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Protein

50S ribosomal protein L1

Gene

rplA

Organism
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Directly binds to 23S rRNA. Forms what is known as the L1 stalk, which protrudes beyond the 70S ribosome surface. The stalk is preferentially stabilized in 70S versus 50S crystals. Interacts with the E site tRNA, blocking the exit path. This blockage implies that this section of the ribosome must be able to move to release the deacetylated tRNA.
Protein L1 is also a translational repressor protein, it controls the translation of the L11 operon by binding to its mRNA.By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionRepressor, Ribonucleoprotein, Ribosomal protein, RNA-binding, rRNA-binding, tRNA-binding
Biological processTranslation regulation

Enzyme and pathway databases

BioCyciTTHE300852:G1GKC-255-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
50S ribosomal protein L1
Gene namesi
Name:rplA
Ordered Locus Names:TTHA0246
OrganismiThermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Taxonomic identifieri300852 [NCBI]
Taxonomic lineageiBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus
Proteomesi
  • UP000000532 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001257642 – 22950S ribosomal protein L1Add BLAST228

Interactioni

Subunit structurei

Part of the 50S ribosomal subunit.

Protein-protein interaction databases

STRINGi300852.TTHA0246

Structurei

Secondary structure

1229
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi23 – 32Combined sources10
Beta strandi36 – 38Combined sources3
Beta strandi41 – 50Combined sources10
Helixi55 – 57Combined sources3
Beta strandi60 – 64Combined sources5
Helixi70 – 72Combined sources3
Beta strandi75 – 78Combined sources4
Helixi82 – 89Combined sources8
Beta strandi93 – 96Combined sources4
Helixi98 – 100Combined sources3
Helixi101 – 105Combined sources5
Beta strandi112 – 116Combined sources5
Helixi118 – 120Combined sources3
Helixi121 – 132Combined sources12
Turni133 – 136Combined sources4
Helixi141 – 143Combined sources3
Beta strandi146 – 148Combined sources3
Helixi150 – 157Combined sources8
Beta strandi161 – 165Combined sources5
Beta strandi170 – 178Combined sources9
Helixi183 – 199Combined sources17
Beta strandi209 – 217Combined sources9
Beta strandi222 – 225Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2OM7electron microscopy7.30K1-229[»]
3TG8X-ray1.95A1-229[»]
4V42X-ray5.50BC2-229[»]
4V4PX-ray5.50AC2-229[»]
4V4XX-ray5.00BC1-229[»]
4V4YX-ray5.50BC1-229[»]
4V4ZX-ray4.51BC1-229[»]
4V51X-ray2.80BC/DC2-229[»]
4V5AX-ray3.50BC/DC2-229[»]
4V5CX-ray3.30BC/DC1-229[»]
4V5DX-ray3.50BC/DC1-229[»]
4V5EX-ray3.45BC/DC1-229[»]
4V5FX-ray3.60BC/DC1-229[»]
4V5GX-ray3.60BC/DC1-229[»]
4V5JX-ray3.10BC/DC1-229[»]
4V5KX-ray3.20BC/DC1-229[»]
4V5LX-ray3.10BC1-229[»]
4V5Melectron microscopy7.80BC1-229[»]
4V5Nelectron microscopy7.60BC1-229[»]
4V5PX-ray3.10BC/DC1-229[»]
4V5QX-ray3.10BC/DC1-229[»]
4V5RX-ray3.10BC/DC1-229[»]
4V5SX-ray3.10BC/DC1-229[»]
4V68electron microscopy6.40BC19-225[»]
4V6AX-ray3.10BC/DC1-229[»]
4V7JX-ray3.30AC/BC1-229[»]
4V7KX-ray3.60AC/BC1-229[»]
4V7LX-ray3.00BC/DC1-229[»]
4V7MX-ray3.45BC/DC1-229[»]
4V8JX-ray3.90BC/DC1-229[»]
4V8NX-ray3.10BC/DC1-229[»]
4V8OX-ray3.80BC1-229[»]
4V8QX-ray3.10AC1-229[»]
4V8UX-ray3.70BC/DC1-229[»]
4V8XX-ray3.35BC/DC1-229[»]
4V90X-ray2.95BC2-229[»]
4V97X-ray3.52BC/DC1-229[»]
4V9HX-ray2.86BC1-229[»]
4WF1X-ray3.09B519-225[»]
4WPOX-ray2.80AC/CC2-229[»]
4WQFX-ray2.80AC/CC2-229[»]
4WQUX-ray2.80AC/CC2-229[»]
4WQYX-ray2.80AC/CC2-229[»]
4WT8X-ray3.40CA/DA19-111[»]
4WU1X-ray3.2071/791-229[»]
4WZDX-ray3.1071/791-229[»]
4WZOX-ray3.30711-229[»]
5A9Zelectron microscopy4.70AC2-229[»]
5AA0electron microscopy5.00AC2-229[»]
5E7KX-ray3.2071/791-229[»]
5E81X-ray2.9571/791-229[»]
5EL4X-ray3.15711-229[»]
5EL5X-ray3.15711-229[»]
5EL6X-ray3.1071/791-229[»]
5EL7X-ray3.1571/791-229[»]
5HAUX-ray3.001C/2C1-229[»]
5OT7electron microscopy3.80f3-229[»]
5UQ7electron microscopy3.50C3-229[»]
5UQ8electron microscopy3.20C3-229[»]
6C5LX-ray3.20BC/DC1-229[»]
ProteinModelPortaliQ5SLP7
SMRiQ5SLP7
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ5SLP7

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105C64 Bacteria
COG0081 LUCA
HOGENOMiHOG000207015
KOiK02863
OMAiGWTDVDV
PhylomeDBiQ5SLP7

Family and domain databases

CDDicd00403 Ribosomal_L1, 1 hit
Gene3Di3.40.50.790, 1 hit
HAMAPiMF_01318_B Ribosomal_L1_B, 1 hit
InterProiView protein in InterPro
IPR005878 Ribosom_L1_bac-type
IPR002143 Ribosomal_L1
IPR023674 Ribosomal_L1-like
IPR028364 Ribosomal_L1/biogenesis
IPR016095 Ribosomal_L1_3-a/b-sand
IPR023673 Ribosomal_L1_CS
PfamiView protein in Pfam
PF00687 Ribosomal_L1, 1 hit
PIRSFiPIRSF002155 Ribosomal_L1, 1 hit
SUPFAMiSSF56808 SSF56808, 1 hit
TIGRFAMsiTIGR01169 rplA_bact, 1 hit
PROSITEiView protein in PROSITE
PS01199 RIBOSOMAL_L1, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q5SLP7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPKHGKRYRA LLEKVDPNKV YTIDEAARLV KELATAKFDE TVEVHAKLGI
60 70 80 90 100
DPRRSDQNVR GTVSLPHGLG KQVRVLAIAK GEKIKEAEEA GADYVGGEEI
110 120 130 140 150
IQKILDGWMD FDAVVATPDV MGAVGSKLGR ILGPRGLLPN PKAGTVGFNI
160 170 180 190 200
GEIIREIKAG RIEFRNDKTG AIHAPVGKAS FPPEKLADNI RAFIRALEAH
210 220
KPEGAKGTFL RSVYVTTTMG PSVRINPHS
Length:229
Mass (Da):24,831
Last modified:January 23, 2007 - v3
Checksum:i99EE7BE00801B1B4
GO

Mass spectrometryi

Molecular mass is 24698 Da from positions 2 - 229. Determined by MALDI. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP008226 Genomic DNA Translation: BAD70069.1
RefSeqiWP_011227804.1, NC_006461.1
YP_143512.1, NC_006461.1

Genome annotation databases

EnsemblBacteriaiBAD70069; BAD70069; BAD70069
GeneIDi3168861
KEGGittj:TTHA0246
PATRICifig|300852.9.peg.246

Similar proteinsi

Entry informationi

Entry nameiRL1_THET8
AccessioniPrimary (citable) accession number: Q5SLP7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 29, 2005
Last sequence update: January 23, 2007
Last modified: April 25, 2018
This is version 115 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome
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Main funding by: National Institutes of Health