Q5SJG6 (Q5SJG6_THET8) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Submitted name:
Anti-cleavage anti-GreA transcription factor Gfh1 EMBL BAD70865.1

Gene names

Ordered Locus Names:

TTHA1042

Organism

Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)

Taxonomic identifier

300852 [NCBI]

Taxonomic lineage

Bacteria › Deinococcus-Thermus › Deinococci › Thermales › Thermaceae › Thermus

Protein attributes

Sequence length	156 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function

Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by cleavage factors such as greA or greB allows the resumption of elongation from the new 3'terminus. GreA releases sequences of 2 to 3 nucleotides By similarity. SAAS SAAS018151

Ontologies

Keywords
Biological process	Transcription Transcription regulation SAAS SAAS001437
Ligand	DNA-binding SAAS SAAS001437
Technical term	3D-structure PDB 2EUL PDB 3AOI PDB 3AOH Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	regulation of transcription elongation, DNA-dependent Inferred from electronic annotation. Source: InterPro transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	Q5SJG6 [UniParc]. Last modified December 21, 2004. Version 1. Checksum: 2B7BB05B0E8ECE14	FASTA	156	17,183
	10 20 30 40 50 60 MAREVKLTKA GYERLMQQLE RERERLQEAT KILQELMESS DDYDDSGLEA AKQEKARIEA 70 80 90 100 110 120 RIDSLEDILS RAVILEEGSG EVIGLGSVVE LEDPLSGERL SVQVVSPAEA NVLDTPMKIS 130 140 150 DASPMGKALL GHRVGDVLSL DTPKGRREFR VVAIHG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete genome sequence of Thermus thermophilus HB8." Masui R., Kurokawa K., Nakagawa N., Tokunaga F., Koyama Y., Shibata T., Oshima T., Yokoyama S., Yasunaga T., Kuramitsu S. Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: HB8 / ATCC 27634 / DSM 579.
[2]	"Regulation through the RNA polymerase secondary channel. Structural and functional variability of the coiled-coil transcription factors." Symersky J., Perederina A., Vassylyeva M.N., Svetlov V., Artsimovitch I., Vassylyev D.G. J. Biol. Chem. 281:1309-1312(2006) [PubMed: 16298991] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.40 ANGSTROMS).
[3]	"Crystal structure of bacterial RNA polymerase bound with a transcription inhibitor protein." Tagami S., Sekine S., Kumarevel T., Hino N., Murayama Y., Kamegamori S., Yamamoto M., Sakamoto K., Yokoyama S. Nature 468:978-982(2010) [PubMed: 21124318] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (4.10 ANGSTROMS).

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AP008226 Genomic DNA. Translation: BAD70865.1.

RefSeq

YP_144308.1. NC_006461.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2EUL	X-ray	2.40	A/B/C/D	1-156	[»]
3AOH	X-ray	4.10	X/Y/Z	1-156	[»]
3AOI	X-ray	4.30	X/Y/Z	1-156	[»]

ProteinModelPortal

Q5SJG6.

SMR

Q5SJG6. Positions 1-156.

ModBase

Search...

Protein-protein interaction databases

STRING

Q5SJG6.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

GeneID

3168268.

GenomeReviews

Gene locus TTHA1042 in contig AP008226_GR.

KEGG

ttj:TTHA1042.

NMPDR

fig|300852.3.peg.1179.

PATRIC

23957044. VBITheThe93045_1022.

Phylogenomic databases

eggNOG

COG0782.

HOGENOM

HBG753387.

OMA

QMETSAD.

ProtClustDB

CLSK2761922.

Enzyme and pathway databases

BioCyc

TTHE300852:TTHA1042-MONOMER.

Family and domain databases

InterPro

IPR018151. TF_GreA/GreB_CS.
IPR023459. Tscrpt_elong_fac_GreA/B.
IPR001437. Tscrpt_elong_fac_GreA/B_C.
IPR022691. Tscrpt_elong_fac_GreA/B_N.
[Graphical view]

Gene3D

G3DSA:1.10.287.180. GreA_GreB_reg. 1 hit.
G3DSA:3.10.50.30. GreA_GreB_reg. 1 hit.

KO

K03624.

Pfam

PF01272. GreA_GreB. 1 hit.
PF03449. GreA_GreB_N. 1 hit.
[Graphical view]

PIRSF

PIRSF006092. GreA_GreB. 1 hit.

SUPFAM

SSF46557. GreA_GreB. 1 hit.

PROSITE

PS00830. GREAB_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

Q5SJG6_THET8

Accession

Primary (citable) accession number: Q5SJG6

Entry history

Integrated into UniProtKB/TrEMBL:	December 21, 2004
Last sequence update:	December 21, 2004
Last modified:	December 14, 2011
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)