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Protein

Protein translocase subunit SecDF

Gene

secDF

Organism
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Conducts protons, which can be regulated by a proton gradient or by binding of an unfolded protein. Proton conduction requires the large periplasmic domain of the SecD.
Part of the Sec protein translocase complex. Interacts with the SecYEG preprotein conducting channel. SecDF uses the proton motive force (PMF) to complete protein translocation after the ATP-dependent function of SecA.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processProtein transport, Translocation, Transport

Enzyme and pathway databases

BioCyciTTHE300852:G1GKC-714-MONOMER

Protein family/group databases

TCDBi2.A.6.4.3 the resistance-nodulation-cell division (rnd) superfamily

Names & Taxonomyi

Protein namesi
Recommended name:
Protein translocase subunit SecDF
Gene namesi
Name:secDF
Ordered Locus Names:TTHA0697
OrganismiThermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Taxonomic identifieri300852 [NCBI]
Taxonomic lineageiBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus
Proteomesi
  • UP000000532 Componenti: Chromosome

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei1 – 24Helical; Name=1CuratedAdd BLAST24
Topological domaini25 – 268PeriplasmicCuratedAdd BLAST244
Transmembranei269 – 293Helical; Name=2CuratedAdd BLAST25
Transmembranei294 – 315Helical; Name=3CuratedAdd BLAST22
Topological domaini316 – 324PeriplasmicCurated9
Transmembranei325 – 347Helical; Name=4CuratedAdd BLAST23
Topological domaini348 – 360CytoplasmicCuratedAdd BLAST13
Transmembranei361 – 390Helical; Name=5CuratedAdd BLAST30
Topological domaini391 – 396PeriplasmicCurated6
Transmembranei397 – 425Helical; Name=6CuratedAdd BLAST29
Topological domaini426 – 444CytoplasmicCuratedAdd BLAST19
Transmembranei445 – 468Helical; Name=7CuratedAdd BLAST24
Topological domaini469 – 565PeriplasmicCuratedAdd BLAST97
Transmembranei566 – 589Helical; Name=8CuratedAdd BLAST24
Transmembranei590 – 615Helical; Name=9CuratedAdd BLAST26
Topological domaini616 – 620PeriplasmicCurated5
Transmembranei621 – 646Helical; Name=10CuratedAdd BLAST26
Topological domaini647 – 660CytoplasmicCuratedAdd BLAST14
Transmembranei661 – 688Helical; Name=11CuratedAdd BLAST28
Topological domaini689 – 692PeriplasmicCurated4
Transmembranei693 – 723Helical; Name=12CuratedAdd BLAST31
Topological domaini724 – 735CytoplasmicCuratedAdd BLAST12

GO - Cellular componenti

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi340D → N: Abolishes ion channel activity. 1 Publication1
Mutagenesisi637D → N: No effect on ion channel activity. 1 Publication1
Mutagenesisi671R → M: Abolishes ion channel activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004127131 – 735Protein translocase subunit SecDFAdd BLAST735

Interactioni

Subunit structurei

Part of the essential Sec protein translocation apparatus which comprises SecA, SecYEG and auxiliary protein SecDF. Other proteins may also be involved (By similarity). Monomer.By similarity1 Publication

Protein-protein interaction databases

STRINGi300852.TTHA0697

Structurei

Secondary structure

1735
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi4 – 23Combined sources20
Beta strandi32 – 35Combined sources4
Turni37 – 40Combined sources4
Beta strandi43 – 49Combined sources7
Beta strandi51 – 53Combined sources3
Helixi56 – 73Combined sources18
Beta strandi79 – 83Combined sources5
Turni84 – 86Combined sources3
Beta strandi87 – 92Combined sources6
Helixi97 – 108Combined sources12
Beta strandi113 – 118Combined sources6
Helixi127 – 135Combined sources9
Helixi142 – 146Combined sources5
Helixi152 – 154Combined sources3
Beta strandi159 – 161Combined sources3
Helixi162 – 164Combined sources3
Beta strandi165 – 172Combined sources8
Beta strandi174 – 176Combined sources3
Beta strandi178 – 184Combined sources7
Helixi186 – 198Combined sources13
Turni199 – 201Combined sources3
Beta strandi202 – 208Combined sources7
Beta strandi211 – 214Combined sources4
Beta strandi224 – 229Combined sources6
Helixi235 – 247Combined sources13
Beta strandi254 – 261Combined sources8
Helixi268 – 319Combined sources52
Helixi325 – 354Combined sources30
Helixi359 – 390Combined sources32
Helixi395 – 415Combined sources21
Helixi417 – 427Combined sources11
Turni445 – 447Combined sources3
Helixi448 – 469Combined sources22
Helixi475 – 478Combined sources4
Beta strandi480 – 487Combined sources8
Helixi493 – 502Combined sources10
Turni507 – 509Combined sources3
Beta strandi510 – 515Combined sources6
Beta strandi523 – 529Combined sources7
Helixi534 – 546Combined sources13
Beta strandi548 – 550Combined sources3
Beta strandi551 – 558Combined sources8
Helixi564 – 588Combined sources25
Helixi591 – 615Combined sources25
Beta strandi618 – 620Combined sources3
Helixi621 – 651Combined sources31
Turni652 – 654Combined sources3
Helixi657 – 689Combined sources33
Turni692 – 694Combined sources3
Helixi695 – 714Combined sources20
Helixi716 – 734Combined sources19

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2RRNNMR-A470-559[»]
3AQOX-ray2.60A/B/C/D35-263[»]
3AQPX-ray3.30A/B1-735[»]
5YHFX-ray2.80A1-735[»]
ProteinModelPortaliQ5SKE6
SMRiQ5SKE6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 434SecDAdd BLAST434
Regioni112 – 248Required for proton transportAdd BLAST137
Regioni435 – 735SecFAdd BLAST301

Sequence similaritiesi

In the N-terminal section; belongs to the SecD/SecF family. SecD subfamily.Curated
In the C-terminal section; belongs to the SecD/SecF family. SecF subfamily.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105C3D Bacteria
COG0341 LUCA
COG0342 LUCA
HOGENOMiHOG000018636
KOiK12257
OMAiPNNQLVI
PhylomeDBiQ5SKE6

Family and domain databases

HAMAPiMF_01463_B SecD_B, 1 hit
MF_01464_B SecF_B, 1 hit
InterProiView protein in InterPro
IPR005791 SecD
IPR022813 SecD/SecF_arch_bac
IPR022645 SecD/SecF_bac
IPR022646 SecD/SecF_CS
IPR005665 SecF_bac
IPR000731 SSD
PfamiView protein in Pfam
PF07549 Sec_GG, 2 hits
PF02355 SecD_SecF, 2 hits
PRINTSiPR01755 SECFTRNLCASE
TIGRFAMsiTIGR00916 2A0604s01, 2 hits
TIGR00966 3a0501s07, 1 hit
TIGR01129 secD, 1 hit

Sequencei

Sequence statusi: Complete.

Q5SKE6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNRKNLTSLF LLGVFLLALL FVWKPWAPEE PKVRLGLDLK GGLRIVLEAD
60 70 80 90 100
VENPTLDDLE KARTVLENRI NALGVAEPLI QIQGQKRIVV ELPGLSQADQ
110 120 130 140 150
DRALKLIGQR AVLEFRIVKE GATGTTVAQI NQALRENPRL NREELEKDLI
160 170 180 190 200
KPEDLGPPLL TGADLADARA VFDQFGRPQV SLTFTPEGAK KFEEVTRQNI
210 220 230 240 250
GKRLAIVLDG RVYTAPVIRQ AITGGQAVIE GLSSVEEASE IALVLRSGSL
260 270 280 290 300
PVPLKVAEIR AIGPTLGQDA IQAGIRSALI GTLAIFLLIF AYYGPHLGLV
310 320 330 340 350
ASLGLLYTSA LILGLLSGLG ATLTLPGIAG LVLTLGAAVD GNVLSFERIK
360 370 380 390 400
EELRAGKKLR QAIPEGFRHS TLTIMDVNIA HLLAAAALYQ YATGPVRGFA
410 420 430 440 450
VILAIGVVAS VFSNLVFSRH LLERLADRGE IRPPMWLVDP RFNFMGPARY
460 470 480 490 500
VTAATLLLAA LAAGVVFAKG FNYSIDFTGG TAYTLRAEPN VEVETLRRFL
510 520 530 540 550
EEKGFPGKEA VITQVQAPTA AYREFLVKLP PLSDERRLEL ERLFASELKA
560 570 580 590 600
TVLASETVGP AIGEELRRNA VMAVLVGLGL ILLYVAFRFD WTFGVASILA
610 620 630 640 650
VAHDVAIVAG MYSLLGLEFS IPTIAALLTI VGYSINDSIV VSDRIRENQK
660 670 680 690 700
LLRHLPYAEL VNRSINQTLS RTVMTSLTTL LPILALLFLG GSVLRDFALA
710 720 730
IFVGIFVGTY SSIYVVSALV VAWKNRRKAQ EASKA
Length:735
Mass (Da):79,678
Last modified:December 21, 2004 - v1
Checksum:i6283A10A1163539D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP008226 Genomic DNA Translation: BAD70520.1
RefSeqiWP_011172795.1, NC_006461.1
YP_143963.1, NC_006461.1

Genome annotation databases

EnsemblBacteriaiBAD70520; BAD70520; BAD70520
GeneIDi3168575
KEGGittj:TTHA0697
PATRICifig|300852.9.peg.691

Similar proteinsi

Entry informationi

Entry nameiSECDF_THET8
AccessioniPrimary (citable) accession number: Q5SKE6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 21, 2011
Last sequence update: December 21, 2004
Last modified: April 25, 2018
This is version 82 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
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Main funding by: National Institutes of Health