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Protein

Ribosomal RNA small subunit methyltransferase B

Gene

rsmB

Organism
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Specifically methylates the cytosine at position 967 (m5C967) of 16S rRNA.1 Publication

Catalytic activityi

S-adenosyl-L-methionine + cytosine(967) in 16S rRNA = S-adenosyl-L-homocysteine + 5-methylcytosine(967) in 16S rRNA.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei242S-adenosyl-L-methionineBy similarity1
Binding sitei268S-adenosyl-L-methionineBy similarity1
Binding sitei283S-adenosyl-L-methionineBy similarity1
Active sitei336NucleophilePROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

rRNA processing

Keywords - Ligandi

RNA-binding, S-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
Ribosomal RNA small subunit methyltransferase BBy similarity (EC:2.1.1.1761 Publication)
Alternative name(s):
16S rRNA m5C967 methyltransferaseBy similarity
rRNA (cytosine-C(5)-)-methyltransferase RsmBBy similarity
Gene namesi
Name:rsmB1 Publication
Ordered Locus Names:TTHA0851Imported
OrganismiThermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Taxonomic identifieri300852 [NCBI]
Taxonomic lineageiBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus
Proteomesi
  • UP000000532 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004314801 – 398Ribosomal RNA small subunit methyltransferase BAdd BLAST398

Interactioni

Protein-protein interaction databases

STRINGi300852.TTHA0851.

Structurei

3D structure databases

ProteinModelPortaliQ5SK01.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni221 – 227S-adenosyl-L-methionine bindingBy similarity7

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. RsmB/NOP family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG4105CYJ. Bacteria.
COG0144. LUCA.
HOGENOMiHOG000037302.
KOiK03500.
OMAiPREAPEC.
PhylomeDBiQ5SK01.

Family and domain databases

Gene3Di1.10.940.10. 1 hit.
3.40.50.150. 1 hit.
InterProiIPR001678. MeTrfase_RsmB/NOP2.
IPR006027. NusB_RsmB_TIM44.
IPR023267. RCMT.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF01189. Methyltr_RsmB-F. 1 hit.
PF01029. NusB. 1 hit.
[Graphical view]
PRINTSiPR02008. RCMTFAMILY.
SUPFAMiSSF48013. SSF48013. 1 hit.
SSF53335. SSF53335. 1 hit.
PROSITEiPS51686. SAM_MT_RSMB_NOP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5SK01-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRAGTPRALA VAVLLEVDRG GRAQLLLDRA LRRSPWPERD RAYATFLVYG
60 70 80 90 100
ALRRLRLLDH LLAPLLPRPE GLPPEVRWIL RLGALEWLEG KPDHARVSPW
110 120 130 140 150
VEEAKRRYPG LAGLVNAVLR RLAPREAPEC VRLSLPDWLC EAWRGFFGDV
160 170 180 190 200
AFAEGFNEPA PLFVTAYREV DLRPGPVPGS YLWEGPKTDF SALGLQPQNP
210 220 230 240 250
ASLFAAKLLE ARPGERVLDL CGGAGLKAFY LAAQGAEVVS YDLNRRRQEA
260 270 280 290 300
GARTARRLGL WVHYRTQDLT RPVPERAKKV LLDAPCTGTG TFRAHPELRY
310 320 330 340 350
RLSPEDPARM AALQLQLLET AAQATEEGGV LVYSVCTLTE EEGEGVVRAF
360 370 380 390
LARHPEFRPE PVQPPFPVLA RGLGVYVDPR GGLDGFYYAK LRRVNSQA
Length:398
Mass (Da):44,312
Last modified:December 21, 2004 - v1
Checksum:i60BD5FB94549F335
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP008226 Genomic DNA. Translation: BAD70674.1.
RefSeqiWP_011228244.1. NC_006461.1.
YP_144117.1. NC_006461.1.

Genome annotation databases

EnsemblBacteriaiBAD70674; BAD70674; BAD70674.
GeneIDi3170008.
KEGGittj:TTHA0851.
PATRICi23956684. VBITheThe93045_0845.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP008226 Genomic DNA. Translation: BAD70674.1.
RefSeqiWP_011228244.1. NC_006461.1.
YP_144117.1. NC_006461.1.

3D structure databases

ProteinModelPortaliQ5SK01.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi300852.TTHA0851.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAD70674; BAD70674; BAD70674.
GeneIDi3170008.
KEGGittj:TTHA0851.
PATRICi23956684. VBITheThe93045_0845.

Phylogenomic databases

eggNOGiENOG4105CYJ. Bacteria.
COG0144. LUCA.
HOGENOMiHOG000037302.
KOiK03500.
OMAiPREAPEC.
PhylomeDBiQ5SK01.

Family and domain databases

Gene3Di1.10.940.10. 1 hit.
3.40.50.150. 1 hit.
InterProiIPR001678. MeTrfase_RsmB/NOP2.
IPR006027. NusB_RsmB_TIM44.
IPR023267. RCMT.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF01189. Methyltr_RsmB-F. 1 hit.
PF01029. NusB. 1 hit.
[Graphical view]
PRINTSiPR02008. RCMTFAMILY.
SUPFAMiSSF48013. SSF48013. 1 hit.
SSF53335. SSF53335. 1 hit.
PROSITEiPS51686. SAM_MT_RSMB_NOP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRSMB_THET8
AccessioniPrimary (citable) accession number: Q5SK01
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 7, 2015
Last sequence update: December 21, 2004
Last modified: November 2, 2016
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.