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Protein

L-lactate dehydrogenase

Gene

ldh

Organism
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the conversion of lactate to pyruvate.UniRule annotation1 Publication

Catalytic activityi

(S)-lactate + NAD+ = pyruvate + NADH.UniRule annotation1 Publication

Enzyme regulationi

Allosterically activated by fructose 1,6-bisphosphate (FBP).UniRule annotation

Kineticsi

Kcat is 676 sec(-1) for pyruvate as substrate.1 Publication
  1. KM=0.16 mM for pyruvate1 Publication

    Temperature dependencei

    Optimum temperature is close to 90 degrees Celsius.1 Publication

    Pathwayi: pyruvate fermentation to lactate

    This protein is involved in step 1 of the subpathway that synthesizes (S)-lactate from pyruvate.UniRule annotation
    Proteins known to be involved in this subpathway in this organism are:
    1. L-lactate dehydrogenase (ldh)
    This subpathway is part of the pathway pyruvate fermentation to lactate, which is itself part of Fermentation.
    View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-lactate from pyruvate, the pathway pyruvate fermentation to lactate and in Fermentation.

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Binding sitei32NADUniRule annotationCombined sources1 Publication3 Publications1
    Binding sitei62NADUniRule annotationCombined sources2 Publications1
    Binding sitei79SubstrateUniRule annotationCombined sources2 Publications1
    Binding sitei85SubstrateCombined sources2 Publications1
    Binding sitei140NADCombined sources2 Publications1
    Binding sitei150Allosteric activatorUniRule annotation1
    Binding sitei165Allosteric activatorUniRule annotation1
    Active sitei172Proton acceptorUniRule annotationCombined sources2 Publications1
    Binding sitei227SubstrateCombined sources2 Publications1

    Regions

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Nucleotide bindingi10 – 11NADUniRule annotationCombined sources1 Publication3 Publications2
    Nucleotide bindingi76 – 77NADUniRule annotationCombined sources1 Publication3 Publications2
    Nucleotide bindingi115 – 117NADUniRule annotationCombined sources2 Publications3

    GO - Molecular functioni

    GO - Biological processi

    Keywordsi

    Molecular functionAllosteric enzyme, Oxidoreductase
    LigandNAD

    Enzyme and pathway databases

    BioCyciTTHE300852:G1GKC-1119-MONOMER
    BRENDAi1.1.1.27 2305
    UniPathwayiUPA00554; UER00611

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    L-lactate dehydrogenase1 PublicationUniRule annotation (EC:1.1.1.27UniRule annotation1 Publication)
    Short name:
    L-LDH1 PublicationUniRule annotation
    Gene namesi
    Name:ldhUniRule annotation
    Ordered Locus Names:TTHA1113
    OrganismiThermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
    Taxonomic identifieri300852 [NCBI]
    Taxonomic lineageiBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus
    Proteomesi

    Subcellular locationi

    • Cytoplasm UniRule annotation

    GO - Cellular componenti

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00002375691 – 310L-lactate dehydrogenaseAdd BLAST310

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Modified residuei218PhosphotyrosineUniRule annotation1

    Keywords - PTMi

    Phosphoprotein

    Interactioni

    Subunit structurei

    Homotetramer.UniRule annotation2 Publications2 Publications

    Protein-protein interaction databases

    STRINGi300852.TTHA1113

    Structurei

    Secondary structure

    1310
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details
    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Beta strandi2 – 6Combined sources5
    Helixi10 – 21Combined sources12
    Beta strandi26 – 31Combined sources6
    Helixi35 – 46Combined sources12
    Helixi47 – 51Combined sources5
    Beta strandi56 – 59Combined sources4
    Helixi62 – 65Combined sources4
    Beta strandi68 – 73Combined sources6
    Helixi85 – 106Combined sources22
    Beta strandi111 – 114Combined sources4
    Beta strandi116 – 118Combined sources3
    Helixi119 – 130Combined sources12
    Helixi134 – 136Combined sources3
    Beta strandi137 – 139Combined sources3
    Helixi143 – 157Combined sources15
    Helixi161 – 163Combined sources3
    Beta strandi164 – 170Combined sources7
    Beta strandi177 – 185Combined sources9
    Helixi190 – 196Combined sources7
    Helixi203 – 214Combined sources12
    Helixi216 – 224Combined sources9
    Helixi229 – 243Combined sources15
    Beta strandi248 – 259Combined sources12
    Beta strandi262 – 274Combined sources13
    Beta strandi277 – 281Combined sources5
    Helixi288 – 308Combined sources21

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    2E37X-ray2.30A/B/C/D/E/F/G/H1-310[»]
    2V6MX-ray2.20A/B/C/D1-310[»]
    2V7PX-ray2.10A/B/C/D1-310[»]
    2XXBX-ray2.15A/B1-310[»]
    2XXJX-ray1.96A/B/C/D1-310[»]
    3ZZNX-ray2.90A/B/C/D1-310[»]
    4A73X-ray3.00A/B/C/D1-310[»]
    ProteinModelPortaliQ5SJA1
    SMRiQ5SJA1
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ5SJA1

    Family & Domainsi

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Regioni117 – 120Substrate bindingUniRule annotation4
    Regioni145 – 148Substrate bindingUniRule annotationCombined sources2 Publications4

    Sequence similaritiesi

    Belongs to the LDH/MDH superfamily. LDH family.UniRule annotation

    Phylogenomic databases

    eggNOGiENOG4105C80 Bacteria
    COG0039 LUCA
    HOGENOMiHOG000213793
    KOiK00016
    OMAiRQPGQTR
    PhylomeDBiQ5SJA1

    Family and domain databases

    Gene3Di3.90.110.10, 1 hit
    HAMAPiMF_00488 Lactate_dehydrog, 1 hit
    InterProiView protein in InterPro
    IPR001557 L-lactate/malate_DH
    IPR011304 L-lactate_DH
    IPR018177 L-lactate_DH_AS
    IPR022383 Lactate/malate_DH_C
    IPR001236 Lactate/malate_DH_N
    IPR015955 Lactate_DH/Glyco_Ohase_4_C
    IPR036291 NAD(P)-bd_dom_sf
    PfamiView protein in Pfam
    PF02866 Ldh_1_C, 1 hit
    PF00056 Ldh_1_N, 1 hit
    PIRSFiPIRSF000102 Lac_mal_DH, 1 hit
    PRINTSiPR00086 LLDHDRGNASE
    SUPFAMiSSF51735 SSF51735, 1 hit
    SSF56327 SSF56327, 1 hit
    TIGRFAMsiTIGR01771 L-LDH-NAD, 1 hit
    PROSITEiView protein in PROSITE
    PS00064 L_LDH, 1 hit

    Sequencei

    Sequence statusi: Complete.

    Q5SJA1-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MKVGIVGSGM VGSATAYALA LLGVAREVVL VDLDRKLAQA HAEDILHATP
    60 70 80 90 100
    FAHPVWVRAG SYGDLEGARA VVLAAGVAQR PGETRLQLLD RNAQVFAQVV
    110 120 130 140 150
    PRVLEAAPEA VLLVATNPVD VMTQVAYRLS GLPPGRVVGS GTILDTARFR
    160 170 180 190 200
    ALLAEYLRVA PQSVHAYVLG EHGDSEVLVW SSAQVGGVPL LEFAEARGRA
    210 220 230 240 250
    LSPEDRARID EGVRRAAYRI IEGKGATYYG IGAGLARLVR AILTDEKGVY
    260 270 280 290 300
    TVSAFTPEVE GVLEVSLSLP RILGAGGVEG TVYPSLSPEE REALRRSAEI
    310
    LKEAAFALGF
    Length:310
    Mass (Da):32,795
    Last modified:December 21, 2004 - v1
    Checksum:i71D12243E8C00DE4
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AP008226 Genomic DNA Translation: BAD70936.1
    RefSeqiWP_011228449.1, NC_006461.1
    YP_144379.1, NC_006461.1

    Genome annotation databases

    EnsemblBacteriaiBAD70936; BAD70936; BAD70936
    GeneIDi3169622
    KEGGittj:TTHA1113
    PATRICifig|300852.9.peg.1092

    Similar proteinsi

    Entry informationi

    Entry nameiLDH_THET8
    AccessioniPrimary (citable) accession number: Q5SJA1
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2006
    Last sequence update: December 21, 2004
    Last modified: May 23, 2018
    This is version 99 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

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