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Protein

Thiazole synthase

Gene

thiG

Organism
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the rearrangement of 1-deoxy-D-xylulose 5-phosphate (DXP) to produce the thiazole phosphate moiety of thiamine. Sulfur is provided by the thiocarboxylate moiety of the carrier protein ThiS. In vitro, sulfur can be provided by H2S.UniRule annotation

Catalytic activityi

1-deoxy-D-xylulose 5-phosphate + 2-iminoacetate + thiocarboxy-[sulfur-carrier protein ThiS] = 2-((2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene)ethyl phosphate + [sulfur-carrier protein ThiS] + 2 H2O.UniRule annotation

Pathwayi: thiamine diphosphate biosynthesis

This protein is involved in the pathway thiamine diphosphate biosynthesis, which is part of Cofactor biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway thiamine diphosphate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei96Schiff-base intermediate with DXPUniRule annotation1
Binding sitei157DXP; via amide nitrogenUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Thiamine biosynthesis

Keywords - Ligandi

Schiff base

Enzyme and pathway databases

UniPathwayiUPA00060.

Names & Taxonomyi

Protein namesi
Recommended name:
Thiazole synthaseUniRule annotation (EC:2.8.1.10UniRule annotation)
Gene namesi
Name:thiGUniRule annotation
Ordered Locus Names:TTHA0676
OrganismiThermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Taxonomic identifieri300852 [NCBI]
Taxonomic lineageiBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus
Proteomesi
  • UP000000532 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001628691 – 268Thiazole synthaseAdd BLAST268

Interactioni

Subunit structurei

Homotetramer. Forms heterodimers with either ThiH or ThiS.UniRule annotation

Protein-protein interaction databases

STRINGi300852.TTHA0676.

Structurei

Secondary structure

1268
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi4 – 6Combined sources3
Beta strandi9 – 11Combined sources3
Beta strandi14 – 18Combined sources5
Helixi25 – 34Combined sources10
Beta strandi38 – 46Combined sources9
Helixi57 – 60Combined sources4
Turni61 – 63Combined sources3
Beta strandi64 – 69Combined sources6
Helixi76 – 90Combined sources15
Beta strandi93 – 96Combined sources4
Turni103 – 105Combined sources3
Helixi110 – 122Combined sources13
Helixi135 – 144Combined sources10
Beta strandi149 – 155Combined sources7
Turni156 – 158Combined sources3
Helixi165 – 173Combined sources9
Turni174 – 177Combined sources4
Beta strandi181 – 185Combined sources5
Helixi190 – 198Combined sources9
Beta strandi203 – 207Combined sources5
Helixi208 – 211Combined sources4
Beta strandi213 – 215Combined sources3
Helixi216 – 236Combined sources21

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2HTMX-ray2.30A/B/C/D1-268[»]
ProteinModelPortaliQ5SKG7.
SMRiQ5SKG7.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ5SKG7.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni185 – 186DXP bindingUniRule annotation2
Regioni207 – 208DXP bindingUniRule annotation2

Sequence similaritiesi

Belongs to the ThiG family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CA8. Bacteria.
COG2022. LUCA.
HOGENOMiHOG000248049.
KOiK03149.
OMAiAQYPSPA.
PhylomeDBiQ5SKG7.

Family and domain databases

CDDicd04728. ThiG. 1 hit.
Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00443. ThiG. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR008867. ThiG.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5SKG7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDTWKVGPVE LKSRLILGSG KYEDFGVMRE AIAAAKAEVV TVSVRRVELK
60 70 80 90 100
APGHVGLLEA LEGVRLLPNT AGARTAEEAV RLARLGRLLT GERWVKLEVI
110 120 130 140 150
PDPTYLLPDP LETLKAAERL IEEDFLVLPY MGPDLVLAKR LAALGTATVM
160 170 180 190 200
PLAAPIGSGW GVRTRALLEL FAREKASLPP VVVDAGLGLP SHAAEVMELG
210 220 230 240 250
LDAVLVNTAI AEAQDPPAMA EAFRLAVEAG RKAYLAGPMR PREAASPSSP
260
VEGVPFTPTG PRPGRGPQ
Length:268
Mass (Da):28,449
Last modified:December 21, 2004 - v1
Checksum:iD099F3FB26F4AAB6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP008226 Genomic DNA. Translation: BAD70499.1.
RefSeqiWP_011228115.1. NC_006461.1.
YP_143942.1. NC_006461.1.

Genome annotation databases

EnsemblBacteriaiBAD70499; BAD70499; BAD70499.
GeneIDi3168363.
KEGGittj:TTHA0676.
PATRICi23956329. VBITheThe93045_0670.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP008226 Genomic DNA. Translation: BAD70499.1.
RefSeqiWP_011228115.1. NC_006461.1.
YP_143942.1. NC_006461.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2HTMX-ray2.30A/B/C/D1-268[»]
ProteinModelPortaliQ5SKG7.
SMRiQ5SKG7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi300852.TTHA0676.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAD70499; BAD70499; BAD70499.
GeneIDi3168363.
KEGGittj:TTHA0676.
PATRICi23956329. VBITheThe93045_0670.

Phylogenomic databases

eggNOGiENOG4105CA8. Bacteria.
COG2022. LUCA.
HOGENOMiHOG000248049.
KOiK03149.
OMAiAQYPSPA.
PhylomeDBiQ5SKG7.

Enzyme and pathway databases

UniPathwayiUPA00060.

Miscellaneous databases

EvolutionaryTraceiQ5SKG7.

Family and domain databases

CDDicd04728. ThiG. 1 hit.
Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00443. ThiG. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR008867. ThiG.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTHIG_THET8
AccessioniPrimary (citable) accession number: Q5SKG7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2005
Last sequence update: December 21, 2004
Last modified: November 2, 2016
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.