Dihydroorotase - Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)

Preferred order of sections

Names and origin

Protein names

Recommended name:
Dihydroorotase RuleBase RU003441
EC=3.5.2.3 RuleBase RU003441

Gene names

Ordered Locus Names:

TTHA0781

Organism

Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)

Taxonomic identifier

300852 [NCBI]

Taxonomic lineage

Bacteria › Deinococcus-Thermus › Deinococci › Thermales › Thermaceae › Thermus

Protein attributes

Sequence length	426 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	(S)-dihydroorotate + H₂O = N-carbamoyl-L-aspartate. RuleBase RU003441 SAAS SAAS002195
Cofactor	Binds 2 zinc ions per subunit By similarity. RuleBase RU003441 SAAS SAAS002195
Pathway	Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 3/3. RuleBase RU003441 SAAS SAAS002195
Subunit structure	Homodimer By similarity. SAAS SAAS002195
Sequence similarities	Belongs to the DHOase family. Type 2 subfamily. RuleBase RU003441

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis RuleBase RU003441 SAAS SAAS002195
Ligand	Metal-binding RuleBase RU003441 SAAS SAAS002195 PDB 2Z00 Zinc SAAS SAAS002195 RuleBase RU003441 PDB 2Z00
Molecular function	Hydrolase RuleBase RU003441 SAAS SAAS002195
Technical term	3D-structure PDB 2Z00 Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	pyrimidine nucleotide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	dihydroorotase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Sites

Metal binding

55

1

Zinc 1; via tele nitrogen PDB 2Z00

Metal binding

57

1

Zinc 1; via tele nitrogen PDB 2Z00

Metal binding

147

1

Zinc 1 PDB 2Z00

Metal binding

147

1

Zinc 2 PDB 2Z00

Metal binding

174

1

Zinc 2; via pros nitrogen PDB 2Z00

Metal binding

233

1

Zinc 2; via tele nitrogen PDB 2Z00

Metal binding

306

1

Zinc 1 PDB 2Z00

Sequences

Sequence

Length

Mass (Da)

Tools

Q5SK67 [UniParc].

Last modified December 21, 2004. Version 1.
Checksum: 753EBA4721371781
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FASTA

426

45,694

        10         20         30         40         50         60 
MILIRNVRLV DARGERGPAD VLIGEGRILS LEGGEAKQVV DGTGCFLAPG FLDLHAHLRE 

        70         80         90        100        110        120 
PGEEVKEDLF SGLLAAVRGG YTDLVSMPNT KPPVDTPEAV RALKEKAKAL GLARLHPAAA 

       130        140        150        160        170        180 
LTEKQEGKTL TPAGLLREAG AVLLTDDGRT NEDAGVLAAG LLMAAPLGLP VAVHAEDAGL 

       190        200        210        220        230        240 
RRNGVMNDGP LADLLGLPGN PPEAEAARIA RDLEVLRYAL RRSPATPRLH VQHLSTKRGL 

       250        260        270        280        290        300 
ELVREAKRAG LPVTAEATPH HLTLTEEALR TFDPLFKVAP PLRGEEDREA LLEGLLDGTL 

       310        320        330        340        350        360 
DAIATDHAPH TLAEKEKDLL RAPFGIPSLE VAFPLLYTEL HLKRGFPLQR LVELFTDGPR 

       370        380        390        400        410        420 
RVLGLPPLHL EEGAEASLVL LSPKERPVDP SAFASKARYS PWAGWVLGGW PVLTLVAGRI 


VHEALK

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete genome sequence of Thermus thermophilus HB8." Masui R., Kurokawa K., Nakagawa N., Tokunaga F., Koyama Y., Shibata T., Oshima T., Yokoyama S., Yasunaga T., Kuramitsu S. Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: HB8 / ATCC 27634 / DSM 579.
[2]	"Crystal structure of dihydroorotase from Thermus thermophilus." Kanagawa M., Baba S., Kuramitsu S., Yokoyama S., Kawai G., Sampei G. Submitted (MAY-2007) to the PDB data bank Cited for: X-RAY CRYSTALLOGRAPHY (2.42 ANGSTROMS) IN COMPLEX WITH ZINC.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AP008226 Genomic DNA. Translation: BAD70604.1.

RefSeq

YP_144047.1. NC_006461.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2Z00	X-ray	2.42	A	1-426	[»]

ProteinModelPortal

Q5SK67.

SMR

Q5SK67. Positions 1-426.

ModBase

Search...

Protein-protein interaction databases

STRING

Q5SK67.

Protein family/group databases

MEROPS

M38.972.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

GeneID

3170046.

GenomeReviews

Gene locus TTHA0781 in contig AP008226_GR.

KEGG

ttj:TTHA0781.

NMPDR

fig|300852.3.peg.811.

PATRIC

23956539. VBITheThe93045_0774.

Phylogenomic databases

eggNOG

COG0044.

HOGENOM

HBG724623.

OMA

CDVHPVG.

PhylomeDB

Q5SK67.

ProtClustDB

PRK09357.

Enzyme and pathway databases

BioCyc

TTHE300852:TTHA0781-MONOMER.

Family and domain databases

HAMAP

MF_00220_B. PyrC_type2_B.
[Tree]

InterPro

IPR006680. Amidohydro_1.
IPR004722. DHOase.
IPR002195. Dihydroorotase_CS.
IPR011059. Metal-dep_hydrolase_composite.
[Graphical view]

KO

K01465.

Pfam

PF01979. Amidohydro_1. 1 hit.
[Graphical view]

SUPFAM

SSF51338. Metalo_hydrolase. 1 hit.

TIGRFAMs

TIGR00857. PyrC_multi. 1 hit.

PROSITE

PS00482. DIHYDROOROTASE_1. 1 hit.
PS00483. DIHYDROOROTASE_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

Q5SK67_THET8

Accession

Primary (citable) accession number: Q5SK67

Entry history

Integrated into UniProtKB/TrEMBL:	December 21, 2004
Last sequence update:	December 21, 2004
Last modified:	December 14, 2011
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)