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Protein

4-hydroxyphenylacetate 3-monooxygenase oxygenase component

Gene

TTHA0960

Organism
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Utilizes FADH2 supplied by HpaC, to catalyze the hydroxylation of 4-hydroxyphenylacetic acid, leading to the production of 3,4-dihydroxyphenylacetic acid (DHPA).1 Publication

Catalytic activityi

4-hydroxyphenylacetate + FADH2 + O2 = 3,4-dihydroxyphenylacetate + FAD + H2O.1 Publication

Pathwayi: 4-hydroxyphenylacetate degradation

This protein is involved in step 1 of the subpathway that synthesizes pyruvate and succinate semialdehyde from 4-hydroxyphenylacetate.
Proteins known to be involved in the 7 steps of the subpathway in this organism are:
  1. 4-hydroxyphenylacetate 3-monooxygenase reductase component (TTHA0961), 4-hydroxyphenylacetate 3-monooxygenase oxygenase component (TTHA0960)
  2. no protein annotated in this organism
  3. no protein annotated in this organism
  4. no protein annotated in this organism
  5. no protein annotated in this organism
  6. no protein annotated in this organism
  7. no protein annotated in this organism
This subpathway is part of the pathway 4-hydroxyphenylacetate degradation, which is itself part of Aromatic compound metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate and succinate semialdehyde from 4-hydroxyphenylacetate, the pathway 4-hydroxyphenylacetate degradation and in Aromatic compound metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei142Substrate1 Publication1
Binding sitei185FAD1 Publication1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi142 – 144FAD1 Publication3
Nucleotide bindingi148 – 151FAD1 Publication4
Nucleotide bindingi444 – 447FAD1 Publication4

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Biological processi

Aromatic hydrocarbons catabolism

Keywords - Ligandi

FAD, Flavoprotein

Enzyme and pathway databases

BioCyciTTHE300852:GH8R-962-MONOMER.
BRENDAi1.14.14.9. 2305.
UniPathwayiUPA00208; UER00416.

Names & Taxonomyi

Protein namesi
Recommended name:
4-hydroxyphenylacetate 3-monooxygenase oxygenase component (EC:1.14.14.9)
Alternative name(s):
4-HPA 3-hydroxylase
4-HPA 3-monooxygenase large component
Gene namesi
Ordered Locus Names:TTHA0960
OrganismiThermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Taxonomic identifieri300852 [NCBI]
Taxonomic lineageiBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus
Proteomesi
  • UP000000532 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003879941 – 4814-hydroxyphenylacetate 3-monooxygenase oxygenase componentAdd BLAST481

Interactioni

Subunit structurei

Homotetramer consisting of a dimer of dimers. 4-HPA 3-monooxygenase consists of a reductase component HpaC and an oxygenase component HpaB.1 Publication

Protein-protein interaction databases

STRINGi300852.TTHA0960.

Structurei

Secondary structure

1481
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi5 – 14Combined sources10
Beta strandi19 – 21Combined sources3
Turni29 – 31Combined sources3
Turni33 – 35Combined sources3
Helixi36 – 48Combined sources13
Turni52 – 54Combined sources3
Helixi55 – 58Combined sources4
Beta strandi59 – 62Combined sources4
Beta strandi65 – 68Combined sources4
Helixi69 – 71Combined sources3
Helixi77 – 93Combined sources17
Turni94 – 96Combined sources3
Helixi103 – 114Combined sources12
Helixi116 – 122Combined sources7
Helixi123 – 136Combined sources14
Beta strandi140 – 144Combined sources5
Helixi155 – 157Combined sources3
Beta strandi158 – 160Combined sources3
Beta strandi166 – 170Combined sources5
Beta strandi172 – 185Combined sources14
Beta strandi189 – 194Combined sources6
Beta strandi198 – 200Combined sources3
Helixi205 – 207Combined sources3
Beta strandi209 – 214Combined sources6
Beta strandi220 – 224Combined sources5
Turni234 – 236Combined sources3
Turni238 – 242Combined sources5
Beta strandi247 – 258Combined sources12
Helixi259 – 261Combined sources3
Beta strandi262 – 266Combined sources5
Helixi268 – 278Combined sources11
Helixi280 – 309Combined sources30
Helixi312 – 314Combined sources3
Helixi316 – 341Combined sources26
Helixi355 – 380Combined sources26
Helixi381 – 384Combined sources4
Helixi390 – 394Combined sources5
Helixi398 – 404Combined sources7
Beta strandi408 – 410Combined sources3
Helixi412 – 424Combined sources13
Turni425 – 427Combined sources3
Helixi429 – 440Combined sources12
Helixi445 – 455Combined sources11
Helixi459 – 468Combined sources10
Helixi470 – 473Combined sources4
Helixi474 – 476Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2YYGX-ray2.00A1-481[»]
2YYIX-ray1.66A1-481[»]
2YYJX-ray1.66A1-481[»]
2YYKX-ray1.60A1-481[»]
2YYLX-ray1.75A1-481[»]
2YYMX-ray1.70A1-481[»]
ProteinModelPortaliQ5SJP8.
SMRiQ5SJP8.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ5SJP8.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni100 – 104Substrate binding5
Regioni197 – 198Substrate binding2

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105EFA. Bacteria.
COG2368. LUCA.
HOGENOMiHOG000145842.
KOiK00483.
OMAiRIWEINS.
PhylomeDBiQ5SJP8.

Family and domain databases

InterProiIPR009075. AcylCo_DH/oxidase_C.
IPR009100. AcylCoA_DH/oxidase_NM_dom.
IPR004925. HpaB/PvcC/4-BUDH.
IPR024719. HpaB/PvcC/4-BUDH_C.
IPR024674. HpaB/PvcC/4-BUDH_N.
IPR012687. HpaB_Deino-type.
[Graphical view]
PfamiPF03241. HpaB. 1 hit.
PF11794. HpaB_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000331. HpaA_HpaB. 1 hit.
SUPFAMiSSF47203. SSF47203. 1 hit.
SSF56645. SSF56645. 1 hit.
TIGRFAMsiTIGR02309. HpaB-1. 1 hit.

Sequencei

Sequence statusi: Complete.

Q5SJP8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MARTGAEYIE ALKTRPPNLW YKGEKVEDPT THPVFRGIVR TMAALYDLQH
60 70 80 90 100
DPRYREVLTY EEEGKRHGMS FLIPKTKEDL KRRGQAYKLW ADQNLGMMGR
110 120 130 140 150
SPDYLNAVVM AYAASADYFG EFAENVRNYY RYLRDQDLAT THALTNPQVN
160 170 180 190 200
RARPPSGQPD PYIPVGVVKQ TEKGIVVRGA RMTATFPLAD EVLIFPSTLL
210 220 230 240 250
QAGSEKYALA FALPTSTPGL HFVCREALVG GDSPFDHPLS SRVEEMDCLV
260 270 280 290 300
IFDDVLVPWE RVFILGNVEL CNNAYAATGA LNHMAHQVVA LKTAKTEAFL
310 320 330 340 350
GVAALMAEGI GADVYGHVQE KIAEIIVYLE AMRAFWTRAE EEAKENAYGL
360 370 380 390 400
LVPDRGALDG ARNLYPRLYP RIREILEQIG ASGLITLPSE KDFKGPLGPF
410 420 430 440 450
LEKFLQGAAL EAKERVALFR LAWDMTLSGF GARQELYERF FFGDPVRMYQ
460 470 480
TLYNVYNKEP YKERIRAFLK ESLKVFEEVQ A
Length:481
Mass (Da):54,303
Last modified:December 21, 2004 - v1
Checksum:i29ED75B3C034FBC0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP008226 Genomic DNA. Translation: BAD70783.1.
RefSeqiWP_011228328.1. NC_006461.1.
YP_144226.1. NC_006461.1.

Genome annotation databases

EnsemblBacteriaiBAD70783; BAD70783; BAD70783.
GeneIDi3170066.
KEGGittj:TTHA0960.
PATRICi23956882. VBITheThe93045_0942.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP008226 Genomic DNA. Translation: BAD70783.1.
RefSeqiWP_011228328.1. NC_006461.1.
YP_144226.1. NC_006461.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2YYGX-ray2.00A1-481[»]
2YYIX-ray1.66A1-481[»]
2YYJX-ray1.66A1-481[»]
2YYKX-ray1.60A1-481[»]
2YYLX-ray1.75A1-481[»]
2YYMX-ray1.70A1-481[»]
ProteinModelPortaliQ5SJP8.
SMRiQ5SJP8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi300852.TTHA0960.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAD70783; BAD70783; BAD70783.
GeneIDi3170066.
KEGGittj:TTHA0960.
PATRICi23956882. VBITheThe93045_0942.

Phylogenomic databases

eggNOGiENOG4105EFA. Bacteria.
COG2368. LUCA.
HOGENOMiHOG000145842.
KOiK00483.
OMAiRIWEINS.
PhylomeDBiQ5SJP8.

Enzyme and pathway databases

UniPathwayiUPA00208; UER00416.
BioCyciTTHE300852:GH8R-962-MONOMER.
BRENDAi1.14.14.9. 2305.

Miscellaneous databases

EvolutionaryTraceiQ5SJP8.

Family and domain databases

InterProiIPR009075. AcylCo_DH/oxidase_C.
IPR009100. AcylCoA_DH/oxidase_NM_dom.
IPR004925. HpaB/PvcC/4-BUDH.
IPR024719. HpaB/PvcC/4-BUDH_C.
IPR024674. HpaB/PvcC/4-BUDH_N.
IPR012687. HpaB_Deino-type.
[Graphical view]
PfamiPF03241. HpaB. 1 hit.
PF11794. HpaB_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000331. HpaA_HpaB. 1 hit.
SUPFAMiSSF47203. SSF47203. 1 hit.
SSF56645. SSF56645. 1 hit.
TIGRFAMsiTIGR02309. HpaB-1. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiHPAB_THET8
AccessioniPrimary (citable) accession number: Q5SJP8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 3, 2009
Last sequence update: December 21, 2004
Last modified: November 2, 2016
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.