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Protein

Shikimate dehydrogenase (NADP(+))

Gene

aroE

Organism
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the biosynthesis of the chorismate, which leads to the biosynthesis of aromatic amino acids. Catalyzes the reversible NADPH linked reduction of 3-dehydroshikimate (DHSA) to yield shikimate (SA).UniRule annotation

Catalytic activityi

Shikimate + NADP+ = 3-dehydroshikimate + NADPH.UniRule annotation

Pathwayi: chorismate biosynthesis

This protein is involved in step 4 of the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate.UniRule annotation
Proteins known to be involved in the 7 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. 3-dehydroquinate synthase (aroB)
  3. 3-dehydroquinate dehydratase (aroQ)
  4. Shikimate dehydrogenase (NADP(+)) (aroE)
  5. Shikimate kinase (aroK)
  6. 3-phosphoshikimate 1-carboxyvinyltransferase (aroA)
  7. Chorismate synthase (aroC)
This subpathway is part of the pathway chorismate biosynthesis, which is itself part of Metabolic intermediate biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate, the pathway chorismate biosynthesis and in Metabolic intermediate biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei60ShikimateUniRule annotation1 Publication1
Active sitei64Proton acceptorUniRule annotation1 Publication1
Binding sitei85ShikimateUniRule annotation1 Publication1
Binding sitei100ShikimateUniRule annotation1 Publication1
Binding sitei205NADP; via carbonyl oxygenUniRule annotation1 Publication1
Binding sitei207ShikimateUniRule annotation1 Publication1
Binding sitei228NADP; via carbonyl oxygenUniRule annotation1 Publication1
Binding sitei235ShikimateUniRule annotation1 Publication1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi123 – 127NADPUniRule annotation1 Publication5
Nucleotide bindingi146 – 151NADPUniRule annotation1 Publication6

GO - Molecular functioni

  • NADP binding Source: UniProtKB
  • shikimate 3-dehydrogenase (NADP+) activity Source: UniProtKB-HAMAP

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Amino-acid biosynthesis, Aromatic amino acid biosynthesis

Keywords - Ligandi

NADP

Enzyme and pathway databases

BRENDAi1.1.1.25. 2305.
UniPathwayiUPA00053; UER00087.

Names & Taxonomyi

Protein namesi
Recommended name:
Shikimate dehydrogenase (NADP(+))UniRule annotation (EC:1.1.1.25UniRule annotation)
Short name:
SDHUniRule annotation
Gene namesi
Name:aroEUniRule annotation
Ordered Locus Names:TTHA1050
OrganismiThermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Taxonomic identifieri300852 [NCBI]
Taxonomic lineageiBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus
Proteomesi
  • UP000000532 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000781331 – 263Shikimate dehydrogenase (NADP(+))Add BLAST263

Interactioni

Subunit structurei

Homodimer.UniRule annotation1 Publication

Protein-protein interaction databases

STRINGi300852.TTHA1050.

Structurei

Secondary structure

1263
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi2 – 10Combined sources9
Helixi16 – 26Combined sources11
Beta strandi31 – 37Combined sources7
Helixi40 – 42Combined sources3
Helixi43 – 53Combined sources11
Beta strandi55 – 59Combined sources5
Turni61 – 65Combined sources5
Helixi66 – 70Combined sources5
Beta strandi72 – 74Combined sources3
Helixi76 – 81Combined sources6
Beta strandi86 – 90Combined sources5
Beta strandi93 – 97Combined sources5
Helixi100 – 110Combined sources11
Beta strandi119 – 122Combined sources4
Helixi126 – 137Combined sources12
Beta strandi142 – 145Combined sources4
Helixi149 – 159Combined sources11
Helixi166 – 171Combined sources6
Beta strandi173 – 177Combined sources5
Turni181 – 184Combined sources4
Helixi193 – 195Combined sources3
Beta strandi198 – 205Combined sources8
Beta strandi208 – 211Combined sources4
Helixi213 – 220Combined sources8
Beta strandi224 – 226Combined sources3
Helixi229 – 244Combined sources16
Helixi250 – 260Combined sources11

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1WXDX-ray2.10A/B1-263[»]
2CY0X-ray1.90A/B1-263[»]
2D5CX-ray1.65A/B1-263[»]
2EV9X-ray1.90A/B1-263[»]
ProteinModelPortaliQ5SJF8.
SMRiQ5SJF8.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ5SJF8.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni14 – 16Shikimate bindingUniRule annotation1 Publication3

Sequence similaritiesi

Belongs to the shikimate dehydrogenase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105E2X. Bacteria.
COG0169. LUCA.
HOGENOMiHOG000237875.
KOiK00014.
OMAiSIYIDAT.
PhylomeDBiQ5SJF8.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
HAMAPiMF_00222. Shikimate_DH_AroE. 1 hit.
InterProiIPR016040. NAD(P)-bd_dom.
IPR011342. Shikimate_DH.
IPR013708. Shikimate_DH-bd_N.
IPR022893. Shikimate_DH_fam.
IPR006151. Shikm_DH/Glu-tRNA_Rdtase.
[Graphical view]
PfamiPF01488. Shikimate_DH. 1 hit.
PF08501. Shikimate_dh_N. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR00507. aroE. 1 hit.

Sequencei

Sequence statusi: Complete.

Q5SJF8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLRFAVLGHP VAHSLSPAMH AFALESLGLE GSYEAWDTPL EALPGRLKEV
60 70 80 90 100
RRAFRGVNLT LPLKEAALAH LDWVSPEAQR IGAVNTVLQV EGRLFGFNTD
110 120 130 140 150
APGFLEALKA GGIPLKGPAL VLGAGGAGRA VAFALREAGL EVWVWNRTPQ
160 170 180 190 200
RALALAEEFG LRAVPLEKAR EARLLVNATR VGLEDPSASP LPAELFPEEG
210 220 230 240 250
AAVDLVYRPL WTRFLREAKA KGLKVQTGLP MLAWQGALAF RLWTGLLPDP
260
SGMEEAARRA LGV
Length:263
Mass (Da):28,284
Last modified:December 21, 2004 - v1
Checksum:iBF53F1D49A1A82A4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP008226 Genomic DNA. Translation: BAD70873.1.
RefSeqiWP_011228401.1. NC_006461.1.
YP_144316.1. NC_006461.1.

Genome annotation databases

EnsemblBacteriaiBAD70873; BAD70873; BAD70873.
GeneIDi3169915.
KEGGittj:TTHA1050.
PATRICi23957060. VBITheThe93045_1030.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP008226 Genomic DNA. Translation: BAD70873.1.
RefSeqiWP_011228401.1. NC_006461.1.
YP_144316.1. NC_006461.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1WXDX-ray2.10A/B1-263[»]
2CY0X-ray1.90A/B1-263[»]
2D5CX-ray1.65A/B1-263[»]
2EV9X-ray1.90A/B1-263[»]
ProteinModelPortaliQ5SJF8.
SMRiQ5SJF8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi300852.TTHA1050.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAD70873; BAD70873; BAD70873.
GeneIDi3169915.
KEGGittj:TTHA1050.
PATRICi23957060. VBITheThe93045_1030.

Phylogenomic databases

eggNOGiENOG4105E2X. Bacteria.
COG0169. LUCA.
HOGENOMiHOG000237875.
KOiK00014.
OMAiSIYIDAT.
PhylomeDBiQ5SJF8.

Enzyme and pathway databases

UniPathwayiUPA00053; UER00087.
BRENDAi1.1.1.25. 2305.

Miscellaneous databases

EvolutionaryTraceiQ5SJF8.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
HAMAPiMF_00222. Shikimate_DH_AroE. 1 hit.
InterProiIPR016040. NAD(P)-bd_dom.
IPR011342. Shikimate_DH.
IPR013708. Shikimate_DH-bd_N.
IPR022893. Shikimate_DH_fam.
IPR006151. Shikm_DH/Glu-tRNA_Rdtase.
[Graphical view]
PfamiPF01488. Shikimate_DH. 1 hit.
PF08501. Shikimate_dh_N. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR00507. aroE. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiAROE_THET8
AccessioniPrimary (citable) accession number: Q5SJF8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: December 21, 2004
Last modified: November 2, 2016
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.