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Protein

Protein translocase subunit SecDF

Gene

secDF

Organism
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Conducts protons, which can be regulated by a proton gradient or by binding of an unfolded protein. Proton conduction requires the large periplasmic domain of the SecD.
Part of the Sec protein translocase complex. Interacts with the SecYEG preprotein conducting channel. SecDF uses the proton motive force (PMF) to complete protein translocation after the ATP-dependent function of SecA.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Protein transport, Translocation, Transport

Enzyme and pathway databases

BioCyciTTHE300852:GH8R-727-MONOMER.

Protein family/group databases

TCDBi2.A.6.4.3. the resistance-nodulation-cell division (rnd) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein translocase subunit SecDF
Gene namesi
Name:secDF
Ordered Locus Names:TTHA0697
OrganismiThermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Taxonomic identifieri300852 [NCBI]
Taxonomic lineageiBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus
Proteomesi
  • UP000000532 Componenti: Chromosome

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei1 – 2424Helical; Name=1CuratedAdd
BLAST
Topological domaini25 – 268244PeriplasmicCuratedAdd
BLAST
Transmembranei269 – 29325Helical; Name=2CuratedAdd
BLAST
Transmembranei294 – 31522Helical; Name=3CuratedAdd
BLAST
Topological domaini316 – 3249PeriplasmicCurated
Transmembranei325 – 34723Helical; Name=4CuratedAdd
BLAST
Topological domaini348 – 36013CytoplasmicCuratedAdd
BLAST
Transmembranei361 – 39030Helical; Name=5CuratedAdd
BLAST
Topological domaini391 – 3966PeriplasmicCurated
Transmembranei397 – 42529Helical; Name=6CuratedAdd
BLAST
Topological domaini426 – 44419CytoplasmicCuratedAdd
BLAST
Transmembranei445 – 46824Helical; Name=7CuratedAdd
BLAST
Topological domaini469 – 56597PeriplasmicCuratedAdd
BLAST
Transmembranei566 – 58924Helical; Name=8CuratedAdd
BLAST
Transmembranei590 – 61526Helical; Name=9CuratedAdd
BLAST
Topological domaini616 – 6205PeriplasmicCurated
Transmembranei621 – 64626Helical; Name=10CuratedAdd
BLAST
Topological domaini647 – 66014CytoplasmicCuratedAdd
BLAST
Transmembranei661 – 68828Helical; Name=11CuratedAdd
BLAST
Topological domaini689 – 6924PeriplasmicCurated
Transmembranei693 – 72331Helical; Name=12CuratedAdd
BLAST
Topological domaini724 – 73512CytoplasmicCuratedAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi340 – 3401D → N: Abolishes ion channel activity. 1 Publication
Mutagenesisi637 – 6371D → N: No effect on ion channel activity. 1 Publication
Mutagenesisi671 – 6711R → M: Abolishes ion channel activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 735735Protein translocase subunit SecDFPRO_0000412713Add
BLAST

Interactioni

Subunit structurei

Part of the essential Sec protein translocation apparatus which comprises SecA, SecYEG and auxiliary protein SecDF. Other proteins may also be involved (By similarity). Monomer.By similarity1 Publication

Protein-protein interaction databases

STRINGi300852.TTHA0697.

Structurei

Secondary structure

1
735
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi3 – 2321Combined sources
Helixi29 – 324Combined sources
Turni38 – 403Combined sources
Beta strandi43 – 497Combined sources
Beta strandi51 – 533Combined sources
Helixi56 – 7318Combined sources
Beta strandi79 – 835Combined sources
Turni84 – 863Combined sources
Beta strandi87 – 926Combined sources
Helixi97 – 10812Combined sources
Beta strandi113 – 1186Combined sources
Helixi127 – 1359Combined sources
Helixi142 – 1465Combined sources
Helixi152 – 1543Combined sources
Beta strandi159 – 1613Combined sources
Helixi162 – 1643Combined sources
Beta strandi165 – 1728Combined sources
Turni174 – 1763Combined sources
Beta strandi178 – 1847Combined sources
Helixi186 – 19813Combined sources
Turni199 – 2013Combined sources
Beta strandi202 – 2087Combined sources
Beta strandi211 – 2144Combined sources
Beta strandi224 – 2296Combined sources
Helixi235 – 24713Combined sources
Beta strandi254 – 2618Combined sources
Helixi264 – 29330Combined sources
Helixi295 – 31925Combined sources
Helixi325 – 35329Combined sources
Turni354 – 3563Combined sources
Helixi359 – 39032Combined sources
Helixi395 – 42834Combined sources
Turni444 – 4474Combined sources
Helixi448 – 46922Combined sources
Helixi475 – 4784Combined sources
Beta strandi480 – 4878Combined sources
Helixi493 – 5019Combined sources
Turni502 – 5043Combined sources
Beta strandi507 – 5093Combined sources
Beta strandi511 – 5155Combined sources
Beta strandi523 – 5308Combined sources
Helixi534 – 54714Combined sources
Beta strandi548 – 5503Combined sources
Beta strandi554 – 5585Combined sources
Helixi560 – 58829Combined sources
Helixi591 – 61525Combined sources
Helixi621 – 65131Combined sources
Beta strandi653 – 6553Combined sources
Helixi657 – 68933Combined sources
Helixi692 – 72534Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2RRNNMR-A470-559[»]
3AQOX-ray2.60A/B/C/D35-263[»]
3AQPX-ray3.30A/B1-735[»]
ProteinModelPortaliQ5SKE6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 434434SecDAdd
BLAST
Regioni112 – 248137Required for proton transportAdd
BLAST
Regioni435 – 735301SecFAdd
BLAST

Sequence similaritiesi

In the N-terminal section; belongs to the SecD/SecF family. SecD subfamily.Curated
In the C-terminal section; belongs to the SecD/SecF family. SecF subfamily.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105C3D. Bacteria.
COG0341. LUCA.
COG0342. LUCA.
HOGENOMiHOG000018636.
KOiK12257.
OMAiLLLGNWW.
PhylomeDBiQ5SKE6.

Family and domain databases

HAMAPiMF_01463_B. SecD_B. 1 hit.
MF_01464_B. SecF_B. 1 hit.
InterProiIPR005791. SecD.
IPR022813. SecD/SecF_arch_bac.
IPR022645. SecD/SecF_bac.
IPR022646. SecD/SecF_CS.
IPR005665. SecF_bac.
IPR000731. SSD.
[Graphical view]
PfamiPF07549. Sec_GG. 2 hits.
PF02355. SecD_SecF. 2 hits.
[Graphical view]
PRINTSiPR01755. SECFTRNLCASE.
TIGRFAMsiTIGR00916. 2A0604s01. 2 hits.
TIGR00966. 3a0501s07. 1 hit.
TIGR01129. secD. 1 hit.

Sequencei

Sequence statusi: Complete.

Q5SKE6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNRKNLTSLF LLGVFLLALL FVWKPWAPEE PKVRLGLDLK GGLRIVLEAD
60 70 80 90 100
VENPTLDDLE KARTVLENRI NALGVAEPLI QIQGQKRIVV ELPGLSQADQ
110 120 130 140 150
DRALKLIGQR AVLEFRIVKE GATGTTVAQI NQALRENPRL NREELEKDLI
160 170 180 190 200
KPEDLGPPLL TGADLADARA VFDQFGRPQV SLTFTPEGAK KFEEVTRQNI
210 220 230 240 250
GKRLAIVLDG RVYTAPVIRQ AITGGQAVIE GLSSVEEASE IALVLRSGSL
260 270 280 290 300
PVPLKVAEIR AIGPTLGQDA IQAGIRSALI GTLAIFLLIF AYYGPHLGLV
310 320 330 340 350
ASLGLLYTSA LILGLLSGLG ATLTLPGIAG LVLTLGAAVD GNVLSFERIK
360 370 380 390 400
EELRAGKKLR QAIPEGFRHS TLTIMDVNIA HLLAAAALYQ YATGPVRGFA
410 420 430 440 450
VILAIGVVAS VFSNLVFSRH LLERLADRGE IRPPMWLVDP RFNFMGPARY
460 470 480 490 500
VTAATLLLAA LAAGVVFAKG FNYSIDFTGG TAYTLRAEPN VEVETLRRFL
510 520 530 540 550
EEKGFPGKEA VITQVQAPTA AYREFLVKLP PLSDERRLEL ERLFASELKA
560 570 580 590 600
TVLASETVGP AIGEELRRNA VMAVLVGLGL ILLYVAFRFD WTFGVASILA
610 620 630 640 650
VAHDVAIVAG MYSLLGLEFS IPTIAALLTI VGYSINDSIV VSDRIRENQK
660 670 680 690 700
LLRHLPYAEL VNRSINQTLS RTVMTSLTTL LPILALLFLG GSVLRDFALA
710 720 730
IFVGIFVGTY SSIYVVSALV VAWKNRRKAQ EASKA
Length:735
Mass (Da):79,678
Last modified:December 21, 2004 - v1
Checksum:i6283A10A1163539D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP008226 Genomic DNA. Translation: BAD70520.1.
RefSeqiWP_011172795.1. NC_006461.1.
YP_143963.1. NC_006461.1.

Genome annotation databases

EnsemblBacteriaiBAD70520; BAD70520; BAD70520.
GeneIDi3168575.
KEGGittj:TTHA0697.
PATRICi23956371. VBITheThe93045_0691.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP008226 Genomic DNA. Translation: BAD70520.1.
RefSeqiWP_011172795.1. NC_006461.1.
YP_143963.1. NC_006461.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2RRNNMR-A470-559[»]
3AQOX-ray2.60A/B/C/D35-263[»]
3AQPX-ray3.30A/B1-735[»]
ProteinModelPortaliQ5SKE6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi300852.TTHA0697.

Protein family/group databases

TCDBi2.A.6.4.3. the resistance-nodulation-cell division (rnd) superfamily.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAD70520; BAD70520; BAD70520.
GeneIDi3168575.
KEGGittj:TTHA0697.
PATRICi23956371. VBITheThe93045_0691.

Phylogenomic databases

eggNOGiENOG4105C3D. Bacteria.
COG0341. LUCA.
COG0342. LUCA.
HOGENOMiHOG000018636.
KOiK12257.
OMAiLLLGNWW.
PhylomeDBiQ5SKE6.

Enzyme and pathway databases

BioCyciTTHE300852:GH8R-727-MONOMER.

Family and domain databases

HAMAPiMF_01463_B. SecD_B. 1 hit.
MF_01464_B. SecF_B. 1 hit.
InterProiIPR005791. SecD.
IPR022813. SecD/SecF_arch_bac.
IPR022645. SecD/SecF_bac.
IPR022646. SecD/SecF_CS.
IPR005665. SecF_bac.
IPR000731. SSD.
[Graphical view]
PfamiPF07549. Sec_GG. 2 hits.
PF02355. SecD_SecF. 2 hits.
[Graphical view]
PRINTSiPR01755. SECFTRNLCASE.
TIGRFAMsiTIGR00916. 2A0604s01. 2 hits.
TIGR00966. 3a0501s07. 1 hit.
TIGR01129. secD. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiSECDF_THET8
AccessioniPrimary (citable) accession number: Q5SKE6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 21, 2011
Last sequence update: December 21, 2004
Last modified: September 7, 2016
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.