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Protein

Shikimate dehydrogenase (NADP(+))

Gene

aroE

Organism
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in the biosynthesis of the chorismate, which leads to the biosynthesis of aromatic amino acids. Catalyzes the reversible NADPH linked reduction of 3-dehydroshikimate (DHSA) to yield shikimate (SA).UniRule annotation

Catalytic activityi

Shikimate + NADP+ = 3-dehydroshikimate + NADPH.UniRule annotation

Pathwayi: chorismate biosynthesis

This protein is involved in step 4 of the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate.UniRule annotation
Proteins known to be involved in the 7 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. 3-dehydroquinate synthase (aroB)
  3. 3-dehydroquinate dehydratase (aroQ)
  4. Shikimate dehydrogenase (NADP(+)) (aroE)
  5. Shikimate kinase (aroK)
  6. 3-phosphoshikimate 1-carboxyvinyltransferase (aroA)
  7. Chorismate synthase (aroC)
This subpathway is part of the pathway chorismate biosynthesis, which is itself part of Metabolic intermediate biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate, the pathway chorismate biosynthesis and in Metabolic intermediate biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei60ShikimateUniRule annotation1 Publication1
Active sitei64Proton acceptorUniRule annotation1 Publication1
Binding sitei85ShikimateUniRule annotation1 Publication1
Binding sitei100ShikimateUniRule annotation1 Publication1
Binding sitei205NADP; via carbonyl oxygenUniRule annotation1 Publication1
Binding sitei207ShikimateUniRule annotation1 Publication1
Binding sitei228NADP; via carbonyl oxygenUniRule annotation1 Publication1
Binding sitei235ShikimateUniRule annotation1 Publication1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi123 – 127NADPUniRule annotation1 Publication5
Nucleotide bindingi146 – 151NADPUniRule annotation1 Publication6

GO - Molecular functioni

  • NADP binding Source: UniProtKB
  • shikimate 3-dehydrogenase (NADP+) activity Source: UniProtKB-EC

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processAmino-acid biosynthesis, Aromatic amino acid biosynthesis
LigandNADP

Enzyme and pathway databases

BioCyciTTHE300852:G1GKC-1057-MONOMER
BRENDAi1.1.1.25 2305
UniPathwayiUPA00053; UER00087

Names & Taxonomyi

Protein namesi
Recommended name:
Shikimate dehydrogenase (NADP(+))UniRule annotation (EC:1.1.1.25UniRule annotation)
Short name:
SDHUniRule annotation
Gene namesi
Name:aroEUniRule annotation
Ordered Locus Names:TTHA1050
OrganismiThermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Taxonomic identifieri300852 [NCBI]
Taxonomic lineageiBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus
Proteomesi
  • UP000000532 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000781331 – 263Shikimate dehydrogenase (NADP(+))Add BLAST263

Interactioni

Subunit structurei

Homodimer.UniRule annotation1 Publication

Protein-protein interaction databases

STRINGi300852.TTHA1050

Structurei

Secondary structure

1263
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi2 – 10Combined sources9
Helixi16 – 26Combined sources11
Beta strandi31 – 37Combined sources7
Helixi40 – 42Combined sources3
Helixi43 – 53Combined sources11
Beta strandi55 – 59Combined sources5
Turni61 – 65Combined sources5
Helixi66 – 70Combined sources5
Beta strandi72 – 74Combined sources3
Helixi76 – 81Combined sources6
Beta strandi86 – 90Combined sources5
Beta strandi93 – 97Combined sources5
Helixi100 – 110Combined sources11
Beta strandi119 – 122Combined sources4
Helixi126 – 137Combined sources12
Beta strandi142 – 145Combined sources4
Helixi149 – 159Combined sources11
Helixi166 – 171Combined sources6
Beta strandi173 – 177Combined sources5
Turni181 – 184Combined sources4
Helixi193 – 195Combined sources3
Beta strandi198 – 205Combined sources8
Beta strandi208 – 211Combined sources4
Helixi213 – 220Combined sources8
Beta strandi224 – 226Combined sources3
Helixi229 – 244Combined sources16
Helixi250 – 260Combined sources11

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1WXDX-ray2.10A/B1-263[»]
2CY0X-ray1.90A/B1-263[»]
2D5CX-ray1.65A/B1-263[»]
2EV9X-ray1.90A/B1-263[»]
ProteinModelPortaliQ5SJF8
SMRiQ5SJF8
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ5SJF8

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni14 – 16Shikimate bindingUniRule annotation1 Publication3

Sequence similaritiesi

Belongs to the shikimate dehydrogenase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105E2X Bacteria
COG0169 LUCA
HOGENOMiHOG000237875
KOiK00014
OMAiFGNPIKH
PhylomeDBiQ5SJF8

Family and domain databases

HAMAPiMF_00222 Shikimate_DH_AroE, 1 hit
InterProiView protein in InterPro
IPR036291 NAD(P)-bd_dom_sf
IPR011342 Shikimate_DH
IPR013708 Shikimate_DH-bd_N
IPR022893 Shikimate_DH_fam
IPR006151 Shikm_DH/Glu-tRNA_Rdtase
PfamiView protein in Pfam
PF01488 Shikimate_DH, 1 hit
PF08501 Shikimate_dh_N, 1 hit
SUPFAMiSSF51735 SSF51735, 1 hit
TIGRFAMsiTIGR00507 aroE, 1 hit

Sequencei

Sequence statusi: Complete.

Q5SJF8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLRFAVLGHP VAHSLSPAMH AFALESLGLE GSYEAWDTPL EALPGRLKEV
60 70 80 90 100
RRAFRGVNLT LPLKEAALAH LDWVSPEAQR IGAVNTVLQV EGRLFGFNTD
110 120 130 140 150
APGFLEALKA GGIPLKGPAL VLGAGGAGRA VAFALREAGL EVWVWNRTPQ
160 170 180 190 200
RALALAEEFG LRAVPLEKAR EARLLVNATR VGLEDPSASP LPAELFPEEG
210 220 230 240 250
AAVDLVYRPL WTRFLREAKA KGLKVQTGLP MLAWQGALAF RLWTGLLPDP
260
SGMEEAARRA LGV
Length:263
Mass (Da):28,284
Last modified:December 21, 2004 - v1
Checksum:iBF53F1D49A1A82A4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP008226 Genomic DNA Translation: BAD70873.1
RefSeqiWP_011228401.1, NC_006461.1
YP_144316.1, NC_006461.1

Genome annotation databases

EnsemblBacteriaiBAD70873; BAD70873; BAD70873
GeneIDi3169915
KEGGittj:TTHA1050
PATRICifig|300852.9.peg.1030

Similar proteinsi

Entry informationi

Entry nameiAROE_THET8
AccessioniPrimary (citable) accession number: Q5SJF8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: December 21, 2004
Last modified: March 28, 2018
This is version 100 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
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Main funding by: National Institutes of Health