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Q5SKZ7

- NQO15_THET8

UniProt

Q5SKZ7 - NQO15_THET8

Protein

NADH-quinone oxidoreductase subunit 15

Gene

nqo15

Organism
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 64 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is menaquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient required for the synthesis of ATP. The nqo15 subunit has probably a role in complex stabilization, and may be also involved in the storage of iron for iron-sulfur cluster regeneration in the complex.1 Publication

    Catalytic activityi

    NADH + quinone = NAD+ + quinol.

    GO - Molecular functioni

    1. ferric iron binding Source: InterPro
    2. NADH dehydrogenase (quinone) activity Source: UniProtKB-EC
    3. quinone binding Source: UniProtKB-KW

    GO - Biological processi

    1. iron-sulfur cluster assembly Source: InterPro

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Ligandi

    NAD

    Enzyme and pathway databases

    BioCyciTTHE300852:GH8R-521-MONOMER.

    Protein family/group databases

    TCDBi3.D.1.3.1. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    NADH-quinone oxidoreductase subunit 15 (EC:1.6.99.5)
    Alternative name(s):
    NADH dehydrogenase I chain 15
    NDH-1 subunit 15
    Gene namesi
    Name:nqo15
    Ordered Locus Names:TTHA0496
    OrganismiThermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
    Taxonomic identifieri300852 [NCBI]
    Taxonomic lineageiBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus
    ProteomesiUP000000532: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    1. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 129128NADH-quinone oxidoreductase subunit 15PRO_0000233014Add
    BLAST

    Keywords - PTMi

    Quinone

    Interactioni

    Subunit structurei

    NDH-1 is composed of 15 different subunits, nqo1 to nqo15. The complex has a L-shaped structure, with the hydrophobic arm (subunits nqo7, nqo8 and nqo10 to nqo14) embedded in the membrane and the hydrophilic peripheral arm (subunits nqo1 to nqo6, nqo9 and nqo15) protruding into the bacterial cytoplasm. The hydrophilic domain contains all the redox centers. Nqo15 is bound to the side of the complex near the N-terminus of nqo3, where it interacts with subunits nqo3, nqo2, nqo1, nqo9 and nqo4.2 Publications

    Protein-protein interaction databases

    DIPiDIP-59273N.
    STRINGi300852.TTHA0496.

    Structurei

    Secondary structure

    1
    129
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi5 – 2622
    Beta strandi31 – 366
    Helixi38 – 414
    Beta strandi53 – 608
    Beta strandi62 – 643
    Beta strandi66 – 727
    Beta strandi75 – 795
    Beta strandi82 – 865
    Turni87 – 904
    Beta strandi91 – 988
    Turni99 – 1013
    Beta strandi102 – 1054
    Helixi112 – 12615

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2FUGX-ray3.307/H/Q/Z1-129[»]
    2YBBelectron microscopy19.0071-129[»]
    3I9VX-ray3.107/H1-129[»]
    3IAMX-ray3.107/H1-129[»]
    3IASX-ray3.157/H/Q/Z1-129[»]
    3M9SX-ray4.507/J1-129[»]
    4HEAX-ray3.307/I1-129[»]
    ProteinModelPortaliQ5SKZ7.
    SMRiQ5SKZ7. Positions 3-129.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ5SKZ7.

    Family & Domainsi

    Domaini

    Has a similar fold to the mitochondrial iron chaperone frataxin.

    Sequence similaritiesi

    Belongs to the complex I nqo15 family.Curated

    Phylogenomic databases

    eggNOGiNOG43023.
    HOGENOMiHOG000099234.
    OMAiYIYRMER.
    OrthoDBiEOG6NGVT2.

    Family and domain databases

    InterProiIPR002908. Frataxin/CyaY.
    IPR021093. NADH_quinone_OxRdtase_su15.
    [Graphical view]
    PfamiPF11497. NADH_Oxid_Nqo15. 1 hit.
    [Graphical view]
    SUPFAMiSSF55387. SSF55387. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q5SKZ7-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSASSERELY EAWVELLSWM REYAQAKGVR FEKEADFPDF IYRMERPYDL    50
    PTTIMTASLS DGLGEPFLLA DVSPRHAKLK RIGLRLPRAH IHLHAHYEPG 100
    KGLVTGKIPL TKERFFALAD RAREALAFA 129
    Length:129
    Mass (Da):14,788
    Last modified:January 23, 2007 - v3
    Checksum:iA944E174C0DE152C
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AP008226 Genomic DNA. Translation: BAD70319.1.
    RefSeqiWP_011227977.1. NC_006461.1.
    YP_143762.1. NC_006461.1.

    Genome annotation databases

    EnsemblBacteriaiBAD70319; BAD70319; BAD70319.
    GeneIDi3169322.
    KEGGittj:TTHA0496.
    PATRICi23955967. VBITheThe93045_0494.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AP008226 Genomic DNA. Translation: BAD70319.1 .
    RefSeqi WP_011227977.1. NC_006461.1.
    YP_143762.1. NC_006461.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2FUG X-ray 3.30 7/H/Q/Z 1-129 [» ]
    2YBB electron microscopy 19.00 7 1-129 [» ]
    3I9V X-ray 3.10 7/H 1-129 [» ]
    3IAM X-ray 3.10 7/H 1-129 [» ]
    3IAS X-ray 3.15 7/H/Q/Z 1-129 [» ]
    3M9S X-ray 4.50 7/J 1-129 [» ]
    4HEA X-ray 3.30 7/I 1-129 [» ]
    ProteinModelPortali Q5SKZ7.
    SMRi Q5SKZ7. Positions 3-129.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-59273N.
    STRINGi 300852.TTHA0496.

    Protein family/group databases

    TCDBi 3.D.1.3.1. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAD70319 ; BAD70319 ; BAD70319 .
    GeneIDi 3169322.
    KEGGi ttj:TTHA0496.
    PATRICi 23955967. VBITheThe93045_0494.

    Phylogenomic databases

    eggNOGi NOG43023.
    HOGENOMi HOG000099234.
    OMAi YIYRMER.
    OrthoDBi EOG6NGVT2.

    Enzyme and pathway databases

    BioCyci TTHE300852:GH8R-521-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei Q5SKZ7.

    Family and domain databases

    InterProi IPR002908. Frataxin/CyaY.
    IPR021093. NADH_quinone_OxRdtase_su15.
    [Graphical view ]
    Pfami PF11497. NADH_Oxid_Nqo15. 1 hit.
    [Graphical view ]
    SUPFAMi SSF55387. SSF55387. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Complete genome sequence of Thermus thermophilus HB8."
      Masui R., Kurokawa K., Nakagawa N., Tokunaga F., Koyama Y., Shibata T., Oshima T., Yokoyama S., Yasunaga T., Kuramitsu S.
      Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: HB8 / ATCC 27634 / DSM 579.
    2. "Identification of a novel subunit of respiratory complex I from Thermus thermophilus."
      Hinchliffe P., Carroll J., Sazanov L.A.
      Biochemistry 45:4413-4420(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 2-9, CHARACTERIZATION, IDENTIFICATION BY MASS SPECTROMETRY, SUBUNIT.
    3. "Structure of the hydrophilic domain of respiratory complex I from Thermus thermophilus."
      Sazanov L.A., Hinchliffe P.
      Science 311:1430-1436(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (3.3 ANGSTROMS), FUNCTION, SUBUNIT, ELECTRON TRANSFER MECHANISM.

    Entry informationi

    Entry nameiNQO15_THET8
    AccessioniPrimary (citable) accession number: Q5SKZ7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 18, 2006
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 64 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3