WH4X-ray3.45C1-229[»]
2WRJX-ray3.60C1-229[»]
2WRLX-ray3.60C1-229[»]
2WROX-ray3.60C1-229[»]
2WRRX-ray3.60C1-229[»]
2X9SX-ray3.10C1-229[»]
2X9UX-ray3.10C1-229[»]
2XG0X-ray3.20C1-229[»]
2XG2X-ray3.20C1-229[»]
2XQEX-ray3.10C1-229[»]
2XTGelectron microscopy7.80C1-229[»]
2XUXelectron microscopy7.60C1-229[»]
2Y0VX-ray3.10C1-229[»]
2Y0XX-ray3.10C1-229[»]
2Y0ZX-ray3.10C1-229[»]
2Y11X-ray3.10C1-229[»]
2Y13X-ray3.10C1-229[»]
2Y15X-ray3.10C1-229[»]
2Y17X-ray3.10C1-229[»]
2Y19X-ray3.10C1-229[»]
3FINelectron microscopy6.40C35-225[»]
3HUXX-ray3.10C1-229[»]
3HUZX-ray3.10C1-229[»]
3KIRX-ray3.30C1-229[»]
3KITX-ray3.30C1-229[»]
3KIWX-ray3.60C1-229[»]
3KIYX-ray3.60C1-229[»]
3KNIX-ray3.00C1-229[»]
3KNKX-ray3.00C1-229[»]
3KNMX-ray3.45C1-229[»]
3KNOX-ray3.45C1-229[»]
3TG8X-ray1.95A1-229[»]
3V6WX-ray3.90C1-229[»]
3V6XX-ray3.90C1-229[»]
3ZN9X-ray3.10C1-229[»]
3ZNEX-ray3.10C1-229[»]
3ZVPX-ray3.80C1-229[»]
4ABSX-ray3.10C1-229[»]
4B8GX-ray3.70C1-229[»]
4B8IX-ray3.70C1-229[»]
4BTDX-ray2.95C2-229[»]
4BYCX-ray3.35C1-229[»]
4BYEX-ray3.35C1-229[»]
4EJBX-ray3.52C1-229[»]
4EJCX-ray3.52C1-229[»]
4JUXX-ray2.86C1-229[»]
4K0MX-ray3.30C19-102[»]
4K0QX-ray3.30C19-102[»]
ProteinModelPortalQ5SLP7.
SMRQ5SLP7. Positions 2-229.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING300852.TTHA0246.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAD70069; BAD70069; BAD70069.
GeneID3168861.
KEGGttj:TTHA0246.
PATRIC23955439. VBITheThe93045_0246.

Phylogenomic databases

eggNOGCOG0081.
HOGENOMHOG000207015.
KOK02863.
OMANEGWTDF.
OrthoDBEOG6FBX2G.
ProtClustDBPRK05424.

Enzyme and pathway databases

BioCycTTHE300852:GH8R-256-MONOMER.

Family and domain databases

Gene3D3.30.190.20. 2 hits.
3.40.50.790. 1 hit.
HAMAPMF_01318_B. Ribosomal_L1_B.
InterProIPR005878. Ribosom_L1_bac-type.
IPR002143. Ribosomal_L1.
IPR023674. Ribosomal_L1-like.
IPR028364. Ribosomal_L1/biogenesis.
IPR016094. Ribosomal_L1_2-a/b-sand.
IPR016095. Ribosomal_L1_3-a/b-sand.
IPR023673. Ribosomal_L1_CS.
[Graphical view]
PfamPF00687. Ribosomal_L1. 1 hit.
[Graphical view]
PIRSFPIRSF002155. Ribosomal_L1. 1 hit.
SUPFAMSSF56808. SSF56808. 1 hit.
TIGRFAMsTIGR01169. rplA_bact. 1 hit.
PROSITEPS01199. RIBOSOMAL_L1. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceQ5SLP7.

Entry information

Entry nameRL1_THET8
AccessionPrimary (citable) accession number: Q5SLP7
Entry history
Integrated into UniProtKB/Swiss-Prot: March 29, 2005
Last sequence update: January 23, 2007
Last modified: February 19, 2014
This is version 86 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Ribosomal proteins

Ribosomal proteins families and list of entries

PDB cross-references

Index of Protein Data Bank (PDB) cross-references