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Q5SLP6 (RL11_THET8) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 82. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
50S ribosomal protein L11
Gene names
Name:rplK
Ordered Locus Names:TTHA0247
OrganismThermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) [Reference proteome] [HAMAP]
Taxonomic identifier300852 [NCBI]
Taxonomic lineageBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus

Protein attributes

Sequence length147 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Forms part of the ribosomal stalk which helps the ribosome interact with GTP-bound translation factors. The stalk extends beyond the surface of the 70S ribosome and is preferentially stabilized in 70S versus 50S crystals. HAMAP-Rule MF_00736

In the 70S ribosome is in a position where it could interact transiently with the A site tRNA during translation. HAMAP-Rule MF_00736

Subunit structure

Part of the ribosomal stalk of the 50S ribosomal subunit. Interacts with L10 and the large rRNA to form the base of the stalk. L10 forms an elongated spine to which 3 L12 dimers bind in a sequential fashion forming a heptameric L10(L12)2(L12)2(L12)2 complex. Contacts one of the L7 dimers.

Post-translational modification

The protein probably contains twelve methyl groups; at least one lysine residue (Lys-3) is known to be trimethylated. Methylation of the protein is not required for function. Ref.4 Ref.5

Sequence similarities

Belongs to the ribosomal protein L11P family.

Mass spectrometry

Molecular mass is 15506.2 Da from positions 1 - 147. Determined by MALDI. Weight of the unmethylated protein. Ref.5

Molecular mass is 15675.1 Da from positions 1 - 147. Determined by MALDI. Weight of the methylated protein. Ref.5

Molecular mass is 15678 Da from positions 1 - 147. Determined by MALDI. Ref.6

Ontologies

Keywords
   LigandRNA-binding
rRNA-binding
   Molecular functionRibonucleoprotein
Ribosomal protein
   PTMMethylation
   Technical term3D-structure
Complete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processtranslation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentribosome

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionLSU rRNA binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

structural constituent of ribosome

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 14714750S ribosomal protein L11 HAMAP-Rule MF_00736
PRO_0000104399

Amino acid modifications

Modified residue31N6,N6,N6-trimethyllysine Ref.4

Experimental info

Sequence conflict201A → G AA sequence Ref.3
Sequence conflict231V → G in L10348. Ref.2

Secondary structure

............................. 147
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q5SLP6 [UniParc].

Last modified December 21, 2004. Version 1.
Checksum: 3D8DECEBE85B5FE9

FASTA14715,505
        10         20         30         40         50         60 
MKKVVAVVKL QLPAGKATPA PPVGPALGQH GANIMEFVKA FNAATANMGD AIVPVEITIY 

        70         80         90        100        110        120 
ADRSFTFVTK TPPASYLIRK AAGLEKGAHK PGREKVGRIT WEQVLEIAKQ KMPDLNTTDL 

       130        140 
EAAARMIAGS ARSMGVEVVG APEVKDA 

« Hide

References

« Hide 'large scale' references
[1]"Complete genome sequence of Thermus thermophilus HB8."
Masui R., Kurokawa K., Nakagawa N., Tokunaga F., Koyama Y., Shibata T., Oshima T., Yokoyama S., Yasunaga T., Kuramitsu S.
Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: HB8 / ATCC 27634 / DSM 579.
[2]"Identification of the gene encoding transcription factor NusG of Thermus thermophilus."
Heinrich T., Schroeder W., Erdmann V.A., Hartmann R.K.
J. Bacteriol. 174:7859-7863(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-40.
Strain: HB8 / ATCC 27634 / DSM 579.
[3]"Identification of the 50S ribosomal proteins from the eubacterium Thermus thermophilus."
Katsani K.R., Tsiboli P., Anagnostopoulos K., Urlaub H., Choli-Papadopoulou T.
Biol. Chem. 381:1079-1087(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 19-32 AND 71-85, BLOCKAGE OF N-TERMINUS.
[4]"Structural and functional studies on the overproduced L11 protein from Thermus thermophilus."
Triantafillidou D., Simitsopoulou M., Franceschi F., Choli-Papadopoulou T.
J. Protein Chem. 18:215-223(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: METHYLATION AT LYS-3.
[5]"Thermus thermophilus L11 methyltransferase, PrmA, is dispensable for growth and preferentially modifies free ribosomal protein L11 prior to ribosome assembly."
Cameron D.M., Gregory S.T., Thompson J., Suh M.-J., Limbach P.A., Dahlberg A.E.
J. Bacteriol. 186:5819-5825(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: MASS SPECTROMETRY, METHYLATION.
[6]"Extending ribosomal protein identifications to unsequenced bacterial strains using matrix-assisted laser desorption/ionization mass spectrometry."
Suh M.-J., Hamburg D.M., Gregory S.T., Dahlberg A.E., Limbach P.A.
Proteomics 5:4818-4831(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: MASS SPECTROMETRY.
[7]"The path of messenger RNA through the ribosome."
Yusupova G.Z., Yusupov M.M., Cate J.H.D., Noller H.F.
Cell 106:233-241(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (5.0 ANGSTROMS) OF THE RIBOSOME.
[8]"Crystal structure of the ribosome at 5.5 A resolution."
Yusupov M.M., Yusupova G.Z., Baucom A., Lieberman K., Earnest T.N., Cate J.H.D., Noller H.F.
Science 292:883-896(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (5.5 ANGSTROMS) OF THE RIBOSOME.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AP008226 Genomic DNA. Translation: BAD70070.1.
L10348 Genomic DNA. No translation available.
RefSeqYP_143513.1. NC_006461.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1GIYX-ray5.50L9-116[»]
1YL3X-ray5.50L6-116[»]
2B9PX-ray6.46K1-141[»]
2HGJX-ray5.00L1-147[»]
2HGQX-ray5.50L1-147[»]
2HGUX-ray4.51L1-147[»]
2J01X-ray2.80K1-147[»]
2J03X-ray2.80K1-147[»]
2WH2X-ray3.45K1-147[»]
2WH4X-ray3.45K1-147[»]
2WRJX-ray3.60K1-147[»]
2WRLX-ray3.60K1-147[»]
2X9SX-ray3.10K1-147[»]
2X9UX-ray3.10K1-147[»]
2XTGelectron microscopy7.80K1-147[»]
2XUXelectron microscopy7.60K1-147[»]
3EGVX-ray1.75B2-147[»]
3FINelectron microscopy6.40L2-139[»]
3I8IX-ray3.10L1-147[»]
4JUXX-ray2.86K1-147[»]
ProteinModelPortalQ5SLP6.
SMRQ5SLP6. Positions 1-147.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ5SLP6. 1 interaction.
MINTMINT-4299414.
STRING300852.TTHA0247.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAD70070; BAD70070; BAD70070.
GeneID3168343.
KEGGttj:TTHA0247.
PATRIC23955441. VBITheThe93045_0247.

Phylogenomic databases

eggNOGCOG0080.
HOGENOMHOG000082123.
KOK02867.
OMAIEAYIKL.
OrthoDBEOG69PQ9D.
PhylomeDBQ5SLP6.

Enzyme and pathway databases

BioCycTTHE300852:GH8R-257-MONOMER.

Family and domain databases

Gene3D1.10.10.250. 1 hit.
3.30.1550.10. 1 hit.
HAMAPMF_00736. Ribosomal_L11.
InterProIPR000911. Ribosomal_L11/L12.
IPR006519. Ribosomal_L11_bac-typ.
IPR020783. Ribosomal_L11_C.
IPR020785. Ribosomal_L11_CS.
IPR020784. Ribosomal_L11_N.
[Graphical view]
PANTHERPTHR11661. PTHR11661. 1 hit.
PfamPF00298. Ribosomal_L11. 1 hit.
PF03946. Ribosomal_L11_N. 1 hit.
[Graphical view]
SMARTSM00649. RL11. 1 hit.
[Graphical view]
SUPFAMSSF46906. SSF46906. 1 hit.
SSF54747. SSF54747. 1 hit.
TIGRFAMsTIGR01632. L11_bact. 1 hit.
PROSITEPS00359. RIBOSOMAL_L11. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceQ5SLP6.

Entry information

Entry nameRL11_THET8
AccessionPrimary (citable) accession number: Q5SLP6
Entry history
Integrated into UniProtKB/Swiss-Prot: March 29, 2005
Last sequence update: December 21, 2004
Last modified: July 9, 2014
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Ribosomal proteins

Ribosomal proteins families and list of entries

PDB cross-references

Index of Protein Data Bank (PDB) cross-references