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Protein

Transcription inhibitor protein Gfh1

Gene

gfh1

Organism
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Inhibits all catalytic activities of RNA polymerase (RNAP) by partially occluding its substrate-binding site and preventing NTP binding.3 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi20Zinc1 Publication1
Metal bindingi24Zinc1 Publication1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processTranscription, Transcription regulation
LigandMetal-binding, Zinc

Enzyme and pathway databases

BioCyciTTHE300852:G1GKC-1049-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription inhibitor protein Gfh1
Alternative name(s):
Anti-cleavage anti-GreA transcription factor
Gre factor homolog 1
Gene namesi
Name:gfh1
Ordered Locus Names:TTHA1042
OrganismiThermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Taxonomic identifieri300852 [NCBI]
Taxonomic lineageiBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus
Proteomesi
  • UP000000532 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004222491 – 156Transcription inhibitor protein Gfh1Add BLAST156

Interactioni

Subunit structurei

Interacts with RNAP.4 Publications

GO - Molecular functioni

Protein-protein interaction databases

DIPiDIP-59216N
IntActiQ5SJG6, 4 interactors
STRINGi300852.TTHA1042

Structurei

Secondary structure

1156
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi5 – 7Combined sources3
Helixi9 – 38Combined sources30
Helixi46 – 71Combined sources26
Beta strandi72 – 74Combined sources3
Beta strandi88 – 93Combined sources6
Turni94 – 97Combined sources4
Beta strandi98 – 105Combined sources8
Turni107 – 109Combined sources3
Beta strandi114 – 116Combined sources3
Beta strandi118 – 120Combined sources3
Helixi124 – 129Combined sources6
Beta strandi137 – 140Combined sources4
Beta strandi147 – 154Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2EULX-ray2.40A/B/C/D1-156[»]
3AOHX-ray4.10X/Y/Z1-156[»]
3AOIX-ray4.30X/Y/Z1-156[»]
4WQTX-ray4.40X/Y/Z1-156[»]
ProteinModelPortaliQ5SJG6
SMRiQ5SJG6
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ5SJG6

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili1 – 74Sequence analysisAdd BLAST74

Domaini

Inhibitory activity is regulated via a pH-induced conformational change of the structure. At pH above 7, Gfh1 is in an inactive flipped orientation that prevents binding to RNAP. At lower pH, Gfh1 switches to an active orientation, which enables binding to RNAP and inhibitory activity (By similarity).By similarity

Sequence similaritiesi

Belongs to the GreA/GreB family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG4105RTX Bacteria
COG0782 LUCA
HOGENOMiHOG000241145
KOiK03624
OMAiNSEYHSA
PhylomeDBiQ5SJG6

Family and domain databases

Gene3Di1.10.287.180, 1 hit
3.10.50.30, 1 hit
InterProiView protein in InterPro
IPR036953 GreA/GreB_C_sf
IPR018151 TF_GreA/GreB_CS
IPR001437 Tscrpt_elong_fac_GreA/B_C
IPR023459 Tscrpt_elong_fac_GreA/B_fam
IPR022691 Tscrpt_elong_fac_GreA/B_N
IPR036805 Tscrpt_elong_fac_GreA/B_N_sf
PANTHERiPTHR30437 PTHR30437, 1 hit
PfamiView protein in Pfam
PF01272 GreA_GreB, 1 hit
PF03449 GreA_GreB_N, 1 hit
PIRSFiPIRSF006092 GreA_GreB, 1 hit
SUPFAMiSSF46557 SSF46557, 1 hit
PROSITEiView protein in PROSITE
PS00830 GREAB_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q5SJG6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAREVKLTKA GYERLMQQLE RERERLQEAT KILQELMESS DDYDDSGLEA
60 70 80 90 100
AKQEKARIEA RIDSLEDILS RAVILEEGSG EVIGLGSVVE LEDPLSGERL
110 120 130 140 150
SVQVVSPAEA NVLDTPMKIS DASPMGKALL GHRVGDVLSL DTPKGRREFR

VVAIHG
Length:156
Mass (Da):17,183
Last modified:December 21, 2004 - v1
Checksum:i2B7BB05B0E8ECE14
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti146R → K in AAL57612 (PubMed:11606592).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF456235 Genomic DNA Translation: AAL57612.1
AP008226 Genomic DNA Translation: BAD70865.1
RefSeqiWP_011228396.1, NC_006461.1
YP_144308.1, NC_006461.1

Genome annotation databases

EnsemblBacteriaiBAD70865; BAD70865; BAD70865
GeneIDi3168268
KEGGittj:TTHA1042
PATRICifig|300852.9.peg.1022

Similar proteinsi

Entry informationi

Entry nameiGFH1_THET8
AccessioniPrimary (citable) accession number: Q5SJG6
Secondary accession number(s): Q8VQD5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 1, 2013
Last sequence update: December 21, 2004
Last modified: April 25, 2018
This is version 100 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

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