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Protein

Holliday junction ATP-dependent DNA helicase RuvB

Gene

ruvB

Organism
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

The RuvA-RuvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is a helicase that mediates the Holliday junction migration by localized denaturation and reannealing. RuvB is a Mg2+-dependent, DNA-dependent ATPase with an equal preference for supercoiled and linear duplex DNA. It can promote Holliday junction migration alone.UniRule annotation1 Publication

Catalytic activityi

ATP + H2O = ADP + phosphate.UniRule annotation1 Publication

Enzyme regulationi

The activity of RuvB is enhanced by RuvA.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi45 – 52ATPUniRule annotation8

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Biological processi

DNA damage, DNA recombination, DNA repair, SOS response

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Holliday junction ATP-dependent DNA helicase RuvBUniRule annotation (EC:3.6.4.12UniRule annotation)
Gene namesi
Name:ruvBUniRule annotation
Ordered Locus Names:TTHA0406
OrganismiThermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Taxonomic identifieri300852 [NCBI]
Taxonomic lineageiBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus
Proteomesi
  • UP000000532 Componenti: Chromosome

Pathology & Biotechi

Chemistry databases

DrugBankiDB00173. Adenine.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001656191 – 324Holliday junction ATP-dependent DNA helicase RuvBAdd BLAST324

Interactioni

Subunit structurei

Forms a complex with RuvA.UniRule annotation2 Publications

Protein-protein interaction databases

IntActiQ5SL87. 1 interactor.
STRINGi300852.TTHA0406.

Structurei

Secondary structure

1324
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni11 – 13Combined sources3
Helixi18 – 34Combined sources17
Beta strandi42 – 44Combined sources3
Beta strandi45 – 51Combined sources7
Helixi52 – 62Combined sources11
Beta strandi66 – 69Combined sources4
Turni71 – 73Combined sources3
Helixi77 – 84Combined sources8
Turni85 – 87Combined sources3
Beta strandi93 – 96Combined sources4
Turni97 – 100Combined sources4
Helixi104 – 116Combined sources13
Beta strandi117 – 121Combined sources5
Beta strandi124 – 128Combined sources5
Beta strandi132 – 135Combined sources4
Beta strandi140 – 146Combined sources7
Turni149 – 151Combined sources3
Turni155 – 158Combined sources4
Beta strandi162 – 164Combined sources3
Helixi170 – 182Combined sources13
Turni183 – 185Combined sources3
Helixi190 – 199Combined sources10
Helixi204 – 214Combined sources11
Turni215 – 217Combined sources3
Turni219 – 221Combined sources3
Helixi228 – 238Combined sources11
Beta strandi241 – 244Combined sources4
Helixi247 – 259Combined sources13
Helixi267 – 273Combined sources7
Helixi278 – 284Combined sources7
Helixi286 – 291Combined sources6
Beta strandi294 – 298Combined sources5
Beta strandi301 – 304Combined sources4
Helixi306 – 311Combined sources6

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1HQCX-ray3.20A/B1-324[»]
1IXRX-ray3.30C1-312[»]
1IXSX-ray3.20B1-318[»]
ProteinModelPortaliQ5SL87.
SMRiQ5SL87.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ5SL87.

Family & Domainsi

Sequence similaritiesi

Belongs to the RuvB family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CKJ. Bacteria.
COG2255. LUCA.
HOGENOMiHOG000218623.
KOiK03551.
OMAiYEPYLMQ.
PhylomeDBiQ5SL87.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
3.40.50.300. 1 hit.
HAMAPiMF_00016. DNA_helic_RuvB. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR004605. DNA_helicase_Holl-junc_RuvB.
IPR008823. DNA_helicase_Holl-junc_RuvB_C.
IPR027417. P-loop_NTPase.
IPR008824. RuvB_N.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF05491. RuvB_C. 1 hit.
PF05496. RuvB_N. 1 hit.
[Graphical view]
ProDomiPD005323. DNA_helicase_Holl-junc_RuvB_C. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00635. ruvB. 1 hit.

Sequencei

Sequence statusi: Complete.

Q5SL87-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEDLALRPKT LDEYIGQERL KQKLRVYLEA AKARKEPLEH LLLFGPPGLG
60 70 80 90 100
KTTLAHVIAH ELGVNLRVTS GPAIEKPGDL AAILANSLEE GDILFIDEIH
110 120 130 140 150
RLSRQAEEHL YPAMEDFVMD IVIGQGPAAR TIRLELPRFT LIGATTRPGL
160 170 180 190 200
ITAPLLSRFG IVEHLEYYTP EELAQGVMRD ARLLGVRITE EAALEIGRRS
210 220 230 240 250
RGTMRVAKRL FRRVRDFAQV AGEEVITRER ALEALAALGL DELGLEKRDR
260 270 280 290 300
EILEVLILRF GGGPVGLATL ATALSEDPGT LEEVHEPYLI RQGLLKRTPR
310 320
GRVATELAYR HLGYPPPVGP LLEP
Length:324
Mass (Da):35,974
Last modified:December 21, 2004 - v1
Checksum:iF23C50F71F14D7DB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB015236 Genomic DNA. Translation: BAA76480.2.
AP008226 Genomic DNA. Translation: BAD70229.1.
RefSeqiWP_011227913.1. NC_006461.1.
YP_143672.1. NC_006461.1.

Genome annotation databases

EnsemblBacteriaiBAD70229; BAD70229; BAD70229.
GeneIDi3168960.
KEGGittj:TTHA0406.
PATRICi23955783. VBITheThe93045_0406.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB015236 Genomic DNA. Translation: BAA76480.2.
AP008226 Genomic DNA. Translation: BAD70229.1.
RefSeqiWP_011227913.1. NC_006461.1.
YP_143672.1. NC_006461.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1HQCX-ray3.20A/B1-324[»]
1IXRX-ray3.30C1-312[»]
1IXSX-ray3.20B1-318[»]
ProteinModelPortaliQ5SL87.
SMRiQ5SL87.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ5SL87. 1 interactor.
STRINGi300852.TTHA0406.

Chemistry databases

DrugBankiDB00173. Adenine.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAD70229; BAD70229; BAD70229.
GeneIDi3168960.
KEGGittj:TTHA0406.
PATRICi23955783. VBITheThe93045_0406.

Phylogenomic databases

eggNOGiENOG4105CKJ. Bacteria.
COG2255. LUCA.
HOGENOMiHOG000218623.
KOiK03551.
OMAiYEPYLMQ.
PhylomeDBiQ5SL87.

Miscellaneous databases

EvolutionaryTraceiQ5SL87.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
3.40.50.300. 1 hit.
HAMAPiMF_00016. DNA_helic_RuvB. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR004605. DNA_helicase_Holl-junc_RuvB.
IPR008823. DNA_helicase_Holl-junc_RuvB_C.
IPR027417. P-loop_NTPase.
IPR008824. RuvB_N.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF05491. RuvB_C. 1 hit.
PF05496. RuvB_N. 1 hit.
[Graphical view]
ProDomiPD005323. DNA_helicase_Holl-junc_RuvB_C. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00635. ruvB. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiRUVB_THET8
AccessioniPrimary (citable) accession number: Q5SL87
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 29, 2005
Last sequence update: December 21, 2004
Last modified: November 2, 2016
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.