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Protein

Glycine cleavage system H protein

Gene

gcvH

Organism
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein.UniRule annotation

Cofactori

(R)-lipoateUniRule annotation1 PublicationNote: Binds 1 lipoyl cofactor covalently.UniRule annotation1 Publication

GO - Biological processi

Complete GO annotation...

Enzyme and pathway databases

BioCyciTTHE300852:GH8R-549-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Glycine cleavage system H proteinUniRule annotation
Gene namesi
Name:gcvHUniRule annotation
Ordered Locus Names:TTHA0524
OrganismiThermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Taxonomic identifieri300852 [NCBI]
Taxonomic lineageiBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus
Proteomesi
  • UP000000532 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 128128Glycine cleavage system H proteinPRO_0000302458Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei63 – 631N6-lipoyllysineUniRule annotation1 Publication

Interactioni

Subunit structurei

The glycine cleavage system is composed of four proteins: P, T, L and H (By similarity). Monomer.UniRule annotation1 Publication

Protein-protein interaction databases

STRINGi300852.TTHA0524.

Structurei

Secondary structure

1
128
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi5 – 95Combined sources
Beta strandi13 – 197Combined sources
Beta strandi22 – 276Combined sources
Helixi29 – 357Combined sources
Beta strandi37 – 426Combined sources
Beta strandi55 – 6915Combined sources
Beta strandi71 – 799Combined sources
Helixi82 – 854Combined sources
Helixi89 – 924Combined sources
Turni94 – 985Combined sources
Beta strandi101 – 1066Combined sources
Helixi108 – 1136Combined sources
Helixi117 – 12610Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1ONLX-ray2.50A/B/C1-128[»]
ProteinModelPortaliQ5SKW9.
SMRiQ5SKW9. Positions 2-128.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ5SKW9.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini22 – 10483Lipoyl-bindingPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the GcvH family.UniRule annotation
Contains 1 lipoyl-binding domain.UniRule annotation

Keywords - Domaini

Lipoyl

Phylogenomic databases

eggNOGiENOG4105KE9. Bacteria.
COG0509. LUCA.
HOGENOMiHOG000239392.
KOiK02437.
OMAiNTDPYGE.
OrthoDBiEOG60CWTC.
PhylomeDBiQ5SKW9.

Family and domain databases

HAMAPiMF_00272. GcvH.
InterProiIPR003016. 2-oxoA_DH_lipoyl-BS.
IPR000089. Biotin_lipoyl.
IPR002930. GCV_H.
IPR017453. GCV_H_sub.
IPR011053. Single_hybrid_motif.
[Graphical view]
PANTHERiPTHR11715. PTHR11715. 1 hit.
PfamiPF01597. GCV_H. 1 hit.
[Graphical view]
SUPFAMiSSF51230. SSF51230. 1 hit.
TIGRFAMsiTIGR00527. gcvH. 1 hit.
PROSITEiPS50968. BIOTINYL_LIPOYL. 1 hit.
PS00189. LIPOYL. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5SKW9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDIPKDRFYT KTHEWALPEG DTVLVGITDY AQDALGDVVY VELPEVGRVV
60 70 80 90 100
EKGEAVAVVE SVKTASDIYA PVAGEIVEVN LALEKTPELV NQDPYGEGWI
110 120
FRLKPRDMGD LDELLDAGGY QEVLESEA
Length:128
Mass (Da):14,083
Last modified:December 21, 2004 - v1
Checksum:i9A93A6CA42B4DD4E
GO

Mass spectrometryi

Molecular mass is 14083 Da from positions 1 - 128. Determined by MALDI. Unlipoylated protein.1 Publication
Molecular mass is 14273 Da from positions 1 - 128. Determined by MALDI. Lipoylated protein.1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP008226 Genomic DNA. Translation: BAD70347.1.
RefSeqiWP_011228002.1. NC_006461.1.
YP_143790.1. NC_006461.1.

Genome annotation databases

EnsemblBacteriaiBAD70347; BAD70347; BAD70347.
GeneIDi3169107.
KEGGittj:TTHA0524.
PATRICi23956023. VBITheThe93045_0522.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP008226 Genomic DNA. Translation: BAD70347.1.
RefSeqiWP_011228002.1. NC_006461.1.
YP_143790.1. NC_006461.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1ONLX-ray2.50A/B/C1-128[»]
ProteinModelPortaliQ5SKW9.
SMRiQ5SKW9. Positions 2-128.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi300852.TTHA0524.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAD70347; BAD70347; BAD70347.
GeneIDi3169107.
KEGGittj:TTHA0524.
PATRICi23956023. VBITheThe93045_0522.

Phylogenomic databases

eggNOGiENOG4105KE9. Bacteria.
COG0509. LUCA.
HOGENOMiHOG000239392.
KOiK02437.
OMAiNTDPYGE.
OrthoDBiEOG60CWTC.
PhylomeDBiQ5SKW9.

Enzyme and pathway databases

BioCyciTTHE300852:GH8R-549-MONOMER.

Miscellaneous databases

EvolutionaryTraceiQ5SKW9.

Family and domain databases

HAMAPiMF_00272. GcvH.
InterProiIPR003016. 2-oxoA_DH_lipoyl-BS.
IPR000089. Biotin_lipoyl.
IPR002930. GCV_H.
IPR017453. GCV_H_sub.
IPR011053. Single_hybrid_motif.
[Graphical view]
PANTHERiPTHR11715. PTHR11715. 1 hit.
PfamiPF01597. GCV_H. 1 hit.
[Graphical view]
SUPFAMiSSF51230. SSF51230. 1 hit.
TIGRFAMsiTIGR00527. gcvH. 1 hit.
PROSITEiPS50968. BIOTINYL_LIPOYL. 1 hit.
PS00189. LIPOYL. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Complete genome sequence of Thermus thermophilus HB8."
    Masui R., Kurokawa K., Nakagawa N., Tokunaga F., Koyama Y., Shibata T., Oshima T., Yokoyama S., Yasunaga T., Kuramitsu S.
    Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: HB8 / ATCC 27634 / DSM 579.
  2. "Structure of Thermus thermophilus HB8 H-protein of the glycine-cleavage system, resolved by a six-dimensional molecular-replacement method."
    Nakai T., Ishijima J., Masui R., Kuramitsu S., Kamiya N.
    Acta Crystallogr. D 59:1610-1618(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS), COFACTOR, MASS SPECTROMETRY, SUBUNIT, LIPOYLATION AT LYS-63.

Entry informationi

Entry nameiGCSH_THET8
AccessioniPrimary (citable) accession number: Q5SKW9
Secondary accession number(s): P83697
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: December 21, 2004
Last modified: November 11, 2015
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.