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Q5SKZ7 (NQO15_THET8) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 62. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
NADH-quinone oxidoreductase subunit 15

EC=1.6.99.5
Alternative name(s):
NADH dehydrogenase I chain 15
NDH-1 subunit 15
Gene names
Name:nqo15
Ordered Locus Names:TTHA0496
OrganismThermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) [Reference proteome] [HAMAP]
Taxonomic identifier300852 [NCBI]
Taxonomic lineageBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus

Protein attributes

Sequence length129 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is menaquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient required for the synthesis of ATP. The nqo15 subunit has probably a role in complex stabilization, and may be also involved in the storage of iron for iron-sulfur cluster regeneration in the complex. Ref.3

Catalytic activity

NADH + quinone = NAD+ + quinol.

Subunit structure

NDH-1 is composed of 15 different subunits, nqo1 to nqo15. The complex has a L-shaped structure, with the hydrophobic arm (subunits nqo7, nqo8 and nqo10 to nqo14) embedded in the membrane and the hydrophilic peripheral arm (subunits nqo1 to nqo6, nqo9 and nqo15) protruding into the bacterial cytoplasm. The hydrophilic domain contains all the redox centers. Nqo15 is bound to the side of the complex near the N-terminus of nqo3, where it interacts with subunits nqo3, nqo2, nqo1, nqo9 and nqo4. Ref.2 Ref.3

Subcellular location

Cell membrane; Peripheral membrane protein; Cytoplasmic side.

Domain

Has a similar fold to the mitochondrial iron chaperone frataxin.

Sequence similarities

Belongs to the complex I nqo15 family.

Ontologies

Keywords
   Cellular componentCell membrane
Membrane
   LigandNAD
   Molecular functionOxidoreductase
   PTMQuinone
   Technical term3D-structure
Complete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processiron-sulfur cluster assembly

Inferred from electronic annotation. Source: InterPro

   Cellular_componentplasma membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionNADH dehydrogenase (quinone) activity

Inferred from electronic annotation. Source: UniProtKB-EC

ferric iron binding

Inferred from electronic annotation. Source: InterPro

quinone binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.2
Chain2 – 129128NADH-quinone oxidoreductase subunit 15
PRO_0000233014

Secondary structure

....................... 129
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q5SKZ7 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: A944E174C0DE152C

FASTA12914,788
        10         20         30         40         50         60 
MSASSERELY EAWVELLSWM REYAQAKGVR FEKEADFPDF IYRMERPYDL PTTIMTASLS 

        70         80         90        100        110        120 
DGLGEPFLLA DVSPRHAKLK RIGLRLPRAH IHLHAHYEPG KGLVTGKIPL TKERFFALAD 


RAREALAFA 

« Hide

References

« Hide 'large scale' references
[1]"Complete genome sequence of Thermus thermophilus HB8."
Masui R., Kurokawa K., Nakagawa N., Tokunaga F., Koyama Y., Shibata T., Oshima T., Yokoyama S., Yasunaga T., Kuramitsu S.
Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: HB8 / ATCC 27634 / DSM 579.
[2]"Identification of a novel subunit of respiratory complex I from Thermus thermophilus."
Hinchliffe P., Carroll J., Sazanov L.A.
Biochemistry 45:4413-4420(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 2-9, CHARACTERIZATION, IDENTIFICATION BY MASS SPECTROMETRY, SUBUNIT.
[3]"Structure of the hydrophilic domain of respiratory complex I from Thermus thermophilus."
Sazanov L.A., Hinchliffe P.
Science 311:1430-1436(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (3.3 ANGSTROMS), FUNCTION, SUBUNIT, ELECTRON TRANSFER MECHANISM.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AP008226 Genomic DNA. Translation: BAD70319.1.
RefSeqYP_143762.1. NC_006461.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2FUGX-ray3.307/H/Q/Z1-129[»]
2YBBelectron microscopy19.0071-129[»]
3I9VX-ray3.107/H1-129[»]
3IAMX-ray3.107/H1-129[»]
3IASX-ray3.157/H/Q/Z1-129[»]
3M9SX-ray4.507/J1-129[»]
4HEAX-ray3.307/I1-129[»]
ProteinModelPortalQ5SKZ7.
SMRQ5SKZ7. Positions 3-129.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-59273N.
STRING300852.TTHA0496.

Protein family/group databases

TCDB3.D.1.3.1. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAD70319; BAD70319; BAD70319.
GeneID3169322.
KEGGttj:TTHA0496.
PATRIC23955967. VBITheThe93045_0494.

Phylogenomic databases

eggNOGNOG43023.
HOGENOMHOG000099234.
OMAYIYRMER.
OrthoDBEOG6NGVT2.

Enzyme and pathway databases

BioCycTTHE300852:GH8R-521-MONOMER.

Family and domain databases

InterProIPR002908. Frataxin/CyaY.
IPR021093. NADH_quinone_OxRdtase_su15.
[Graphical view]
PfamPF11497. NADH_Oxid_Nqo15. 1 hit.
[Graphical view]
SUPFAMSSF55387. SSF55387. 1 hit.
ProtoNetSearch...

Other

EvolutionaryTraceQ5SKZ7.

Entry information

Entry nameNQO15_THET8
AccessionPrimary (citable) accession number: Q5SKZ7
Entry history
Integrated into UniProtKB/Swiss-Prot: April 18, 2006
Last sequence update: January 23, 2007
Last modified: May 14, 2014
This is version 62 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references