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Protein

Holliday junction ATP-dependent DNA helicase RuvB

Gene

ruvB

Organism
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

The RuvA-RuvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is a helicase that mediates the Holliday junction migration by localized denaturation and reannealing. RuvB is a Mg2+-dependent, DNA-dependent ATPase with an equal preference for supercoiled and linear duplex DNA. It can promote Holliday junction migration alone.UniRule annotation1 Publication

Catalytic activityi

ATP + H2O = ADP + phosphate.UniRule annotation1 Publication

Enzyme regulationi

The activity of RuvB is enhanced by RuvA.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi45 – 528ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Biological processi

DNA damage, DNA recombination, DNA repair, SOS response

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciTTHE300852:GH8R-427-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Holliday junction ATP-dependent DNA helicase RuvBUniRule annotation (EC:3.6.4.12UniRule annotation)
Gene namesi
Name:ruvBUniRule annotation
Ordered Locus Names:TTHA0406
OrganismiThermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Taxonomic identifieri300852 [NCBI]
Taxonomic lineageiBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus
Proteomesi
  • UP000000532 Componenti: Chromosome

Pathology & Biotechi

Chemistry

DrugBankiDB00173. Adenine.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 324324Holliday junction ATP-dependent DNA helicase RuvBPRO_0000165619Add
BLAST

Interactioni

Subunit structurei

Forms a complex with RuvA.UniRule annotation2 Publications

Protein-protein interaction databases

IntActiQ5SL87. 1 interaction.
STRINGi300852.TTHA0406.

Structurei

Secondary structure

1
324
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Turni11 – 133Combined sources
Helixi18 – 3417Combined sources
Beta strandi42 – 443Combined sources
Beta strandi45 – 517Combined sources
Helixi52 – 6211Combined sources
Beta strandi66 – 694Combined sources
Turni71 – 733Combined sources
Helixi77 – 848Combined sources
Turni85 – 873Combined sources
Beta strandi93 – 964Combined sources
Turni97 – 1004Combined sources
Helixi104 – 11613Combined sources
Beta strandi117 – 1215Combined sources
Beta strandi124 – 1285Combined sources
Beta strandi132 – 1354Combined sources
Beta strandi140 – 1467Combined sources
Turni149 – 1513Combined sources
Turni155 – 1584Combined sources
Beta strandi162 – 1643Combined sources
Helixi170 – 18213Combined sources
Turni183 – 1853Combined sources
Helixi190 – 19910Combined sources
Helixi204 – 21411Combined sources
Turni215 – 2173Combined sources
Turni219 – 2213Combined sources
Helixi228 – 23811Combined sources
Beta strandi241 – 2444Combined sources
Helixi247 – 25913Combined sources
Helixi267 – 2737Combined sources
Helixi278 – 2847Combined sources
Helixi286 – 2916Combined sources
Beta strandi294 – 2985Combined sources
Beta strandi301 – 3044Combined sources
Helixi306 – 3116Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1HQCX-ray3.20A/B1-324[»]
1IXRX-ray3.30C1-312[»]
1IXSX-ray3.20B1-318[»]
ProteinModelPortaliQ5SL87.
SMRiQ5SL87. Positions 4-318.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ5SL87.

Family & Domainsi

Sequence similaritiesi

Belongs to the RuvB family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CKJ. Bacteria.
COG2255. LUCA.
HOGENOMiHOG000218623.
KOiK03551.
OMAiYEPYLMQ.
PhylomeDBiQ5SL87.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
3.40.50.300. 1 hit.
HAMAPiMF_00016. DNA_helic_RuvB. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR004605. DNA_helicase_Holl-junc_RuvB.
IPR008823. DNA_helicase_Holl-junc_RuvB_C.
IPR027417. P-loop_NTPase.
IPR008824. RuvB_N.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF05491. RuvB_C. 1 hit.
PF05496. RuvB_N. 1 hit.
[Graphical view]
ProDomiPD005323. DNA_helicase_Holl-junc_RuvB_C. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00635. ruvB. 1 hit.

Sequencei

Sequence statusi: Complete.

Q5SL87-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEDLALRPKT LDEYIGQERL KQKLRVYLEA AKARKEPLEH LLLFGPPGLG
60 70 80 90 100
KTTLAHVIAH ELGVNLRVTS GPAIEKPGDL AAILANSLEE GDILFIDEIH
110 120 130 140 150
RLSRQAEEHL YPAMEDFVMD IVIGQGPAAR TIRLELPRFT LIGATTRPGL
160 170 180 190 200
ITAPLLSRFG IVEHLEYYTP EELAQGVMRD ARLLGVRITE EAALEIGRRS
210 220 230 240 250
RGTMRVAKRL FRRVRDFAQV AGEEVITRER ALEALAALGL DELGLEKRDR
260 270 280 290 300
EILEVLILRF GGGPVGLATL ATALSEDPGT LEEVHEPYLI RQGLLKRTPR
310 320
GRVATELAYR HLGYPPPVGP LLEP
Length:324
Mass (Da):35,974
Last modified:December 21, 2004 - v1
Checksum:iF23C50F71F14D7DB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB015236 Genomic DNA. Translation: BAA76480.2.
AP008226 Genomic DNA. Translation: BAD70229.1.
RefSeqiWP_011227913.1. NC_006461.1.
YP_143672.1. NC_006461.1.

Genome annotation databases

EnsemblBacteriaiBAD70229; BAD70229; BAD70229.
GeneIDi3168960.
KEGGittj:TTHA0406.
PATRICi23955783. VBITheThe93045_0406.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB015236 Genomic DNA. Translation: BAA76480.2.
AP008226 Genomic DNA. Translation: BAD70229.1.
RefSeqiWP_011227913.1. NC_006461.1.
YP_143672.1. NC_006461.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1HQCX-ray3.20A/B1-324[»]
1IXRX-ray3.30C1-312[»]
1IXSX-ray3.20B1-318[»]
ProteinModelPortaliQ5SL87.
SMRiQ5SL87. Positions 4-318.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ5SL87. 1 interaction.
STRINGi300852.TTHA0406.

Chemistry

DrugBankiDB00173. Adenine.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAD70229; BAD70229; BAD70229.
GeneIDi3168960.
KEGGittj:TTHA0406.
PATRICi23955783. VBITheThe93045_0406.

Phylogenomic databases

eggNOGiENOG4105CKJ. Bacteria.
COG2255. LUCA.
HOGENOMiHOG000218623.
KOiK03551.
OMAiYEPYLMQ.
PhylomeDBiQ5SL87.

Enzyme and pathway databases

BioCyciTTHE300852:GH8R-427-MONOMER.

Miscellaneous databases

EvolutionaryTraceiQ5SL87.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
3.40.50.300. 1 hit.
HAMAPiMF_00016. DNA_helic_RuvB. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR004605. DNA_helicase_Holl-junc_RuvB.
IPR008823. DNA_helicase_Holl-junc_RuvB_C.
IPR027417. P-loop_NTPase.
IPR008824. RuvB_N.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF05491. RuvB_C. 1 hit.
PF05496. RuvB_N. 1 hit.
[Graphical view]
ProDomiPD005323. DNA_helicase_Holl-junc_RuvB_C. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00635. ruvB. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiRUVB_THET8
AccessioniPrimary (citable) accession number: Q5SL87
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 29, 2005
Last sequence update: December 21, 2004
Last modified: September 7, 2016
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.