Holliday junction ATP-dependent DNA helicase RuvB - Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)

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Q5SL87 (RUVB_THET8) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 55. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Holliday junction ATP-dependent DNA helicase RuvB
EC=3.6.4.12

Gene names

Name:	ruvB
Ordered Locus Names:	TTHA0406

Organism

Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)

Taxonomic identifier

300852 [NCBI]

Taxonomic lineage

Bacteria › Deinococcus-Thermus › Deinococci › Thermales › Thermaceae › Thermus

Protein attributes

Sequence length	324 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	The RuvA-RuvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is an helicase that mediates the Holliday junction migration by localized denaturation and reannealing. RuvB is a Mg²⁺-dependent, DNA-dependent ATPase with an equal preference for supercoiled and linear duplex DNA. It can promote Holliday junction migration alone. Ref.1
Catalytic activity	ATP + H₂O = ADP + phosphate. HAMAP MF_00016
Enzyme regulation	The activity of RuvB is enhanced by RuvA. HAMAP MF_00016
Subunit structure	Forms a complex with RuvA.
Sequence similarities	Belongs to the RuvB family.

Ontologies

Keywords
Biological process	DNA damage DNA recombination DNA repair SOS response
Ligand	ATP-binding Nucleotide-binding
Molecular function	Helicase Hydrolase
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	DNA recombination Inferred from electronic annotation. Source: UniProtKB-KW DNA repair Inferred from electronic annotation. Source: UniProtKB-KW SOS response Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA binding Inferred from electronic annotation. Source: InterPro four-way junction helicase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 324

324

Holliday junction ATP-dependent DNA helicase RuvB HAMAP MF_00016

PRO_0000165619

Regions

Nucleotide binding

45 – 52

ATP Potential

Secondary structure

324

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

Q5SL87 [UniParc].

Last modified December 21, 2004. Version 1.
Checksum: F23C50F71F14D7DB
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FASTA

324

35,974

        10         20         30         40         50         60 
MEDLALRPKT LDEYIGQERL KQKLRVYLEA AKARKEPLEH LLLFGPPGLG KTTLAHVIAH 

        70         80         90        100        110        120 
ELGVNLRVTS GPAIEKPGDL AAILANSLEE GDILFIDEIH RLSRQAEEHL YPAMEDFVMD 

       130        140        150        160        170        180 
IVIGQGPAAR TIRLELPRFT LIGATTRPGL ITAPLLSRFG IVEHLEYYTP EELAQGVMRD 

       190        200        210        220        230        240 
ARLLGVRITE EAALEIGRRS RGTMRVAKRL FRRVRDFAQV AGEEVITRER ALEALAALGL 

       250        260        270        280        290        300 
DELGLEKRDR EILEVLILRF GGGPVGLATL ATALSEDPGT LEEVHEPYLI RQGLLKRTPR 

       310        320 
GRVATELAYR HLGYPPPVGP LLEP

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Novel properties of the Thermus thermophilus RuvB protein, which promotes branch migration of Holliday junctions." Yamada K., Fukuoh A., Iwasaki H., Shinagawa H. Mol. Gen. Genet. 261:1001-1011(1999) [PubMed: 10485292] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
[2]	"Complete genome sequence of Thermus thermophilus HB8." Masui R., Kurokawa K., Nakagawa N., Tokunaga F., Koyama Y., Shibata T., Oshima T., Yokoyama S., Yasunaga T., Kuramitsu S. Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: HB8 / ATCC 27634 / DSM 579.
[3]	"Crystal structure of the Holliday junction migration motor protein RuvB from Thermus thermophilus HB8." Yamada K., Kunishima N., Mayanagi K., Ohnishi T., Nishino T., Iwasaki H., Shinagawa H., Morikawa K. Proc. Natl. Acad. Sci. U.S.A. 98:1442-1447(2001) [PubMed: 11171970] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (3.2 ANGSTROMS).
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AB015236 Genomic DNA. Translation: BAA76480.2.
AP008226 Genomic DNA. Translation: BAD70229.1.

RefSeq

YP_143672.1. NC_006461.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1HQC	X-ray	3.20	A/B	1-324	[»]
1IXR	X-ray	3.30	C	1-312	[»]
1IXS	X-ray	3.20	B	1-318	[»]

ProteinModelPortal

Q5SL87.

SMR

Q5SL87. Positions 4-318.

ModBase

Search...

Protein-protein interaction databases

IntAct

Q5SL87. 1 interaction.

STRING

Q5SL87.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

GeneID

3168960.

GenomeReviews

Gene locus TTHA0406 in contig AP008226_GR.

KEGG

ttj:TTHA0406.

NMPDR

fig|300852.3.peg.435.

PATRIC

23955783. VBITheThe93045_0406.

Phylogenomic databases

eggNOG

COG2255.

HOGENOM

HBG339960.

OMA

EKFDGGP.

PhylomeDB

Q5SL87.

ProtClustDB

PRK00080.

Enzyme and pathway databases

BioCyc

TTHE300852:TTHA0406-MONOMER.

Family and domain databases

HAMAP

MF_00016. DNA_helic_RuvB.
[Tree]

InterPro

IPR003593. ATPase_AAA+_core.
IPR004605. DNA_helicase_Holl-junc_RuvB.
IPR008823. DNA_helicase_Holl-junc_RuvB_C.
IPR008824. DNA_helicase_Holl-junc_RuvB_N.
IPR011991. WHTH_trsnscrt_rep_DNA-bd.
[Graphical view]

Gene3D

G3DSA:1.10.10.10. Wing_hlx_DNA_bd. 1 hit.

K03551.

PANTHER

PTHR13779:SF2. PTHR13779:SF2. 1 hit.

Pfam

PF05491. RuvB_C. 1 hit.
PF05496. RuvB_N. 1 hit.
[Graphical view]

ProDom

PD005323. DNA_helicase_Holl-junc_RuvB_C. 1 hit.
[Graphical view] [Entries sharing at least one domain]

SMART

SM00382. AAA. 1 hit.
[Graphical view]

TIGRFAMs

TIGR00635. RuvB. 1 hit.

ProtoNet

Search...

Other

DrugBank

DB00173. Adenine.

Entry information

Entry name

RUVB_THET8

Accession

Primary (citable) accession number: Q5SL87

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 29, 2005
Last sequence update:	December 21, 2004
Last modified:	January 25, 2012
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protocols and materials databases

Genome annotation databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

Other

Entry information

Relevant documents