Q5SKU2 (IF3_THET8) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Translation initiation factor IF-3

Gene names

Name:	infC
Ordered Locus Names:	TTHA0551

Organism

Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) [Reference proteome] [HAMAP]

Taxonomic identifier

300852 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Deinococcus-Thermus › Deinococci › Thermales › Thermaceae › Thermus › Thermus thermophilus

Protein attributes

Sequence length	171 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	IF-3 binds to the 30S ribosomal subunit and shifts the equilibrum between 70S ribosomes and their 50S and 30S subunits in favor of the free subunits, thus enhancing the availability of 30S subunits on which protein synthesis initiation begins. HAMAP-Rule MF_00080
Subunit structure	Monomer. Binds to proteins S7, S11 and S18 of the small ribosomal subunit. Ref.2
Subcellular location	Cytoplasm HAMAP-Rule MF_00080.
Sequence similarities	Belongs to the IF-3 family.
Sequence caution	The sequence BAD70374.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Molecular function	Initiation factor
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	translation initiation factor activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 171	171	Translation initiation factor IF-3 HAMAP-Rule MF_00080		PRO_0000280033

Sequences

	Sequence		Length	Mass (Da)
	Q5SKU2 [UniParc]. Last modified March 6, 2007. Version 2. Checksum: 55954FFC1E365E29	FASTA	171	19,866
	10 20 30 40 50 60 MKEYLTNERI RAKQVRVVGP DGKQLGIMDT REALRLAQEM DLDLVLVGPN ADPPVARIMD 70 80 90 100 110 120 YSKWRYEQQM AEKEARKKAK RTEVKSIKFR VKIDEHDYQT KLGHIKRFLQ EGHKVKVTIM 130 140 150 160 170 FRGREVAHPE LGERILNRVT EDLKDLAVVE MKPEMLGRDM NMLLAPVKVS A

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete genome sequence of Thermus thermophilus HB8." Masui R., Kurokawa K., Nakagawa N., Tokunaga F., Koyama Y., Shibata T., Oshima T., Yokoyama S., Yasunaga T., Kuramitsu S. Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: HB8 / ATCC 27634 / DSM 579.
[2]	"Crystal structures of complexes of the small ribosomal subunit with tetracycline, edeine and IF3." Pioletti M., Schluenzen F., Harms J., Zarivach R., Gluehmann M., Avila H., Bashan A., Bartels H., Auerbach T., Jacobi C., Hartsch T., Yonath A., Franceschi F. EMBO J. 20:1829-1839(2001) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (3.2 ANGSTROMS) OF 83-171 WITH THE 30S RIBOSOMAL SUBUNIT.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AP008226 Genomic DNA. Translation: BAD70374.1. Different initiation.

RefSeq

YP_143817.1. NC_006461.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1I96	X-ray	4.20	V	83-171	[»]

ProteinModelPortal

Q5SKU2.

SMR

Q5SKU2. Positions 2-77.

ModBase

Search...

Protein-protein interaction databases

STRING

300852.TTHA0551.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

BAD70374; BAD70374; BAD70374.

GeneID

3168619.

KEGG

ttj:TTHA0551.

PATRIC

23956079. VBITheThe93045_0550.

Phylogenomic databases

eggNOG

COG0290.

HOGENOM

HOG000035157.

KO

K02520.

OMA

HDYQTKV.

ProtClustDB

PRK00028.

Family and domain databases

Gene3D

3.10.20.80. 1 hit.

HAMAP

MF_00080. IF-3.

InterPro

IPR001288. Translation_initiation_fac_3.
IPR019815. Translation_initiation_fac_3_C.
IPR019814. Translation_initiation_fac_3_N.
[Graphical view]

PANTHER

PTHR10938. PTHR10938. 1 hit.

Pfam

PF00707. IF3_C. 1 hit.
PF05198. IF3_N. 1 hit.
[Graphical view]

SUPFAM

SSF54364. IF3. 1 hit.
SSF55200. IF3. 1 hit.

TIGRFAMs

TIGR00168. infC. 1 hit.

PROSITE

PS00938. IF3. False negative.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

Q5SKU2.

Entry information

Entry name

IF3_THET8

Accession

Primary (citable) accession number: Q5SKU2

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 6, 2007
Last sequence update:	March 6, 2007
Last modified:	May 1, 2013
This is version 56 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program