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Protein

L-lactate dehydrogenase

Gene

ldh

Organism
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

(S)-lactate + NAD+ = pyruvate + NADH.UniRule annotation

Pathwayi: pyruvate fermentation to lactate

This protein is involved in step 1 of the subpathway that synthesizes (S)-lactate from pyruvate.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. L-lactate dehydrogenase (ldh)
This subpathway is part of the pathway pyruvate fermentation to lactate, which is itself part of Fermentation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-lactate from pyruvate, the pathway pyruvate fermentation to lactate and in Fermentation.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei85 – 851SubstrateUniRule annotation
Binding sitei117 – 1171NAD or substrateUniRule annotation
Binding sitei148 – 1481SubstrateUniRule annotation
Active sitei172 – 1721Proton acceptor
Binding sitei227 – 2271SubstrateUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi9 – 3729NADUniRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NAD

Enzyme and pathway databases

BioCyciTTHE300852:GH8R-1145-MONOMER.
BRENDAi1.1.1.27. 2305.
UniPathwayiUPA00554; UER00611.

Names & Taxonomyi

Protein namesi
Recommended name:
L-lactate dehydrogenaseUniRule annotation (EC:1.1.1.27UniRule annotation)
Short name:
L-LDHUniRule annotation
Gene namesi
Name:ldhUniRule annotation
Ordered Locus Names:TTHA1113
OrganismiThermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Taxonomic identifieri300852 [NCBI]
Taxonomic lineageiBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus
Proteomesi
  • UP000000532 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 310310L-lactate dehydrogenasePRO_0000237569Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei218 – 2181PhosphotyrosineUniRule annotation

Keywords - PTMi

Phosphoprotein

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi300852.TTHA1113.

Structurei

Secondary structure

1
310
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi2 – 65Combined sources
Helixi10 – 2112Combined sources
Beta strandi26 – 316Combined sources
Helixi35 – 4612Combined sources
Helixi47 – 515Combined sources
Beta strandi56 – 594Combined sources
Helixi62 – 654Combined sources
Beta strandi68 – 736Combined sources
Helixi85 – 10622Combined sources
Beta strandi111 – 1144Combined sources
Beta strandi116 – 1183Combined sources
Helixi119 – 13012Combined sources
Helixi134 – 1363Combined sources
Beta strandi137 – 1393Combined sources
Helixi143 – 15715Combined sources
Helixi161 – 1633Combined sources
Beta strandi164 – 1707Combined sources
Beta strandi177 – 1859Combined sources
Helixi190 – 1967Combined sources
Helixi203 – 21412Combined sources
Helixi216 – 2249Combined sources
Helixi229 – 24315Combined sources
Beta strandi248 – 25912Combined sources
Beta strandi262 – 27413Combined sources
Beta strandi277 – 2815Combined sources
Helixi288 – 30821Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2E37X-ray2.30A/B/C/D/E/F/G/H1-310[»]
2V6MX-ray2.20A/B/C/D1-310[»]
2V7PX-ray2.10A/B/C/D1-310[»]
2XXBX-ray2.15A/B1-310[»]
2XXJX-ray1.96A/B/C/D1-310[»]
3ZZNX-ray2.90A/B/C/D1-310[»]
4A73X-ray3.00A/B/C/D1-310[»]
ProteinModelPortaliQ5SJA1.
SMRiQ5SJA1. Positions 1-310.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ5SJA1.

Family & Domainsi

Sequence similaritiesi

Belongs to the LDH/MDH superfamily. LDH family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C80. Bacteria.
COG0039. LUCA.
HOGENOMiHOG000213793.
KOiK00016.
OMAiAGDYEDC.
OrthoDBiEOG6091FG.
PhylomeDBiQ5SJA1.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPiMF_00488. Lactate_dehydrog.
InterProiIPR001557. L-lactate/malate_DH.
IPR011304. L-lactate_DH.
IPR018177. L-lactate_DH_AS.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11540. PTHR11540. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
PRINTSiPR00086. LLDHDRGNASE.
SUPFAMiSSF51735. SSF51735. 1 hit.
SSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01771. L-LDH-NAD. 1 hit.
PROSITEiPS00064. L_LDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5SJA1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKVGIVGSGM VGSATAYALA LLGVAREVVL VDLDRKLAQA HAEDILHATP
60 70 80 90 100
FAHPVWVRAG SYGDLEGARA VVLAAGVAQR PGETRLQLLD RNAQVFAQVV
110 120 130 140 150
PRVLEAAPEA VLLVATNPVD VMTQVAYRLS GLPPGRVVGS GTILDTARFR
160 170 180 190 200
ALLAEYLRVA PQSVHAYVLG EHGDSEVLVW SSAQVGGVPL LEFAEARGRA
210 220 230 240 250
LSPEDRARID EGVRRAAYRI IEGKGATYYG IGAGLARLVR AILTDEKGVY
260 270 280 290 300
TVSAFTPEVE GVLEVSLSLP RILGAGGVEG TVYPSLSPEE REALRRSAEI
310
LKEAAFALGF
Length:310
Mass (Da):32,795
Last modified:December 21, 2004 - v1
Checksum:i71D12243E8C00DE4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP008226 Genomic DNA. Translation: BAD70936.1.
RefSeqiWP_011228449.1. NC_006461.1.
YP_144379.1. NC_006461.1.

Genome annotation databases

EnsemblBacteriaiBAD70936; BAD70936; BAD70936.
GeneIDi3169622.
KEGGittj:TTHA1113.
PATRICi23957185. VBITheThe93045_1092.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP008226 Genomic DNA. Translation: BAD70936.1.
RefSeqiWP_011228449.1. NC_006461.1.
YP_144379.1. NC_006461.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2E37X-ray2.30A/B/C/D/E/F/G/H1-310[»]
2V6MX-ray2.20A/B/C/D1-310[»]
2V7PX-ray2.10A/B/C/D1-310[»]
2XXBX-ray2.15A/B1-310[»]
2XXJX-ray1.96A/B/C/D1-310[»]
3ZZNX-ray2.90A/B/C/D1-310[»]
4A73X-ray3.00A/B/C/D1-310[»]
ProteinModelPortaliQ5SJA1.
SMRiQ5SJA1. Positions 1-310.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi300852.TTHA1113.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAD70936; BAD70936; BAD70936.
GeneIDi3169622.
KEGGittj:TTHA1113.
PATRICi23957185. VBITheThe93045_1092.

Phylogenomic databases

eggNOGiENOG4105C80. Bacteria.
COG0039. LUCA.
HOGENOMiHOG000213793.
KOiK00016.
OMAiAGDYEDC.
OrthoDBiEOG6091FG.
PhylomeDBiQ5SJA1.

Enzyme and pathway databases

UniPathwayiUPA00554; UER00611.
BioCyciTTHE300852:GH8R-1145-MONOMER.
BRENDAi1.1.1.27. 2305.

Miscellaneous databases

EvolutionaryTraceiQ5SJA1.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPiMF_00488. Lactate_dehydrog.
InterProiIPR001557. L-lactate/malate_DH.
IPR011304. L-lactate_DH.
IPR018177. L-lactate_DH_AS.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11540. PTHR11540. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
PRINTSiPR00086. LLDHDRGNASE.
SUPFAMiSSF51735. SSF51735. 1 hit.
SSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01771. L-LDH-NAD. 1 hit.
PROSITEiPS00064. L_LDH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Complete genome sequence of Thermus thermophilus HB8."
    Masui R., Kurokawa K., Nakagawa N., Tokunaga F., Koyama Y., Shibata T., Oshima T., Yokoyama S., Yasunaga T., Kuramitsu S.
    Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: HB8 / ATCC 27634 / DSM 579.

Entry informationi

Entry nameiLDH_THET8
AccessioniPrimary (citable) accession number: Q5SJA1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2006
Last sequence update: December 21, 2004
Last modified: December 9, 2015
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.