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Protein

tRNA (adenine(58)-N(1))-methyltransferase TrmI

Gene

trmI

Organism
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the S-adenosyl-L-methionine-dependent formation of N(1)-methyladenine at position 58 (m1A58) in tRNA. Is required for cell growth at extreme temperatures (By similarity).By similarity

Catalytic activityi

S-adenosyl-L-methionine + adenine(58) in tRNA = S-adenosyl-L-homocysteine + N(1)-methyladenine(58) in tRNA.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei125S-adenosyl-L-methioninePROSITE-ProRule annotation1
Binding sitei130S-adenosyl-L-methioninePROSITE-ProRule annotation1
Binding sitei155S-adenosyl-L-methioninePROSITE-ProRule annotation1
Binding sitei170S-adenosyl-L-methioninePROSITE-ProRule annotation1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (adenine(58)-N(1))-methyltransferase TrmI (EC:2.1.1.220)
Alternative name(s):
tRNA(m1A58)-methyltransferase
Short name:
tRNA(m1A58)MTase
Gene namesi
Name:trmI
Ordered Locus Names:TTHA0609
OrganismiThermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Taxonomic identifieri300852 [NCBI]
Taxonomic lineageiBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus
Proteomesi
  • UP000000532 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003118131 – 255tRNA (adenine(58)-N(1))-methyltransferase TrmIAdd BLAST255

Interactioni

Subunit structurei

Homotetramer composed of a dimer of dimers.By similarity

Protein-protein interaction databases

STRINGi300852.TTHA0609.

Structurei

3D structure databases

ProteinModelPortaliQ5SKN4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni104 – 107S-adenosyl-L-methionine bindingBy similarity4

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. TRM61 family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG41066UU. Bacteria.
COG2519. LUCA.
HOGENOMiHOG000245274.
KOiK07442.
OMAiRPDHRMI.
PhylomeDBiQ5SKN4.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR029063. SAM-dependent_MTases.
IPR014816. tRNA_MeTrfase_Gcd14.
[Graphical view]
PfamiPF08704. GCD14. 1 hit.
[Graphical view]
PIRSFiPIRSF017269. GCD14. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS51620. SAM_TRM61. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5SKN4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAWPGPLLLK DRKGRAYLVF PKEGGVFHHH KGSVPHEALL EAGPGGVVRT
60 70 80 90 100
HLGEELSVHR PTLEEYLLHM KRSATPTYPK DASAMVTLLD LAPGMRVLEA
110 120 130 140 150
GTGSGGLTLF LARAVGEKGL VESYEARPHH LAQAERNVRA FWQVENVRFH
160 170 180 190 200
LGKLEEAELE EAAYDGVALD LMEPWKALEK AALALKPDRF LVAYLPNITQ
210 220 230 240 250
VLELVRAAEA HPFRLERVLE VGWREWEVRL PVAHPRFQQV GHTAFLVALR

RWKAS
Length:255
Mass (Da):28,568
Last modified:December 21, 2004 - v1
Checksum:i0226E3502232BC5A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP008226 Genomic DNA. Translation: BAD70432.1.
RefSeqiWP_011228067.1. NC_006461.1.
YP_143875.1. NC_006461.1.

Genome annotation databases

EnsemblBacteriaiBAD70432; BAD70432; BAD70432.
GeneIDi3169400.
KEGGittj:TTHA0609.
PATRICi23956193. VBITheThe93045_0607.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP008226 Genomic DNA. Translation: BAD70432.1.
RefSeqiWP_011228067.1. NC_006461.1.
YP_143875.1. NC_006461.1.

3D structure databases

ProteinModelPortaliQ5SKN4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi300852.TTHA0609.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAD70432; BAD70432; BAD70432.
GeneIDi3169400.
KEGGittj:TTHA0609.
PATRICi23956193. VBITheThe93045_0607.

Phylogenomic databases

eggNOGiENOG41066UU. Bacteria.
COG2519. LUCA.
HOGENOMiHOG000245274.
KOiK07442.
OMAiRPDHRMI.
PhylomeDBiQ5SKN4.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR029063. SAM-dependent_MTases.
IPR014816. tRNA_MeTrfase_Gcd14.
[Graphical view]
PfamiPF08704. GCD14. 1 hit.
[Graphical view]
PIRSFiPIRSF017269. GCD14. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS51620. SAM_TRM61. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTRMI_THET8
AccessioniPrimary (citable) accession number: Q5SKN4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 4, 2007
Last sequence update: December 21, 2004
Last modified: November 2, 2016
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.