Q5SKZ7 (NQO15_THET8) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NADH-quinone oxidoreductase subunit 15
EC=1.6.99.5

Alternative name(s):
NADH dehydrogenase I chain 15
NDH-1 subunit 15

Gene names

Name:	nqo15
Ordered Locus Names:	TTHA0496

Organism

Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) [Reference proteome] [HAMAP]

Taxonomic identifier

300852 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Deinococcus-Thermus › Deinococci › Thermales › Thermaceae › Thermus › Thermus thermophilus

Protein attributes

Sequence length	129 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is menaquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient required for the synthesis of ATP. The nqo15 subunit has probably a role in complex stabilization, and may be also involved in the storage of iron for iron-sulfur cluster regeneration in the complex. Ref.3
Catalytic activity	NADH + quinone = NAD+ + quinol.
Subunit structure	NDH-1 is composed of 15 different subunits, nqo1 to nqo15. The complex has a L-shaped structure, with the hydrophobic arm (subunits nqo7, nqo8 and nqo10 to nqo14) embedded in the membrane and the hydrophilic peripheral arm (subunits nqo1 to nqo6, nqo9 and nqo15) protruding into the bacterial cytoplasm. The hydrophilic domain contains all the redox centers. Nqo15 is bound to the side of the complex near the N-terminus of nqo3, where it interacts with subunits nqo3, nqo2, nqo1, nqo9 and nqo4. Ref.2 Ref.3
Subcellular location	Cell membrane; Peripheral membrane protein; Cytoplasmic side.
Domain	Has a similar fold to the mitochondrial iron chaperone frataxin.
Sequence similarities	Belongs to the complex I nqo15 family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Ligand	NAD
Molecular function	Oxidoreductase
PTM	Quinone
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	iron-sulfur cluster assembly Inferred from electronic annotation. Source: InterPro
Cellular_component	plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	NADH dehydrogenase (quinone) activity Inferred from electronic annotation. Source: EC ferric iron binding Inferred from electronic annotation. Source: InterPro quinone binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed Ref.2

Chain

2 – 129

128

NADH-quinone oxidoreductase subunit 15

PRO_0000233014

Secondary structure

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129

Helix Strand Turn

Details...

Helix

5 – 26

22

Beta strand

31 – 36

6

Helix

38 – 41

4

Beta strand

53 – 60

8

Beta strand

62 – 64

3

Beta strand

66 – 72

7

Beta strand

75 – 79

5

Beta strand

82 – 86

5

Turn

87 – 90

4

Beta strand

91 – 98

8

Turn

99 – 101

3

Beta strand

102 – 105

4

Helix

112 – 126

15

Sequences

	Sequence		Length	Mass (Da)
	Q5SKZ7 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: A944E174C0DE152C	FASTA	129	14,788
	10 20 30 40 50 60 MSASSERELY EAWVELLSWM REYAQAKGVR FEKEADFPDF IYRMERPYDL PTTIMTASLS 70 80 90 100 110 120 DGLGEPFLLA DVSPRHAKLK RIGLRLPRAH IHLHAHYEPG KGLVTGKIPL TKERFFALAD RAREALAFA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete genome sequence of Thermus thermophilus HB8." Masui R., Kurokawa K., Nakagawa N., Tokunaga F., Koyama Y., Shibata T., Oshima T., Yokoyama S., Yasunaga T., Kuramitsu S. Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: HB8 / ATCC 27634 / DSM 579.
[2]	"Identification of a novel subunit of respiratory complex I from Thermus thermophilus." Hinchliffe P., Carroll J., Sazanov L.A. Biochemistry 45:4413-4420(2006) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-9, CHARACTERIZATION, IDENTIFICATION BY MASS SPECTROMETRY, SUBUNIT.
[3]	"Structure of the hydrophilic domain of respiratory complex I from Thermus thermophilus." Sazanov L.A., Hinchliffe P. Science 311:1430-1436(2006) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (3.3 ANGSTROMS), FUNCTION, SUBUNIT, ELECTRON TRANSFER MECHANISM.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AP008226 Genomic DNA. Translation: BAD70319.1.

RefSeq

YP_143762.1. NC_006461.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2FUG	X-ray	3.30	7/H/Q/Z	1-129	[»]
2YBB	electron microscopy	19.00	7	1-129	[»]
3I9V	X-ray	3.10	7/H	1-129	[»]
3IAM	X-ray	3.10	7/H	1-129	[»]
3IAS	X-ray	3.15	7/H/Q/Z	1-129	[»]
3M9S	X-ray	4.50	7/J	1-129	[»]
4HEA	X-ray	3.30	7/I	1-129	[»]

ProteinModelPortal

Q5SKZ7.

SMR

Q5SKZ7. Positions 3-129.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-59273N.

STRING

300852.TTHA0496.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

BAD70319; BAD70319; BAD70319.

GeneID

3169322.

KEGG

ttj:TTHA0496.

PATRIC

23955967. VBITheThe93045_0494.

Phylogenomic databases

eggNOG

NOG43023.

HOGENOM

HOG000099234.

OMA

IYRMERP.

ProtClustDB

CLSK444677.

Family and domain databases

InterPro

IPR002908. Frataxin/CyaY.
IPR021093. NADH_quinone_OxRdtase_su15.
[Graphical view]

Pfam

PF11497. NADH_Oxid_Nqo15. 1 hit.
[Graphical view]

SUPFAM

SSF55387. Frataxin_like. 1 hit.

ProtoNet

Search...

Other

EvolutionaryTrace

Q5SKZ7.

Entry information

Entry name

NQO15_THET8

Accession

Primary (citable) accession number: Q5SKZ7

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 18, 2006
Last sequence update:	January 23, 2007
Last modified:	May 1, 2013
This is version 57 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program