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Protein

Ribosomal RNA small subunit methyltransferase E

Gene

TTHA0657

Organism
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Status
Unreviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Specifically methylates the N3 position of the uracil ring of uridine 1498 (m3U1498) in 16S rRNA. Acts on the fully assembled 30S ribosomal subunit.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + uracil(1498) in 16S rRNA = S-adenosyl-L-homocysteine + N3-methyluracil(1498) in 16S rRNA.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi158Iron-sulfur (4Fe-4S-S-AdoMet)Combined sources1
Metal bindingi180Iron-sulfur (4Fe-4S-S-AdoMet)Combined sources1
Metal bindingi181Iron-sulfur (4Fe-4S-S-AdoMet)Combined sources1
Metal bindingi182Iron-sulfur (4Fe-4S-S-AdoMet); via carbonyl oxygenCombined sources1
Metal bindingi183Iron-sulfur (4Fe-4S-S-AdoMet); via carbonyl oxygenCombined sources1
Metal bindingi184Iron-sulfur (4Fe-4S-S-AdoMet); via carbonyl oxygenCombined sources1
Metal bindingi204Iron-sulfur (4Fe-4S-S-AdoMet); via amide nitrogenCombined sources1
Metal bindingi207Iron-sulfur (4Fe-4S-S-AdoMet); via carbonyl oxygenCombined sources1
Metal bindingi209Iron-sulfur (4Fe-4S-S-AdoMet); via amide nitrogen and carbonyl oxygenCombined sources1
Metal bindingi214Iron-sulfur (4Fe-4S-S-AdoMet)Combined sources1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMethyltransferaseUniRule annotation, Transferase
Biological processrRNA processingUniRule annotation
Ligand4Fe-4SCombined sources, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionineUniRule annotation

Enzyme and pathway databases

BioCyciTTHE300852:GH8R-671-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribosomal RNA small subunit methyltransferase EUniRule annotation (EC:2.1.1.193UniRule annotation)
Gene namesi
Ordered Locus Names:TTHA0657Imported
OrganismiThermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)Imported
Taxonomic identifieri300852 [NCBI]
Taxonomic lineageiBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus
Proteomesi
  • UP000000532 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

CytoplasmUniRule annotation

Interactioni

Protein-protein interaction databases

STRINGi300852.TTHA0657.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1V6ZX-ray2.00A/B1-228[»]
2CX8X-ray2.53A/B1-228[»]
2Z0YX-ray2.80A/B1-228[»]
ProteinModelPortaliQ5SKI6.
SMRiQ5SKI6.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ5SKI6.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni158 – 159S-adenosyl-L-homocysteine bindingCombined sources2
Regioni181 – 184S-adenosyl-L-homocysteine bindingCombined sources4
Regioni204 – 209S-adenosyl-L-homocysteine bindingCombined sources6

Sequence similaritiesi

Belongs to the RNA methyltransferase RsmE family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105QF0. Bacteria.
COG1385. LUCA.
HOGENOMiHOG000015266.
KOiK09761.
OMAiERMEFTI.
PhylomeDBiQ5SKI6.

Family and domain databases

Gene3Di3.40.1280.10. 1 hit.
InterProiView protein in InterPro
IPR029028. Alpha/beta_knot_MTases.
IPR015947. PUA-like_sf.
IPR006700. rRNA_ssu_MeTrfase-E.
IPR029026. tRNA_m1G_MTases_N.
PANTHERiPTHR30027. PTHR30027. 1 hit.
PfamiView protein in Pfam
PF04452. Methyltrans_RNA. 1 hit.
PIRSFiPIRSF015601. MTase_slr0722. 1 hit.
SUPFAMiSSF75217. SSF75217. 1 hit.
SSF88697. SSF88697. 1 hit.
TIGRFAMsiTIGR00046. TIGR00046. 1 hit.

Sequencei

Sequence statusi: Complete.

Q5SKI6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRPHRAFSPG LTGVLPLRET RHLVEVLRAR VGDRFTVFDG EREALAEVVD
60 70 80 90 100
LGPPLRYRVL EERRPEREVG VEVVLYVALL KGDKLAEVVR AATELGATRI
110 120 130 140 150
QPLVTRHSVP KEMGEGKLRR LRAVALEAAK QSGRVVVPEV LPPIPLKAVP
160 170 180 190 200
QVAQGLVAHV GATARVREVL DPEKPLALAV GPEGGFAEEE VALLEARGFT
210 220
PVSLGRRILR AETAALALLA LCTAGEGR
Length:228
Mass (Da):24,671
Last modified:December 21, 2004 - v1
Checksum:i3533C109242B3190
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP008226 Genomic DNA. Translation: BAD70480.1.
RefSeqiWP_011228101.1. NC_006461.1.
YP_143923.1. NC_006461.1.

Genome annotation databases

EnsemblBacteriaiBAD70480; BAD70480; BAD70480.
GeneIDi3169973.
KEGGittj:TTHA0657.
PATRICifig|300852.9.peg.651.

Similar proteinsi

Entry informationi

Entry nameiQ5SKI6_THET8
AccessioniPrimary (citable) accession number: Q5SKI6
Entry historyiIntegrated into UniProtKB/TrEMBL: December 21, 2004
Last sequence update: December 21, 2004
Last modified: November 22, 2017
This is version 91 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

3D-structureCombined sources, Complete proteome, Reference proteomeImported