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Protein

Thermostable carboxypeptidase 1

Gene

TTHA0270

Organism
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Broad specificity carboxypetidase that releases amino acids sequentially from the C-terminus, including neutral, aromatic, polar and basic residues, but not Pro. Has lower activity with substrates ending with Gly or Glu.1 Publication

Catalytic activityi

Release of a C-terminal amino acid with broad specificity, except for -Pro.1 Publication

Cofactori

Zn2+1 PublicationNote: Binds 1 zinc ion per subunit.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi276Zinc; via tele nitrogen1 Publication1
Active sitei277Proton donor/acceptorBy similarity1
Metal bindingi280Zinc; via tele nitrogen1 Publication1
Metal bindingi306Zinc1 Publication1

GO - Molecular functioni

  • metallocarboxypeptidase activity Source: UniProtKB
  • zinc ion binding Source: UniProtKB

GO - Biological processi

  • proteolysis Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Carboxypeptidase, Hydrolase, Metalloprotease, Protease

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciTTHE300852:GH8R-283-MONOMER.

Protein family/group databases

MEROPSiM32.001.

Names & Taxonomyi

Protein namesi
Recommended name:
Thermostable carboxypeptidase 1 (EC:3.4.17.19)
Alternative name(s):
TthCP1
Gene namesi
Ordered Locus Names:TTHA0270
OrganismiThermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Taxonomic identifieri300852 [NCBI]
Taxonomic lineageiBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus
Proteomesi
  • UP000000532 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004288351 – 510Thermostable carboxypeptidase 1Add BLAST510

Interactioni

Subunit structurei

Homodimer.2 Publications

Protein-protein interaction databases

STRINGi300852.TTHA0270.

Structurei

Secondary structure

1510
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi3 – 32Combined sources30
Helixi40 – 59Combined sources20
Helixi61 – 70Combined sources10
Helixi74 – 76Combined sources3
Helixi82 – 99Combined sources18
Helixi102 – 122Combined sources21
Turni123 – 126Combined sources4
Helixi128 – 150Combined sources23
Helixi166 – 172Combined sources7
Helixi178 – 200Combined sources23
Helixi208 – 212Combined sources5
Helixi217 – 231Combined sources15
Helixi235 – 237Combined sources3
Beta strandi238 – 242Combined sources5
Beta strandi248 – 252Combined sources5
Beta strandi255 – 260Combined sources6
Helixi268 – 286Combined sources19
Helixi289 – 291Combined sources3
Helixi295 – 297Combined sources3
Helixi302 – 313Combined sources12
Turni314 – 318Combined sources5
Helixi320 – 333Combined sources14
Helixi335 – 337Combined sources3
Helixi342 – 348Combined sources7
Helixi358 – 360Combined sources3
Turni363 – 365Combined sources3
Helixi366 – 381Combined sources16
Helixi390 – 402Combined sources13
Beta strandi407 – 409Combined sources3
Turni410 – 414Combined sources5
Turni418 – 422Combined sources5
Helixi428 – 448Combined sources21
Helixi452 – 456Combined sources5
Helixi461 – 470Combined sources10
Helixi472 – 474Combined sources3
Helixi480 – 488Combined sources9
Helixi495 – 508Combined sources14

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1WGZX-ray2.60A/B/C1-510[»]
3HOAX-ray2.10A/B1-509[»]
ProteinModelPortaliQ5SLM3.
SMRiQ5SLM3.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ5SLM3.

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase M32 family.Curated

Phylogenomic databases

eggNOGiENOG4105CTD. Bacteria.
COG2317. LUCA.
HOGENOMiHOG000040803.
KOiK01299.
OMAiEFGHALY.
PhylomeDBiQ5SLM3.

Family and domain databases

CDDicd06460. M32_Taq. 1 hit.
InterProiIPR001333. Peptidase_M32_Taq.
[Graphical view]
PfamiPF02074. Peptidase_M32. 1 hit.
[Graphical view]
PIRSFiPIRSF006615. Zn_crbxpep_Taq. 1 hit.
PRINTSiPR00998. CRBOXYPTASET.

Sequencei

Sequence statusi: Complete.

Q5SLM3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTPEAAYQNL LEFQRETAYL ASLGALAAWD QRTMIPKKGH EHRARQMAAL
60 70 80 90 100
ARLLHQRMTD PRIGEWLEKV EGSPLVQDPL SDAAVNVREW RQAYERARAI
110 120 130 140 150
PERLAVELAQ AESEAESFWE EARPRDDWRG FLPYLKRVYA LTKEKAEVLF
160 170 180 190 200
ALPPAPGDPP YGELYDALLD GYEPGMRARE LLPLFAELKE GLKGLLDRIL
210 220 230 240 250
GSGKRPDTSI LHRPYPVEAQ RRFALELLSA CGYDLEAGRL DPTAHPFEIA
260 270 280 290 300
IGPGDVRITT RYYEDFFNAG IFGTLHEMGH ALYEQGLPKE HWGTPRGDAV
310 320 330 340 350
SLGVHESQSR TWENLVGRSL GFWERFFPRA REVFASLGDV SLEDFHFAVN
360 370 380 390 400
AVEPSLIRVE ADEVTYNLHI LVRLELELAL FRGELSPEDL PEAWAEKYRD
410 420 430 440 450
HLGVAPKDYK DGVMQDVHWA GGLFGYFPTY TLGNLYAAQF FQKAEAELGP
460 470 480 490 500
LEPRFARGEF QPFLDWTRAR IHAEGSRFRP RVLVERVTGE APSARPFLAY
510
LEKKYAALYG
Length:510
Mass (Da):58,014
Last modified:December 21, 2004 - v1
Checksum:i6EE48D42AA2FA379
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP008226 Genomic DNA. Translation: BAD70093.1.
RefSeqiWP_011174078.1. NC_006461.1.
YP_143536.1. NC_006461.1.

Genome annotation databases

EnsemblBacteriaiBAD70093; BAD70093; BAD70093.
GeneIDi3168368.
KEGGittj:TTHA0270.
PATRICi23955495. VBITheThe93045_0270.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP008226 Genomic DNA. Translation: BAD70093.1.
RefSeqiWP_011174078.1. NC_006461.1.
YP_143536.1. NC_006461.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1WGZX-ray2.60A/B/C1-510[»]
3HOAX-ray2.10A/B1-509[»]
ProteinModelPortaliQ5SLM3.
SMRiQ5SLM3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi300852.TTHA0270.

Protein family/group databases

MEROPSiM32.001.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAD70093; BAD70093; BAD70093.
GeneIDi3168368.
KEGGittj:TTHA0270.
PATRICi23955495. VBITheThe93045_0270.

Phylogenomic databases

eggNOGiENOG4105CTD. Bacteria.
COG2317. LUCA.
HOGENOMiHOG000040803.
KOiK01299.
OMAiEFGHALY.
PhylomeDBiQ5SLM3.

Enzyme and pathway databases

BioCyciTTHE300852:GH8R-283-MONOMER.

Miscellaneous databases

EvolutionaryTraceiQ5SLM3.

Family and domain databases

CDDicd06460. M32_Taq. 1 hit.
InterProiIPR001333. Peptidase_M32_Taq.
[Graphical view]
PfamiPF02074. Peptidase_M32. 1 hit.
[Graphical view]
PIRSFiPIRSF006615. Zn_crbxpep_Taq. 1 hit.
PRINTSiPR00998. CRBOXYPTASET.
ProtoNetiSearch...

Entry informationi

Entry nameiCBP1_THET8
AccessioniPrimary (citable) accession number: Q5SLM3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 16, 2014
Last sequence update: December 21, 2004
Last modified: November 2, 2016
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.