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Q5SJC4

- RUVC_THET8

UniProt

Q5SJC4 - RUVC_THET8

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Protein

Crossover junction endodeoxyribonuclease RuvC

Gene
ruvC, TTHA1090
Organism
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leaves a 5'-terminal phosphate and a 3'-terminal hydroxyl group By similarity.UniRule annotation

Catalytic activityi

Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction).UniRule annotation

Cofactori

Binds 1 magnesium ion per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi7 – 71Magnesium By similarity
Metal bindingi70 – 701Magnesium By similarity
Metal bindingi143 – 1431Magnesium By similarity
Metal bindingi146 – 1461Magnesium By similarity

GO - Molecular functioni

  1. crossover junction endodeoxyribonuclease activity Source: UniProtKB-HAMAP
  2. magnesium ion binding Source: UniProtKB-HAMAP
  3. nucleic acid binding Source: InterPro

GO - Biological processi

  1. DNA recombination Source: UniProtKB-HAMAP
  2. DNA repair Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Nuclease

Keywords - Biological processi

DNA damage, DNA recombination, DNA repair

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciTTHE300852:GH8R-1122-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Crossover junction endodeoxyribonuclease RuvC (EC:3.1.22.4)
Alternative name(s):
Holliday junction nuclease RuvC
Holliday junction resolvase RuvC
Gene namesi
Name:ruvC
Ordered Locus Names:TTHA1090
OrganismiThermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Taxonomic identifieri300852 [NCBI]
Taxonomic lineageiBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus
ProteomesiUP000000532: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 166166Crossover junction endodeoxyribonuclease RuvCUniRule annotationPRO_0000225184Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi300852.TTHA1090.

Structurei

Secondary structure

1
166
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi2 – 76
Beta strandi10 – 2011
Beta strandi22 – 265
Beta strandi28 – 3710
Beta strandi40 – 423
Helixi44 – 6219
Beta strandi65 – 706
Helixi79 – 9820
Beta strandi102 – 1054
Helixi107 – 1159
Helixi122 – 13211
Helixi142 – 15918

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4EP4X-ray1.28A/B1-166[»]
4EP5X-ray2.08A1-166[»]
4LD0X-ray3.75A/B1-166[»]
ProteinModelPortaliQ5SJC4.

Family & Domainsi

Sequence similaritiesi

Belongs to the RuvC family.

Phylogenomic databases

eggNOGiCOG0817.
HOGENOMiHOG000012182.
KOiK01159.
OMAiCIRTASD.
OrthoDBiEOG6RG044.
PhylomeDBiQ5SJC4.

Family and domain databases

Gene3Di3.30.420.10. 1 hit.
HAMAPiMF_00034. RuvC.
InterProiIPR012337. RNaseH-like_dom.
IPR002176. X-over_junc_endoDNase_RuvC.
[Graphical view]
PfamiPF02075. RuvC. 1 hit.
[Graphical view]
PRINTSiPR00696. RSOLVASERUVC.
SUPFAMiSSF53098. SSF53098. 1 hit.
TIGRFAMsiTIGR00228. ruvC. 1 hit.

Sequencei

Sequence statusi: Complete.

Q5SJC4-1 [UniParc]FASTAAdd to Basket

« Hide

MVVAGIDPGI THLGLGVVAV EGKGALKARL LHGEVVKTSP QEPAKERVGR    50
IHARVLEVLH RFRPEAVAVE EQFFYRQNEL AYKVGWALGA VLVAAFEAGV 100
PVYAYGPMQV KQALAGHGHA AKEEVALMVR GILGLKEAPR PSHLADALAI 150
ALTHAFYARM GTAKPL 166
Length:166
Mass (Da):17,755
Last modified:December 21, 2004 - v1
Checksum:i214FBE6E1D4A91C3
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AP008226 Genomic DNA. Translation: BAD70913.1.
RefSeqiYP_144356.1. NC_006461.1.

Genome annotation databases

EnsemblBacteriaiBAD70913; BAD70913; BAD70913.
GeneIDi3168165.
KEGGittj:TTHA1090.
PATRICi23957140. VBITheThe93045_1070.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AP008226 Genomic DNA. Translation: BAD70913.1 .
RefSeqi YP_144356.1. NC_006461.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
4EP4 X-ray 1.28 A/B 1-166 [» ]
4EP5 X-ray 2.08 A 1-166 [» ]
4LD0 X-ray 3.75 A/B 1-166 [» ]
ProteinModelPortali Q5SJC4.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 300852.TTHA1090.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAD70913 ; BAD70913 ; BAD70913 .
GeneIDi 3168165.
KEGGi ttj:TTHA1090.
PATRICi 23957140. VBITheThe93045_1070.

Phylogenomic databases

eggNOGi COG0817.
HOGENOMi HOG000012182.
KOi K01159.
OMAi CIRTASD.
OrthoDBi EOG6RG044.
PhylomeDBi Q5SJC4.

Enzyme and pathway databases

BioCyci TTHE300852:GH8R-1122-MONOMER.

Family and domain databases

Gene3Di 3.30.420.10. 1 hit.
HAMAPi MF_00034. RuvC.
InterProi IPR012337. RNaseH-like_dom.
IPR002176. X-over_junc_endoDNase_RuvC.
[Graphical view ]
Pfami PF02075. RuvC. 1 hit.
[Graphical view ]
PRINTSi PR00696. RSOLVASERUVC.
SUPFAMi SSF53098. SSF53098. 1 hit.
TIGRFAMsi TIGR00228. ruvC. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Complete genome sequence of Thermus thermophilus HB8."
    Masui R., Kurokawa K., Nakagawa N., Tokunaga F., Koyama Y., Shibata T., Oshima T., Yokoyama S., Yasunaga T., Kuramitsu S.
    Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: HB8 / ATCC 27634 / DSM 579.

Entry informationi

Entry nameiRUVC_THET8
AccessioniPrimary (citable) accession number: Q5SJC4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 7, 2006
Last sequence update: December 21, 2004
Last modified: July 9, 2014
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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