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Protein

NADH-quinone oxidoreductase subunit 15

Gene

nqo15

Organism
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is menaquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient required for the synthesis of ATP. The Nqo15 subunit has probably a role in complex stabilization, and may be also involved in the storage of iron for iron-sulfur cluster regeneration in the complex.2 Publications

Catalytic activityi

NADH + a quinone = NAD+ + a quinol.

GO - Molecular functioni

Keywordsi

Molecular functionOxidoreductase
LigandNAD

Enzyme and pathway databases

BioCyciTTHE300852:G1GKC-513-MONOMER

Protein family/group databases

TCDBi3.D.1.3.1 the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family

Names & Taxonomyi

Protein namesi
Recommended name:
NADH-quinone oxidoreductase subunit 15 (EC:1.6.5.11)
Alternative name(s):
NADH dehydrogenase I chain 15
NDH-1 subunit 15
Gene namesi
Name:nqo15
Ordered Locus Names:TTHA0496
OrganismiThermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Taxonomic identifieri300852 [NCBI]
Taxonomic lineageiBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus
Proteomesi
  • UP000000532 Componenti: Chromosome

Subcellular locationi

  • Cell membrane 1 Publication; Peripheral membrane protein 1 Publication; Cytoplasmic side 1 Publication

GO - Cellular componenti

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00002330142 – 129NADH-quinone oxidoreductase subunit 15Add BLAST128

Keywords - PTMi

Quinone

Interactioni

Subunit structurei

NDH-1 is composed of 15 different subunits, Nqo1 to Nqo15. The complex has a L-shaped structure, with the hydrophobic arm (subunits Nqo7, Nqo8 and Nqo10 to Nqo14) embedded in the membrane and the hydrophilic peripheral arm (subunits Nqo1 to Nqo6, Nqo9 and Nqo15) protruding into the bacterial cytoplasm. The hydrophilic domain contains all the redox centers. Nqo15 is bound to the side of the complex near the N-terminus of Nqo3, where it interacts with subunits Nqo3, Nqo2, Nqo1, Nqo9 and Nqo4.2 Publications

Protein-protein interaction databases

DIPiDIP-59273N
IntActiQ5SKZ7, 1 interactor
STRINGi300852.TTHA0496

Structurei

Secondary structure

1129
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi5 – 26Combined sources22
Beta strandi31 – 36Combined sources6
Helixi38 – 41Combined sources4
Beta strandi53 – 60Combined sources8
Beta strandi62 – 64Combined sources3
Beta strandi66 – 72Combined sources7
Beta strandi75 – 79Combined sources5
Beta strandi82 – 86Combined sources5
Turni87 – 90Combined sources4
Beta strandi91 – 98Combined sources8
Turni99 – 101Combined sources3
Beta strandi102 – 105Combined sources4
Helixi112 – 126Combined sources15

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2FUGX-ray3.307/H/Q/Z1-129[»]
2YBBelectron microscopy19.0071-129[»]
3I9VX-ray3.107/H1-129[»]
3IAMX-ray3.107/H1-129[»]
3IASX-ray3.157/H/Q/Z1-129[»]
3M9SX-ray4.507/J1-129[»]
4HEAX-ray3.307/I1-129[»]
ProteinModelPortaliQ5SKZ7
SMRiQ5SKZ7
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ5SKZ7

Family & Domainsi

Domaini

Has a similar fold to the mitochondrial iron chaperone frataxin.1 Publication

Sequence similaritiesi

Belongs to the complex I Nqo15 family.Curated

Phylogenomic databases

eggNOGiENOG4106YGA Bacteria
ENOG410YSNB LUCA
HOGENOMiHOG000099234
OMAiIYRMERP

Family and domain databases

Gene3Di3.30.920.80, 1 hit
InterProiView protein in InterPro
IPR021093 NADH_quinone_OxRdtase_su15
IPR038458 NADH_quinone_OxRdtase_su15_sf
PfamiView protein in Pfam
PF11497 NADH_Oxid_Nqo15, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q5SKZ7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSASSERELY EAWVELLSWM REYAQAKGVR FEKEADFPDF IYRMERPYDL
60 70 80 90 100
PTTIMTASLS DGLGEPFLLA DVSPRHAKLK RIGLRLPRAH IHLHAHYEPG
110 120
KGLVTGKIPL TKERFFALAD RAREALAFA
Length:129
Mass (Da):14,788
Last modified:January 23, 2007 - v3
Checksum:iA944E174C0DE152C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP008226 Genomic DNA Translation: BAD70319.1
RefSeqiWP_011227977.1, NC_006461.1
YP_143762.1, NC_006461.1

Genome annotation databases

EnsemblBacteriaiBAD70319; BAD70319; BAD70319
GeneIDi3169322
KEGGittj:TTHA0496
PATRICifig|300852.9.peg.494

Similar proteinsi

Entry informationi

Entry nameiNQO15_THET8
AccessioniPrimary (citable) accession number: Q5SKZ7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 18, 2006
Last sequence update: January 23, 2007
Last modified: March 28, 2018
This is version 83 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome
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Main funding by: National Institutes of Health