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Q5SLP6

- RL11_THET8

UniProt

Q5SLP6 - RL11_THET8

Protein

50S ribosomal protein L11

Gene

rplK

Organism
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 83 (01 Oct 2014)
      Sequence version 1 (21 Dec 2004)
      Previous versions | rss
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    Functioni

    Forms part of the ribosomal stalk which helps the ribosome interact with GTP-bound translation factors. The stalk extends beyond the surface of the 70S ribosome and is preferentially stabilized in 70S versus 50S crystals.
    In the 70S ribosome is in a position where it could interact transiently with the A site tRNA during translation.

    GO - Molecular functioni

    1. large ribosomal subunit rRNA binding Source: UniProtKB-HAMAP
    2. structural constituent of ribosome Source: InterPro

    GO - Biological processi

    1. translation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Ribonucleoprotein, Ribosomal protein

    Keywords - Ligandi

    RNA-binding, rRNA-binding

    Enzyme and pathway databases

    BioCyciTTHE300852:GH8R-257-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    50S ribosomal protein L11UniRule annotation
    Gene namesi
    Name:rplKUniRule annotation
    Ordered Locus Names:TTHA0247
    OrganismiThermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
    Taxonomic identifieri300852 [NCBI]
    Taxonomic lineageiBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus
    ProteomesiUP000000532: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    1. ribosome Source: UniProtKB-KW

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 14714750S ribosomal protein L11PRO_0000104399Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei3 – 31N6,N6,N6-trimethyllysine2 Publications

    Post-translational modificationi

    The protein probably contains twelve methyl groups; at least one lysine residue (Lys-3) is known to be trimethylated. Methylation of the protein is not required for function.2 Publications

    Keywords - PTMi

    Methylation

    Interactioni

    Subunit structurei

    Part of the ribosomal stalk of the 50S ribosomal subunit. Interacts with L10 and the large rRNA to form the base of the stalk. L10 forms an elongated spine to which 3 L12 dimers bind in a sequential fashion forming a heptameric L10(L12)2(L12)2(L12)2 complex. Contacts one of the L7 dimers.

    Protein-protein interaction databases

    IntActiQ5SLP6. 1 interaction.
    MINTiMINT-4299414.
    STRINGi300852.TTHA0247.

    Structurei

    Secondary structure

    1
    147
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi4 – 1310
    Turni20 – 223
    Helixi23 – 286
    Turni29 – 313
    Helixi34 – 4411
    Turni45 – 473
    Beta strandi52 – 609
    Beta strandi61 – 633
    Beta strandi65 – 695
    Helixi74 – 774
    Turni81 – 833
    Turni91 – 933
    Helixi103 – 1108
    Turni111 – 1144
    Beta strandi118 – 1214
    Helixi123 – 1297
    Turni130 – 1356

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1GIYX-ray5.50L9-116[»]
    1YL3X-ray5.50L6-116[»]
    2B9PX-ray6.46K1-141[»]
    2HGJX-ray5.00L1-147[»]
    2HGQX-ray5.50L1-147[»]
    2HGUX-ray4.51L1-147[»]
    2J01X-ray2.80K1-147[»]
    2J03X-ray2.80K1-147[»]
    2WH2X-ray3.45K1-147[»]
    2WH4X-ray3.45K1-147[»]
    2WRJX-ray3.60K1-147[»]
    2WRLX-ray3.60K1-147[»]
    2X9SX-ray3.10K1-147[»]
    2X9UX-ray3.10K1-147[»]
    2XTGelectron microscopy7.80K1-147[»]
    2XUXelectron microscopy7.60K1-147[»]
    3EGVX-ray1.75B2-147[»]
    3FINelectron microscopy6.40L2-139[»]
    3I8IX-ray3.10L1-147[»]
    4JUXX-ray2.86K1-147[»]
    ProteinModelPortaliQ5SLP6.
    SMRiQ5SLP6. Positions 1-147.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ5SLP6.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the ribosomal protein L11P family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0080.
    HOGENOMiHOG000082123.
    KOiK02867.
    OMAiIEAYIKL.
    OrthoDBiEOG69PQ9D.
    PhylomeDBiQ5SLP6.

    Family and domain databases

    Gene3Di1.10.10.250. 1 hit.
    3.30.1550.10. 1 hit.
    HAMAPiMF_00736. Ribosomal_L11.
    InterProiIPR000911. Ribosomal_L11/L12.
    IPR006519. Ribosomal_L11_bac-typ.
    IPR020783. Ribosomal_L11_C.
    IPR020785. Ribosomal_L11_CS.
    IPR020784. Ribosomal_L11_N.
    [Graphical view]
    PANTHERiPTHR11661. PTHR11661. 1 hit.
    PfamiPF00298. Ribosomal_L11. 1 hit.
    PF03946. Ribosomal_L11_N. 1 hit.
    [Graphical view]
    SMARTiSM00649. RL11. 1 hit.
    [Graphical view]
    SUPFAMiSSF46906. SSF46906. 1 hit.
    SSF54747. SSF54747. 1 hit.
    TIGRFAMsiTIGR01632. L11_bact. 1 hit.
    PROSITEiPS00359. RIBOSOMAL_L11. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q5SLP6-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKKVVAVVKL QLPAGKATPA PPVGPALGQH GANIMEFVKA FNAATANMGD    50
    AIVPVEITIY ADRSFTFVTK TPPASYLIRK AAGLEKGAHK PGREKVGRIT 100
    WEQVLEIAKQ KMPDLNTTDL EAAARMIAGS ARSMGVEVVG APEVKDA 147
    Length:147
    Mass (Da):15,505
    Last modified:December 21, 2004 - v1
    Checksum:i3D8DECEBE85B5FE9
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti20 – 201A → G AA sequence (PubMed:11154066)Curated
    Sequence conflicti23 – 231V → G in L10348. (PubMed:1447157)Curated

    Mass spectrometryi

    Molecular mass is 15506.2 Da from positions 1 - 147. Determined by MALDI. Weight of the unmethylated protein.1 Publication
    Molecular mass is 15675.1 Da from positions 1 - 147. Determined by MALDI. Weight of the methylated protein.1 Publication
    Molecular mass is 15678 Da from positions 1 - 147. Determined by MALDI. 1 Publication

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AP008226 Genomic DNA. Translation: BAD70070.1.
    L10348 Genomic DNA. No translation available.
    RefSeqiYP_143513.1. NC_006461.1.

    Genome annotation databases

    EnsemblBacteriaiBAD70070; BAD70070; BAD70070.
    GeneIDi3168343.
    KEGGittj:TTHA0247.
    PATRICi23955441. VBITheThe93045_0247.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AP008226 Genomic DNA. Translation: BAD70070.1 .
    L10348 Genomic DNA. No translation available.
    RefSeqi YP_143513.1. NC_006461.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1GIY X-ray 5.50 L 9-116 [» ]
    1YL3 X-ray 5.50 L 6-116 [» ]
    2B9P X-ray 6.46 K 1-141 [» ]
    2HGJ X-ray 5.00 L 1-147 [» ]
    2HGQ X-ray 5.50 L 1-147 [» ]
    2HGU X-ray 4.51 L 1-147 [» ]
    2J01 X-ray 2.80 K 1-147 [» ]
    2J03 X-ray 2.80 K 1-147 [» ]
    2WH2 X-ray 3.45 K 1-147 [» ]
    2WH4 X-ray 3.45 K 1-147 [» ]
    2WRJ X-ray 3.60 K 1-147 [» ]
    2WRL X-ray 3.60 K 1-147 [» ]
    2X9S X-ray 3.10 K 1-147 [» ]
    2X9U X-ray 3.10 K 1-147 [» ]
    2XTG electron microscopy 7.80 K 1-147 [» ]
    2XUX electron microscopy 7.60 K 1-147 [» ]
    3EGV X-ray 1.75 B 2-147 [» ]
    3FIN electron microscopy 6.40 L 2-139 [» ]
    3I8I X-ray 3.10 L 1-147 [» ]
    4JUX X-ray 2.86 K 1-147 [» ]
    ProteinModelPortali Q5SLP6.
    SMRi Q5SLP6. Positions 1-147.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q5SLP6. 1 interaction.
    MINTi MINT-4299414.
    STRINGi 300852.TTHA0247.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAD70070 ; BAD70070 ; BAD70070 .
    GeneIDi 3168343.
    KEGGi ttj:TTHA0247.
    PATRICi 23955441. VBITheThe93045_0247.

    Phylogenomic databases

    eggNOGi COG0080.
    HOGENOMi HOG000082123.
    KOi K02867.
    OMAi IEAYIKL.
    OrthoDBi EOG69PQ9D.
    PhylomeDBi Q5SLP6.

    Enzyme and pathway databases

    BioCyci TTHE300852:GH8R-257-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei Q5SLP6.

    Family and domain databases

    Gene3Di 1.10.10.250. 1 hit.
    3.30.1550.10. 1 hit.
    HAMAPi MF_00736. Ribosomal_L11.
    InterProi IPR000911. Ribosomal_L11/L12.
    IPR006519. Ribosomal_L11_bac-typ.
    IPR020783. Ribosomal_L11_C.
    IPR020785. Ribosomal_L11_CS.
    IPR020784. Ribosomal_L11_N.
    [Graphical view ]
    PANTHERi PTHR11661. PTHR11661. 1 hit.
    Pfami PF00298. Ribosomal_L11. 1 hit.
    PF03946. Ribosomal_L11_N. 1 hit.
    [Graphical view ]
    SMARTi SM00649. RL11. 1 hit.
    [Graphical view ]
    SUPFAMi SSF46906. SSF46906. 1 hit.
    SSF54747. SSF54747. 1 hit.
    TIGRFAMsi TIGR01632. L11_bact. 1 hit.
    PROSITEi PS00359. RIBOSOMAL_L11. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Complete genome sequence of Thermus thermophilus HB8."
      Masui R., Kurokawa K., Nakagawa N., Tokunaga F., Koyama Y., Shibata T., Oshima T., Yokoyama S., Yasunaga T., Kuramitsu S.
      Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: HB8 / ATCC 27634 / DSM 579.
    2. "Identification of the gene encoding transcription factor NusG of Thermus thermophilus."
      Heinrich T., Schroeder W., Erdmann V.A., Hartmann R.K.
      J. Bacteriol. 174:7859-7863(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-40.
      Strain: HB8 / ATCC 27634 / DSM 579.
    3. "Identification of the 50S ribosomal proteins from the eubacterium Thermus thermophilus."
      Katsani K.R., Tsiboli P., Anagnostopoulos K., Urlaub H., Choli-Papadopoulou T.
      Biol. Chem. 381:1079-1087(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 19-32 AND 71-85, BLOCKAGE OF N-TERMINUS.
    4. "Structural and functional studies on the overproduced L11 protein from Thermus thermophilus."
      Triantafillidou D., Simitsopoulou M., Franceschi F., Choli-Papadopoulou T.
      J. Protein Chem. 18:215-223(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: METHYLATION AT LYS-3.
    5. "Thermus thermophilus L11 methyltransferase, PrmA, is dispensable for growth and preferentially modifies free ribosomal protein L11 prior to ribosome assembly."
      Cameron D.M., Gregory S.T., Thompson J., Suh M.-J., Limbach P.A., Dahlberg A.E.
      J. Bacteriol. 186:5819-5825(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: MASS SPECTROMETRY, METHYLATION.
    6. "Extending ribosomal protein identifications to unsequenced bacterial strains using matrix-assisted laser desorption/ionization mass spectrometry."
      Suh M.-J., Hamburg D.M., Gregory S.T., Dahlberg A.E., Limbach P.A.
      Proteomics 5:4818-4831(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: MASS SPECTROMETRY.
    7. "The path of messenger RNA through the ribosome."
      Yusupova G.Z., Yusupov M.M., Cate J.H.D., Noller H.F.
      Cell 106:233-241(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (5.0 ANGSTROMS) OF THE RIBOSOME.
    8. Cited for: X-RAY CRYSTALLOGRAPHY (5.5 ANGSTROMS) OF THE RIBOSOME.

    Entry informationi

    Entry nameiRL11_THET8
    AccessioniPrimary (citable) accession number: Q5SLP6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 29, 2005
    Last sequence update: December 21, 2004
    Last modified: October 1, 2014
    This is version 83 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. Ribosomal proteins
      Ribosomal proteins families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3